Q920P6 (ADA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:

Ada

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion By similarity.
Catalytic activity	Adenosine + H2O = inosine + NH3. Ref.4
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with DPP4 (extracellular domain) By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Extracellular side By similarity. Cell junction By similarity. Cytoplasmic vesicle lumen. Cytoplasm By similarity. Note: Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion By similarity. Ref.3 Ref.4
Tissue specificity	Detected in brain and liver (at protein level). Ref.3 Ref.4
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Cell adhesion Nucleotide metabolism
Cellular component	Cell junction Cell membrane Cytoplasm Cytoplasmic vesicle Membrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Peyer's patch development Inferred from electronic annotation. Source: Compara T cell activation Inferred from Biological aspect of Ancestor. Source: RefGenome adenosine catabolic process Inferred from sequence or structural similarity. Source: UniProtKB aging Inferred from expression pattern PubMed 2982653. Source: RGD cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW dATP catabolic process Inferred from electronic annotation. Source: Compara deoxyadenosine catabolic process Inferred from electronic annotation. Source: Compara embryonic digestive tract development Inferred from electronic annotation. Source: Compara germinal center B cell differentiation Inferred from electronic annotation. Source: Compara histamine secretion Inferred from mutant phenotype PubMed 19066225. Source: RGD hypoxanthine salvage Inferred from Biological aspect of Ancestor. Source: RefGenome inosine biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB liver development Inferred from electronic annotation. Source: Compara lung alveolus development Inferred from electronic annotation. Source: Compara negative regulation of adenosine receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of circadian sleep/wake cycle, non-REM sleep Inferred from mutant phenotype PubMed 19066225. Source: RGD negative regulation of inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of leukocyte migration Inferred from electronic annotation. Source: Compara negative regulation of mature B cell apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of mucus secretion Inferred from electronic annotation. Source: Compara negative regulation of penile erection Inferred from electronic annotation. Source: Compara negative regulation of thymocyte apoptotic process Inferred from electronic annotation. Source: Compara placenta development Inferred from electronic annotation. Source: Compara positive regulation of B cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of T cell differentiation in thymus Inferred from electronic annotation. Source: Compara positive regulation of T cell receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of alpha-beta T cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of calcium-mediated signaling Inferred from electronic annotation. Source: Compara positive regulation of germinal center formation Inferred from electronic annotation. Source: Compara positive regulation of heart rate Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara purine nucleotide salvage Inferred from electronic annotation. Source: Compara purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro regulation of cell-cell adhesion mediated by integrin Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from expression pattern PubMed 15966013. Source: RGD response to hypoxia Inferred from mutant phenotype PubMed 20735412. Source: RGD response to morphine Inferred from mutant phenotype PubMed 19934879. Source: RGD response to vitamin E Inferred from direct assay PubMed 16716042. Source: RGD trophectodermal cell differentiation Inferred from electronic annotation. Source: Compara xanthine biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic membrane-bounded vesicle lumen Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome dendrite cytoplasm Inferred from direct assay PubMed 2253038. Source: RGD external side of plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from direct assay PubMed 16563876. Source: RGD lysosome Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from direct assay PubMed 2253038PubMed 3746429. Source: RGD
Molecular_function	adenosine deaminase activity Inferred from sequence or structural similarity. Source: UniProtKB purine nucleoside binding Inferred from direct assay PubMed 3746429PubMed 4462574PubMed 6815190. Source: RGD zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 352	351	Adenosine deaminase		PRO_0000194354
Sites
	Active site	217	1	Proton donor By similarity
	Metal binding	15	1	Zinc; catalytic By similarity
	Metal binding	17	1	Zinc; catalytic By similarity
	Metal binding	214	1	Zinc; catalytic By similarity
	Metal binding	295	1	Zinc; catalytic By similarity
	Binding site	17	1	Substrate By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	184	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	296	1	Substrate By similarity
	Site	238	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	54	1	N6-acetyllysine By similarity
	Modified residue	232	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q920P6 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F689ADFD39682FC6	FASTA	352	39,899
	10 20 30 40 50 60 MAQTPAFNKP KVELHVHLDG AIKPETILYY GKKRGIDLPA DTVEGLRNII GMDKPLSLPD 70 80 90 100 110 120 FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVDPIPWNQA 130 140 150 160 170 180 EGDLTPDEVV DLVNQGLQEG EQAFGIKVRS ILCCMRHQPS WSPEVLELCK KYHQKTVVAM 190 200 210 220 230 240 DLAGDETIEG SSLFPGHVEA YEGAVKDGIH RTVHAGEVGS AEVVREAVDI LKTERVGHGY 250 260 270 280 290 300 HTIEDEALYN RLLKENMHFE VCPWSSYLTG AWNPKTTHAV VRFKDDQANY SLNSDDPLIF 310 320 330 340 350 KSTVDTDYQM VKKDMGFTEE EFKRLNINAA KSSFLPEDEK KELLERLYKE YQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Restricted expression of adenosine deaminase in rat leptomeninges and its role in the regulation of sleep." Okada T., Mochizuki T., Huag Z., Sugita Y., Urade Y., Hayaishi O. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Leptomeninges.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"Adenosine deaminase in rodent median eminence: detection by antibody to the mouse enzyme and co-localization with adenosine deaminase-complexing protein (CD26)." Nagy J.I., Yamamoto T., Uemura H., Schrader W.P. Neuroscience 73:459-471(1996) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]	"AMP deaminase and adenosine deaminase activities in liver and brain regions in acute ammonia intoxication and subacute toxic hepatitis." Kaminsky Y., Kosenko E. Brain Res. 1311:175-181(2010) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB059655 mRNA. Translation: BAB69691.1. BC088116 mRNA. Translation: AAH88116.1.
IPI	IPI00200806.
RefSeq	NP_569083.1. NM_130399.2.
UniGene	Rn.12689.
3D structure databases
ProteinModelPortal	Q920P6.
SMR	Q920P6. Positions 4-352.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000014151.
PTM databases
PhosphoSite	Q920P6.
Proteomic databases
PaxDb	Q920P6.
PRIDE	Q920P6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000014151; ENSRNOP00000014151; ENSRNOG00000010265.
GeneID	24165.
KEGG	rno:24165.
UCSC	RGD:2031. rat.
Organism-specific databases
CTD	100.
RGD	2031. Ada.
Phylogenomic databases
eggNOG	COG1816.
GeneTree	ENSGT00640000091458.
HOGENOM	HOG000218816.
HOVERGEN	HBG001718.
InParanoid	Q920P6.
KO	K01488.
OMA	MPAIAGC.
OrthoDB	EOG40K808.
Gene expression databases
Genevestigator	Q920P6.
GermOnline	ENSRNOG00000010265. Rattus norvegicus.
Family and domain databases
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase_dom. IPR006330. Ado/ade_deaminase. [Graphical view]
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	602469.

Entry information

Entry name

ADA_RAT

Accession

Primary (citable) accession number: Q920P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program