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Protein

Vacuolar protein sorting-associated protein 16 homolog

Gene

Vps16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for recruitment of VPS33A to the HOPS complex. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS33A but not VPS33B. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.By similarity

GO - Molecular functioni

  • actin binding Source: MGI

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 16 homolog
Short name:
mVPS16
Gene namesi
Name:Vps16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2136772. Vps16.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52N → K: Down-regulation of VPS16 expression in mice; mice exhibited obvious abnormality in the behavior and significantly impaired motor function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000658891 – 839Vacuolar protein sorting-associated protein 16 homologAdd BLAST839

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4Nitrated tyrosineCombined sources1

Keywords - PTMi

Nitration

Proteomic databases

EPDiQ920Q4.
PaxDbiQ920Q4.
PeptideAtlasiQ920Q4.
PRIDEiQ920Q4.

PTM databases

iPTMnetiQ920Q4.
PhosphoSitePlusiQ920Q4.

Expressioni

Gene expression databases

CleanExiMM_VPS16.

Interactioni

Subunit structurei

Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1 (PubMed:25266290). Interacts with RAB5C (PubMed:25266290). Interacts with STX17, MON1B (By similarity). Associates with adaptor protein complex 3 (AP-3) and clathrin:AP-3 complexes (PubMed:21411634).By similarity1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VIPAS39Q9H9C14EBI-775797,EBI-749080From Homo sapiens.

GO - Molecular functioni

  • actin binding Source: MGI

Protein-protein interaction databases

IntActiQ920Q4. 4 interactors.
MINTiMINT-4140055.
STRINGi10090.ENSMUSP00000028900.

Structurei

3D structure databases

ProteinModelPortaliQ920Q4.
SMRiQ920Q4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni642 – 736Interaction with VPS33ABy similarityAdd BLAST95

Sequence similaritiesi

Belongs to the VPS16 family.Curated

Phylogenomic databases

eggNOGiKOG2280. Eukaryota.
ENOG410XQ8X. LUCA.
HOGENOMiHOG000177385.
HOVERGENiHBG056306.
InParanoidiQ920Q4.
PhylomeDBiQ920Q4.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiView protein in InterPro
IPR011042. 6-blade_b-propeller_TolB-like.
IPR016534. VPS16.
IPR006925. Vps16_C.
IPR006926. Vps16_N.
PfamiView protein in Pfam
PF04840. Vps16_C. 1 hit.
PF04841. Vps16_N. 1 hit.
PIRSFiPIRSF007949. VPS16. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q920Q4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDCYTANWNP LGDSAFYRKY ELYSMDWDLK EELKDCLVAA APYGGPIALL
60 70 80 90 100
RNCWRKEKAA SVRPVLEIYS ASGLPLASLL WKSGPVVALG WSAEEELLCV
110 120 130 140 150
QEDGAVLVYG LHGDFRRHFS MGNEVLQNRV LDARIFHTEF GSGVAILTGA
160 170 180 190 200
YRFTLSANVG DLKLRRMPEV PGLQSAPSCW TTLCHDRVPH ILLAVGPDLY
210 220 230 240 250
LLDHATCSAV TPAGLAPGVS SFLQMAVSFT YRYLALFTDT GYIWMGTASL
260 270 280 290 300
KEKLCEFNCN IRAPPKQMVW CSRPRSKERA VVVAWERRLM VVGNAPESIQ
310 320 330 340 350
FVLDEDSYLV PELDGVRIFS RSTHEFLHEV PVASEEIFKI ASMAPGALLL
360 370 380 390 400
EAQKEYEKES QKADEYLREI QELGQLIQAV QQCIEAAGHE HQPDMQKSLL
410 420 430 440 450
RAASFGKCFL DRFPPDSFVH MCQDLRVLNA IRDYHIGIPL TYTQYKQLTI
460 470 480 490 500
QVLLDRLVLR RLYPLAIQIC EYLRLPEVQG VSRILAHWAC YKARAWDMRD
510 520 530 540 550
EDVARAINQK LGDTPGVSYS DIAARAYGCG RTELAIKLLE YEPRSGEQVP
560 570 580 590 600
LLLKMKRSKL ALSKAIESGD TDLVFTVLLH LKNELNRGDF FMTLRNQPMA
610 620 630 640 650
LSLYRQFCKH QELDTLKDLY NQDDNHQELG SFHIRASYAA EERIEGRVAA
660 670 680 690 700
LQTAADAFYK AKNEFAAKAT EDQMRLLRIQ RRLEDELGGR FLDLSLHDTV
710 720 730 740 750
TTLILGGHNK RAEQLARDFR IPDKRLWWLK LAALADLEDW EELEKFSKSK
760 770 780 790 800
KSPIGYLPFV EICMKQHNKH EAKKYASRVG PEQKVKALLL VGDVAQAAEV
810 820 830
AIEHRNETEL SLVLSHCTGA TDGAIADKIQ RARAQAQKK
Length:839
Mass (Da):94,928
Last modified:July 27, 2011 - v3
Checksum:i42C9B3C87E5763C2
GO
Isoform 2 (identifier: Q920Q4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-420: Missing.

Note: No experimental confirmation available.
Show »
Length:419
Mass (Da):47,914
Checksum:iBA9044C076B695E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155L → Q in BAB64892 (Ref. 1) Curated1
Sequence conflicti173L → M in BAB64892 (Ref. 1) Curated1
Sequence conflicti173L → M in AAH25626 (PubMed:15489334).Curated1
Sequence conflicti431I → V in BAB64892 (Ref. 1) Curated1
Sequence conflicti493 – 499ARAWDMR → VQQKDVS in BAB64892 (Ref. 1) Curated7
Sequence conflicti493 – 499ARAWDMR → VQQKDVS in AAH03944 (PubMed:15489334).Curated7
Sequence conflicti493 – 499ARAWDMR → VQQKDVS in AAH25626 (PubMed:15489334).Curated7
Sequence conflicti653T → M in AAH25626 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0040191 – 420Missing in isoform 2. 1 PublicationAdd BLAST420

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056721 mRNA. Translation: BAB64892.1.
BX890605 Genomic DNA. Translation: CAM20123.1.
BC003944 mRNA. Translation: AAH03944.1.
BC025626 mRNA. Translation: AAH25626.1.
CCDSiCCDS38242.1. [Q920Q4-1]
UniGeneiMm.276092.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVPS16_MOUSE
AccessioniPrimary (citable) accession number: Q920Q4
Secondary accession number(s): A2BI91, Q8R3C3, Q99KZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 118 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families