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Q92178

- CAN2_CHICK

UniProt

Q92178 - CAN2_CHICK

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Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 7 calcium ions.By similarity

Enzyme regulationi

Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi96 – 961Calcium 3By similarity
Active sitei105 – 1051By similarity
Metal bindingi175 – 1751Calcium 3By similarity
Metal bindingi229 – 2291Calcium 2By similarity
Metal bindingi230 – 2301Calcium 2By similarity
Active sitei262 – 2621By similarity
Active sitei286 – 2861By similarity
Metal bindingi292 – 2921Calcium 4By similarity
Metal bindingi299 – 2991Calcium 4By similarity
Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi545 – 5451Calcium 5By similarity
Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
Metal bindingi552 – 5521Calcium 5By similarity
Metal bindingi585 – 5851Calcium 6By similarity
Metal bindingi587 – 5871Calcium 6By similarity
Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
Metal bindingi596 – 5961Calcium 6By similarity
Metal bindingi615 – 6151Calcium 7By similarity
Metal bindingi617 – 6171Calcium 7By similarity
Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
Metal bindingi626 – 6261Calcium 7By similarity
Metal bindingi658 – 6581Calcium 1By similarity
Metal bindingi661 – 6611Calcium 1By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi585 – 596121Add
BLAST
Calcium bindingi615 – 626122Add
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. cellular response to amino acid stimulus Source: UniProtKB
  2. proteolysis Source: UniProtKB
  3. regulation of cytoskeleton organization Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.53. 1306.

Protein family/group databases

MEROPSiC02.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-2 catalytic subunit (EC:3.4.22.53)
Alternative name(s):
Calcium-activated neutral proteinase 2
Short name:
CANP 2
Calpain M-type
Calpain-2 large subunit
Millimolar-calpain
Short name:
M-calpain
Gene namesi
Name:CAPN2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. dendrite Source: UniProtKB
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000026495Add
BLAST
Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000026496Add
BLAST

Proteomic databases

PaxDbiQ92178.

Expressioni

Tissue specificityi

Ubiquitous.

Interactioni

Subunit structurei

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Structurei

3D structure databases

ProteinModelPortaliQ92178.
SMRiQ92178. Positions 2-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini667 – 70034EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni345 – 514170Domain IIIAdd
BLAST
Regioni515 – 52915LinkerAdd
BLAST
Regioni530 – 700171Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ92178.
KOiK03853.
PhylomeDBiQ92178.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92178 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGMAAALAK ERAAAAGAGR HGQAVPYLGQ DFGALRRECL QGGRLFHDPS
60 70 80 90 100
FPAGPAALGY RELGPNSYKT KGVVWCRPTE LCSCPRFIAG GATRTDICQG
110 120 130 140 150
ALGDCWLLAA IASLTLNEEI LARVVPRDQS FQDEYAGIFH FQFWQYGEWV
160 170 180 190 200
DVVVDDRLPT KNGELLFVHS AEGSEFWSAL LEKAYAKLNG SYEALSGGTT
210 220 230 240 250
TEGFEDFTGG IAEWYELQKA PPNLFKIIQK ALQKGSLLGC SIDITSAAET
260 270 280 290 300
EAVTSQKLVK GHAYSVTGAE EVNFRGSIQK LIRIRNPWGE VEWTGKWNDN
310 320 330 340 350
CPNWSGVDPE VRERLTRRHE DGEFWMAFND FLRHYSRLEI CNLTPDTLAS
360 370 380 390 400
DRYKKWSLLK LDGNWRRGAT AGGCRNYPNT FWTNPQYLIK LEEEDEDPDD
410 420 430 440 450
PEGGCTFLIG LIQKHRRKQR KMGEDMHTIG FAIYEVPPEF SGQTNIHLSK
460 470 480 490 500
NFFLTNKARE KSNTFINLRE VLNRFKLPAG EYIIVPSTFE PNLNGDFCLR
510 520 530 540 550
VFSEKNANST VIDDEIEANF EETEIDEDDI EPSFKKLFGQ LAGSDAEISA
560 570 580 590 600
FELRSILNKI LAKRQDIKSD GFSIETCKIM VDLLDNDGSG KLGLKEFHTL
610 620 630 640 650
WTKIQKYQKI YREIDVDRSG TMNSYEMRRA LEAAGFKLSC QLHQIIVARF
660 670 680 690 700
ADEDLIIDFD NCVRCLIRLE TLYKMFRKLD TEKTGTIELN LINWLFFTVI
Length:700
Mass (Da):79,229
Last modified:January 23, 2007 - v3
Checksum:iC3AEDB39CCB56D3B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38026 mRNA. Translation: BAA07228.1.
PIRiS57194.
RefSeqiNP_990411.1. NM_205080.1.
UniGeneiGga.3677.

Genome annotation databases

GeneIDi395963.
KEGGigga:395963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D38026 mRNA. Translation: BAA07228.1 .
PIRi S57194.
RefSeqi NP_990411.1. NM_205080.1.
UniGenei Gga.3677.

3D structure databases

ProteinModelPortali Q92178.
SMRi Q92178. Positions 2-700.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q92178.

Protein family/group databases

MEROPSi C02.002.

Proteomic databases

PaxDbi Q92178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 395963.
KEGGi gga:395963.

Organism-specific databases

CTDi 824.

Phylogenomic databases

eggNOGi NOG327523.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi Q92178.
KOi K03853.
PhylomeDBi Q92178.

Enzyme and pathway databases

BRENDAi 3.4.22.53. 1306.

Miscellaneous databases

NextBioi 20816028.
PROi Q92178.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029539. CAPN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a third ubiquitous calpain species -- chicken muscle expresses four distinct calpains."
    Sorimachi H., Tsukahara T., Okada-Ban M., Sugita H., Ishiura S., Suzuki K.
    Biochim. Biophys. Acta 1261:381-393(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Muscle.

Entry informationi

Entry nameiCAN2_CHICK
AccessioniPrimary (citable) accession number: Q92178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3