DNA repair protein REV1 - Mus musculus (Mouse)

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Q920Q2 (REV1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA repair protein REV1
EC=2.7.7.-

Alternative name(s):
Rev1-like terminal deoxycytidyl transferase

Gene names

Name:	Rev1
Synonyms:	Rev1l

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Ref.1
Subunit structure	Interacts with FAAP20 By similarity. Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK. Ref.1 Ref.4
Subcellular location	Nucleus Probable.
Tissue specificity	Ubiquitous. Ref.1
Domain	The C-terminal domain is necessary for protein interactions.
Sequence similarities	Belongs to the DNA polymerase type-Y family. Contains 1 BRCT domain. Contains 1 umuC domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA synthesis
Cellular component	Nucleus
Ligand	DNA-binding Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone translesion synthesis Inferred from direct assay Ref.1. Source: MGI response to UV Inferred from electronic annotation. Source: Compara
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: InterPro damaged DNA binding Inferred from electronic annotation. Source: InterPro deoxycytidyl transferase activity Inferred from direct assay Ref.1. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1249

1249

DNA repair protein REV1

PRO_0000173993

Regions

Domain

44 – 131

BRCT

Domain

417 – 651

235

UmuC

Nucleotide binding

421 – 425

dCTP binding By similarity

Nucleotide binding

508 – 514

dCTP binding By similarity

Region

350 – 360

Interaction with target DNA By similarity

Region

651 – 654

Interaction with target DNA By similarity

Region

707 – 715

Interaction with target DNA By similarity

Region

1150 – 1249

100

Protein interaction domain; mediates interaction with DNA polymerase zeta

Motif

1072 – 1078

Nuclear localization signal Potential

Sites

Metal binding

421

Magnesium 1 By similarity

Metal binding

421

Magnesium 2 By similarity

Metal binding

568

Magnesium 1 By similarity

Metal binding

569

Magnesium 1 By similarity

Binding site

355

dCTP By similarity

Binding site

520

dCTP By similarity

Binding site

568

dCTP By similarity

Site

768

Interaction with target DNA By similarity

Site

781

Interaction with target DNA By similarity

Experimental info

Sequence conflict

438

K → T in AAF23323. Ref.2

Sequence conflict

555

L → W in AAF23323. Ref.2

Sequence conflict

638

F → S in AAF23323. Ref.2

Sequence conflict

661

L → S in AAF23323. Ref.2

Sequence conflict

684

K → R in AAF23323. Ref.2

Secondary structure

1249

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q920Q2 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: F82E0B6171AA3F75
Show »

FASTA

1,249

137,341

        10         20         30         40         50         60 
MRRGGWRKRT ENDGWEKWGG YMAAKVQKLE EQFRTDAANQ KDGTASAIFS GVAIYVNGYT 

        70         80         90        100        110        120 
DPSAEELRNL MMLHGGQYHV YYSRSKTTHI IATNLPNAKI KELKGEKVIR PEWIVESIKA 

       130        140        150        160        170        180 
GRLLSSAPYQ LYTKPSAAQK SLNFNPVCKP EDPGPGPSNR AKQLNNRVNH IIKKIETESE 

       190        200        210        220        230        240 
VKANGLSSWN EDGVNDDFSF EDLEHTFPGR KQNGVMHPRD TAVIFNGHTH SSNGALKTQD 

       250        260        270        280        290        300 
CLVPVGNSVA SRLSLDSTQE EKRAEKSNAD FRDCTVQHLQ HSTRSADALR SPHRTNSLSP 

       310        320        330        340        350        360 
SLHSNTKING AHHSTVQGPS STKSTSVLTL SKVAPSVPSK PSDCNFISDF YSRSRLHHIS 

       370        380        390        400        410        420 
TWKCELTEFV NTLQRQSSGI FPGREKLKKV KTGRSSLVVT DTGTMSVLSS PRHQSCVMHV 

       430        440        450        460        470        480 
DMDCFFVSVG IRNRPDLKGK PVAVTSNRGT GTAPLRPGAN PQLEWQYYQN RALRGKAADI 

       490        500        510        520        530        540 
PDSSVWENQD STQTNGIDSV LSKAEIASCS YEARQVGIKN GMFFGYAKQL CPNLQAVPYD 

       550        560        570        580        590        600 
FHACREVAQA MYETLASYTH SIEAVSCDEA LIDVTDILAE TKLSPEEFAA ALRIEIKDKT 

       610        620        630        640        650        660 
KCAASVGIGS NILLARMATK KAKPDGQYHL QPDEVDDFIR GQLVTNLPGV GRSMESKLAS 

       670        680        690        700        710        720 
LGIKTCGDLQ CLTMAKLQKE FGPKTGQMLY RFCRGLDDRP VRTEKERKSV SAEINYGIRF 

       730        740        750        760        770        780 
TQPKEAEAFL LSLSEEIQRR LEAAGMKGKR LTLKIMVRKP GAPIETAKFG GHGICDNIAR 

       790        800        810        820        830        840 
TVTLDQATDS AKIIGKATLN MFHTMKLNIS DMRGVGIQVN QLVPANSNLS TCSSRPSAQS 

       850        860        870        880        890        900 
SLFSGRPHSV RDLFQLQKAK KPTEEEHKEV FLAAVDLEVS STSRACGLLS PLSAHLAASV 

       910        920        930        940        950        960 
SPDTNSGECS RKWNGLHSPV SGQSRLNLSI EVPSPSQIDQ SVLEALPLDL REQIEQVCAA 

       970        980        990       1000       1010       1020 
QQGEPRGKKK EPVNGCSSGV LPHPVGTVLL QIPEPQEPCN SDSKISVIAL PAFSQVDPDV 

      1030       1040       1050       1060       1070       1080 
FAALPAELQK ELKAAYDQRQ RQGEDTTHQQ PTSTSVPKNP LLQLKPPAMK DKRNKRKNLI 

      1090       1100       1110       1120       1130       1140 
GSPRKSPLKN KLLSSPAKTL PGAYGSPQKL MDGFLQHEGM ASERPLEEVS ASTPGAQDLS 

      1150       1160       1170       1180       1190       1200 
SLLPGQSSCF RPAAPNLAGA VEFSDVKTLL KEWITTISDP MEEDILQVVR YCTDLIEEKD 

      1210       1220       1230       1240 
LEKLDLVIKY MKRLMQQSVE SVWNMAFDFI LDNVQVVLQQ TYGSTLKVT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mechanisms of dCMP transferase reactions catalyzed by mouse Rev1 protein." Masuda Y., Takahashi M., Fukuda S., Sumii M., Kamiya K. J. Biol. Chem. 277:3040-3046(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, TISSUE SPECIFICITY. Strain: C3H/He. Tissue: Liver.
[2]	"Mus musculus REV1 protein (Rev1) mRNA, complete cds." Poltoratsky V.P., Scharff M.D. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis." Guo C., Fischhaber P.L., Luk-Paszyc M.J., Masuda Y., Zhou J., Kamiya K., Kisker C., Friedberg E.C. EMBO J. 22:6621-6630(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAD2L2; POLH; POLI AND POLK.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB057418 mRNA. Translation: BAB64933.1.
AF179302 mRNA. Translation: AAF23323.1.
BC058093 mRNA. Translation: AAH58093.1.

IPI

IPI00396706.

RefSeq

NP_062516.2. NM_019570.3.

UniGene

Mm.389103.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LSG	NMR	-	A	1150-1249	[»]
2LSJ	NMR	-	A	1135-1249	[»]
4FJO	X-ray	2.72	A	1153-1249	[»]

ProteinModelPortal

Q920Q2.

SMR

Q920Q2. Positions 48-133, 342-826, 1153-1249.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-41772N.

IntAct

Q920Q2. 4 interactions.

STRING

10090.ENSMUSP00000027251.

PTM databases

PhosphoSite

Q920Q2.

Proteomic databases

PRIDE

Q920Q2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000027251; ENSMUSP00000027251; ENSMUSG00000026082.

GeneID

56210.

KEGG

mmu:56210.

UCSC

uc007asp.1. mouse.

Organism-specific databases

CTD

51455.

MGI

MGI:1929074. Rev1.

Phylogenomic databases

eggNOG

COG0389.

GeneTree

ENSGT00530000062942.

HOGENOM

HOG000154094.

HOVERGEN

HBG054140.

InParanoid

Q920Q2.

K03515.

OMA

YRFCRGL.

OrthoDB

EOG4GF3DF.

Gene expression databases

ArrayExpress

Q920Q2.

Bgee

Q920Q2.

CleanEx

MM_REV1.

Genevestigator

Q920Q2.

GermOnline

ENSMUSG00000026082. Mus musculus.

Family and domain databases

Gene3D

3.30.1490.100. 1 hit.

InterPro

IPR001357. BRCT_dom.
IPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR012112. REV1.
[Graphical view]

Pfam

PF00533. BRCT. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]

PIRSF

PIRSF036573. REV1. 1 hit.

SMART

SM00292. BRCT. 1 hit.
[Graphical view]

SUPFAM

SSF52113. BRCT. 1 hit.
SSF100879. DNA_pol_Y-fam_little_finger. 1 hit.

PROSITE

PS50172. BRCT. 1 hit.
PS50173. UMUC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

REV1. mouse.

NextBio

312050.

SOURCE

Search...

Entry information

Entry name

REV1_MOUSE

Accession

Primary (citable) accession number: Q920Q2
Secondary accession number(s): Q9QXV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents