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Protein

Calpain-3

Gene

CAPN3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.

Catalytic activityi

Broad endopeptidase activity.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei123 – 1231By similarity
Active sitei327 – 3271By similarity
Active sitei351 – 3511By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi694 – 705121CuratedAdd
BLAST
Calcium bindingi724 – 735122CuratedAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.54. 1306.

Protein family/group databases

MEROPSiC02.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-3 (EC:3.4.22.54)
Alternative name(s):
Calcium-activated neutral proteinase 3
Short name:
CANP 3
Calpain L3
Calpain p94
Muscle-specific calcium-activated neutral protease 3
Gene namesi
Name:CAPN3
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810Calpain-3PRO_0000207712Add
BLAST

Proteomic databases

PaxDbiQ92177.
PRIDEiQ92177.

Expressioni

Tissue specificityi

Skeletal muscle. Low levels in spleen, intestine and bone.

Interactioni

Subunit structurei

Heterodimer of a large (catalytic) and a small (regulatory) subunit.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000031999.

Structurei

3D structure databases

ProteinModelPortaliQ92177.
SMRiQ92177. Positions 54-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 410343Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini638 – 67235EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini681 – 71434EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini711 – 74636EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini776 – 81035EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni411 – 579169Domain IIIAdd
BLAST
Regioni580 – 63859LinkerAdd
BLAST
Regioni639 – 809171Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ92177.
PhylomeDBiQ92177.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSAINAAVA QQTAAGSVPS TTSTTTEGTG GGTGGIYSAI ISRNQPIIKV
60 70 80 90 100
KEKTYEELHK KCLEENILYE DPDFPPNETS LFYSQKVPIK FEWKRPREIC
110 120 130 140 150
ENPRFIIGGA NRTDICQGEL GDCWFLAAIA CLTLNKKLLC RVIPHDQSFI
160 170 180 190 200
QNYAGIFHFQ FWRYGDWVDV IIDDCLPTYN NQLVFTKSSQ RNEFWSALLE
210 220 230 240 250
KAYAKLHGSY EALKGGNTTE AMEDFTGGVI EFYEIKDAPK DIYKIMKHAI
260 270 280 290 300
ARGSLMASSI DDNLGFHYGA APRSDIGELI ARMVKNLENA QMTYSTVDYQ
310 320 330 340 350
GTDERPAWTI MPMQYETRMS CGLVKGHAYS VTAVEETTYK GEKMRLVRLR
360 370 380 390 400
NPWGQVEWNG PWSDKSEEWN FIDEEEKIRL QHKIAEDGEF WISLEDFMRH
410 420 430 440 450
FTKLEICNLT PDTLEADKLQ TWTVSVNEGR WVRGCSAGGC RNYPDTFWTN
460 470 480 490 500
PQYRLKLLEE DDDPEDEEVI CSFLVALMQK NRRKERKLGA NLYTIGFAIY
510 520 530 540 550
EVPKEMHGTK HHLQKDFFLY NASKARSKTY INMREISERF RLPPSEYVII
560 570 580 590 600
PSTYEPHQEG EFILRVFSEK RSLSEEVENM IEADRPSKKK KGKPIIFVSD
610 620 630 640 650
RANSNKELTT DEDAGKDGEK THVDEKKRSS AKAREKSEEE TQFRNIFRQI
660 670 680 690 700
AGDDMEICRE ELRNVLNNVV KKHKDLKTEG FELESSRSMI ALMDTDGSGK
710 720 730 740 750
INFDEFRHLW DKIKSWQKIF KHYDADHSGT INSYEMRNAV KDAGFRLNNQ
760 770 780 790 800
LYDIITMRYA DKNMNIDFDS FICCFVRLDA MFRAFHAFDK DGDGIIKLNV
810
LEWLQLTMYA
Length:810
Mass (Da):93,561
Last modified:November 1, 1997 - v1
Checksum:iE8DE99411C51041E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38028 mRNA. Translation: BAA07230.1.
PIRiS57196.
UniGeneiGga.72.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38028 mRNA. Translation: BAA07230.1.
PIRiS57196.
UniGeneiGga.72.

3D structure databases

ProteinModelPortaliQ92177.
SMRiQ92177. Positions 54-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000031999.

Protein family/group databases

MEROPSiC02.004.

Proteomic databases

PaxDbiQ92177.
PRIDEiQ92177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ92177.
PhylomeDBiQ92177.

Enzyme and pathway databases

BRENDAi3.4.22.54. 1306.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN3_CHICK
AccessioniPrimary (citable) accession number: Q92177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.