Reviewed, UniProtKB/Swiss-Prot Q92180 (GCNT1_BOVIN)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
    EC=2.4.1.102
Alternative name(s):
    Core 2-branching enzyme
    Core2-GlcNAc-transferase
      Short name=Core 2 GNT
      Short name=C2GNT

Gene names

Name:

GCNT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Forms critical branches in O-glycans.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase		PRO_0000191394
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 32	23	Signal-anchor for type II membrane protein Potential
	Topological domain	33 – 427	395	Lumenal Potential
	Region	33 – 121	89	Stem region By similarity
	Region	122 – 427	306	Catalytic By similarity
Sites
	Active site	320	1	By similarity
Amino acid modifications
	Modified residue	267	1	Phosphothreonine By similarity
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Disulfide bond	59 ↔ 412		By similarity
	Disulfide bond	100 ↔ 172		By similarity
	Disulfide bond	151 ↔ 199		By similarity
	Disulfide bond	372 ↔ 380		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92180-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 41B9CBFD948D4196	FASTA	427	49,734
	10 20 30 40 50 60 MLRKLWRRKL FSFPTKYYFL FLAFSVVTFT VLRIHQKTEF VNFGHLELFE ENPSSNINCT 70 80 90 100 110 120 KILQGDVDEI QKVKLESLTV KFKKRARWTN YDYINMTGDC ASFIKKRKYI TEPLSKEEAG 130 140 150 160 170 180 FPIAYSIVVH HKIEMLDRLL RAIYMPQNFY CIHVDAKSEK SFLAAAVGIA SCFSNVFVAS 190 200 210 220 230 240 QLESVVYASW SRVQADLNCM QDLYQMNAGW KYLINLCGMD FPIKTNLEIV RKLKLLMGEN 250 260 270 280 290 300 NLETEKMPSH KKERWKKHYE VVNGKLTNMG TDKIHPPLET PLFSGSAHFV VSREYVEYVL 310 320 330 340 350 360 QNQNIQKFME WAKDTYSPDE YLWATIQRIP EVPGSLSLSY KYDTSDMQAI ARFVKWQYFE 370 380 390 400 410 420 GDVSKGAPYP PCSVHVRSVC VFGAGDLNWL LHVHHLFANK FDTDIDLFAI QCLDEHLRHK ALETLKP

References

[1]	"Mucin biosynthesis: molecular cloning and expression of bovine lung mucin core 2 N-acetylglucosaminyltransferase cDNA." Li C.-M., Adler K.B., Cheng P.W. Am. J. Respir. Cell Mol. Biol. 18:343-352(1998) [PubMed: 9490652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U41320 mRNA. Translation: AAA83244.1.
IPI	IPI00686347.
RefSeq	NP_803476.1.
UniGene	Bt.91783
3D structure databases
SMR	Q92180. Positions 51-423.
ModBase	Search...
Protein family/group databases
CAZy	GT14. Glycosyltransferase Family 14.
Genome annotation databases
Ensembl	ENSBTAG00000012757. Bos taurus. [Contig view]
GeneID	281778.
KEGG	bta:281778.
Phylogenomic databases
HOVERGEN	Q92180.
Enzyme and pathway databases
BRENDA	2.4.1.102. 251.
Family and domain databases
InterPro	IPR003406. Glyco_trans_14. [Graphical view]
PANTHER	PTHR19297. Glyco_trans_14. 1 hit.
Pfam	PF02485. Branch. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCNT1_BOVIN

Accession

Primary (citable) accession number: Q92180

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 14, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)