Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sodium/potassium-transporting ATPase subunit alpha-1

Gene

atp1a1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3784-aspartylphosphate intermediateBy similarity1
Binding sitei489ATPBy similarity1
Metal bindingi719MagnesiumBy similarity1
Metal bindingi723MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • sodium:potassium-exchanging ATPase activity Source: BHF-UCL

GO - Biological processi

  • cellular potassium ion homeostasis Source: BHF-UCL
  • cellular sodium ion homeostasis Source: BHF-UCL
  • membrane repolarization Source: BHF-UCL
  • potassium ion import Source: BHF-UCL
  • sodium ion export from cell Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-1 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-1 subunit
Alternative name(s):
Sodium pump subunit alpha-1
Gene namesi
Name:atp1a1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254180. atp1a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 89CytoplasmicSequence analysisAdd BLAST89
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Topological domaini111 – 133ExtracellularSequence analysisAdd BLAST23
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Topological domaini155 – 290CytoplasmicSequence analysisAdd BLAST136
Transmembranei291 – 310HelicalSequence analysisAdd BLAST20
Topological domaini311 – 322ExtracellularSequence analysisAdd BLAST12
Transmembranei323 – 340HelicalSequence analysisAdd BLAST18
Topological domaini341 – 774CytoplasmicSequence analysisAdd BLAST434
Transmembranei775 – 794HelicalSequence analysisAdd BLAST20
Topological domaini795 – 804ExtracellularSequence analysis10
Transmembranei805 – 825HelicalSequence analysisAdd BLAST21
Topological domaini826 – 845CytoplasmicSequence analysisAdd BLAST20
Transmembranei846 – 868HelicalSequence analysisAdd BLAST23
Topological domaini869 – 920ExtracellularSequence analysisAdd BLAST52
Transmembranei921 – 940HelicalSequence analysisAdd BLAST20
Topological domaini941 – 953CytoplasmicSequence analysisAdd BLAST13
Transmembranei954 – 972HelicalSequence analysisAdd BLAST19
Topological domaini973 – 987ExtracellularSequence analysisAdd BLAST15
Transmembranei988 – 1008HelicalSequence analysisAdd BLAST21
Topological domaini1009 – 1025CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • sodium:potassium-exchanging ATPase complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462961 – 1025Sodium/potassium-transporting ATPase subunit alpha-1Add BLAST1025

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Phosphoserine; by PKCBy similarity1
Modified residuei945Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ92123.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Structurei

3D structure databases

ProteinModelPortaliQ92123.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 86Interaction with phosphoinositide-3 kinaseBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi21 – 28Poly-Lys8

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004298.
KOiK01539.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92123-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYGAGRDKY EPAATSEQGG KKKKGKGKGK EKDMDELKKE VTMEDHKLSL
60 70 80 90 100
DELHRKFGTD MQRGLTTARA AEILARDGPN ALTPPPTTPE WVKFCRQLFG
110 120 130 140 150
GFSMLLWIGA ILCFLAYGIQ AAMEEEPQND NLYLGVVLSA VVIITGCFSY
160 170 180 190 200
YQEAKSSKIM ESFKNMVPQQ ALVIRSGEKL SINAEEVVLG DLVEVKGGDR
210 220 230 240 250
IPADLRVISS HGCKVDNSSL TGESEPQTRS PDFTNENPLE TRNIAFFSTN
260 270 280 290 300
CVEGTARGIV VNTGDRTVMG RIATLASGLD GGRTPIAIEI EHFIHIITGV
310 320 330 340 350
AVFLGVSFFI LSLILQYTWL EAVIFLIGII VANVPEGLLA TVTVCLTLTA
360 370 380 390 400
KRMARKNCLV KNLEAVETLG STSTICSDKT GTLTQNRMTV AHMWFDNQIH
410 420 430 440 450
EADTTENQSG ASFDKSSPTW TALSRVAGLC NRAVFQAGQE NTPILKRDVA
460 470 480 490 500
GDASESALLK CIELCCGSVR DMREKNHKVA EIPFNSTNKY QLSVHKNANP
510 520 530 540 550
SESRYILVMK GAPERILDRC TSIILQGKEQ PLDEELKDAF QNAYLELGGL
560 570 580 590 600
GERVLGFCHL ALPDDQFPDG FQFDTEEVNF PTENLCFVGL ISMIDPPRAA
610 620 630 640 650
VPDAVGKCRS AGIKVIMVTG DHPITAKAIA KGVGIISEGN ETVEDIAARL
660 670 680 690 700
NIPVNQVNPR DAKACVIHGT DLKDMTEEQI DDILRHHTEI VFARTSPQQK
710 720 730 740 750
LIIVEGCQRQ GAIVAVTGDG VNDSPALKKA DIGIAMGIAG SDVSKQAADM
760 770 780 790 800
ILLDDNFASI VTGVEEGRLI FDNLKKSIAY TLTSNIPEIT PFLIFIIANI
810 820 830 840 850
PLPLGTVTIL CIDLGTDMVP AISLAYEQAE SDIMKRQPRN PKTDKLVNER
860 870 880 890 900
LISMAYGQIG MIQALGGFFT YFVILAENGF LPWTLLGIRV NWDDRWTNDV
910 920 930 940 950
EDSYGQQWTY EQRKIVEFTC HTSFFISIVV VQWADLIICK TRRNSVFQQG
960 970 980 990 1000
MKNKILIFGL FEETALAAFL SYCPGMDVAL RMYPLKPTWW FCAFPYSLII
1010 1020
FIYDEVRKLI IRRSPGGWVE KESYY
Length:1,025
Mass (Da):113,084
Last modified:November 1, 1996 - v1
Checksum:iB5F9B4EB6D86EEA0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9K → Q in AAC59760 (PubMed:8631513).Curated1
Sequence conflicti29 – 30Missing in AAC59759 (PubMed:8631513).Curated2
Sequence conflicti29 – 30Missing in AAC59760 (PubMed:8631513).Curated2
Sequence conflicti31E → D in AAC59760 (PubMed:8631513).Curated1
Sequence conflicti61 – 63MQR → LQK in AAC59760 (PubMed:8631513).Curated3
Sequence conflicti67T → S in AAC59760 (PubMed:8631513).Curated1
Sequence conflicti81A → S in AAC59760 (PubMed:8631513).Curated1
Sequence conflicti92V → G in AAC59760 (PubMed:8631513).Curated1
Sequence conflicti102F → L in AAC59760 (PubMed:8631513).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10108 mRNA. Translation: AAA19022.1.
U49238 mRNA. Translation: AAC59759.1.
U49239 mRNA. Translation: AAC59760.1.
PIRiA60444.
RefSeqiNP_001084064.1. NM_001090595.1.
UniGeneiXl.3792.

Genome annotation databases

GeneIDi399285.
KEGGixla:399285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10108 mRNA. Translation: AAA19022.1.
U49238 mRNA. Translation: AAC59759.1.
U49239 mRNA. Translation: AAC59760.1.
PIRiA60444.
RefSeqiNP_001084064.1. NM_001090595.1.
UniGeneiXl.3792.

3D structure databases

ProteinModelPortaliQ92123.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ92123.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399285.
KEGGixla:399285.

Organism-specific databases

CTDi399285.
XenbaseiXB-GENE-6254180. atp1a1.

Phylogenomic databases

HOVERGENiHBG004298.
KOiK01539.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT1A1_XENLA
AccessioniPrimary (citable) accession number: Q92123
Secondary accession number(s): Q91683, Q92127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.