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Q920P6

- ADA_RAT

UniProt

Q920P6 - ADA_RAT

Protein

Adenosine deaminase

Gene

Ada

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion By similarity.By similarity

    Catalytic activityi

    Adenosine + H2O = inosine + NH3.1 Publication

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi15 – 151Zinc; catalyticBy similarity
    Metal bindingi17 – 171Zinc; catalyticBy similarity
    Binding sitei17 – 171SubstrateBy similarity
    Binding sitei19 – 191SubstrateBy similarity
    Binding sitei184 – 1841Substrate; via amide nitrogen and carbonyl oxygenBy similarity
    Metal bindingi214 – 2141Zinc; catalyticBy similarity
    Active sitei217 – 2171Proton donorBy similarity
    Sitei238 – 2381Important for catalytic activityBy similarity
    Metal bindingi295 – 2951Zinc; catalyticBy similarity
    Binding sitei296 – 2961SubstrateBy similarity

    GO - Molecular functioni

    1. adenosine deaminase activity Source: UniProtKB
    2. purine nucleoside binding Source: RGD
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. adenosine catabolic process Source: UniProtKB
    2. adenosine metabolic process Source: RGD
    3. aging Source: RGD
    4. cell adhesion Source: UniProtKB-KW
    5. dATP catabolic process Source: Ensembl
    6. deoxyadenosine catabolic process Source: Ensembl
    7. embryonic digestive tract development Source: Ensembl
    8. germinal center B cell differentiation Source: Ensembl
    9. histamine secretion Source: RGD
    10. hypoxanthine salvage Source: RefGenome
    11. inosine biosynthetic process Source: UniProtKB
    12. liver development Source: Ensembl
    13. lung alveolus development Source: Ensembl
    14. negative regulation of adenosine receptor signaling pathway Source: RefGenome
    15. negative regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
    16. negative regulation of inflammatory response Source: Ensembl
    17. negative regulation of leukocyte migration Source: Ensembl
    18. negative regulation of mature B cell apoptotic process Source: Ensembl
    19. negative regulation of mucus secretion Source: Ensembl
    20. negative regulation of penile erection Source: Ensembl
    21. negative regulation of thymocyte apoptotic process Source: Ensembl
    22. Peyer's patch development Source: Ensembl
    23. placenta development Source: Ensembl
    24. positive regulation of alpha-beta T cell differentiation Source: Ensembl
    25. positive regulation of B cell proliferation Source: Ensembl
    26. positive regulation of calcium-mediated signaling Source: Ensembl
    27. positive regulation of germinal center formation Source: Ensembl
    28. positive regulation of heart rate Source: Ensembl
    29. positive regulation of smooth muscle contraction Source: Ensembl
    30. positive regulation of T cell differentiation in thymus Source: Ensembl
    31. positive regulation of T cell receptor signaling pathway Source: Ensembl
    32. purine nucleotide salvage Source: Ensembl
    33. purine ribonucleoside monophosphate biosynthetic process Source: InterPro
    34. regulation of cell-cell adhesion mediated by integrin Source: Ensembl
    35. regulation of circadian sleep/wake cycle, sleep Source: RGD
    36. response to drug Source: RGD
    37. response to hydrogen peroxide Source: RGD
    38. response to hypoxia Source: RGD
    39. response to morphine Source: RGD
    40. response to vitamin E Source: RGD
    41. T cell activation Source: RefGenome
    42. trophectodermal cell differentiation Source: Ensembl
    43. xanthine biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell adhesion, Nucleotide metabolism

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_221255. Purine salvage.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosine deaminase (EC:3.5.4.4)
    Alternative name(s):
    Adenosine aminohydrolase
    Gene namesi
    Name:Ada
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi2031. Ada.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity; Extracellular side By similarity. Cell junction By similarity. Cytoplasmic vesicle lumen 2 Publications. Cytoplasm By similarity
    Note: Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-SubCell
    2. cytoplasm Source: RGD
    3. cytoplasmic membrane-bounded vesicle lumen Source: UniProtKB-SubCell
    4. cytosol Source: RefGenome
    5. dendrite cytoplasm Source: RGD
    6. external side of plasma membrane Source: RefGenome
    7. extracellular space Source: RGD
    8. lysosome Source: Ensembl
    9. neuronal cell body Source: RGD

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 352351Adenosine deaminasePRO_0000194354Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei54 – 541N6-acetyllysineBy similarity
    Modified residuei232 – 2321N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ920P6.
    PRIDEiQ920P6.

    PTM databases

    PhosphoSiteiQ920P6.

    Expressioni

    Tissue specificityi

    Detected in brain and liver (at protein level).2 Publications

    Gene expression databases

    GenevestigatoriQ920P6.

    Interactioni

    Subunit structurei

    Interacts with DPP4 (extracellular domain).By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000014151.

    Structurei

    3D structure databases

    ProteinModelPortaliQ920P6.
    SMRiQ920P6. Positions 4-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1816.
    GeneTreeiENSGT00730000111151.
    HOGENOMiHOG000218816.
    HOVERGENiHBG001718.
    InParanoidiQ920P6.
    KOiK01488.
    OMAiELHCHLF.
    OrthoDBiEOG7GN2MZ.
    PhylomeDBiQ920P6.
    TreeFamiTF314270.

    Family and domain databases

    HAMAPiMF_00540. A_deaminase.
    InterProiIPR006650. A/AMP_deam_AS.
    IPR001365. A/AMP_deaminase_dom.
    IPR028893. A_deaminase.
    IPR006330. Ado/ade_deaminase.
    [Graphical view]
    PfamiPF00962. A_deaminase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01430. aden_deam. 1 hit.
    PROSITEiPS00485. A_DEAMINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q920P6-1 [UniParc]FASTAAdd to Basket

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    MAQTPAFNKP KVELHVHLDG AIKPETILYY GKKRGIDLPA DTVEGLRNII    50
    GMDKPLSLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV 100
    RYSPHLLANS KVDPIPWNQA EGDLTPDEVV DLVNQGLQEG EQAFGIKVRS 150
    ILCCMRHQPS WSPEVLELCK KYHQKTVVAM DLAGDETIEG SSLFPGHVEA 200
    YEGAVKDGIH RTVHAGEVGS AEVVREAVDI LKTERVGHGY HTIEDEALYN 250
    RLLKENMHFE VCPWSSYLTG AWNPKTTHAV VRFKDDQANY SLNSDDPLIF 300
    KSTVDTDYQM VKKDMGFTEE EFKRLNINAA KSSFLPEDEK KELLERLYKE 350
    YQ 352
    Length:352
    Mass (Da):39,899
    Last modified:January 23, 2007 - v3
    Checksum:iF689ADFD39682FC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059655 mRNA. Translation: BAB69691.1.
    BC088116 mRNA. Translation: AAH88116.1.
    RefSeqiNP_569083.1. NM_130399.2.
    UniGeneiRn.12689.

    Genome annotation databases

    EnsembliENSRNOT00000014151; ENSRNOP00000014151; ENSRNOG00000010265.
    GeneIDi24165.
    KEGGirno:24165.
    UCSCiRGD:2031. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB059655 mRNA. Translation: BAB69691.1 .
    BC088116 mRNA. Translation: AAH88116.1 .
    RefSeqi NP_569083.1. NM_130399.2.
    UniGenei Rn.12689.

    3D structure databases

    ProteinModelPortali Q920P6.
    SMRi Q920P6. Positions 4-352.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000014151.

    PTM databases

    PhosphoSitei Q920P6.

    Proteomic databases

    PaxDbi Q920P6.
    PRIDEi Q920P6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000014151 ; ENSRNOP00000014151 ; ENSRNOG00000010265 .
    GeneIDi 24165.
    KEGGi rno:24165.
    UCSCi RGD:2031. rat.

    Organism-specific databases

    CTDi 100.
    RGDi 2031. Ada.

    Phylogenomic databases

    eggNOGi COG1816.
    GeneTreei ENSGT00730000111151.
    HOGENOMi HOG000218816.
    HOVERGENi HBG001718.
    InParanoidi Q920P6.
    KOi K01488.
    OMAi ELHCHLF.
    OrthoDBi EOG7GN2MZ.
    PhylomeDBi Q920P6.
    TreeFami TF314270.

    Enzyme and pathway databases

    Reactomei REACT_221255. Purine salvage.

    Miscellaneous databases

    NextBioi 602469.
    PROi Q920P6.

    Gene expression databases

    Genevestigatori Q920P6.

    Family and domain databases

    HAMAPi MF_00540. A_deaminase.
    InterProi IPR006650. A/AMP_deam_AS.
    IPR001365. A/AMP_deaminase_dom.
    IPR028893. A_deaminase.
    IPR006330. Ado/ade_deaminase.
    [Graphical view ]
    Pfami PF00962. A_deaminase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01430. aden_deam. 1 hit.
    PROSITEi PS00485. A_DEAMINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Restricted expression of adenosine deaminase in rat leptomeninges and its role in the regulation of sleep."
      Okada T., Mochizuki T., Huag Z., Sugita Y., Urade Y., Hayaishi O.
      Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Leptomeninges.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    3. "Adenosine deaminase in rodent median eminence: detection by antibody to the mouse enzyme and co-localization with adenosine deaminase-complexing protein (CD26)."
      Nagy J.I., Yamamoto T., Uemura H., Schrader W.P.
      Neuroscience 73:459-471(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    4. "AMP deaminase and adenosine deaminase activities in liver and brain regions in acute ammonia intoxication and subacute toxic hepatitis."
      Kaminsky Y., Kosenko E.
      Brain Res. 1311:175-181(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiADA_RAT
    AccessioniPrimary (citable) accession number: Q920P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3