Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

V-type proton ATPase 116 kDa subunit a isoform 4

Gene

Atp6v0a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-77387. Insulin receptor recycling.
R-MMU-917977. Transferrin endocytosis and recycling.
R-MMU-983712. Ion channel transport.

Protein family/group databases

TCDBi3.A.2.2.6. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase 116 kDa subunit a isoform 4
Short name:
V-ATPase 116 kDa isoform a4
Alternative name(s):
Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4
Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform
Gene namesi
Name:Atp6v0a4
Synonyms:Atp6n1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2153480. Atp6v0a4.

Subcellular locationi

  • Cell membrane; Multi-pass membrane protein

  • Note: Apical immunostaining present in male genital tissue. According to PubMed:11498539, clearly detectable not only on the apical surface of alpha-intercalated cells but also on the basolateral surface of beta cells.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 390390CytoplasmicSequence analysisAdd
BLAST
Transmembranei391 – 40919HelicalSequence analysisAdd
BLAST
Topological domaini410 – 4112VacuolarSequence analysis
Transmembranei412 – 42817HelicalSequence analysisAdd
BLAST
Topological domaini429 – 44315CytoplasmicSequence analysisAdd
BLAST
Transmembranei444 – 47330HelicalSequence analysisAdd
BLAST
Topological domaini474 – 53865VacuolarSequence analysisAdd
BLAST
Transmembranei539 – 55820HelicalSequence analysisAdd
BLAST
Topological domaini559 – 57618CytoplasmicSequence analysisAdd
BLAST
Transmembranei577 – 59721HelicalSequence analysisAdd
BLAST
Topological domaini598 – 64245VacuolarSequence analysisAdd
BLAST
Transmembranei643 – 66220HelicalSequence analysisAdd
BLAST
Topological domaini663 – 72058CytoplasmicSequence analysisAdd
BLAST
Transmembranei721 – 74525HelicalSequence analysisAdd
BLAST
Topological domaini746 – 76621VacuolarSequence analysisAdd
BLAST
Transmembranei767 – 80539HelicalSequence analysisAdd
BLAST
Topological domaini806 – 83328CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical part of cell Source: UniProtKB
  • apical plasma membrane Source: UniProtKB
  • brush border Source: MGI
  • brush border membrane Source: MGI
  • endosome Source: UniProtKB
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: MGI
  • plasma membrane Source: MGI
  • vacuolar proton-transporting V-type ATPase, V0 domain Source: InterPro
  • vacuolar proton-transporting V-type ATPase complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 833833V-type proton ATPase 116 kDa subunit a isoform 4PRO_0000119220Add
BLAST

Proteomic databases

MaxQBiQ920R6.
PaxDbiQ920R6.
PeptideAtlasiQ920R6.
PRIDEiQ920R6.

PTM databases

iPTMnetiQ920R6.
PhosphoSiteiQ920R6.

Expressioni

Tissue specificityi

Specifically expressed in kidney, but not in the heart, brain, spleen, lung, liver, muscle, or testis. Distribution within the kidney appears more widespread than that seen in man. High intensity staining at the surface of intercalated cells, with additional expression in the proximal tubule.

Gene expression databases

BgeeiENSMUSG00000038600.
CleanExiMM_ATP6V0A4.
GenevisibleiQ920R6. MM.

Interactioni

Subunit structurei

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228267. 1 interaction.
IntActiQ920R6. 2 interactions.
MINTiMINT-4140035.
STRINGi10090.ENSMUSP00000039381.

Structurei

3D structure databases

ProteinModelPortaliQ920R6.
SMRiQ920R6. Positions 10-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiQ920R6.
KOiK02154.
OMAiWTMVMNS.
OrthoDBiEOG091G01BI.
PhylomeDBiQ920R6.
TreeFamiTF300346.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q920R6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVFRSEEM CLSQVFLQVE AAYCCVAELG ELGLVQFKDL NANVNSFQRK
60 70 80 90 100
FVNEVRRCES LERILRFLED EMQNEILIQV PEKDAETPLP REMITLETTL
110 120 130 140 150
EKLEGELQEA NQSHQALKKS FLELTELKYL LKKTQDFFET ETNLGEDFFV
160 170 180 190 200
EDTSGLLELR TIPAFMTGKL GFTAGVINRE RMASFERLLW RVCRGNVYLK
210 220 230 240 250
FSEMDTLLED PVTKEEIKKN IFIIFYQGEQ LRLKIKKICD GFRATIYPCP
260 270 280 290 300
EHAAERREML TSVNVRLEDL ITVITQTESH RQRLLQEAAA NWHSWVIKVQ
310 320 330 340 350
KMKAVYHVLN MCNIDVTQQC IIAEIWFPVA DTRHIKKALE QGMELSGSSM
360 370 380 390 400
IPIMTEVETK TDPPTFNRTN KFTAGFQNIV DAYGVGSYRE INPAPYTIIT
410 420 430 440 450
FPFLFAVMFG DCGHGMVMLM AALWMVLNER HLLAQKSTNE MWNIFFNGRY
460 470 480 490 500
LILLMGIFSI YTGLIYNDCF SKSFNIFGSS WSVQPMFRNG TWNTHIVENS
510 520 530 540 550
PYLQLDPAIP GVYSGNPYPF GIDPIWNLAS NKLTFLNSYK MKMSVILGIA
560 570 580 590 600
HMIFGVILSL FNHIYFRRTL NIILQFIPEM IFMLSLFGYL VFMIIFKWCR
610 620 630 640 650
YDAHTSRKAP SILIHFIGMF LFDYDDSSNA PLYGHQQEVQ TFFVIIALVS
660 670 680 690 700
VPWMLLIKPF VLRAKHQKSQ LQSFTIHEDA VEGDHSGHSS KKTAGAHGMK
710 720 730 740 750
DGHEEEFNFG DIFVHQAIHT IEYCLGCISN TASYLRLWAL SLAHAELSEV
760 770 780 790 800
LWTMVMSIGL RLQGWAGLVG VFIIFAVFAV LTVAILLVME GLSAFLHALR
810 820 830
LHWVEFQNKF YEGAGSKFSP FSFKHVLEGT AEE
Length:833
Mass (Da):95,605
Last modified:December 1, 2001 - v1
Checksum:iBADEE53790C83B45
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41V → A in AAN45855 (Ref. 3) Curated
Sequence conflicti161 – 1611T → A in AAL30435 (PubMed:11495928).Curated
Sequence conflicti625 – 6251D → N in AAN45855 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050903 mRNA. Translation: BAB47243.1.
AF326316 mRNA. Translation: AAL30435.1.
AF435090 mRNA. Translation: AAN45855.1.
BC046979 mRNA. Translation: AAH46979.1.
CCDSiCCDS20010.1.
RefSeqiNP_536715.3. NM_080467.3.
UniGeneiMm.462308.

Genome annotation databases

EnsembliENSMUST00000040259; ENSMUSP00000039381; ENSMUSG00000038600.
ENSMUST00000114908; ENSMUSP00000110558; ENSMUSG00000038600.
GeneIDi140494.
KEGGimmu:140494.
UCSCiuc009bjo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050903 mRNA. Translation: BAB47243.1.
AF326316 mRNA. Translation: AAL30435.1.
AF435090 mRNA. Translation: AAN45855.1.
BC046979 mRNA. Translation: AAH46979.1.
CCDSiCCDS20010.1.
RefSeqiNP_536715.3. NM_080467.3.
UniGeneiMm.462308.

3D structure databases

ProteinModelPortaliQ920R6.
SMRiQ920R6. Positions 10-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228267. 1 interaction.
IntActiQ920R6. 2 interactions.
MINTiMINT-4140035.
STRINGi10090.ENSMUSP00000039381.

Protein family/group databases

TCDBi3.A.2.2.6. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiQ920R6.
PhosphoSiteiQ920R6.

Proteomic databases

MaxQBiQ920R6.
PaxDbiQ920R6.
PeptideAtlasiQ920R6.
PRIDEiQ920R6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040259; ENSMUSP00000039381; ENSMUSG00000038600.
ENSMUST00000114908; ENSMUSP00000110558; ENSMUSG00000038600.
GeneIDi140494.
KEGGimmu:140494.
UCSCiuc009bjo.2. mouse.

Organism-specific databases

CTDi50617.
MGIiMGI:2153480. Atp6v0a4.

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiQ920R6.
KOiK02154.
OMAiWTMVMNS.
OrthoDBiEOG091G01BI.
PhylomeDBiQ920R6.
TreeFamiTF300346.

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-77387. Insulin receptor recycling.
R-MMU-917977. Transferrin endocytosis and recycling.
R-MMU-983712. Ion channel transport.

Miscellaneous databases

PROiQ920R6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038600.
CleanExiMM_ATP6V0A4.
GenevisibleiQ920R6. MM.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVPP4_MOUSE
AccessioniPrimary (citable) accession number: Q920R6
Secondary accession number(s): Q8CJ79, Q920B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.