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Protein

Adenylate kinase isoenzyme 5

Gene

Ak5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.By similarity

Catalytic activityi

ATP + AMP = 2 ADP.By similarity
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168AMP 1By similarity1
Binding sitei226AMP 1By similarity1
Binding sitei257ATP 1By similarity1
Binding sitei263AMP 1By similarity1
Binding sitei274AMP 1By similarity1
Binding sitei407AMP 2By similarity1
Binding sitei412AMP 2By similarity1
Binding sitei469AMP 2By similarity1
Binding sitei500ATP 2By similarity1
Binding sitei506AMP 2By similarity1
Binding sitei517AMP 2By similarity1
Binding sitei545ATP 2; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi142 – 147ATP 1By similarity6
Nucleotide bindingi191 – 193AMP 1By similarity3
Nucleotide bindingi219 – 222AMP 1By similarity4
Nucleotide bindingi386 – 391ATP 2By similarity6
Nucleotide bindingi433 – 435AMP 2By similarity3
Nucleotide bindingi462 – 465AMP 2By similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 5 (EC:2.7.4.3, EC:2.7.4.6)
Short name:
AK 5
Alternative name(s):
ATP-AMP transphosphorylase 5
Gene namesi
Name:Ak5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2677491. Ak5.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589311 – 562Adenylate kinase isoenzyme 5Add BLAST562

Proteomic databases

PaxDbiQ920P5.
PeptideAtlasiQ920P5.
PRIDEiQ920P5.

PTM databases

iPTMnetiQ920P5.
PhosphoSitePlusiQ920P5.

Expressioni

Tissue specificityi

Brain specific.By similarity

Gene expression databases

BgeeiENSMUSG00000039058.
CleanExiMM_AK5.
ExpressionAtlasiQ920P5. baseline and differential.
GenevisibleiQ920P5. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi230921. 1 interactor.
IntActiQ920P5. 1 interactor.
STRINGi10090.ENSMUSP00000042785.

Structurei

3D structure databases

ProteinModelPortaliQ920P5.
SMRiQ920P5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 316Adenylate kinase 1By similarityAdd BLAST184
Regioni162 – 193NMPbind 1By similarityAdd BLAST32
Regioni256 – 266LID 1By similarityAdd BLAST11
Regioni377 – 559Adenylate kinase 2By similarityAdd BLAST183
Regioni406 – 435NMPbind 2By similarityAdd BLAST30
Regioni499 – 509LID 2By similarityAdd BLAST11

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOGENOMiHOG000286022.
HOVERGENiHBG059001.
InParanoidiQ920P5.
KOiK00939.
OMAiHINMTDL.
OrthoDBiEOG091G0OQ7.
PhylomeDBiQ920P5.
TreeFamiTF313747.

Family and domain databases

CDDicd01428. ADK. 2 hits.
Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 2 hits.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR006267. AK1/5.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q920P5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTNDAKEYL ARRDIPQLFE SLLNGLMCSK PEDPIEYLET CLQKVKELGG
60 70 80 90 100
CDKVKWDTFV SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP
110 120 130 140 150
IHQFSIESDT DLSETAELIE EYEVFDPTRP RPKIILVIGG PGSGKGTQSL
160 170 180 190 200
KIAERYGFQY ISVGELLRKK IHSASSNRKW SLIAKIITNG ELAPQETTIT
210 220 230 240 250
EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV VFLACANQRL
260 270 280 290 300
KERLQKRAEQ QGRPDDNLKA TQRRLVNFKQ NAAPLVKYFQ EKGLIVTFDA
310 320 330 340 350
DRDEDAVFHD ISVAVDSKLF PNKEAPMDSS DLDPSMMFDA GEIIDTGSDY
360 370 380 390 400
DNQDDDQLNV FGEDTEGGFM EDLRKCKIIF LMGGPGSGKG TQCEKLAEKY
410 420 430 440 450
GFTHLSTGEL LRQELTSESE RSKLIRDIME RGDLVPSGVV LELLKEAMVA
460 470 480 490 500
SLGNTKGFLI DGYPREVKQG EEFGRRIGDP HLVICMDCSA DTMTNRLLQR
510 520 530 540 550
SQSSQRGEDG AKSIAKRLEA YHRASIPVVT YYERKTQLRK VNAEGTPEQV
560
FLQLCTAIDS VF
Length:562
Mass (Da):63,323
Last modified:September 1, 2009 - v2
Checksum:iD94CEC3D3875D055
GO
Isoform 2 (identifier: Q920P5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-369: Missing.

Show »
Length:193
Mass (Da):21,546
Checksum:iF9CB4A595B1825C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti249R → K in AK053807 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378801 – 369Missing in isoform 2. 1 PublicationAdd BLAST369

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB060081 mRNA. Translation: BAB69859.1.
AK053807 mRNA. No translation available.
AC111139 Genomic DNA. No translation available.
AC116720 Genomic DNA. No translation available.
CCDSiCCDS38675.1. [Q920P5-1]
RefSeqiNP_001074746.1. NM_001081277.1. [Q920P5-1]
UniGeneiMm.207057.

Genome annotation databases

EnsembliENSMUST00000045262; ENSMUSP00000042785; ENSMUSG00000039058. [Q920P5-1]
GeneIDi229949.
KEGGimmu:229949.
UCSCiuc008rtq.1. mouse. [Q920P5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB060081 mRNA. Translation: BAB69859.1.
AK053807 mRNA. No translation available.
AC111139 Genomic DNA. No translation available.
AC116720 Genomic DNA. No translation available.
CCDSiCCDS38675.1. [Q920P5-1]
RefSeqiNP_001074746.1. NM_001081277.1. [Q920P5-1]
UniGeneiMm.207057.

3D structure databases

ProteinModelPortaliQ920P5.
SMRiQ920P5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230921. 1 interactor.
IntActiQ920P5. 1 interactor.
STRINGi10090.ENSMUSP00000042785.

PTM databases

iPTMnetiQ920P5.
PhosphoSitePlusiQ920P5.

Proteomic databases

PaxDbiQ920P5.
PeptideAtlasiQ920P5.
PRIDEiQ920P5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045262; ENSMUSP00000042785; ENSMUSG00000039058. [Q920P5-1]
GeneIDi229949.
KEGGimmu:229949.
UCSCiuc008rtq.1. mouse. [Q920P5-1]

Organism-specific databases

CTDi26289.
MGIiMGI:2677491. Ak5.

Phylogenomic databases

eggNOGiKOG3079. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00390000016215.
HOGENOMiHOG000286022.
HOVERGENiHBG059001.
InParanoidiQ920P5.
KOiK00939.
OMAiHINMTDL.
OrthoDBiEOG091G0OQ7.
PhylomeDBiQ920P5.
TreeFamiTF313747.

Enzyme and pathway databases

ReactomeiR-MMU-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

ChiTaRSiAk5. mouse.
PROiQ920P5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039058.
CleanExiMM_AK5.
ExpressionAtlasiQ920P5. baseline and differential.
GenevisibleiQ920P5. MM.

Family and domain databases

CDDicd01428. ADK. 2 hits.
Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00235. Adenylate_kinase_Adk. 2 hits.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR006267. AK1/5.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01360. aden_kin_iso1. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD5_MOUSE
AccessioniPrimary (citable) accession number: Q920P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.