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Q920P6

- ADA_RAT

UniProt

Q920P6 - ADA_RAT

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Protein

Adenosine deaminase

Gene

Ada

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity).By similarity

Catalytic activityi

Adenosine + H2O = inosine + NH3.1 Publication

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi15 – 151Zinc; catalyticBy similarity
Metal bindingi17 – 171Zinc; catalyticBy similarity
Binding sitei17 – 171SubstrateBy similarity
Binding sitei19 – 191SubstrateBy similarity
Binding sitei184 – 1841Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Metal bindingi214 – 2141Zinc; catalyticBy similarity
Active sitei217 – 2171Proton donorBy similarity
Sitei238 – 2381Important for catalytic activityBy similarity
Metal bindingi295 – 2951Zinc; catalyticBy similarity
Binding sitei296 – 2961SubstrateBy similarity

GO - Molecular functioni

  1. adenosine deaminase activity Source: UniProtKB
  2. purine nucleoside binding Source: RGD
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. adenosine catabolic process Source: UniProtKB
  2. adenosine metabolic process Source: RGD
  3. aging Source: RGD
  4. cell adhesion Source: UniProtKB-KW
  5. dATP catabolic process Source: Ensembl
  6. deoxyadenosine catabolic process Source: Ensembl
  7. embryonic digestive tract development Source: Ensembl
  8. germinal center B cell differentiation Source: Ensembl
  9. histamine secretion Source: RGD
  10. hypoxanthine salvage Source: RefGenome
  11. inosine biosynthetic process Source: UniProtKB
  12. liver development Source: Ensembl
  13. lung alveolus development Source: Ensembl
  14. negative regulation of adenosine receptor signaling pathway Source: RefGenome
  15. negative regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
  16. negative regulation of inflammatory response Source: Ensembl
  17. negative regulation of leukocyte migration Source: Ensembl
  18. negative regulation of mature B cell apoptotic process Source: Ensembl
  19. negative regulation of mucus secretion Source: Ensembl
  20. negative regulation of penile erection Source: Ensembl
  21. negative regulation of thymocyte apoptotic process Source: Ensembl
  22. Peyer's patch development Source: Ensembl
  23. placenta development Source: Ensembl
  24. positive regulation of alpha-beta T cell differentiation Source: Ensembl
  25. positive regulation of B cell proliferation Source: Ensembl
  26. positive regulation of calcium-mediated signaling Source: Ensembl
  27. positive regulation of germinal center formation Source: Ensembl
  28. positive regulation of heart rate Source: Ensembl
  29. positive regulation of smooth muscle contraction Source: Ensembl
  30. positive regulation of T cell differentiation in thymus Source: Ensembl
  31. positive regulation of T cell receptor signaling pathway Source: Ensembl
  32. purine nucleotide salvage Source: Ensembl
  33. purine ribonucleoside monophosphate biosynthetic process Source: InterPro
  34. regulation of cell-cell adhesion mediated by integrin Source: Ensembl
  35. regulation of circadian sleep/wake cycle, sleep Source: RGD
  36. response to drug Source: RGD
  37. response to hydrogen peroxide Source: RGD
  38. response to hypoxia Source: RGD
  39. response to morphine Source: RGD
  40. response to vitamin E Source: RGD
  41. T cell activation Source: RefGenome
  42. trophectodermal cell differentiation Source: Ensembl
  43. xanthine biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell adhesion, Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_221255. Purine salvage.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine deaminase (EC:3.5.4.4)
Alternative name(s):
Adenosine aminohydrolase
Gene namesi
Name:Ada
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi2031. Ada.

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity; Extracellular side By similarity. Cell junction By similarity. Cytoplasmic vesicle lumen 2 Publications. Cytoplasm By similarity
Note: Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytoplasm Source: RGD
  3. cytoplasmic vesicle Source: UniProtKB-KW
  4. cytosol Source: RefGenome
  5. dendrite cytoplasm Source: RGD
  6. external side of plasma membrane Source: RefGenome
  7. extracellular space Source: RGD
  8. lysosome Source: Ensembl
  9. neuronal cell body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 352351Adenosine deaminasePRO_0000194354Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei54 – 541N6-acetyllysineBy similarity
Modified residuei232 – 2321N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ920P6.
PRIDEiQ920P6.

PTM databases

PhosphoSiteiQ920P6.

Expressioni

Tissue specificityi

Detected in brain and liver (at protein level).2 Publications

Gene expression databases

GenevestigatoriQ920P6.

Interactioni

Subunit structurei

Interacts with DPP4 (extracellular domain).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014151.

Structurei

3D structure databases

ProteinModelPortaliQ920P6.
SMRiQ920P6. Positions 4-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1816.
GeneTreeiENSGT00730000111151.
HOGENOMiHOG000218816.
HOVERGENiHBG001718.
InParanoidiQ920P6.
KOiK01488.
OMAiELHCHLF.
OrthoDBiEOG7GN2MZ.
PhylomeDBiQ920P6.
TreeFamiTF314270.

Family and domain databases

HAMAPiMF_00540. A_deaminase.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028893. A_deaminase.
IPR006330. Ado/ade_deaminase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01430. aden_deam. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q920P6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQTPAFNKP KVELHVHLDG AIKPETILYY GKKRGIDLPA DTVEGLRNII
60 70 80 90 100
GMDKPLSLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV
110 120 130 140 150
RYSPHLLANS KVDPIPWNQA EGDLTPDEVV DLVNQGLQEG EQAFGIKVRS
160 170 180 190 200
ILCCMRHQPS WSPEVLELCK KYHQKTVVAM DLAGDETIEG SSLFPGHVEA
210 220 230 240 250
YEGAVKDGIH RTVHAGEVGS AEVVREAVDI LKTERVGHGY HTIEDEALYN
260 270 280 290 300
RLLKENMHFE VCPWSSYLTG AWNPKTTHAV VRFKDDQANY SLNSDDPLIF
310 320 330 340 350
KSTVDTDYQM VKKDMGFTEE EFKRLNINAA KSSFLPEDEK KELLERLYKE

YQ
Length:352
Mass (Da):39,899
Last modified:January 23, 2007 - v3
Checksum:iF689ADFD39682FC6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059655 mRNA. Translation: BAB69691.1.
BC088116 mRNA. Translation: AAH88116.1.
RefSeqiNP_569083.1. NM_130399.2.
UniGeneiRn.12689.

Genome annotation databases

EnsembliENSRNOT00000014151; ENSRNOP00000014151; ENSRNOG00000010265.
GeneIDi24165.
KEGGirno:24165.
UCSCiRGD:2031. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB059655 mRNA. Translation: BAB69691.1 .
BC088116 mRNA. Translation: AAH88116.1 .
RefSeqi NP_569083.1. NM_130399.2.
UniGenei Rn.12689.

3D structure databases

ProteinModelPortali Q920P6.
SMRi Q920P6. Positions 4-352.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000014151.

PTM databases

PhosphoSitei Q920P6.

Proteomic databases

PaxDbi Q920P6.
PRIDEi Q920P6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000014151 ; ENSRNOP00000014151 ; ENSRNOG00000010265 .
GeneIDi 24165.
KEGGi rno:24165.
UCSCi RGD:2031. rat.

Organism-specific databases

CTDi 100.
RGDi 2031. Ada.

Phylogenomic databases

eggNOGi COG1816.
GeneTreei ENSGT00730000111151.
HOGENOMi HOG000218816.
HOVERGENi HBG001718.
InParanoidi Q920P6.
KOi K01488.
OMAi ELHCHLF.
OrthoDBi EOG7GN2MZ.
PhylomeDBi Q920P6.
TreeFami TF314270.

Enzyme and pathway databases

Reactomei REACT_221255. Purine salvage.

Miscellaneous databases

NextBioi 602469.
PROi Q920P6.

Gene expression databases

Genevestigatori Q920P6.

Family and domain databases

HAMAPi MF_00540. A_deaminase.
InterProi IPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028893. A_deaminase.
IPR006330. Ado/ade_deaminase.
[Graphical view ]
Pfami PF00962. A_deaminase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01430. aden_deam. 1 hit.
PROSITEi PS00485. A_DEAMINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Restricted expression of adenosine deaminase in rat leptomeninges and its role in the regulation of sleep."
    Okada T., Mochizuki T., Huag Z., Sugita Y., Urade Y., Hayaishi O.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Leptomeninges.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  3. "Adenosine deaminase in rodent median eminence: detection by antibody to the mouse enzyme and co-localization with adenosine deaminase-complexing protein (CD26)."
    Nagy J.I., Yamamoto T., Uemura H., Schrader W.P.
    Neuroscience 73:459-471(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "AMP deaminase and adenosine deaminase activities in liver and brain regions in acute ammonia intoxication and subacute toxic hepatitis."
    Kaminsky Y., Kosenko E.
    Brain Res. 1311:175-181(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiADA_RAT
AccessioniPrimary (citable) accession number: Q920P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3