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Protein

Beta-galactoside alpha-2,6-sialyltransferase 1

Gene

ST6GAL1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.1 Publication

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei195 – 1951Substrate; via amide nitrogenBy similarity
Binding sitei218 – 2181SubstrateBy similarity
Binding sitei239 – 2391SubstrateBy similarity
Binding sitei360 – 3601Substrate; via carbonyl oxygenBy similarity
Binding sitei372 – 3721SubstrateBy similarity
Binding sitei376 – 3761SubstrateBy similarity
Binding sitei377 – 3771SubstrateBy similarity
Binding sitei383 – 3831SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.1. 1306.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.1)
Short name:
Alpha 2,6-ST 1
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
ST6Gal I
Short name:
ST6GalI
Sialyltransferase 1
Gene namesi
Name:ST6GAL1
Synonyms:SIAT1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 2617Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini27 – 413387LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Beta-galactoside alpha-2,6-sialyltransferase 1PRO_0000149252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence analysis
Disulfide bondi148 ↔ 413By similarity
Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence analysis
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi190 ↔ 342By similarity
Disulfide bondi360 ↔ 371By similarity

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ92182.
PRIDEiQ92182.

Expressioni

Tissue specificityi

Highly expressed in testis, brain and liver and to a lesser extent in lung and heart.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000008889.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni329 – 3313Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiQ92182.
KOiK00778.
PhylomeDBiQ92182.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHINVLKKF MCVLVVILIA LTVCLWKETR GSYYVPLKND GTQVHRALDK
60 70 80 90 100
WNLLKSQGLL HEAAGEMGQM PKALPNNQNK VKGITSGAVE KSRKAAEHVK
110 120 130 140 150
VWDKDSSSRN LIPRLQKVRK NYLSMNKYNV TYNGKMNAAK LSPEQLLCRL
160 170 180 190 200
RDRVNVTMIR GSDGPFNSSE WQHYLPDKSL NETVGRLGRC AVVSSAGSLK
210 220 230 240 250
SSHLGPEIDS HDAVLRFNGA PVKGFQEDVG QKTTIRLVNS QLVTVEEQQF
260 270 280 290 300
LKDALYNTGI LIVWDPAPYH AEIHEWYRKP DYKFFEAYKS YRIRHPEQPF
310 320 330 340 350
YILNPKMQWQ LWDILQENSL EHIQPNPPSS GMLGIVIMMT LCDEVDVYEF
360 370 380 390 400
LPSKRQTDIC HYYQKFHDHA CTMGAYHPLL FEKNLVKHLN QGTDEDIYTH
410
GKVTLPGFRN VHC
Length:413
Mass (Da):47,393
Last modified:November 1, 1997 - v1
Checksum:i19B8CCD361ED137D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75558 mRNA. Translation: CAA53235.1.
PIRiS41114.
RefSeqiNP_990572.1. NM_205241.1.
UniGeneiGga.1148.

Genome annotation databases

GeneIDi396169.
KEGGigga:396169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75558 mRNA. Translation: CAA53235.1.
PIRiS41114.
RefSeqiNP_990572.1. NM_205241.1.
UniGeneiGga.1148.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000008889.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Proteomic databases

PaxDbiQ92182.
PRIDEiQ92182.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396169.
KEGGigga:396169.

Organism-specific databases

CTDi6480.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiQ92182.
KOiK00778.
PhylomeDBiQ92182.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.1. 1306.

Miscellaneous databases

NextBioi20816223.
PROiQ92182.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of chick embryo Gal beta 1,4GlcNAc alpha 2,6-sialyltransferase. Comparison with the mammalian enzyme."
    Kurosawa N., Kawasaki M., Hamamoto T., Nakaoka T., Lee Y.-C., Arita M., Tsuji S.
    Eur. J. Biochem. 219:375-381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Embryo.

Entry informationi

Entry nameiSIAT1_CHICK
AccessioniPrimary (citable) accession number: Q92182
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 17, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.