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Protein

Cytochrome P450 1A3

Gene

cyp1a3

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei229 – 2291SubstrateBy similarity
Metal bindingi463 – 4631Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A3 (EC:1.14.14.1)
Short name:
CYP1A3
Alternative name(s):
CYP1A1
Gene namesi
Name:cyp1a3
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Cytochrome P450 1A3PRO_0000051642Add
BLAST

Expressioni

Tissue specificityi

Liver.

Inductioni

By 3-methylcholanthrene (3MC).

Structurei

3D structure databases

ProteinModelPortaliQ92109.
SMRiQ92109. Positions 38-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92109-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLMILPIIG SVSASEGLVA MVTLCLVYMI MKYMHTEIPE GLKRLPGPKP
60 70 80 90 100
LPIIGNMLEV HNNPHLSLTA MSERYGSVFQ IQIGMRPVVV LSGNETVRQA
110 120 130 140 150
LIKQGEDFAG RSDLYSFKFI NDGKSLAFST DKAGVWRARR KLAMSALRSF
160 170 180 190 200
ATLEGTTPEY SCALEEHVLK EGEYLVKQLT SVMDVSGSFD PFRHIVVSVA
210 220 230 240 250
NVICGMCFGR RYSHDDQELL GLVNMSDEFG QVVGSGNPAD FIPILRYLPN
260 270 280 290 300
RTMKRFMDIN DRFNNFVQKI VSEHYESYDK DNIRDITDSL IDHCEDRKLD
310 320 330 340 350
ENANIQVSDE KIVGIVNDLF GAGFDTISTA LSWAVVYLVA YPETQERLHQ
360 370 380 390 400
ELKEKVGMIR TPRLSDKINL PLLEAFILEI FRHSSFLPFT IPHCTIKDTS
410 420 430 440 450
LNGYFIPKDT CVFINQWQVN HDPELWKEPS SFNPDRFLSA DGTELNKLEG
460 470 480 490 500
EKVLVFGMGK RRCIGEAIGR NEVYLFLAIL LQRLRFQEKP GHPLDMTPEY
510 520
GLTMKHKRCQ LKASMRPWGQ EE
Length:522
Mass (Da):59,273
Last modified:May 30, 2000 - v2
Checksum:i0E83F2A5EEFE3202
GO

Sequence cautioni

The sequence AAA49550.1 differs from that shown.Chimera cDNA. Its C-terminal part has been shown to be derived from what is now known as the CYP1A1. CYP1A3 has also been called CYP1A1.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571M → V in AAD14035. (PubMed:8161204)Curated
Sequence conflicti57 – 571M → V in AAA49550. (PubMed:8161204)Curated
Sequence conflicti94 – 941N → S in AAD14035. (PubMed:8161204)Curated
Sequence conflicti94 – 941N → S in AAA49550. (PubMed:8161204)Curated
Sequence conflicti112 – 1121S → P in AAD14035. (PubMed:8161204)Curated
Sequence conflicti112 – 1121S → P in AAA49550. (PubMed:8161204)Curated
Sequence conflicti156 – 1561T → S in AAD14035. (PubMed:8161204)Curated
Sequence conflicti169 – 1691L → C in AAD14035. (PubMed:8161204)Curated
Sequence conflicti169 – 1691L → C in AAA49550. (PubMed:8161204)Curated
Sequence conflicti265 – 2651N → T in AAD14035. (PubMed:8161204)Curated
Sequence conflicti265 – 2651N → T in AAA49550. (PubMed:8161204)Curated
Sequence conflicti276 – 2761E → D in AAD14035. (PubMed:8161204)Curated
Sequence conflicti305 – 3051I → V in AAD14035. (PubMed:8161204)Curated
Sequence conflicti344 – 3441T → I in AAD14035. (PubMed:8161204)Curated
Sequence conflicti344 – 3441T → I in AAA49550. (PubMed:8161204)Curated
Sequence conflicti396 – 3961I → V in AAD14035. (PubMed:8161204)Curated
Sequence conflicti485 – 4851R → C in AAD14035. (PubMed:8161204)Curated
Sequence conflicti515 – 5228MRPWGQEE → LRHGGRKSEGHGHIYDSQHH YN in AAD14035. (PubMed:8161204)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69277 Genomic DNA. Translation: AAD14035.1.
U62796 mRNA. Translation: AAB40626.1.
M21310 mRNA. Translation: AAA49550.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S69277 Genomic DNA. Translation: AAD14035.1.
U62796 mRNA. Translation: AAB40626.1.
M21310 mRNA. Translation: AAA49550.1. Sequence problems.

3D structure databases

ProteinModelPortaliQ92109.
SMRiQ92109. Positions 38-514.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two unique CYP1 genes are expressed in response to 3-methylcholanthrene treatment in rainbow trout."
    Berndtson A.K., Chen T.T.
    Arch. Biochem. Biophys. 310:187-195(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "Cloning, sequencing and functional expression of two trout CYP1A cDNAs in yeast."
    Bailey G., You L., Harttig U.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Trout P450IA1: cDNA and deduced protein sequence, expression in liver, and evolutionary significance."
    Heilmann L.J., Sheen Y.-Y., Bigelow S.W., Nebert D.W.
    DNA 7:379-387(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiCP1A3_ONCMY
AccessioniPrimary (citable) accession number: Q92109
Secondary accession number(s): P10609, P79829
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: January 7, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.