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Protein

Adenylate kinase isoenzyme 5

Gene

Ak5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.By similarity

Catalytic activityi

ATP + AMP = 2 ADP.By similarity
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168AMP 1By similarity1
Binding sitei226AMP 1By similarity1
Binding sitei257ATP 1By similarity1
Binding sitei263AMP 1By similarity1
Binding sitei274AMP 1By similarity1
Binding sitei407AMP 2By similarity1
Binding sitei412AMP 2By similarity1
Binding sitei469AMP 2By similarity1
Binding sitei500ATP 2By similarity1
Binding sitei506AMP 2By similarity1
Binding sitei517AMP 2By similarity1
Binding sitei545ATP 2; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi142 – 147ATP 1By similarity6
Nucleotide bindingi191 – 193AMP 1By similarity3
Nucleotide bindingi219 – 222AMP 1By similarity4
Nucleotide bindingi386 – 391ATP 2By similarity6
Nucleotide bindingi433 – 435AMP 2By similarity3
Nucleotide bindingi462 – 465AMP 2By similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 5 (EC:2.7.4.3, EC:2.7.4.6)
Short name:
AK 5
Alternative name(s):
ATP-AMP transphosphorylase 5
Gene namesi
Name:Ak5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2677491 Ak5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589311 – 562Adenylate kinase isoenzyme 5Add BLAST562

Proteomic databases

PaxDbiQ920P5
PeptideAtlasiQ920P5
PRIDEiQ920P5

PTM databases

iPTMnetiQ920P5
PhosphoSitePlusiQ920P5

Expressioni

Tissue specificityi

Brain specific.By similarity

Gene expression databases

BgeeiENSMUSG00000039058
CleanExiMM_AK5
ExpressionAtlasiQ920P5 baseline and differential
GenevisibleiQ920P5 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi230921, 2 interactors
IntActiQ920P5, 1 interactor
STRINGi10090.ENSMUSP00000042785

Structurei

3D structure databases

ProteinModelPortaliQ920P5
SMRiQ920P5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 316Adenylate kinase 1By similarityAdd BLAST184
Regioni162 – 193NMPbind 1By similarityAdd BLAST32
Regioni256 – 266LID 1By similarityAdd BLAST11
Regioni377 – 559Adenylate kinase 2By similarityAdd BLAST183
Regioni406 – 435NMPbind 2By similarityAdd BLAST30
Regioni499 – 509LID 2By similarityAdd BLAST11

Sequence similaritiesi

Belongs to the adenylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3079 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00390000016215
HOGENOMiHOG000286022
HOVERGENiHBG059001
InParanoidiQ920P5
KOiK00939
OMAiFGEDTMG
OrthoDBiEOG091G0OQ7
PhylomeDBiQ920P5
TreeFamiTF313747

Family and domain databases

CDDicd01428 ADK, 2 hits
HAMAPiMF_00235 Adenylate_kinase_Adk, 2 hits
InterProiView protein in InterPro
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR006267 AK1/5
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR01360 aden_kin_iso1, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q920P5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTNDAKEYL ARRDIPQLFE SLLNGLMCSK PEDPIEYLET CLQKVKELGG
60 70 80 90 100
CDKVKWDTFV SQEKKTLPPL NGGQSRRSFL RNVMPENSNF PYRRYDRLPP
110 120 130 140 150
IHQFSIESDT DLSETAELIE EYEVFDPTRP RPKIILVIGG PGSGKGTQSL
160 170 180 190 200
KIAERYGFQY ISVGELLRKK IHSASSNRKW SLIAKIITNG ELAPQETTIT
210 220 230 240 250
EIKQKLMQIP DEEGIVIDGF PRDVAQALSF EDQICTPDLV VFLACANQRL
260 270 280 290 300
KERLQKRAEQ QGRPDDNLKA TQRRLVNFKQ NAAPLVKYFQ EKGLIVTFDA
310 320 330 340 350
DRDEDAVFHD ISVAVDSKLF PNKEAPMDSS DLDPSMMFDA GEIIDTGSDY
360 370 380 390 400
DNQDDDQLNV FGEDTEGGFM EDLRKCKIIF LMGGPGSGKG TQCEKLAEKY
410 420 430 440 450
GFTHLSTGEL LRQELTSESE RSKLIRDIME RGDLVPSGVV LELLKEAMVA
460 470 480 490 500
SLGNTKGFLI DGYPREVKQG EEFGRRIGDP HLVICMDCSA DTMTNRLLQR
510 520 530 540 550
SQSSQRGEDG AKSIAKRLEA YHRASIPVVT YYERKTQLRK VNAEGTPEQV
560
FLQLCTAIDS VF
Length:562
Mass (Da):63,323
Last modified:September 1, 2009 - v2
Checksum:iD94CEC3D3875D055
GO
Isoform 2 (identifier: Q920P5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-369: Missing.

Show »
Length:193
Mass (Da):21,546
Checksum:iF9CB4A595B1825C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti249R → K in AK053807 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378801 – 369Missing in isoform 2. 1 PublicationAdd BLAST369

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB060081 mRNA Translation: BAB69859.1
AK053807 mRNA No translation available.
AC111139 Genomic DNA No translation available.
AC116720 Genomic DNA No translation available.
CCDSiCCDS38675.1 [Q920P5-1]
RefSeqiNP_001074746.1, NM_001081277.1 [Q920P5-1]
UniGeneiMm.207057

Genome annotation databases

EnsembliENSMUST00000045262; ENSMUSP00000042785; ENSMUSG00000039058 [Q920P5-1]
GeneIDi229949
KEGGimmu:229949
UCSCiuc008rtq.1 mouse [Q920P5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKAD5_MOUSE
AccessioniPrimary (citable) accession number: Q920P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: September 1, 2009
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health