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Protein

DNA repair protein REV1

Gene

Rev1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei355dCTPBy similarity1
Metal bindingi421Magnesium 1PROSITE-ProRule annotation1
Metal bindingi421Magnesium 2PROSITE-ProRule annotation1
Binding sitei520dCTPBy similarity1
Metal bindingi568Magnesium 1PROSITE-ProRule annotation1
Binding sitei568dCTPBy similarity1
Metal bindingi569Magnesium 1PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi421 – 425dCTP bindingBy similarity5
Nucleotide bindingi508 – 514dCTP bindingBy similarity7

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • deoxycytidyl transferase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • error-prone translesion synthesis Source: MGI
  • response to UV Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA synthesis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-110312. Translesion synthesis by REV1.
R-MMU-5655862. Translesion synthesis by POLK.
R-MMU-5656121. Translesion synthesis by POLI.
R-MMU-5656169. Termination of translesion DNA synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein REV1 (EC:2.7.7.-)
Alternative name(s):
Rev1-like terminal deoxycytidyl transferase
Gene namesi
Name:Rev1
Synonyms:Rev1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1929074. Rev1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739931 – 1249DNA repair protein REV1Add BLAST1249

Proteomic databases

PaxDbiQ920Q2.
PeptideAtlasiQ920Q2.
PRIDEiQ920Q2.

PTM databases

iPTMnetiQ920Q2.
PhosphoSitePlusiQ920Q2.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000026082.
CleanExiMM_REV1.
ExpressionAtlasiQ920Q2. baseline and differential.
GenevisibleiQ920Q2. MM.

Interactioni

Subunit structurei

Interacts with FAAP20 (By similarity). Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei768Interaction with target DNABy similarity1
Sitei781Interaction with target DNABy similarity1

Protein-protein interaction databases

BioGridi207845. 10 interactors.
DIPiDIP-41772N.
IntActiQ920Q2. 4 interactors.
MINTiMINT-1031694.
STRINGi10090.ENSMUSP00000027251.

Structurei

Secondary structure

11249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1163 – 1176Combined sources14
Helixi1182 – 1197Combined sources16
Helixi1201 – 1217Combined sources17
Helixi1221 – 1242Combined sources22
Beta strandi1243 – 1246Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LSGNMR-A1150-1249[»]
2LSJNMR-A1135-1249[»]
4FJOX-ray2.72A1153-1249[»]
ProteinModelPortaliQ920Q2.
SMRiQ920Q2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 131BRCTPROSITE-ProRule annotationAdd BLAST88
Domaini417 – 651UmuCPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni350 – 360Interaction with target DNABy similarityAdd BLAST11
Regioni651 – 654Interaction with target DNABy similarity4
Regioni707 – 715Interaction with target DNABy similarity9
Regioni1150 – 1249Protein interaction domain; mediates interaction with DNA polymerase zetaAdd BLAST100

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1072 – 1078Nuclear localization signalSequence analysis7

Domaini

The C-terminal domain is necessary for protein interactions.

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2093. Eukaryota.
COG0389. LUCA.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000154094.
HOVERGENiHBG054140.
InParanoidiQ920Q2.
KOiK03515.
OMAiYRFCRGL.
OrthoDBiEOG091G02UI.
PhylomeDBiQ920Q2.
TreeFamiTF314488.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR025527. DUF4414.
IPR012112. REV1.
IPR031991. Rev1_C.
IPR001126. UmuC.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF14377. DUF4414. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF16727. REV1_C. 1 hit.
[Graphical view]
PIRSFiPIRSF036573. REV1. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q920Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRGGWRKRT ENDGWEKWGG YMAAKVQKLE EQFRTDAANQ KDGTASAIFS
60 70 80 90 100
GVAIYVNGYT DPSAEELRNL MMLHGGQYHV YYSRSKTTHI IATNLPNAKI
110 120 130 140 150
KELKGEKVIR PEWIVESIKA GRLLSSAPYQ LYTKPSAAQK SLNFNPVCKP
160 170 180 190 200
EDPGPGPSNR AKQLNNRVNH IIKKIETESE VKANGLSSWN EDGVNDDFSF
210 220 230 240 250
EDLEHTFPGR KQNGVMHPRD TAVIFNGHTH SSNGALKTQD CLVPVGNSVA
260 270 280 290 300
SRLSLDSTQE EKRAEKSNAD FRDCTVQHLQ HSTRSADALR SPHRTNSLSP
310 320 330 340 350
SLHSNTKING AHHSTVQGPS STKSTSVLTL SKVAPSVPSK PSDCNFISDF
360 370 380 390 400
YSRSRLHHIS TWKCELTEFV NTLQRQSSGI FPGREKLKKV KTGRSSLVVT
410 420 430 440 450
DTGTMSVLSS PRHQSCVMHV DMDCFFVSVG IRNRPDLKGK PVAVTSNRGT
460 470 480 490 500
GTAPLRPGAN PQLEWQYYQN RALRGKAADI PDSSVWENQD STQTNGIDSV
510 520 530 540 550
LSKAEIASCS YEARQVGIKN GMFFGYAKQL CPNLQAVPYD FHACREVAQA
560 570 580 590 600
MYETLASYTH SIEAVSCDEA LIDVTDILAE TKLSPEEFAA ALRIEIKDKT
610 620 630 640 650
KCAASVGIGS NILLARMATK KAKPDGQYHL QPDEVDDFIR GQLVTNLPGV
660 670 680 690 700
GRSMESKLAS LGIKTCGDLQ CLTMAKLQKE FGPKTGQMLY RFCRGLDDRP
710 720 730 740 750
VRTEKERKSV SAEINYGIRF TQPKEAEAFL LSLSEEIQRR LEAAGMKGKR
760 770 780 790 800
LTLKIMVRKP GAPIETAKFG GHGICDNIAR TVTLDQATDS AKIIGKATLN
810 820 830 840 850
MFHTMKLNIS DMRGVGIQVN QLVPANSNLS TCSSRPSAQS SLFSGRPHSV
860 870 880 890 900
RDLFQLQKAK KPTEEEHKEV FLAAVDLEVS STSRACGLLS PLSAHLAASV
910 920 930 940 950
SPDTNSGECS RKWNGLHSPV SGQSRLNLSI EVPSPSQIDQ SVLEALPLDL
960 970 980 990 1000
REQIEQVCAA QQGEPRGKKK EPVNGCSSGV LPHPVGTVLL QIPEPQEPCN
1010 1020 1030 1040 1050
SDSKISVIAL PAFSQVDPDV FAALPAELQK ELKAAYDQRQ RQGEDTTHQQ
1060 1070 1080 1090 1100
PTSTSVPKNP LLQLKPPAMK DKRNKRKNLI GSPRKSPLKN KLLSSPAKTL
1110 1120 1130 1140 1150
PGAYGSPQKL MDGFLQHEGM ASERPLEEVS ASTPGAQDLS SLLPGQSSCF
1160 1170 1180 1190 1200
RPAAPNLAGA VEFSDVKTLL KEWITTISDP MEEDILQVVR YCTDLIEEKD
1210 1220 1230 1240
LEKLDLVIKY MKRLMQQSVE SVWNMAFDFI LDNVQVVLQQ TYGSTLKVT
Length:1,249
Mass (Da):137,341
Last modified:December 1, 2001 - v1
Checksum:iF82E0B6171AA3F75
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti438K → T in AAF23323 (Ref. 2) Curated1
Sequence conflicti555L → W in AAF23323 (Ref. 2) Curated1
Sequence conflicti638F → S in AAF23323 (Ref. 2) Curated1
Sequence conflicti661L → S in AAF23323 (Ref. 2) Curated1
Sequence conflicti684K → R in AAF23323 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057418 mRNA. Translation: BAB64933.1.
AF179302 mRNA. Translation: AAF23323.1.
BC058093 mRNA. Translation: AAH58093.1.
CCDSiCCDS14899.1.
RefSeqiNP_062516.2. NM_019570.3.
XP_006496221.1. XM_006496158.2.
UniGeneiMm.389103.

Genome annotation databases

EnsembliENSMUST00000027251; ENSMUSP00000027251; ENSMUSG00000026082.
GeneIDi56210.
KEGGimmu:56210.
UCSCiuc007asp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057418 mRNA. Translation: BAB64933.1.
AF179302 mRNA. Translation: AAF23323.1.
BC058093 mRNA. Translation: AAH58093.1.
CCDSiCCDS14899.1.
RefSeqiNP_062516.2. NM_019570.3.
XP_006496221.1. XM_006496158.2.
UniGeneiMm.389103.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LSGNMR-A1150-1249[»]
2LSJNMR-A1135-1249[»]
4FJOX-ray2.72A1153-1249[»]
ProteinModelPortaliQ920Q2.
SMRiQ920Q2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207845. 10 interactors.
DIPiDIP-41772N.
IntActiQ920Q2. 4 interactors.
MINTiMINT-1031694.
STRINGi10090.ENSMUSP00000027251.

PTM databases

iPTMnetiQ920Q2.
PhosphoSitePlusiQ920Q2.

Proteomic databases

PaxDbiQ920Q2.
PeptideAtlasiQ920Q2.
PRIDEiQ920Q2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027251; ENSMUSP00000027251; ENSMUSG00000026082.
GeneIDi56210.
KEGGimmu:56210.
UCSCiuc007asp.1. mouse.

Organism-specific databases

CTDi51455.
MGIiMGI:1929074. Rev1.

Phylogenomic databases

eggNOGiKOG2093. Eukaryota.
COG0389. LUCA.
GeneTreeiENSGT00530000062942.
HOGENOMiHOG000154094.
HOVERGENiHBG054140.
InParanoidiQ920Q2.
KOiK03515.
OMAiYRFCRGL.
OrthoDBiEOG091G02UI.
PhylomeDBiQ920Q2.
TreeFamiTF314488.

Enzyme and pathway databases

ReactomeiR-MMU-110312. Translesion synthesis by REV1.
R-MMU-5655862. Translesion synthesis by POLK.
R-MMU-5656121. Translesion synthesis by POLI.
R-MMU-5656169. Termination of translesion DNA synthesis.

Miscellaneous databases

ChiTaRSiRev1. mouse.
PROiQ920Q2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026082.
CleanExiMM_REV1.
ExpressionAtlasiQ920Q2. baseline and differential.
GenevisibleiQ920Q2. MM.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.30.1490.100. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR025527. DUF4414.
IPR012112. REV1.
IPR031991. Rev1_C.
IPR001126. UmuC.
[Graphical view]
PfamiPF16589. BRCT_2. 1 hit.
PF14377. DUF4414. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF16727. REV1_C. 1 hit.
[Graphical view]
PIRSFiPIRSF036573. REV1. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiREV1_MOUSE
AccessioniPrimary (citable) accession number: Q920Q2
Secondary accession number(s): Q9QXV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.