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Protein

Influenza virus NS1A-binding protein homolog

Gene

Ivns1abp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cell division and in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats. Protects cells from cell death induced by actin destabilization; Protects neurons from dendritic spines and actin filaments damage induced by the actin-destabilizing cytochalasin B when overexpressed. Plays a role in protecting the heart from doxorubicin-induced cardiomyopathy. Activates Erk signaling pathway when overexpressed in cultured cell lines.5 Publications

GO - Biological processi

  • negative regulation of intrinsic apoptotic signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Influenza virus NS1A-binding protein homolog
Short name:
NS1-BP
Short name:
NS1-binding protein homolog
Alternative name(s):
Kelch family protein Nd1-L
ND1-L2
Nd1-S
Gene namesi
Name:Ivns1abp
Synonyms:Kiaa0850, Nd1, Nd1L, Nd1S, Ns1, Ns1bp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2152389. Ivns1abp.

Subcellular locationi

Isoform 1 :

GO - Cellular componenti

  • actin cytoskeleton Source: InterPro
  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop normally with no gross abnormalities. However, they display marked sensitivity to doxorubicin cardiotoxicity with increased number of cardiomyocytes apoptosis. Analysis of hearts from knockout mice reveal vacuolization and edema of cardiomaycytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002850781 – 642Influenza virus NS1A-binding protein homologAdd BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei246PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1
Modified residuei322PhosphoserineCombined sources1
Modified residuei336PhosphoserineBy similarity1
Modified residuei338PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ920Q8.
PeptideAtlasiQ920Q8.
PRIDEiQ920Q8.

PTM databases

iPTMnetiQ920Q8.
PhosphoSitePlusiQ920Q8.

Expressioni

Tissue specificityi

Ubiquitous expression. In the heart, the highest expression is detected in the ventricles and the lowest in the atria. Expressed in dendrites and spines in neurons.3 Publications

Developmental stagei

Barely detected in the heart at E15.5, but clearly expressed in newborn heart with increased amount up to 8 weeks of age.1 Publication

Inductioni

Decreased expression in various organs and cultured cell lines by doxorubicin treatment which may reduce mRNA stability.2 Publications

Gene expression databases

BgeeiENSMUSG00000023150.
CleanExiMM_IVNS1ABP.
ExpressionAtlasiQ920Q8. baseline and differential.
GenevisibleiQ920Q8. MM.

Interactioni

Subunit structurei

Homodimer; through the BTB domain. Interacts with AHR/Aryl hydrocarbon receptor (By similarity).By similarity

Protein-protein interaction databases

BioGridi228184. 5 interactors.
IntActiQ920Q8. 2 interactors.
MINTiMINT-1634681.
STRINGi10090.ENSMUSP00000023918.

Structurei

3D structure databases

ProteinModelPortaliQ920Q8.
SMRiQ920Q8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 99BTBPROSITE-ProRule annotationAdd BLAST68
Domaini134 – 233BACKAdd BLAST100
Repeati369 – 415Kelch 1Add BLAST47
Repeati416 – 463Kelch 2Add BLAST48
Repeati465 – 512Kelch 3Add BLAST48
Repeati513 – 559Kelch 4Add BLAST47
Repeati561 – 606Kelch 5Add BLAST46
Repeati608 – 642Kelch 6Add BLAST35

Domaini

When the BTB domain is lacking, AHR signaling induction promoted by IVNS1ABP is massively increased; Thus, the BTB domain inhibits AHR signaling induced by IVNS1ABP.By similarity

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000119153.
HOVERGENiHBG082407.
InParanoidiQ920Q8.
KOiK15046.
OMAiPNIDDWI.
OrthoDBiEOG091G024F.
PhylomeDBiQ920Q8.
TreeFamiTF329218.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015916. Gal_Oxidase_b-propeller.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR029849. NS1BP.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24411:SF7. PTHR24411:SF7. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q920Q8-1) [UniParc]FASTAAdd to basket
Also known as: Nd1-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIPNGYLMFE DENFIESSVA KLNALRKSGQ FCDVRLQVCG HEMLAHRAVL
60 70 80 90 100
ACCSPYLFEI FNSDSDPHGV SHVKLDDLNP EAVEVLLNYA YTAQLKADKE
110 120 130 140 150
LVKDVYSAAK KLKMDRVKQV CGDYLLSRMD VTSCISYRNF ASCMGDSRLL
160 170 180 190 200
NKVDAYIQEH LLQISEEEEF LKLPRLKLEV MLEDNVCLPS NGKLYTKVIN
210 220 230 240 250
WVQRSIWENG DSLEELMEEV QTLYYSADHK LLDGNPLDGQ AEVFGSDDDH
260 270 280 290 300
IQFVQKKPPR ENGHKQISGS STGCLSSPNA SMQSPKHEWK IVASEKTSNN
310 320 330 340 350
TYLCLAVLDS TFCVIFLHGR NSPQSSPTST PKLSKSLSFE MQPDELLEKP
360 370 380 390 400
MSPMQYARSG LGTAEMNGKL IAAGGYNREE CLRTVECYDP HTDHWSFLAP
410 420 430 440 450
MRTPRARFQM AVLMGQLYVV GGSNGHSDDL SCGEMYDPNI DDWTPVPELR
460 470 480 490 500
TNRCNAGVCA LNGKLYIVGG SDPYGQKGLK NCDVFDPVTK SWTSCAPLNI
510 520 530 540 550
RRHQSAVCEL GGYLYIIGGA ESWNCLNTVE RYNPENNTWT LIAPMNVARR
560 570 580 590 600
GAGVAVLDGK LFVGGGFDGS HAISCVEMYD PTRNEWKMMG NMTSPRSNAG
610 620 630 640
ITTVGNTIYA VGGFDGNEFL NTVEVYNPQS NEWSPYTKIF QF
Length:642
Mass (Da):71,581
Last modified:May 1, 2007 - v2
Checksum:i7240360079C16B50
GO
Isoform 2 (identifier: Q920Q8-2) [UniParc]FASTAAdd to basket
Also known as: Nd1-S

The sequence of this isoform differs from the canonical sequence as follows:
     221-221: Q → Y
     222-642: Missing.

Show »
Length:221
Mass (Da):25,333
Checksum:i9A8EC762FEB9A7E5
GO
Isoform 3 (identifier: Q920Q8-3) [UniParc]FASTAAdd to basket
Also known as: Nd1-L2

The sequence of this isoform differs from the canonical sequence as follows:
     179-218: Missing.

Show »
Length:602
Mass (Da):66,916
Checksum:i1E9680A37A479696
GO
Isoform 4 (identifier: Q920Q8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-219: Missing.

Note: No experimental confirmation available.
Show »
Length:600
Mass (Da):66,673
Checksum:iAFE7313C1D09DE2D
GO

Sequence cautioni

The sequence BAC98039 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106Y → H in BAE26822 (PubMed:16141072).Curated1
Sequence conflicti201W → R in BAE34935 (PubMed:16141072).Curated1
Sequence conflicti285P → T in BAE27084 (PubMed:16141072).Curated1
Sequence conflicti295E → K in BAE27084 (PubMed:16141072).Curated1
Sequence conflicti544P → S in AAH04092 (PubMed:15489334).Curated1
Sequence conflicti548A → S in BAE27084 (PubMed:16141072).Curated1
Sequence conflicti549R → K in BAB69058 (PubMed:12213805).Curated1
Sequence conflicti589M → I in BAE27084 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024810178 – 219Missing in isoform 4. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_024811179 – 218Missing in isoform 3. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_024812221Q → Y in isoform 2. 3 Publications1
Alternative sequenceiVSP_024813222 – 642Missing in isoform 2. 3 PublicationsAdd BLAST421

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055737 mRNA. Translation: BAB69058.1.
AB055738 mRNA. Translation: BAB69059.1.
DQ914522 mRNA. Translation: ABI84241.1.
AK129229 mRNA. Translation: BAC98039.1. Different initiation.
AK007291 mRNA. Translation: BAB24936.1.
AK144835 mRNA. Translation: BAE26089.1.
AK145071 mRNA. Translation: BAE26221.1.
AK146001 mRNA. Translation: BAE26822.1.
AK146329 mRNA. Translation: BAE27084.1.
AK146494 mRNA. Translation: BAE27212.1.
AK146645 mRNA. Translation: BAE27326.1.
AK159254 mRNA. Translation: BAE34935.1.
BC004092 mRNA. Translation: AAH04092.1.
BC040250 mRNA. Translation: AAH40250.1.
CCDSiCCDS15358.1. [Q920Q8-4]
CCDS15359.1. [Q920Q8-1]
CCDS35735.1. [Q920Q8-2]
RefSeqiNP_001034600.1. NM_001039511.1. [Q920Q8-2]
NP_001034601.1. NM_001039512.1. [Q920Q8-4]
NP_473443.2. NM_054102.2. [Q920Q8-1]
UniGeneiMm.33764.

Genome annotation databases

EnsembliENSMUST00000023918; ENSMUSP00000023918; ENSMUSG00000023150. [Q920Q8-1]
ENSMUST00000097543; ENSMUSP00000095150; ENSMUSG00000023150. [Q920Q8-4]
ENSMUST00000111887; ENSMUSP00000107518; ENSMUSG00000023150. [Q920Q8-2]
ENSMUST00000186745; ENSMUSP00000140708; ENSMUSG00000023150. [Q920Q8-2]
GeneIDi117198.
KEGGimmu:117198.
UCSCiuc007cym.1. mouse. [Q920Q8-2]
uc007cyn.1. mouse. [Q920Q8-1]
uc007cyo.1. mouse. [Q920Q8-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055737 mRNA. Translation: BAB69058.1.
AB055738 mRNA. Translation: BAB69059.1.
DQ914522 mRNA. Translation: ABI84241.1.
AK129229 mRNA. Translation: BAC98039.1. Different initiation.
AK007291 mRNA. Translation: BAB24936.1.
AK144835 mRNA. Translation: BAE26089.1.
AK145071 mRNA. Translation: BAE26221.1.
AK146001 mRNA. Translation: BAE26822.1.
AK146329 mRNA. Translation: BAE27084.1.
AK146494 mRNA. Translation: BAE27212.1.
AK146645 mRNA. Translation: BAE27326.1.
AK159254 mRNA. Translation: BAE34935.1.
BC004092 mRNA. Translation: AAH04092.1.
BC040250 mRNA. Translation: AAH40250.1.
CCDSiCCDS15358.1. [Q920Q8-4]
CCDS15359.1. [Q920Q8-1]
CCDS35735.1. [Q920Q8-2]
RefSeqiNP_001034600.1. NM_001039511.1. [Q920Q8-2]
NP_001034601.1. NM_001039512.1. [Q920Q8-4]
NP_473443.2. NM_054102.2. [Q920Q8-1]
UniGeneiMm.33764.

3D structure databases

ProteinModelPortaliQ920Q8.
SMRiQ920Q8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228184. 5 interactors.
IntActiQ920Q8. 2 interactors.
MINTiMINT-1634681.
STRINGi10090.ENSMUSP00000023918.

PTM databases

iPTMnetiQ920Q8.
PhosphoSitePlusiQ920Q8.

Proteomic databases

PaxDbiQ920Q8.
PeptideAtlasiQ920Q8.
PRIDEiQ920Q8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023918; ENSMUSP00000023918; ENSMUSG00000023150. [Q920Q8-1]
ENSMUST00000097543; ENSMUSP00000095150; ENSMUSG00000023150. [Q920Q8-4]
ENSMUST00000111887; ENSMUSP00000107518; ENSMUSG00000023150. [Q920Q8-2]
ENSMUST00000186745; ENSMUSP00000140708; ENSMUSG00000023150. [Q920Q8-2]
GeneIDi117198.
KEGGimmu:117198.
UCSCiuc007cym.1. mouse. [Q920Q8-2]
uc007cyn.1. mouse. [Q920Q8-1]
uc007cyo.1. mouse. [Q920Q8-4]

Organism-specific databases

CTDi10625.
MGIiMGI:2152389. Ivns1abp.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000119153.
HOVERGENiHBG082407.
InParanoidiQ920Q8.
KOiK15046.
OMAiPNIDDWI.
OrthoDBiEOG091G024F.
PhylomeDBiQ920Q8.
TreeFamiTF329218.

Miscellaneous databases

ChiTaRSiIvns1abp. mouse.
PROiQ920Q8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023150.
CleanExiMM_IVNS1ABP.
ExpressionAtlasiQ920Q8. baseline and differential.
GenevisibleiQ920Q8. MM.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015916. Gal_Oxidase_b-propeller.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR029849. NS1BP.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24411:SF7. PTHR24411:SF7. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNS1BP_MOUSE
AccessioniPrimary (citable) accession number: Q920Q8
Secondary accession number(s): Q06BK6
, Q3TXI1, Q3UJE3, Q3UJS1, Q3UKH9, Q3UMK9, Q6ZQ34, Q99KN0, Q9D978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Transgenic mice overexpressing Ivns1abp develop normally with no gross abnormalities up to 7-month old. However, they display a marked resistance to the cardiotoxic effect of doxorubicin which is an anti-neoplastic agent known to affect actin skeleton and an effective drug for cancer therapy with cardiotoxicity as side effect. Overexpression of Ivns1abp in the heart protect cardiomyocytes from apoptosis and improved survival rate after doxorubicin injection.
Disorganized actin skeleton is observed in cells transfected with isoform 2 (Nd1-S), which lacks the six kelch repeats.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.