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Q92122

- KPYM_XENLA

UniProt

Q92122 - KPYM_XENLA

Protein

Pyruvate kinase PKM

Gene

pkm

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    May modulate the metamorphic process by regulating the level of intracellular thyroid hormones.

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691SubstrateBy similarity
    Metal bindingi71 – 711PotassiumBy similarity
    Metal bindingi73 – 731PotassiumBy similarity
    Metal bindingi109 – 1091PotassiumBy similarity
    Metal bindingi110 – 1101Potassium; via carbonyl oxygenBy similarity
    Sitei266 – 2661Transition state stabilizerBy similarity
    Metal bindingi268 – 2681MagnesiumSequence Analysis
    Binding sitei291 – 2911Substrate; via amide nitrogenBy similarity
    Metal bindingi292 – 2921MagnesiumBy similarity
    Binding sitei292 – 2921Substrate; via amide nitrogenBy similarity
    Binding sitei324 – 3241SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase PKM (EC:2.7.1.40)
    Alternative name(s):
    Cytosolic thyroid hormone-binding protein
    Short name:
    CTHBP
    Gene namesi
    Name:pkm
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-485877. pkm.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 527527Pyruvate kinase PKMPRO_0000112098Add
    BLAST

    Proteomic databases

    PRIDEiQ92122.

    Expressioni

    Developmental stagei

    Expressed in all stages of tadpole development. In the tail, high levels found in the premetamorphic stage (levels 54-60). Levels decrease with tail resorption. In the hindlimb, low expression during limb morphogenesis (stages 54-56), increased levels with limb growth (stages 58-66). Levels increase only slightly during intestine remodeling.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi100773. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92122.
    SMRiQ92122. Positions 9-527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    HOVERGENiHBG000941.
    KOiK00873.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92122-1 [UniParc]FASTAAdd to Basket

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    MSEAGSAFIQ TQQLHAAMAD TFLEHMCRLD IDSEPIVARN TGIICTIGPA    50
    SRSVEMLKEM IKSGMNIARL NFSHGTHEYH AGTIKNVREA TESLASNPIH 100
    YRPVAVALDT KGPEIRTGLI KGSGTAEVEL KKGATMRITL DDAFQENCDE 150
    NVLWVDYKNL TKVVKPGSKI YVDDGLISLL VKEIGPDFCV TEIENGGMLG 200
    SKKGVNLPGA AVDLPAVSSK DIQDLQFGVE QDVDMVFASF IRKAADVHEV 250
    REVLGEKGKN IKIISKIENH EGVRRFDEIL EASDGIMVAR GDLGIEIPAE 300
    KVFLAQKMMI GRCNRAGKPV ICATQMLESM IKKPRPTRAE GSDVANAVLD 350
    GADCIMLSGE TAKGDYPLEA VRMQHAIARE AEAAIFHRQL FEELRRVSPL 400
    TRDPTEATAV GAVEASFKCS SGAIIVLTKS GRSAHLLSRY RPRAPIISVT 450
    RNGQTARQAH LYRGIFPVLY REAVHEAWAE DVDSRVNFAM DIGKARGFFK 500
    SGDVVIVLTG WRPGSGFTNT MRVVPVP 527
    Length:527
    Mass (Da):57,525
    Last modified:November 1, 1996 - v1
    Checksum:iA8379F0E65325A66
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03878 mRNA. Translation: AAA63581.1.
    BC079921 mRNA. Translation: AAH79921.1.
    RefSeqiNP_001084341.1. NM_001090872.1.
    UniGeneiXl.21936.

    Genome annotation databases

    GeneIDi399448.
    KEGGixla:399448.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03878 mRNA. Translation: AAA63581.1 .
    BC079921 mRNA. Translation: AAH79921.1 .
    RefSeqi NP_001084341.1. NM_001090872.1.
    UniGenei Xl.21936.

    3D structure databases

    ProteinModelPortali Q92122.
    SMRi Q92122. Positions 9-527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 100773. 1 interaction.

    Proteomic databases

    PRIDEi Q92122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399448.
    KEGGi xla:399448.

    Organism-specific databases

    CTDi 5315.
    Xenbasei XB-GENE-485877. pkm.

    Phylogenomic databases

    HOVERGENi HBG000941.
    KOi K00873.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tissue-dependent developmental expression of a cytosolic thyroid hormone protein gene in Xenopus: its role in the regulation of amphibian metamorphosis."
      Shi Y.-B., Liang V.C.-T., Parkison C., Cheng S.-Y.
      FEBS Lett. 355:61-64(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Intestine.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiKPYM_XENLA
    AccessioniPrimary (citable) accession number: Q92122
    Secondary accession number(s): Q68FC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3