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Protein

Adenosine deaminase

Gene

Ada

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion. Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development.By similarity

Catalytic activityi

Adenosine + H2O = inosine + NH3.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Zinc; catalyticBy similarity1
Metal bindingi17Zinc; catalyticBy similarity1
Binding sitei17SubstrateBy similarity1
Binding sitei19SubstrateBy similarity1
Sitei58Important for interaction with adenosine receptors and increasing their affinity for agonistsBy similarity1
Sitei62Important for interaction with adenosine receptors and increasing their affinity for agonistsBy similarity1
Binding sitei184Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi214Zinc; catalyticBy similarity1
Active sitei217Proton donorBy similarity1
Sitei238Important for catalytic activityBy similarity1
Metal bindingi295Zinc; catalyticBy similarity1
Binding sitei296SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell adhesion, Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-74217. Purine salvage.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine deaminase (EC:3.5.4.41 Publication)
Alternative name(s):
Adenosine aminohydrolase
Gene namesi
Name:Ada
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2031. Ada.

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity; Extracellular side By similarity
  • Cell junction By similarity
  • Cytoplasmic vesicle lumen 2 Publications
  • Cytoplasm By similarity
  • Lysosome By similarity

  • Note: Colocalized with DPP4 at the cell surface.By similarity

GO - Cellular componenti

  • cell junction Source: UniProtKB-SubCell
  • cytoplasm Source: RGD
  • cytoplasmic membrane-bounded vesicle lumen Source: UniProtKB-SubCell
  • cytosol Source: GO_Central
  • dendrite cytoplasm Source: RGD
  • external side of plasma membrane Source: GO_Central
  • extracellular space Source: RGD
  • lysosome Source: Ensembl
  • neuronal cell body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001943542 – 352Adenosine deaminaseAdd BLAST351

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei54N6-acetyllysineBy similarity1
Modified residuei232N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ920P6.
PRIDEiQ920P6.

PTM databases

iPTMnetiQ920P6.
PhosphoSitePlusiQ920P6.

Expressioni

Tissue specificityi

Detected in brain and liver (at protein level).2 Publications

Gene expression databases

BgeeiENSRNOG00000010265.
GenevisibleiQ920P6. RN.

Interactioni

Subunit structurei

Interacts with DPP4 (via extracellular domain). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014151.

Structurei

3D structure databases

ProteinModelPortaliQ920P6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1097. Eukaryota.
COG1816. LUCA.
GeneTreeiENSGT00730000111151.
HOGENOMiHOG000218816.
HOVERGENiHBG001718.
InParanoidiQ920P6.
KOiK01488.
OMAiMPAIAGC.
OrthoDBiEOG091G0NL8.
PhylomeDBiQ920P6.
TreeFamiTF314270.

Family and domain databases

CDDicd01320. ADA. 1 hit.
HAMAPiMF_00540. A_deaminase. 1 hit.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028893. A_deaminase.
IPR006330. Ado/ade_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01430. aden_deam. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q920P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQTPAFNKP KVELHVHLDG AIKPETILYY GKKRGIDLPA DTVEGLRNII
60 70 80 90 100
GMDKPLSLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV
110 120 130 140 150
RYSPHLLANS KVDPIPWNQA EGDLTPDEVV DLVNQGLQEG EQAFGIKVRS
160 170 180 190 200
ILCCMRHQPS WSPEVLELCK KYHQKTVVAM DLAGDETIEG SSLFPGHVEA
210 220 230 240 250
YEGAVKDGIH RTVHAGEVGS AEVVREAVDI LKTERVGHGY HTIEDEALYN
260 270 280 290 300
RLLKENMHFE VCPWSSYLTG AWNPKTTHAV VRFKDDQANY SLNSDDPLIF
310 320 330 340 350
KSTVDTDYQM VKKDMGFTEE EFKRLNINAA KSSFLPEDEK KELLERLYKE

YQ
Length:352
Mass (Da):39,899
Last modified:January 23, 2007 - v3
Checksum:iF689ADFD39682FC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059655 mRNA. Translation: BAB69691.1.
BC088116 mRNA. Translation: AAH88116.1.
RefSeqiNP_569083.1. NM_130399.2.
UniGeneiRn.12689.

Genome annotation databases

EnsembliENSRNOT00000014151; ENSRNOP00000014151; ENSRNOG00000010265.
GeneIDi24165.
KEGGirno:24165.
UCSCiRGD:2031. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059655 mRNA. Translation: BAB69691.1.
BC088116 mRNA. Translation: AAH88116.1.
RefSeqiNP_569083.1. NM_130399.2.
UniGeneiRn.12689.

3D structure databases

ProteinModelPortaliQ920P6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014151.

PTM databases

iPTMnetiQ920P6.
PhosphoSitePlusiQ920P6.

Proteomic databases

PaxDbiQ920P6.
PRIDEiQ920P6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014151; ENSRNOP00000014151; ENSRNOG00000010265.
GeneIDi24165.
KEGGirno:24165.
UCSCiRGD:2031. rat.

Organism-specific databases

CTDi100.
RGDi2031. Ada.

Phylogenomic databases

eggNOGiKOG1097. Eukaryota.
COG1816. LUCA.
GeneTreeiENSGT00730000111151.
HOGENOMiHOG000218816.
HOVERGENiHBG001718.
InParanoidiQ920P6.
KOiK01488.
OMAiMPAIAGC.
OrthoDBiEOG091G0NL8.
PhylomeDBiQ920P6.
TreeFamiTF314270.

Enzyme and pathway databases

ReactomeiR-RNO-74217. Purine salvage.

Miscellaneous databases

PROiQ920P6.

Gene expression databases

BgeeiENSRNOG00000010265.
GenevisibleiQ920P6. RN.

Family and domain databases

CDDicd01320. ADA. 1 hit.
HAMAPiMF_00540. A_deaminase. 1 hit.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028893. A_deaminase.
IPR006330. Ado/ade_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01430. aden_deam. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADA_RAT
AccessioniPrimary (citable) accession number: Q920P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.