Reviewed, UniProtKB/Swiss-Prot Q92182 (SIAT1_CHICK)

Names and origin

Protein names

Recommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 1
    EC=2.4.99.1
Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
      Short name=Alpha 2,6-ST
      Short name=Sialyltransferase 1
    ST6Gal I

Gene names

Name:	ST6GAL1
Synonyms:	SIAT1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Tissue specificity	Highly expressed in testis, brain and liver and to a lesser extent in lung and heart.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactoside alpha-2,6-sialyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Beta-galactoside alpha-2,6-sialyltransferase 1		PRO_0000149252
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 26	17	Signal-anchor for type II membrane protein Potential
	Topological domain	27 – 413	387	Lumenal Potential
Amino acid modifications
	Glycosylation	129	1	N-linked (GlcNAc...) Potential
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Disulfide bond	190 ↔ 342		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92182-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 19B8CCD361ED137D	FASTA	413	47,393
	10 20 30 40 50 60 MVHINVLKKF MCVLVVILIA LTVCLWKETR GSYYVPLKND GTQVHRALDK WNLLKSQGLL 70 80 90 100 110 120 HEAAGEMGQM PKALPNNQNK VKGITSGAVE KSRKAAEHVK VWDKDSSSRN LIPRLQKVRK 130 140 150 160 170 180 NYLSMNKYNV TYNGKMNAAK LSPEQLLCRL RDRVNVTMIR GSDGPFNSSE WQHYLPDKSL 190 200 210 220 230 240 NETVGRLGRC AVVSSAGSLK SSHLGPEIDS HDAVLRFNGA PVKGFQEDVG QKTTIRLVNS 250 260 270 280 290 300 QLVTVEEQQF LKDALYNTGI LIVWDPAPYH AEIHEWYRKP DYKFFEAYKS YRIRHPEQPF 310 320 330 340 350 360 YILNPKMQWQ LWDILQENSL EHIQPNPPSS GMLGIVIMMT LCDEVDVYEF LPSKRQTDIC 370 380 390 400 410 HYYQKFHDHA CTMGAYHPLL FEKNLVKHLN QGTDEDIYTH GKVTLPGFRN VHC

References

[1]

"Molecular cloning and expression of chick embryo Gal beta 1,4GlcNAc alpha 2,6-sialyltransferase. Comparison with the mammalian enzyme." Kurosawa N., Kawasaki M., Hamamoto T., Nakaoka T., Lee Y.-C., Arita M., Tsuji S. Eur. J. Biochem. 219:375-381(1994) [PubMed: 8307003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75558 mRNA. Translation: CAA53235.1.
IPI	IPI00591409.
PIR	S41114.
RefSeq	NP_990572.1.
UniGene	Gga.1148
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q92182.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Genome annotation databases
Ensembl	ENSGALT00000008903; ENSGALP00000008889; ENSGALG00000005550; Gallus gallus. [Genome view]
GeneID	396169.
KEGG	gga:396169.
Organism-specific databases
CTD	396169.
Phylogenomic databases
HOGENOM	Q92182.
HOVERGEN	Q92182.
Enzyme and pathway databases
BRENDA	2.4.99.1. 4.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SIAT1_CHICK

Accession

Primary (citable) accession number: Q92182

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 22, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)