Q920V1 (B3GL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
Short name=Beta-1,3-GalNAc-T1
EC=2.4.1.79

Alternative name(s):
Beta-1,3-galactosyltransferase 3
Short name=Beta-1,3-GalTase 3
Short name=Beta3Gal-T3
Short name=Beta3GalT3
Short name=b3Gal-T3
Beta-3-Gx-T3
Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase
Globoside synthase
UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase

Gene names

Name:	B3galnt1
Synonyms:	B3galt3, B3gt3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfers N-acetylgalactosamine onto globotriaosylceramide. Ref.2
Catalytic activity	UDP-N-acetyl-alpha-D-galactosamine + alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Cofactor	Magnesium.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Detected in brain, ovary, uterus and stomach. Ref.2
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	oligosaccharide biosynthetic process Inferred from direct assay Ref.2. Source: MGI protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Inferred from direct assay Ref.2. Source: MGI galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1		PRO_0000219154
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 43	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	44 – 331	288	Lumenal Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	326	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	4	1	A → T in strain: NJL/Msf, BLG2/Msf, MSM/Msf and SWN/Msf. Ref.1
	Natural variant	87	1	R → H in strain: SWN/Msf. Ref.1
	Natural variant	128	1	T → M in strain: CAST/Ei. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q920V1 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 387E8B0C75FC282D	FASTA	331	39,371
	10 20 30 40 50 60 MAPAVLTALP NRMSLRSLKW SLLLLSLLSF LVIWYLSLPH YNVIERVNWM YFYEYEPIYR 70 80 90 100 110 120 QDFRFTLREH SNCSHQNPFL VILVTSRPSD VKARQAIRVT WGEKKSWWGY EVLTFFLLGQ 130 140 150 160 170 180 QAEREDKTLA LSLEDEHVLY GDIIRQDFLD TYNNLTLKTI MAFRWVMEFC PNAKYIMKTD 190 200 210 220 230 240 TDVFINTGNL VKYLLNLNHS EKFFTGYPLI DNYSYRGFFH KNHISYQEYP FKVFPPYCSG 250 260 270 280 290 300 LGYIMSGDLV PRVYEMMSHV KPIKFEDVYV GICLNLLKVD IHIPEDTNLF FLYRIHLDVC 310 320 330 QLRRVIAAHG FSSKEIITFW QVMLRNTTCH Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conspicuous differences among gene genealogies of 21 nuclear genes of five Mus musculus subspecies." Liu Y., Kitano T., Koide T., Shiroishi T., Moriwaki K., Saitou N. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-4; HIS-87 AND MET-128. Strain: BFM/2Msf, BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf, Pgn2 and SWN/Msf.
[2]	"Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1,3-galactosyltransferase genes." Hennet T., Dinter A., Kuhnert P., Mattu T.S., Rudd P.M., Berger E.G. J. Biol. Chem. 273:58-65(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY. Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Embryo and Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b3GalT3

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB039154 Genomic DNA. Translation: BAB68678.1. AB039155 Genomic DNA. Translation: BAB68679.1. AB039156 Genomic DNA. Translation: BAB68680.1. AB039157 Genomic DNA. Translation: BAB68681.1. AB039158 Genomic DNA. Translation: BAB68682.1. AB039159 Genomic DNA. Translation: BAB68683.1. AB039160 Genomic DNA. Translation: BAB68684.1. AB039161 Genomic DNA. Translation: BAB68685.1. AB039162 Genomic DNA. Translation: BAB68686.1. AF029792 Genomic DNA. Translation: AAC53525.1. AK003837 mRNA. Translation: BAB23028.1. AK088407 mRNA. Translation: BAC40336.1. BC003835 mRNA. Translation: AAH03835.3.
IPI	IPI00119759.
RefSeq	NP_064410.1. NM_020026.3.
UniGene	Mm.153710.
3D structure databases
ProteinModelPortal	Q920V1.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
PTM databases
PhosphoSite	Q920V1.
Proteomic databases
PaxDb	Q920V1.
PRIDE	Q920V1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000061826; ENSMUSP00000058363; ENSMUSG00000043300.
GeneID	26879.
KEGG	mmu:26879.
UCSC	uc008pmj.1. mouse.
Organism-specific databases
CTD	8706.
MGI	MGI:1349405. B3galnt1.
Phylogenomic databases
eggNOG	NOG269758.
GeneTree	ENSGT00670000097716.
HOGENOM	HOG000059604.
HOVERGEN	HBG101817.
InParanoid	Q920V1.
KO	K00719.
OMA	CPNAKYI.
OrthoDB	EOG4PK28G.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	Q920V1.
Genevestigator	Q920V1.
GermOnline	ENSMUSG00000043300. Mus musculus.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. PTHR11214. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304695.
SOURCE	Search...

Entry information

Entry name

B3GL1_MOUSE

Accession

Primary (citable) accession number: Q920V1
Secondary accession number(s): O54906 , Q91V72, Q920V0, Q9CTE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2005
Last modified:	April 3, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program