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Q920Q2

- REV1_MOUSE

UniProt

Q920Q2 - REV1_MOUSE

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Protein
DNA repair protein REV1
Gene
Rev1, Rev1l
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei355 – 3551dCTP By similarity
Metal bindingi421 – 4211Magnesium 1 By similarity
Metal bindingi421 – 4211Magnesium 2 By similarity
Binding sitei520 – 5201dCTP By similarity
Metal bindingi568 – 5681Magnesium 1 By similarity
Binding sitei568 – 5681dCTP By similarity
Metal bindingi569 – 5691Magnesium 1 By similarity
Sitei768 – 7681Interaction with target DNA By similarity
Sitei781 – 7811Interaction with target DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi421 – 4255dCTP binding By similarity
Nucleotide bindingi508 – 5147dCTP binding By similarity

GO - Molecular functioni

  1. DNA-directed DNA polymerase activity Source: InterPro
  2. damaged DNA binding Source: InterPro
  3. deoxycytidyl transferase activity Source: MGI
  4. magnesium ion binding Source: InterPro

GO - Biological processi

  1. error-prone translesion synthesis Source: MGI
  2. response to UV Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_216200. Translesion synthesis by HREV1.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein REV1 (EC:2.7.7.-)
Alternative name(s):
Rev1-like terminal deoxycytidyl transferase
Gene namesi
Name:Rev1
Synonyms:Rev1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1929074. Rev1.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12491249DNA repair protein REV1
PRO_0000173993Add
BLAST

Proteomic databases

PRIDEiQ920Q2.

PTM databases

PhosphoSiteiQ920Q2.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ArrayExpressiQ920Q2.
BgeeiQ920Q2.
CleanExiMM_REV1.
GenevestigatoriQ920Q2.

Interactioni

Subunit structurei

Interacts with FAAP20 By similarity. Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK.2 Publications

Protein-protein interaction databases

BioGridi207845. 7 interactions.
DIPiDIP-41772N.
IntActiQ920Q2. 4 interactions.
MINTiMINT-1031694.
STRINGi10090.ENSMUSP00000027251.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1163 – 117614
Helixi1182 – 119716
Helixi1201 – 121717
Helixi1221 – 124222
Beta strandi1243 – 12464

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LSGNMR-A1150-1249[»]
2LSJNMR-A1135-1249[»]
4FJOX-ray2.72A1153-1249[»]
ProteinModelPortaliQ920Q2.
SMRiQ920Q2. Positions 48-133, 342-826, 1153-1249.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 13188BRCT
Add
BLAST
Domaini417 – 651235UmuC
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni350 – 36011Interaction with target DNA By similarity
Add
BLAST
Regioni651 – 6544Interaction with target DNA By similarity
Regioni707 – 7159Interaction with target DNA By similarity
Regioni1150 – 1249100Protein interaction domain; mediates interaction with DNA polymerase zeta
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1072 – 10787Nuclear localization signal Reviewed prediction

Domaini

The C-terminal domain is necessary for protein interactions.

Sequence similaritiesi

Contains 1 BRCT domain.
Contains 1 umuC domain.

Phylogenomic databases

eggNOGiCOG0389.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000154094.
HOVERGENiHBG054140.
InParanoidiQ920Q2.
KOiK03515.
OMAiYRFCRGL.
OrthoDBiEOG761BTT.
PhylomeDBiQ920Q2.
TreeFamiTF314488.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR012112. REV1.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF036573. REV1. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q920Q2-1 [UniParc]FASTAAdd to Basket

« Hide

MRRGGWRKRT ENDGWEKWGG YMAAKVQKLE EQFRTDAANQ KDGTASAIFS     50
GVAIYVNGYT DPSAEELRNL MMLHGGQYHV YYSRSKTTHI IATNLPNAKI 100
KELKGEKVIR PEWIVESIKA GRLLSSAPYQ LYTKPSAAQK SLNFNPVCKP 150
EDPGPGPSNR AKQLNNRVNH IIKKIETESE VKANGLSSWN EDGVNDDFSF 200
EDLEHTFPGR KQNGVMHPRD TAVIFNGHTH SSNGALKTQD CLVPVGNSVA 250
SRLSLDSTQE EKRAEKSNAD FRDCTVQHLQ HSTRSADALR SPHRTNSLSP 300
SLHSNTKING AHHSTVQGPS STKSTSVLTL SKVAPSVPSK PSDCNFISDF 350
YSRSRLHHIS TWKCELTEFV NTLQRQSSGI FPGREKLKKV KTGRSSLVVT 400
DTGTMSVLSS PRHQSCVMHV DMDCFFVSVG IRNRPDLKGK PVAVTSNRGT 450
GTAPLRPGAN PQLEWQYYQN RALRGKAADI PDSSVWENQD STQTNGIDSV 500
LSKAEIASCS YEARQVGIKN GMFFGYAKQL CPNLQAVPYD FHACREVAQA 550
MYETLASYTH SIEAVSCDEA LIDVTDILAE TKLSPEEFAA ALRIEIKDKT 600
KCAASVGIGS NILLARMATK KAKPDGQYHL QPDEVDDFIR GQLVTNLPGV 650
GRSMESKLAS LGIKTCGDLQ CLTMAKLQKE FGPKTGQMLY RFCRGLDDRP 700
VRTEKERKSV SAEINYGIRF TQPKEAEAFL LSLSEEIQRR LEAAGMKGKR 750
LTLKIMVRKP GAPIETAKFG GHGICDNIAR TVTLDQATDS AKIIGKATLN 800
MFHTMKLNIS DMRGVGIQVN QLVPANSNLS TCSSRPSAQS SLFSGRPHSV 850
RDLFQLQKAK KPTEEEHKEV FLAAVDLEVS STSRACGLLS PLSAHLAASV 900
SPDTNSGECS RKWNGLHSPV SGQSRLNLSI EVPSPSQIDQ SVLEALPLDL 950
REQIEQVCAA QQGEPRGKKK EPVNGCSSGV LPHPVGTVLL QIPEPQEPCN 1000
SDSKISVIAL PAFSQVDPDV FAALPAELQK ELKAAYDQRQ RQGEDTTHQQ 1050
PTSTSVPKNP LLQLKPPAMK DKRNKRKNLI GSPRKSPLKN KLLSSPAKTL 1100
PGAYGSPQKL MDGFLQHEGM ASERPLEEVS ASTPGAQDLS SLLPGQSSCF 1150
RPAAPNLAGA VEFSDVKTLL KEWITTISDP MEEDILQVVR YCTDLIEEKD 1200
LEKLDLVIKY MKRLMQQSVE SVWNMAFDFI LDNVQVVLQQ TYGSTLKVT 1249
Length:1,249
Mass (Da):137,341
Last modified:December 1, 2001 - v1
Checksum:iF82E0B6171AA3F75
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti438 – 4381K → T in AAF23323. 1 Publication
Sequence conflicti555 – 5551L → W in AAF23323. 1 Publication
Sequence conflicti638 – 6381F → S in AAF23323. 1 Publication
Sequence conflicti661 – 6611L → S in AAF23323. 1 Publication
Sequence conflicti684 – 6841K → R in AAF23323. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB057418 mRNA. Translation: BAB64933.1.
AF179302 mRNA. Translation: AAF23323.1.
BC058093 mRNA. Translation: AAH58093.1.
CCDSiCCDS14899.1.
RefSeqiNP_062516.2. NM_019570.3.
XP_006496221.1. XM_006496158.1.
XP_006496222.1. XM_006496159.1.
XP_006496223.1. XM_006496160.1.
UniGeneiMm.389103.

Genome annotation databases

EnsembliENSMUST00000027251; ENSMUSP00000027251; ENSMUSG00000026082.
GeneIDi56210.
KEGGimmu:56210.
UCSCiuc007asp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB057418 mRNA. Translation: BAB64933.1 .
AF179302 mRNA. Translation: AAF23323.1 .
BC058093 mRNA. Translation: AAH58093.1 .
CCDSi CCDS14899.1.
RefSeqi NP_062516.2. NM_019570.3.
XP_006496221.1. XM_006496158.1.
XP_006496222.1. XM_006496159.1.
XP_006496223.1. XM_006496160.1.
UniGenei Mm.389103.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LSG NMR - A 1150-1249 [» ]
2LSJ NMR - A 1135-1249 [» ]
4FJO X-ray 2.72 A 1153-1249 [» ]
ProteinModelPortali Q920Q2.
SMRi Q920Q2. Positions 48-133, 342-826, 1153-1249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207845. 7 interactions.
DIPi DIP-41772N.
IntActi Q920Q2. 4 interactions.
MINTi MINT-1031694.
STRINGi 10090.ENSMUSP00000027251.

PTM databases

PhosphoSitei Q920Q2.

Proteomic databases

PRIDEi Q920Q2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027251 ; ENSMUSP00000027251 ; ENSMUSG00000026082 .
GeneIDi 56210.
KEGGi mmu:56210.
UCSCi uc007asp.1. mouse.

Organism-specific databases

CTDi 51455.
MGIi MGI:1929074. Rev1.

Phylogenomic databases

eggNOGi COG0389.
GeneTreei ENSGT00530000062942.
HOGENOMi HOG000154094.
HOVERGENi HBG054140.
InParanoidi Q920Q2.
KOi K03515.
OMAi YRFCRGL.
OrthoDBi EOG761BTT.
PhylomeDBi Q920Q2.
TreeFami TF314488.

Enzyme and pathway databases

Reactomei REACT_216200. Translesion synthesis by HREV1.

Miscellaneous databases

ChiTaRSi REV1. mouse.
NextBioi 312050.
PROi Q920Q2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q920Q2.
Bgeei Q920Q2.
CleanExi MM_REV1.
Genevestigatori Q920Q2.

Family and domain databases

Gene3Di 3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProi IPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR012112. REV1.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF036573. REV1. 1 hit.
SMARTi SM00292. BRCT. 1 hit.
[Graphical view ]
SUPFAMi SSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mechanisms of dCMP transferase reactions catalyzed by mouse Rev1 protein."
    Masuda Y., Takahashi M., Fukuda S., Sumii M., Kamiya K.
    J. Biol. Chem. 277:3040-3046(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
    Strain: C3H/He.
    Tissue: Liver.
  2. "Mus musculus REV1 protein (Rev1) mRNA, complete cds."
    Poltoratsky V.P., Scharff M.D.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis."
    Guo C., Fischhaber P.L., Luk-Paszyc M.J., Masuda Y., Zhou J., Kamiya K., Kisker C., Friedberg E.C.
    EMBO J. 22:6621-6630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAD2L2; POLH; POLI AND POLK.

Entry informationi

Entry nameiREV1_MOUSE
AccessioniPrimary (citable) accession number: Q920Q2
Secondary accession number(s): Q9QXV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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