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Q920Q2

- REV1_MOUSE

UniProt

Q920Q2 - REV1_MOUSE

Protein

DNA repair protein REV1

Gene

Rev1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei355 – 3551dCTPBy similarity
    Metal bindingi421 – 4211Magnesium 1PROSITE-ProRule annotation
    Metal bindingi421 – 4211Magnesium 2PROSITE-ProRule annotation
    Binding sitei520 – 5201dCTPBy similarity
    Metal bindingi568 – 5681Magnesium 1PROSITE-ProRule annotation
    Binding sitei568 – 5681dCTPBy similarity
    Metal bindingi569 – 5691Magnesium 1PROSITE-ProRule annotation
    Sitei768 – 7681Interaction with target DNABy similarity
    Sitei781 – 7811Interaction with target DNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi421 – 4255dCTP bindingBy similarity
    Nucleotide bindingi508 – 5147dCTP bindingBy similarity

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. deoxycytidyl transferase activity Source: MGI
    3. DNA-directed DNA polymerase activity Source: InterPro
    4. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. error-prone translesion synthesis Source: MGI
    2. response to UV Source: Ensembl

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA damage, DNA repair, DNA synthesis

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_216200. Translesion synthesis by HREV1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein REV1 (EC:2.7.7.-)
    Alternative name(s):
    Rev1-like terminal deoxycytidyl transferase
    Gene namesi
    Name:Rev1
    Synonyms:Rev1l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1929074. Rev1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12491249DNA repair protein REV1PRO_0000173993Add
    BLAST

    Proteomic databases

    PRIDEiQ920Q2.

    PTM databases

    PhosphoSiteiQ920Q2.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    ArrayExpressiQ920Q2.
    BgeeiQ920Q2.
    CleanExiMM_REV1.
    GenevestigatoriQ920Q2.

    Interactioni

    Subunit structurei

    Interacts with FAAP20 By similarity. Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi207845. 7 interactions.
    DIPiDIP-41772N.
    IntActiQ920Q2. 4 interactions.
    MINTiMINT-1031694.
    STRINGi10090.ENSMUSP00000027251.

    Structurei

    Secondary structure

    1
    1249
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1163 – 117614
    Helixi1182 – 119716
    Helixi1201 – 121717
    Helixi1221 – 124222
    Beta strandi1243 – 12464

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LSGNMR-A1150-1249[»]
    2LSJNMR-A1135-1249[»]
    4FJOX-ray2.72A1153-1249[»]
    ProteinModelPortaliQ920Q2.
    SMRiQ920Q2. Positions 48-133, 342-826, 1153-1249.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 13188BRCTPROSITE-ProRule annotationAdd
    BLAST
    Domaini417 – 651235UmuCPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni350 – 36011Interaction with target DNABy similarityAdd
    BLAST
    Regioni651 – 6544Interaction with target DNABy similarity
    Regioni707 – 7159Interaction with target DNABy similarity
    Regioni1150 – 1249100Protein interaction domain; mediates interaction with DNA polymerase zetaAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1072 – 10787Nuclear localization signalSequence Analysis

    Domaini

    The C-terminal domain is necessary for protein interactions.

    Sequence similaritiesi

    Belongs to the DNA polymerase type-Y family.Curated
    Contains 1 BRCT domain.PROSITE-ProRule annotation
    Contains 1 umuC domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0389.
    GeneTreeiENSGT00530000062942.
    HOGENOMiHOG000154094.
    HOVERGENiHBG054140.
    InParanoidiQ920Q2.
    KOiK03515.
    OMAiYRFCRGL.
    OrthoDBiEOG761BTT.
    PhylomeDBiQ920Q2.
    TreeFamiTF314488.

    Family and domain databases

    Gene3Di3.30.1490.100. 1 hit.
    3.40.50.10190. 1 hit.
    InterProiIPR001357. BRCT_dom.
    IPR017961. DNA_pol_Y-fam_little_finger.
    IPR001126. DNA_repair_prot_UmuC-like.
    IPR017963. DNA_repair_prot_UmuC-like_N.
    IPR012112. REV1.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF00817. IMS. 1 hit.
    PF11799. IMS_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036573. REV1. 1 hit.
    SMARTiSM00292. BRCT. 1 hit.
    [Graphical view]
    SUPFAMiSSF100879. SSF100879. 1 hit.
    SSF52113. SSF52113. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    PS50173. UMUC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q920Q2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRGGWRKRT ENDGWEKWGG YMAAKVQKLE EQFRTDAANQ KDGTASAIFS     50
    GVAIYVNGYT DPSAEELRNL MMLHGGQYHV YYSRSKTTHI IATNLPNAKI 100
    KELKGEKVIR PEWIVESIKA GRLLSSAPYQ LYTKPSAAQK SLNFNPVCKP 150
    EDPGPGPSNR AKQLNNRVNH IIKKIETESE VKANGLSSWN EDGVNDDFSF 200
    EDLEHTFPGR KQNGVMHPRD TAVIFNGHTH SSNGALKTQD CLVPVGNSVA 250
    SRLSLDSTQE EKRAEKSNAD FRDCTVQHLQ HSTRSADALR SPHRTNSLSP 300
    SLHSNTKING AHHSTVQGPS STKSTSVLTL SKVAPSVPSK PSDCNFISDF 350
    YSRSRLHHIS TWKCELTEFV NTLQRQSSGI FPGREKLKKV KTGRSSLVVT 400
    DTGTMSVLSS PRHQSCVMHV DMDCFFVSVG IRNRPDLKGK PVAVTSNRGT 450
    GTAPLRPGAN PQLEWQYYQN RALRGKAADI PDSSVWENQD STQTNGIDSV 500
    LSKAEIASCS YEARQVGIKN GMFFGYAKQL CPNLQAVPYD FHACREVAQA 550
    MYETLASYTH SIEAVSCDEA LIDVTDILAE TKLSPEEFAA ALRIEIKDKT 600
    KCAASVGIGS NILLARMATK KAKPDGQYHL QPDEVDDFIR GQLVTNLPGV 650
    GRSMESKLAS LGIKTCGDLQ CLTMAKLQKE FGPKTGQMLY RFCRGLDDRP 700
    VRTEKERKSV SAEINYGIRF TQPKEAEAFL LSLSEEIQRR LEAAGMKGKR 750
    LTLKIMVRKP GAPIETAKFG GHGICDNIAR TVTLDQATDS AKIIGKATLN 800
    MFHTMKLNIS DMRGVGIQVN QLVPANSNLS TCSSRPSAQS SLFSGRPHSV 850
    RDLFQLQKAK KPTEEEHKEV FLAAVDLEVS STSRACGLLS PLSAHLAASV 900
    SPDTNSGECS RKWNGLHSPV SGQSRLNLSI EVPSPSQIDQ SVLEALPLDL 950
    REQIEQVCAA QQGEPRGKKK EPVNGCSSGV LPHPVGTVLL QIPEPQEPCN 1000
    SDSKISVIAL PAFSQVDPDV FAALPAELQK ELKAAYDQRQ RQGEDTTHQQ 1050
    PTSTSVPKNP LLQLKPPAMK DKRNKRKNLI GSPRKSPLKN KLLSSPAKTL 1100
    PGAYGSPQKL MDGFLQHEGM ASERPLEEVS ASTPGAQDLS SLLPGQSSCF 1150
    RPAAPNLAGA VEFSDVKTLL KEWITTISDP MEEDILQVVR YCTDLIEEKD 1200
    LEKLDLVIKY MKRLMQQSVE SVWNMAFDFI LDNVQVVLQQ TYGSTLKVT 1249
    Length:1,249
    Mass (Da):137,341
    Last modified:December 1, 2001 - v1
    Checksum:iF82E0B6171AA3F75
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti438 – 4381K → T in AAF23323. 1 PublicationCurated
    Sequence conflicti555 – 5551L → W in AAF23323. 1 PublicationCurated
    Sequence conflicti638 – 6381F → S in AAF23323. 1 PublicationCurated
    Sequence conflicti661 – 6611L → S in AAF23323. 1 PublicationCurated
    Sequence conflicti684 – 6841K → R in AAF23323. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB057418 mRNA. Translation: BAB64933.1.
    AF179302 mRNA. Translation: AAF23323.1.
    BC058093 mRNA. Translation: AAH58093.1.
    CCDSiCCDS14899.1.
    RefSeqiNP_062516.2. NM_019570.3.
    XP_006496221.1. XM_006496158.1.
    XP_006496222.1. XM_006496159.1.
    XP_006496223.1. XM_006496160.1.
    UniGeneiMm.389103.

    Genome annotation databases

    EnsembliENSMUST00000027251; ENSMUSP00000027251; ENSMUSG00000026082.
    GeneIDi56210.
    KEGGimmu:56210.
    UCSCiuc007asp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB057418 mRNA. Translation: BAB64933.1 .
    AF179302 mRNA. Translation: AAF23323.1 .
    BC058093 mRNA. Translation: AAH58093.1 .
    CCDSi CCDS14899.1.
    RefSeqi NP_062516.2. NM_019570.3.
    XP_006496221.1. XM_006496158.1.
    XP_006496222.1. XM_006496159.1.
    XP_006496223.1. XM_006496160.1.
    UniGenei Mm.389103.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LSG NMR - A 1150-1249 [» ]
    2LSJ NMR - A 1135-1249 [» ]
    4FJO X-ray 2.72 A 1153-1249 [» ]
    ProteinModelPortali Q920Q2.
    SMRi Q920Q2. Positions 48-133, 342-826, 1153-1249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207845. 7 interactions.
    DIPi DIP-41772N.
    IntActi Q920Q2. 4 interactions.
    MINTi MINT-1031694.
    STRINGi 10090.ENSMUSP00000027251.

    PTM databases

    PhosphoSitei Q920Q2.

    Proteomic databases

    PRIDEi Q920Q2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027251 ; ENSMUSP00000027251 ; ENSMUSG00000026082 .
    GeneIDi 56210.
    KEGGi mmu:56210.
    UCSCi uc007asp.1. mouse.

    Organism-specific databases

    CTDi 51455.
    MGIi MGI:1929074. Rev1.

    Phylogenomic databases

    eggNOGi COG0389.
    GeneTreei ENSGT00530000062942.
    HOGENOMi HOG000154094.
    HOVERGENi HBG054140.
    InParanoidi Q920Q2.
    KOi K03515.
    OMAi YRFCRGL.
    OrthoDBi EOG761BTT.
    PhylomeDBi Q920Q2.
    TreeFami TF314488.

    Enzyme and pathway databases

    Reactomei REACT_216200. Translesion synthesis by HREV1.

    Miscellaneous databases

    ChiTaRSi REV1. mouse.
    NextBioi 312050.
    PROi Q920Q2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q920Q2.
    Bgeei Q920Q2.
    CleanExi MM_REV1.
    Genevestigatori Q920Q2.

    Family and domain databases

    Gene3Di 3.30.1490.100. 1 hit.
    3.40.50.10190. 1 hit.
    InterProi IPR001357. BRCT_dom.
    IPR017961. DNA_pol_Y-fam_little_finger.
    IPR001126. DNA_repair_prot_UmuC-like.
    IPR017963. DNA_repair_prot_UmuC-like_N.
    IPR012112. REV1.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF00817. IMS. 1 hit.
    PF11799. IMS_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036573. REV1. 1 hit.
    SMARTi SM00292. BRCT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF100879. SSF100879. 1 hit.
    SSF52113. SSF52113. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    PS50173. UMUC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mechanisms of dCMP transferase reactions catalyzed by mouse Rev1 protein."
      Masuda Y., Takahashi M., Fukuda S., Sumii M., Kamiya K.
      J. Biol. Chem. 277:3040-3046(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
      Strain: C3H/He.
      Tissue: Liver.
    2. "Mus musculus REV1 protein (Rev1) mRNA, complete cds."
      Poltoratsky V.P., Scharff M.D.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis."
      Guo C., Fischhaber P.L., Luk-Paszyc M.J., Masuda Y., Zhou J., Kamiya K., Kisker C., Friedberg E.C.
      EMBO J. 22:6621-6630(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAD2L2; POLH; POLI AND POLK.

    Entry informationi

    Entry nameiREV1_MOUSE
    AccessioniPrimary (citable) accession number: Q920Q2
    Secondary accession number(s): Q9QXV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3