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Q92122 (KPYM_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase PKM

EC=2.7.1.40
Alternative name(s):
Cytosolic thyroid hormone-binding protein
Short name=CTHBP
Gene names
Name:pkm
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May modulate the metamorphic process by regulating the level of intracellular thyroid hormones.

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Developmental stage

Expressed in all stages of tadpole development. In the tail, high levels found in the premetamorphic stage (levels 54-60). Levels decrease with tail resorption. In the hindlimb, low expression during limb morphogenesis (stages 54-56), increased levels with limb growth (stages 58-66). Levels increase only slightly during intestine remodeling.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Pyruvate kinase PKM
PRO_0000112098

Sites

Metal binding711Potassium By similarity
Metal binding731Potassium By similarity
Metal binding1091Potassium By similarity
Metal binding1101Potassium; via carbonyl oxygen By similarity
Metal binding2681Magnesium Potential
Metal binding2921Magnesium By similarity
Binding site691Substrate By similarity
Binding site2911Substrate; via amide nitrogen By similarity
Binding site2921Substrate; via amide nitrogen By similarity
Binding site3241Substrate By similarity
Site2661Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92122 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A8379F0E65325A66

FASTA52757,525
        10         20         30         40         50         60 
MSEAGSAFIQ TQQLHAAMAD TFLEHMCRLD IDSEPIVARN TGIICTIGPA SRSVEMLKEM 

        70         80         90        100        110        120 
IKSGMNIARL NFSHGTHEYH AGTIKNVREA TESLASNPIH YRPVAVALDT KGPEIRTGLI 

       130        140        150        160        170        180 
KGSGTAEVEL KKGATMRITL DDAFQENCDE NVLWVDYKNL TKVVKPGSKI YVDDGLISLL 

       190        200        210        220        230        240 
VKEIGPDFCV TEIENGGMLG SKKGVNLPGA AVDLPAVSSK DIQDLQFGVE QDVDMVFASF 

       250        260        270        280        290        300 
IRKAADVHEV REVLGEKGKN IKIISKIENH EGVRRFDEIL EASDGIMVAR GDLGIEIPAE 

       310        320        330        340        350        360 
KVFLAQKMMI GRCNRAGKPV ICATQMLESM IKKPRPTRAE GSDVANAVLD GADCIMLSGE 

       370        380        390        400        410        420 
TAKGDYPLEA VRMQHAIARE AEAAIFHRQL FEELRRVSPL TRDPTEATAV GAVEASFKCS 

       430        440        450        460        470        480 
SGAIIVLTKS GRSAHLLSRY RPRAPIISVT RNGQTARQAH LYRGIFPVLY REAVHEAWAE 

       490        500        510        520 
DVDSRVNFAM DIGKARGFFK SGDVVIVLTG WRPGSGFTNT MRVVPVP 

« Hide

References

« Hide 'large scale' references
[1]"Tissue-dependent developmental expression of a cytosolic thyroid hormone protein gene in Xenopus: its role in the regulation of amphibian metamorphosis."
Shi Y.-B., Liang V.C.-T., Parkison C., Cheng S.-Y.
FEBS Lett. 355:61-64(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Intestine.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U03878 mRNA. Translation: AAA63581.1.
BC079921 mRNA. Translation: AAH79921.1.
RefSeqNP_001084341.1. NM_001090872.1.
UniGeneXl.21936.

3D structure databases

ProteinModelPortalQ92122.
SMRQ92122. Positions 9-527.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid100773. 1 interaction.

Proteomic databases

PRIDEQ92122.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399448.
KEGGxla:399448.

Organism-specific databases

CTD5315.
XenbaseXB-GENE-485877. pkm.

Phylogenomic databases

HOVERGENHBG000941.
KOK00873.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYM_XENLA
AccessionPrimary (citable) accession number: Q92122
Secondary accession number(s): Q68FC1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways