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Protein

Pyruvate kinase PKM

Gene

pkm

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May modulate the metamorphic process by regulating the level of intracellular thyroid hormones.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathway: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapdh), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (gapdh)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Alpha-enolase (eno1)
  5. Pyruvate kinase, Pyruvate kinase (pklr), Pyruvate kinase PKM (pkm), Pyruvate kinase (LOC100037030)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691SubstrateBy similarity
Metal bindingi71 – 711PotassiumBy similarity
Metal bindingi73 – 731PotassiumBy similarity
Metal bindingi109 – 1091PotassiumBy similarity
Metal bindingi110 – 1101Potassium; via carbonyl oxygenBy similarity
Sitei266 – 2661Transition state stabilizerBy similarity
Metal bindingi268 – 2681MagnesiumSequence Analysis
Binding sitei291 – 2911Substrate; via amide nitrogenBy similarity
Metal bindingi292 – 2921MagnesiumBy similarity
Binding sitei292 – 2921Substrate; via amide nitrogenBy similarity
Binding sitei324 – 3241SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase PKM (EC:2.7.1.40)
Alternative name(s):
Cytosolic thyroid hormone-binding protein
Short name:
CTHBP
Gene namesi
Name:pkm
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-485877. pkm.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Pyruvate kinase PKMPRO_0000112098Add
BLAST

Proteomic databases

PRIDEiQ92122.

Expressioni

Developmental stagei

Expressed in all stages of tadpole development. In the tail, high levels found in the premetamorphic stage (levels 54-60). Levels decrease with tail resorption. In the hindlimb, low expression during limb morphogenesis (stages 54-56), increased levels with limb growth (stages 58-66). Levels increase only slightly during intestine remodeling.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi100773. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ92122.
SMRiQ92122. Positions 9-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

HOVERGENiHBG000941.
KOiK00873.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAGSAFIQ TQQLHAAMAD TFLEHMCRLD IDSEPIVARN TGIICTIGPA
60 70 80 90 100
SRSVEMLKEM IKSGMNIARL NFSHGTHEYH AGTIKNVREA TESLASNPIH
110 120 130 140 150
YRPVAVALDT KGPEIRTGLI KGSGTAEVEL KKGATMRITL DDAFQENCDE
160 170 180 190 200
NVLWVDYKNL TKVVKPGSKI YVDDGLISLL VKEIGPDFCV TEIENGGMLG
210 220 230 240 250
SKKGVNLPGA AVDLPAVSSK DIQDLQFGVE QDVDMVFASF IRKAADVHEV
260 270 280 290 300
REVLGEKGKN IKIISKIENH EGVRRFDEIL EASDGIMVAR GDLGIEIPAE
310 320 330 340 350
KVFLAQKMMI GRCNRAGKPV ICATQMLESM IKKPRPTRAE GSDVANAVLD
360 370 380 390 400
GADCIMLSGE TAKGDYPLEA VRMQHAIARE AEAAIFHRQL FEELRRVSPL
410 420 430 440 450
TRDPTEATAV GAVEASFKCS SGAIIVLTKS GRSAHLLSRY RPRAPIISVT
460 470 480 490 500
RNGQTARQAH LYRGIFPVLY REAVHEAWAE DVDSRVNFAM DIGKARGFFK
510 520
SGDVVIVLTG WRPGSGFTNT MRVVPVP
Length:527
Mass (Da):57,525
Last modified:November 1, 1996 - v1
Checksum:iA8379F0E65325A66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03878 mRNA. Translation: AAA63581.1.
BC079921 mRNA. Translation: AAH79921.1.
RefSeqiNP_001084341.1. NM_001090872.1.
UniGeneiXl.21936.

Genome annotation databases

GeneIDi399448.
KEGGixla:399448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03878 mRNA. Translation: AAA63581.1.
BC079921 mRNA. Translation: AAH79921.1.
RefSeqiNP_001084341.1. NM_001090872.1.
UniGeneiXl.21936.

3D structure databases

ProteinModelPortaliQ92122.
SMRiQ92122. Positions 9-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi100773. 1 interaction.

Proteomic databases

PRIDEiQ92122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399448.
KEGGixla:399448.

Organism-specific databases

CTDi5315.
XenbaseiXB-GENE-485877. pkm.

Phylogenomic databases

HOVERGENiHBG000941.
KOiK00873.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tissue-dependent developmental expression of a cytosolic thyroid hormone protein gene in Xenopus: its role in the regulation of amphibian metamorphosis."
    Shi Y.-B., Liang V.C.-T., Parkison C., Cheng S.-Y.
    FEBS Lett. 355:61-64(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Intestine.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiKPYM_XENLA
AccessioniPrimary (citable) accession number: Q92122
Secondary accession number(s): Q68FC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.