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Q92179 (CHST3_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Carbohydrate sulfotransferase 3
EC=2.8.2.17
Alternative name(s):
Chondroitin 6-O-sulfotransferase 1
Short name=C6ST-1
Gene names
Name:CHST3
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices By similarity.

Catalytic activity

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentGolgi apparatus
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionTransferase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionchondroitin 6-sulfotransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 458458Carbohydrate sulfotransferase 3
PRO_0000085191

Regions

Topological domain1 – 2323Cytoplasmic Potential
Transmembrane24 – 3714Helical; Signal-anchor for type II membrane protein; Potential
Topological domain38 – 458421Lumenal Potential
Nucleotide binding121 – 1277PAPS By similarity
Nucleotide binding281 – 2899PAPS By similarity

Amino acid modifications

Glycosylation621N-linked (GlcNAc...) Potential
Glycosylation731N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation2361N-linked (GlcNAc...) Potential
Glycosylation3991N-linked (GlcNAc...) Potential
Glycosylation4431N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q92179 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C9A3B7D0A5086F0C

FASTA45852,253
        10         20         30         40         50         60 
MERRSALPQD FREVLHCLKM RSKYAVLLVF VVGLVIIEKE NNFISRVSDK LKQSPQVLPE 

        70         80         90        100        110        120 
ANETEASPVQ AENGSLASLR QLDTAFSQLR TRLRNVTLQL AGELGIAAPE PRRHVLLMAT 

       130        140        150        160        170        180 
TRTGSSFVGE FFNQQGNIFY LFEPLWHIER TVTFEPGGAN AVGSALVYRD VLQQLLLCDL 

       190        200        210        220        230        240 
YILESFISPA PEEHLTAALF RRGSSHSLCE EPVCTPSLKK VFEKYHCKNR RCGPLNITLA 

       250        260        270        280        290        300 
AEACRRKQHM ALKTVRIRQL EFLQPLAEDP RLDLRIIQLV RDPRAVLVSR MVAFSGKYES 

       310        320        330        340        350        360 
WKKWAAEGEA PLQEDEVQRL RGNCESIRLS AELGLRQPRW LRGRYMLVRY EDVARAPLRK 

       370        380        390        400        410        420 
ALEMYRFAGI HPTPQVEEWI RANTQAPQDS NGIYSTQKNS SEQFEKWRFS IPFKLAQVVQ 

       430        440        450 
DACEPAMRLF GYKLASSAQE LTNRSLSLLE EGPPTRIT

« Hide

References

[1]"Molecular cloning and expression of chick chondrocyte chondroitin 6-sulfotransferase."
Fukuta M., Uchimura K., Nakashima K., Kato M., Kimata K., Shinomura T., Habuchi O.
J. Biol. Chem. 270:18575-18580(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 38-44; 46-52 AND 186-192.
Strain: White leghorn.
Tissue: Embryonic chondrocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D49915 mRNA. Translation: BAA08655.1.
IPIIPI00598868.
PIRA57397.
RefSeqNP_990452.1. NM_205121.1.
UniGeneGga.599.

3D structure databases

ProteinModelPortalQ92179.
ModBaseSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000039793.

Proteomic databases

PaxDbQ92179.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396018.
KEGGgga:396018.

Organism-specific databases

CTD9469.

Phylogenomic databases

eggNOGNOG80862.
HOGENOMHOG000261614.
HOVERGENHBG106811.
InParanoidQ92179.
KOK01020.
OrthoDBEOG40P470.

Enzyme and pathway databases

BRENDA2.8.2.17. 1306.

Family and domain databases

InterProIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio20816080.

Entry information

Entry nameCHST3_CHICK
AccessionPrimary (citable) accession number: Q92179
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 29, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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