Reviewed, UniProtKB/Swiss-Prot Q92142 (TPH_XENLA)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase

Gene names

Name:

tph

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Subunit structure	Multimer of identical subunits By similarity.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW serotonin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Tryptophan 5-hydroxylase		PRO_0000205573
Regions
	Domain	55 – 129	75	ACT
Sites
	Metal binding	309	1	Iron By similarity
	Metal binding	314	1	Iron By similarity
	Metal binding	354	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92142-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6852C33EFF8DEBA0	FASTA	481	55,406
	10 20 30 40 50 60 MYSNRKECPR RVKSFDSVNS GLDENQINNE FNKSTYIKIE DNKEYSENVC ERGKASVIFS 70 80 90 100 110 120 LKNEIGGLVK ALKLFQEKHV NLIHIESRKS KRRNSEFEIF VDCDSNREQL NEIFQLLKPH 130 140 150 160 170 180 VNVISMSPPE NFTVQEDDME SVPWFPKKIS DLDKCANRVL MYGSDLDADH PGFKDNVYRK 190 200 210 220 230 240 RRKYFADVAM SYKYGDPIPH IEFTEEEIQT WGTVFRELNK LYPTHACREY LKNLPLLSKH 250 260 270 280 290 300 CGYREDNIPQ LEDVSRFLRE RTGFTIRPVA GYLSPRDFLA GLAFRVFHCT QYVRHDSDPL 310 320 330 340 350 360 NTPEPDTCHE LLGHVPLLAE PSFAQFSQEI GLASLGASDE AVQKLATCYF FTVEFGLCKQ 370 380 390 400 410 420 EGKLKVYGAG LLSSISELKH SLSGNAKVKP FDPMVTCNQE CIITSFQELY FVSESFEEAK 430 440 450 460 470 480 EKMREFAKTI QRPFGLKYNP FTQSVDILKD TKSIAMVVRE LRHELDIVND ALNKMNKQLG V

References

[1]	"Tryptophan hydroxylase expression is regulated by a circadian clock in Xenopus laevis retina." Green C.B., Besharse J.C. J. Neurochem. 62:2420-2428(1994) [PubMed: 8189245] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L20679 mRNA. Translation: AAA21306.1.
PIR	I51567.
RefSeq	NP_001080923.1.
UniGene	Xl.1144
3D structure databases
HSSP	HSSP built from PDB template 1MLW based on UniProtKB P17752.
SMR	Q92142. Positions 141-430.
ModBase	Search...
Genome annotation databases
GeneID	387560.
KEGG	xla:387560.
Organism-specific databases
CTD	387560.
Xenbase	XB-FEAT-996925. tph1.
Phylogenomic databases
HOVERGEN	Q92142.
Enzyme and pathway databases
BRENDA	1.14.16.4. 648.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPH_XENLA

Accession

Primary (citable) accession number: Q92142

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project