Reviewed, UniProtKB/Swiss-Prot Q92104 (CP11B_RANCA)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 11B, mitochondrial
    EC=1.14.15.4
Alternative name(s):
    CYPXIB
    P450C11
    Steroid 11-beta-hydroxylase
    P-450(11 beta,aldo)

Gene names

Name:

CYP11B

Organism

Rana catesbeiana (Bull frog)

Taxonomic identifier

8400 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Rana › Aquarana

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has 11 beta-hydroxylation, 18-hydroxylation activities and aldosterone synthetic activity. Catalyzes the final steps of glucocorticoid and mineralocorticoid biosynthesis.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O(2) = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H(2)O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Mitochondrion Potential
	Chain	46 – 517	472	Cytochrome P450 11B, mitochondrial		PRO_0000003607
Sites
	Metal binding	465	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92104-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8F94DCD66BA6370B	FASTA	517	59,539
	10 20 30 40 50 60 MLEKTAARQI GSCLMRCRTL DTTSPLWTGF SRLSTAPLIH EAREDGSLAS QTLPYEAIPT 70 80 90 100 110 120 TGRSAWFNLF QFWRKNSFQH MHLAMEENFQ NLGPIYREKL GTHNSVNIML PQDVARLFQS 130 140 150 160 170 180 EGIFPRRMTM EAWSKHRELR NHKQGVFLLN GEAWRSDRII LNKEVLSLAG VKKFLPFLDE 190 200 210 220 230 240 AAADFVTFMK KRMSKNTRGS LTVDLYADLF RFTLEASSYV LYGQRLGLLE EHPNADTLRF 250 260 270 280 290 300 ISAVETVLKT TLPLLYYPHQ ILQLFQTRLW NEHMHAWDVI FEQADRCIQN IYQEYCLGQE 310 320 330 340 350 360 RGYSGIMAEL LLQAELPLDS IKANITELMA GGVDTTAMPL LFTLFELARN PSVQRELREE 370 380 390 400 410 420 IRKAEAQNPN DLNQLLNSLP LLKGAIKETL RLYPVGITVQ RHLIKDIVLH NYHIPAGTLV 430 440 450 460 470 480 QVGLYPMGRS PLLFQDALRY DPARWLKRED TNFKALAFGF GSRQCIGRRI AETEITLFLM 490 500 510 HMLKNFQIDT VSKDDIKTVF GFILMPEKPP LLTFRPI

References

[1]

"Frog cytochrome P-450 (11 beta,aldo), a single enzyme involved in the final steps of glucocorticoid and mineralocorticoid biosynthesis." Nonaka Y., Takemori H., Halder S.K., Sun T., Ohta M., Hatano O., Takakusu A., Okamoto M. Eur. J. Biochem. 229:249-256(1995) [PubMed: 7744036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10984 mRNA. Translation: BAA01756.1.
PIR	S69347.
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q92104.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP11B_RANCA

Accession

Primary (citable) accession number: Q92104

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)