Q920R3 (FADS1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fatty acid desaturase 1
EC=1.14.19.-

Alternative name(s):
Delta(5) fatty acid desaturase
Short name=D5D
Short name=Delta(5) desaturase
Short name=Delta-5 desaturase

Gene names

Name:

Fads1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively By similarity.
Pathway	Lipid metabolism; polyunsaturated fatty acid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Tissue specificity	Highly abundant in adrenal gland and mammary tissue and moderately in liver, kidney, lung, spleen, thymus, brain, and eye. Ref.1
Induction	Regulated by dietary vitamin A and exogenous retinoic acid in liver. Ref.1
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the fatty acid desaturase family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism Transport
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 8487621. Source: RGD arachidonic acid metabolic process Inferred from mutant phenotype Ref.1. Source: RGD cellular response to starvation Inferred from electronic annotation. Source: Compara electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW response to insulin stimulus Inferred from expression pattern PubMed 16099631. Source: RGD response to organic cyclic compound Inferred from expression pattern PubMed 16099631. Source: RGD response to stress Inferred from expression pattern PubMed 8115350. Source: RGD response to sucrose stimulus Inferred from expression pattern PubMed 14506836. Source: RGD response to vitamin A Inferred from expression pattern Ref.1. Source: RGD unsaturated fatty acid biosynthetic process Inferred from direct assay Ref.1. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay PubMed 7770068. Source: RGD
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Fatty acid desaturase 1		PRO_0000307099
Regions
	Topological domain	1 – 132	132	Cytoplasmic Potential
	Transmembrane	133 – 153	21	Helical; Potential
	Topological domain	154 – 161	8	Lumenal Potential
	Transmembrane	162 – 180	19	Helical; Potential
	Topological domain	181 – 268	88	Cytoplasmic Potential
	Transmembrane	269 – 289	21	Helical; Potential
	Topological domain	290 – 308	19	Lumenal Potential
	Transmembrane	309 – 329	21	Helical; Potential
	Topological domain	330 – 447	118	Cytoplasmic Potential
	Domain	19 – 97	79	Cytochrome b5 heme-binding
	Motif	182 – 186	5	Histidine box-1
	Motif	219 – 223	5	Histidine box-2
	Motif	385 – 389	5	Histidine box-3
Experimental info
	Sequence conflict	73	1	P → R in AAG35068. Ref.1
	Sequence conflict	85	1	R → E in AAG35068. Ref.1
	Sequence conflict	101	1	P → S in AAG35068. Ref.1
	Sequence conflict	391	1	F → L in AAG35068. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q920R3 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 764D7D7C9AA3F7BE	FASTA	447	52,482
	10 20 30 40 50 60 MAPDPVQTPD PASAQLRQMR YFTWEEVAQR SGREKERWLV IDRKVYNISD FSRRHPGGSR 70 80 90 100 110 120 VISHYAGQDA TDPFVAFHIN KGLVRKYMNS LLIGELAPEQ PSFEPTKNKA LTDEFRELRA 130 140 150 160 170 180 TVERMGLMKA NHLFFLFYLL HILLLDVAAW LTLWIFGTSL VPFTLCAVLL STVQAQAGWL 190 200 210 220 230 240 QHDFGHLSVF STSTWNHLVH HFVIGHLKGA PASWWNHMHF QHHAKPNCFR KDPDINMHPL 250 260 270 280 290 300 FFALGKVLSV ELGKEKKKHM PYNHQHKYFF LIGPPALLPL YFQWYIFYFV VQRKKWVDLA 310 320 330 340 350 360 WMLSFYVRVF FTYMPLLGLK GLLCLFFIVR FLESNWFVWV TQMNHIPMHI DHDRNVDWVS 370 380 390 400 410 420 TQLQATCNVH QSAFNNWFSG HLNFQIEHHL FPTMPRHNYH KVAPLVQSLC AKYGIKYESK 430 440 PLLTAFADIV YSLKESGQLW LDAYLHQ

References

[1]	"Fatty acid Delta(5)-desaturase mRNA is regulated by dietary vitamin A and exogenous retinoic acid in liver of adult rats." Zolfaghari R., Cifelli C.J., Banta M.D., Ross A.C. Arch. Biochem. Biophys. 391:8-15(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: Lewis. Tissue: Liver.
[2]	"Cloning and expression of rat liver delta-5 fatty acid desaturase." Inagaki K., Aki T., Shimada Y., Kawamoto S., Shigeta S., Ono K., Suzuki O. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF320509 mRNA. Translation: AAG35068.1. AB052085 mRNA. Translation: BAB69054.1.
IPI	IPI00324109.
RefSeq	NP_445897.2. NM_053445.2.
UniGene	Rn.28161.
3D structure databases
HSSP	HSSP built from PDB template 1B5M based on UniProtKB P04166.
ProteinModelPortal	Q920R3.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000027834.
PTM databases
PhosphoSite	Q920R3.
Proteomic databases
PaxDb	Q920R3.
PRIDE	Q920R3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000027834; ENSRNOP00000027834; ENSRNOG00000020480.
GeneID	84575.
KEGG	rno:84575.
UCSC	RGD:621678. rat.
Organism-specific databases
CTD	3992.
RGD	621678. Fads1.
Phylogenomic databases
eggNOG	COG5274.
GeneTree	ENSGT00510000046574.
HOGENOM	HOG000012997.
HOVERGEN	HBG002839.
InParanoid	Q920R3.
KO	K10224.
OMA	RYFTWEE.
OrthoDB	EOG4G7BZ9.
Enzyme and pathway databases
UniPathway	UPA00658.
Gene expression databases
Genevestigator	Q920R3.
Family and domain databases
Gene3D	3.10.120.10. 1 hit.
InterPro	IPR001199. Cyt_B5-like_heme/steroid-bd. IPR012171. Fatty_acid/sphinglp_desaturase. IPR005804. Fatty_acid_desaturase-1. [Graphical view]
Pfam	PF00173. Cyt-b5. 1 hit. PF00487. FA_desaturase. 1 hit. [Graphical view]
PIRSF	PIRSF015921. FA_sphinglp_des. 1 hit.
PRINTS	PR00363. CYTOCHROMEB5.
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. False negative. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q920R3.
ChEMBL	CHEMBL5727.
NextBio	617125.

Entry information

Entry name

FADS1_RAT

Accession

Primary (citable) accession number: Q920R3
Secondary accession number(s): Q9EPV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program