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Q92178

- CAN2_CHICK

UniProt

Q92178 - CAN2_CHICK

Protein

Calpain-2 catalytic subunit

Gene

CAPN2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 7 calcium ions.By similarity

    Enzyme regulationi

    Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi91 – 911Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi96 – 961Calcium 3By similarity
    Active sitei105 – 1051By similarity
    Metal bindingi175 – 1751Calcium 3By similarity
    Metal bindingi229 – 2291Calcium 2By similarity
    Metal bindingi230 – 2301Calcium 2By similarity
    Active sitei262 – 2621By similarity
    Active sitei286 – 2861By similarity
    Metal bindingi292 – 2921Calcium 4By similarity
    Metal bindingi299 – 2991Calcium 4By similarity
    Metal bindingi323 – 3231Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi542 – 5421Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi545 – 5451Calcium 5By similarity
    Metal bindingi547 – 5471Calcium 5; via carbonyl oxygenBy similarity
    Metal bindingi552 – 5521Calcium 5By similarity
    Metal bindingi585 – 5851Calcium 6By similarity
    Metal bindingi587 – 5871Calcium 6By similarity
    Metal bindingi589 – 5891Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi591 – 5911Calcium 6; via carbonyl oxygenBy similarity
    Metal bindingi596 – 5961Calcium 6By similarity
    Metal bindingi615 – 6151Calcium 7By similarity
    Metal bindingi617 – 6171Calcium 7By similarity
    Metal bindingi619 – 6191Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi621 – 6211Calcium 7; via carbonyl oxygenBy similarity
    Metal bindingi626 – 6261Calcium 7By similarity
    Metal bindingi658 – 6581Calcium 1By similarity
    Metal bindingi661 – 6611Calcium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi585 – 596121Add
    BLAST
    Calcium bindingi615 – 626122Add
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to amino acid stimulus Source: UniProtKB
    2. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.4.22.53. 1306.

    Protein family/group databases

    MEROPSiC02.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-2 catalytic subunit (EC:3.4.22.53)
    Alternative name(s):
    Calcium-activated neutral proteinase 2
    Short name:
    CANP 2
    Calpain M-type
    Calpain-2 large subunit
    Millimolar-calpain
    Short name:
    M-calpain
    Gene namesi
    Name:CAPN2
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Translocates to the plasma membrane upon Ca2+ binding.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. dendrite Source: UniProtKB
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Propeptidei2 – 1918Anchors to the small subunitSequence AnalysisPRO_0000026495Add
    BLAST
    Chaini20 – 700681Calpain-2 catalytic subunitPRO_0000026496Add
    BLAST

    Proteomic databases

    PaxDbiQ92178.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Interactioni

    Subunit structurei

    Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

    Structurei

    3D structure databases

    ProteinModelPortaliQ92178.
    SMRiQ92178. Positions 2-700.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini572 – 60534EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini602 – 63736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini667 – 70034EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni345 – 514170Domain IIIAdd
    BLAST
    Regioni515 – 52915LinkerAdd
    BLAST
    Regioni530 – 700171Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiQ92178.
    KOiK03853.
    PhylomeDBiQ92178.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PANTHERiPTHR10183:SF268. PTHR10183:SF268. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q92178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGMAAALAK ERAAAAGAGR HGQAVPYLGQ DFGALRRECL QGGRLFHDPS    50
    FPAGPAALGY RELGPNSYKT KGVVWCRPTE LCSCPRFIAG GATRTDICQG 100
    ALGDCWLLAA IASLTLNEEI LARVVPRDQS FQDEYAGIFH FQFWQYGEWV 150
    DVVVDDRLPT KNGELLFVHS AEGSEFWSAL LEKAYAKLNG SYEALSGGTT 200
    TEGFEDFTGG IAEWYELQKA PPNLFKIIQK ALQKGSLLGC SIDITSAAET 250
    EAVTSQKLVK GHAYSVTGAE EVNFRGSIQK LIRIRNPWGE VEWTGKWNDN 300
    CPNWSGVDPE VRERLTRRHE DGEFWMAFND FLRHYSRLEI CNLTPDTLAS 350
    DRYKKWSLLK LDGNWRRGAT AGGCRNYPNT FWTNPQYLIK LEEEDEDPDD 400
    PEGGCTFLIG LIQKHRRKQR KMGEDMHTIG FAIYEVPPEF SGQTNIHLSK 450
    NFFLTNKARE KSNTFINLRE VLNRFKLPAG EYIIVPSTFE PNLNGDFCLR 500
    VFSEKNANST VIDDEIEANF EETEIDEDDI EPSFKKLFGQ LAGSDAEISA 550
    FELRSILNKI LAKRQDIKSD GFSIETCKIM VDLLDNDGSG KLGLKEFHTL 600
    WTKIQKYQKI YREIDVDRSG TMNSYEMRRA LEAAGFKLSC QLHQIIVARF 650
    ADEDLIIDFD NCVRCLIRLE TLYKMFRKLD TEKTGTIELN LINWLFFTVI 700
    Length:700
    Mass (Da):79,229
    Last modified:January 23, 2007 - v3
    Checksum:iC3AEDB39CCB56D3B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38026 mRNA. Translation: BAA07228.1.
    PIRiS57194.
    RefSeqiNP_990411.1. NM_205080.1.
    UniGeneiGga.3677.

    Genome annotation databases

    GeneIDi395963.
    KEGGigga:395963.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38026 mRNA. Translation: BAA07228.1 .
    PIRi S57194.
    RefSeqi NP_990411.1. NM_205080.1.
    UniGenei Gga.3677.

    3D structure databases

    ProteinModelPortali Q92178.
    SMRi Q92178. Positions 2-700.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q92178.

    Protein family/group databases

    MEROPSi C02.002.

    Proteomic databases

    PaxDbi Q92178.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395963.
    KEGGi gga:395963.

    Organism-specific databases

    CTDi 824.

    Phylogenomic databases

    eggNOGi NOG327523.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi Q92178.
    KOi K03853.
    PhylomeDBi Q92178.

    Enzyme and pathway databases

    BRENDAi 3.4.22.53. 1306.

    Miscellaneous databases

    NextBioi 20816028.
    PROi Q92178.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029539. CAPN2.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    PANTHERi PTHR10183:SF268. PTHR10183:SF268. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a third ubiquitous calpain species -- chicken muscle expresses four distinct calpains."
      Sorimachi H., Tsukahara T., Okada-Ban M., Sugita H., Ishiura S., Suzuki K.
      Biochim. Biophys. Acta 1261:381-393(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Muscle.

    Entry informationi

    Entry nameiCAN2_CHICK
    AccessioniPrimary (citable) accession number: Q92178
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3