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Q920P6 (ADA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine deaminase

EC=3.5.4.4
Alternative name(s):
Adenosine aminohydrolase
Gene names
Name:Ada
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion By similarity. HAMAP-Rule MF_00540

Catalytic activity

Adenosine + H2O = inosine + NH3. Ref.4

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00540

Subunit structure

Interacts with DPP4 (extracellular domain) By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein; Extracellular side By similarity. Cell junction By similarity. Cytoplasmic vesicle lumen. Cytoplasm By similarity. Note: Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion By similarity. Ref.3 Ref.4

Tissue specificity

Detected in brain and liver (at protein level). Ref.3 Ref.4

Sequence similarities

Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
   Biological processCell adhesion
Nucleotide metabolism
   Cellular componentCell junction
Cell membrane
Cytoplasm
Cytoplasmic vesicle
Membrane
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processPeyer's patch development

Inferred from electronic annotation. Source: Ensembl

T cell activation

Inferred from Biological aspect of Ancestor. Source: RefGenome

adenosine catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

adenosine metabolic process

Inferred from direct assay PubMed 12675911PubMed 3746429PubMed 4462574PubMed 6815190. Source: RGD

aging

Inferred from expression pattern PubMed 2982653. Source: RGD

cell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

dATP catabolic process

Inferred from electronic annotation. Source: Ensembl

deoxyadenosine catabolic process

Inferred from electronic annotation. Source: Ensembl

embryonic digestive tract development

Inferred from electronic annotation. Source: Ensembl

germinal center B cell differentiation

Inferred from electronic annotation. Source: Ensembl

histamine secretion

Inferred from mutant phenotype PubMed 19066225. Source: RGD

hypoxanthine salvage

Inferred from Biological aspect of Ancestor. Source: RefGenome

inosine biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

liver development

Inferred from electronic annotation. Source: Ensembl

lung alveolus development

Inferred from electronic annotation. Source: Ensembl

negative regulation of adenosine receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

negative regulation of circadian sleep/wake cycle, non-REM sleep

Inferred from mutant phenotype PubMed 19066225. Source: RGD

negative regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

negative regulation of leukocyte migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of mature B cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of mucus secretion

Inferred from electronic annotation. Source: Ensembl

negative regulation of penile erection

Inferred from electronic annotation. Source: Ensembl

negative regulation of thymocyte apoptotic process

Inferred from electronic annotation. Source: Ensembl

placenta development

Inferred from electronic annotation. Source: Ensembl

positive regulation of B cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell differentiation in thymus

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of alpha-beta T cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of calcium-mediated signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of germinal center formation

Inferred from electronic annotation. Source: Ensembl

positive regulation of heart rate

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

purine nucleotide salvage

Inferred from electronic annotation. Source: Ensembl

purine ribonucleoside monophosphate biosynthetic process

Inferred from electronic annotation. Source: InterPro

regulation of cell-cell adhesion mediated by integrin

Inferred from electronic annotation. Source: Ensembl

regulation of circadian sleep/wake cycle, sleep

Inferred from expression pattern PubMed 11038259. Source: RGD

response to drug

Inferred from expression pattern PubMed 16716042. Source: RGD

response to hydrogen peroxide

Inferred from expression pattern PubMed 15966013. Source: RGD

response to hypoxia

Inferred from mutant phenotype PubMed 20735412. Source: RGD

response to morphine

Inferred from mutant phenotype PubMed 19934879. Source: RGD

response to vitamin E

Inferred from direct assay PubMed 16716042. Source: RGD

trophectodermal cell differentiation

Inferred from electronic annotation. Source: Ensembl

xanthine biosynthetic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from direct assay PubMed 4462574. Source: RGD

cytoplasmic membrane-bounded vesicle lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Inferred from Biological aspect of Ancestor. Source: RefGenome

dendrite cytoplasm

Inferred from direct assay PubMed 2253038. Source: RGD

external side of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

extracellular space

Inferred from direct assay PubMed 16563876. Source: RGD

lysosome

Inferred from electronic annotation. Source: Ensembl

neuronal cell body

Inferred from direct assay PubMed 2253038PubMed 3746429. Source: RGD

   Molecular_functionadenosine deaminase activity

Inferred from sequence or structural similarity. Source: UniProtKB

purine nucleoside binding

Inferred from direct assay PubMed 3746429PubMed 4462574PubMed 6815190. Source: RGD

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 352351Adenosine deaminase HAMAP-Rule MF_00540
PRO_0000194354

Sites

Active site2171Proton donor By similarity
Metal binding151Zinc; catalytic By similarity
Metal binding171Zinc; catalytic By similarity
Metal binding2141Zinc; catalytic By similarity
Metal binding2951Zinc; catalytic By similarity
Binding site171Substrate By similarity
Binding site191Substrate By similarity
Binding site1841Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site2961Substrate By similarity
Site2381Important for catalytic activity By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue541N6-acetyllysine By similarity
Modified residue2321N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q920P6 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F689ADFD39682FC6

FASTA35239,899
        10         20         30         40         50         60 
MAQTPAFNKP KVELHVHLDG AIKPETILYY GKKRGIDLPA DTVEGLRNII GMDKPLSLPD 

        70         80         90        100        110        120 
FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVDPIPWNQA 

       130        140        150        160        170        180 
EGDLTPDEVV DLVNQGLQEG EQAFGIKVRS ILCCMRHQPS WSPEVLELCK KYHQKTVVAM 

       190        200        210        220        230        240 
DLAGDETIEG SSLFPGHVEA YEGAVKDGIH RTVHAGEVGS AEVVREAVDI LKTERVGHGY 

       250        260        270        280        290        300 
HTIEDEALYN RLLKENMHFE VCPWSSYLTG AWNPKTTHAV VRFKDDQANY SLNSDDPLIF 

       310        320        330        340        350 
KSTVDTDYQM VKKDMGFTEE EFKRLNINAA KSSFLPEDEK KELLERLYKE YQ 

« Hide

References

« Hide 'large scale' references
[1]"Restricted expression of adenosine deaminase in rat leptomeninges and its role in the regulation of sleep."
Okada T., Mochizuki T., Huag Z., Sugita Y., Urade Y., Hayaishi O.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Leptomeninges.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[3]"Adenosine deaminase in rodent median eminence: detection by antibody to the mouse enzyme and co-localization with adenosine deaminase-complexing protein (CD26)."
Nagy J.I., Yamamoto T., Uemura H., Schrader W.P.
Neuroscience 73:459-471(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]"AMP deaminase and adenosine deaminase activities in liver and brain regions in acute ammonia intoxication and subacute toxic hepatitis."
Kaminsky Y., Kosenko E.
Brain Res. 1311:175-181(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB059655 mRNA. Translation: BAB69691.1.
BC088116 mRNA. Translation: AAH88116.1.
RefSeqNP_569083.1. NM_130399.2.
UniGeneRn.12689.

3D structure databases

ProteinModelPortalQ920P6.
SMRQ920P6. Positions 4-352.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000014151.

PTM databases

PhosphoSiteQ920P6.

Proteomic databases

PaxDbQ920P6.
PRIDEQ920P6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000014151; ENSRNOP00000014151; ENSRNOG00000010265.
GeneID24165.
KEGGrno:24165.
UCSCRGD:2031. rat.

Organism-specific databases

CTD100.
RGD2031. Ada.

Phylogenomic databases

eggNOGCOG1816.
GeneTreeENSGT00730000111151.
HOGENOMHOG000218816.
HOVERGENHBG001718.
InParanoidQ920P6.
KOK01488.
OMALYKAYGM.
OrthoDBEOG7GN2MZ.
PhylomeDBQ920P6.
TreeFamTF314270.

Gene expression databases

GenevestigatorQ920P6.

Family and domain databases

HAMAPMF_00540. A_deaminase.
InterProIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR028893. A_deaminase.
IPR006330. Ado/ade_deaminase.
[Graphical view]
PfamPF00962. A_deaminase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01430. aden_deam. 1 hit.
PROSITEPS00485. A_DEAMINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602469.
PROQ920P6.

Entry information

Entry nameADA_RAT
AccessionPrimary (citable) accession number: Q920P6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families