Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA-binding protein Musashi homolog 2

Gene

Msi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.2 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI
  • poly(U) RNA binding Source: MGI
  • single-stranded RNA binding Source: MGI

GO - Biological processi

  • stem cell development Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein Musashi homolog 2
Short name:
Musashi-2
Gene namesi
Name:Msi2
Synonyms:Msi2h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1923876. Msi2.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Associated with polysomes.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • polysome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346RNA-binding protein Musashi homolog 2PRO_0000081653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei6 – 61PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ920Q6.
MaxQBiQ920Q6.
PaxDbiQ920Q6.
PRIDEiQ920Q6.

PTM databases

iPTMnetiQ920Q6.
PhosphoSiteiQ920Q6.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in proliferating neural precursor cells.2 Publications

Inductioni

Up-regulated in astrocytes after brain injury.1 Publication

Gene expression databases

BgeeiQ920Q6.
CleanExiMM_MSI2.
ExpressionAtlasiQ920Q6. baseline and differential.
GenevisibleiQ920Q6. MM.

Interactioni

Protein-protein interaction databases

IntActiQ920Q6. 4 interactions.
MINTiMINT-4102465.
STRINGi10090.ENSMUSP00000090470.

Structurei

3D structure databases

ProteinModelPortaliQ920Q6.
SMRiQ920Q6. Positions 22-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 11191RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini110 – 18778RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi253 – 2608Poly-Ala

Sequence similaritiesi

Belongs to the Musashi family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4205. Eukaryota.
ENOG410YA8Z. LUCA.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ920Q6.
KOiK14411.
OMAiIDMYSSG.
OrthoDBiEOG715Q6V.
PhylomeDBiQ920Q6.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q920Q6-1) [UniParc]FASTAAdd to basket

Also known as: Msi2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEANGSPGTS GSANDSQHDP GKMFIGGLSW QTSPDSLRDY FSKFGEIREC
60 70 80 90 100
MVMRDPTTKR SRGFGFVTFA DPASVDKVLG QPHHELDSKT IDPKVAFPRR
110 120 130 140 150
AQPKMVTRTK KIFVGGLSAN TVVEDVKQYF EQFGKVEDAM LMFDKTTNRH
160 170 180 190 200
RGFGFVTFEN EDVVEKVCEI HFHEINNKMV ECKKAQPKEV MFPPGTRGRA
210 220 230 240 250
RGLPYTMDAF MLGMGMLGYP NFVATYGRGY PGFAPSYGYQ FPGFPAAAYG
260 270 280 290 300
PVAAAAVAAA RGSVLNSYSA QPNFGAPASP AGSNPARPGG FPGANSPGPV
310 320 330 340
ADLYGPASQD SGVGNYISAA SPQPGSGFGH GIAGPLIATA FTNGYH
Length:346
Mass (Da):36,939
Last modified:December 1, 2001 - v1
Checksum:iC2C75F2D7077AF61
GO
Isoform 2 (identifier: Q920Q6-2) [UniParc]FASTAAdd to basket

Also known as: Msi2S

The sequence of this isoform differs from the canonical sequence as follows:
     264-281: Missing.

Show »
Length:328
Mass (Da):35,166
Checksum:i6EB14C42397CA627
GO
Isoform 3 (identifier: Q920Q6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     264-282: VLNSYSAQPNFGAPASPAG → GRKVYGAGGSQACLVCRGR
     283-346: Missing.

Note: No experimental confirmation available.
Show »
Length:282
Mass (Da):30,893
Checksum:i156B6685DC0EE4B8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei264 – 28219VLNSY…ASPAG → GRKVYGAGGSQACLVCRGR in isoform 3. 1 PublicationVSP_011172Add
BLAST
Alternative sequencei264 – 28118Missing in isoform 2. 1 PublicationVSP_011173Add
BLAST
Alternative sequencei283 – 34664Missing in isoform 3. 1 PublicationVSP_011174Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056102 mRNA. Translation: BAB69484.1.
AB056103 mRNA. Translation: BAB69485.1.
BK001483 mRNA. Translation: DAA01567.1.
AK049637 mRNA. Translation: BAC33851.1.
AK049688 mRNA. Translation: BAC33873.1.
AK051269 mRNA. Translation: BAC34584.1.
CCDSiCCDS56796.1. [Q920Q6-1]
RefSeqiNP_473384.1. NM_054043.3. [Q920Q6-1]
XP_006534505.1. XM_006534442.2. [Q920Q6-2]
XP_006536532.1. XM_006536469.1. [Q920Q6-2]
UniGeneiMm.400451.
Mm.428433.
Mm.474737.

Genome annotation databases

EnsembliENSMUST00000092794; ENSMUSP00000090470; ENSMUSG00000069769. [Q920Q6-1]
ENSMUST00000107909; ENSMUSP00000103542; ENSMUSG00000069769. [Q920Q6-2]
GeneIDi76626.
KEGGimmu:76626.
UCSCiuc007kvl.2. mouse. [Q920Q6-1]
uc007kvn.2. mouse. [Q920Q6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056102 mRNA. Translation: BAB69484.1.
AB056103 mRNA. Translation: BAB69485.1.
BK001483 mRNA. Translation: DAA01567.1.
AK049637 mRNA. Translation: BAC33851.1.
AK049688 mRNA. Translation: BAC33873.1.
AK051269 mRNA. Translation: BAC34584.1.
CCDSiCCDS56796.1. [Q920Q6-1]
RefSeqiNP_473384.1. NM_054043.3. [Q920Q6-1]
XP_006534505.1. XM_006534442.2. [Q920Q6-2]
XP_006536532.1. XM_006536469.1. [Q920Q6-2]
UniGeneiMm.400451.
Mm.428433.
Mm.474737.

3D structure databases

ProteinModelPortaliQ920Q6.
SMRiQ920Q6. Positions 22-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ920Q6. 4 interactions.
MINTiMINT-4102465.
STRINGi10090.ENSMUSP00000090470.

PTM databases

iPTMnetiQ920Q6.
PhosphoSiteiQ920Q6.

Proteomic databases

EPDiQ920Q6.
MaxQBiQ920Q6.
PaxDbiQ920Q6.
PRIDEiQ920Q6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092794; ENSMUSP00000090470; ENSMUSG00000069769. [Q920Q6-1]
ENSMUST00000107909; ENSMUSP00000103542; ENSMUSG00000069769. [Q920Q6-2]
GeneIDi76626.
KEGGimmu:76626.
UCSCiuc007kvl.2. mouse. [Q920Q6-1]
uc007kvn.2. mouse. [Q920Q6-3]

Organism-specific databases

CTDi124540.
MGIiMGI:1923876. Msi2.

Phylogenomic databases

eggNOGiKOG4205. Eukaryota.
ENOG410YA8Z. LUCA.
GeneTreeiENSGT00730000110584.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ920Q6.
KOiK14411.
OMAiIDMYSSG.
OrthoDBiEOG715Q6V.
PhylomeDBiQ920Q6.

Miscellaneous databases

ChiTaRSiMsi2. mouse.
PROiQ920Q6.
SOURCEiSearch...

Gene expression databases

BgeeiQ920Q6.
CleanExiMM_MSI2.
ExpressionAtlasiQ920Q6. baseline and differential.
GenevisibleiQ920Q6. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS."
    Sakakibara S., Nakamura Y., Satoh H., Okano H.
    J. Neurosci. 21:8091-8107(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: C57BL/6J and ICR.
    Tissue: Brain.
  2. "Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice."
    Aubert J., Stavridis M.P., Tweedie S., O'Reilly M., Vierlinger K., Li M., Ghazal P., Pratt T., Mason J.O., Roy D., Smith A.
    Proc. Natl. Acad. Sci. U.S.A. 100:11836-11841(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: 129/Ola.
    Tissue: Embryo.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J and ICR.
    Tissue: Brain and Spinal ganglion.
  4. "RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation."
    Sakakibara S., Nakamura Y., Yoshida T., Shibata S., Koike M., Takano H., Ueda S., Uchiyama Y., Noda T., Okano H.
    Proc. Natl. Acad. Sci. U.S.A. 99:15194-15199(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiMSI2H_MOUSE
AccessioniPrimary (citable) accession number: Q920Q6
Secondary accession number(s): Q8BQ90, Q920Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.