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Protein

L-xylulose reductase

Gene

Dcxr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.

Catalytic activityi

Xylitol + NADP+ = L-xylulose + NADPH.3 Publications

Kineticsi

  1. KM=0.11 mM for L-xylulose (at 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei136SubstrateBy similarity1
    Active sitei149Proton acceptor1
    Active sitei1531

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi11 – 39NADPBy similarityAdd BLAST29

    GO - Molecular functioni

    GO - Biological processi

    • D-xylose metabolic process Source: UniProtKB-KW
    • glucose metabolic process Source: UniProtKB
    • NADP metabolic process Source: Ensembl
    • protein homotetramerization Source: UniProtKB
    • xylulose metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Xylose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BRENDAi1.1.1.10. 5301.
    ReactomeiR-RNO-5661270. Catabolism of glucuronate to xylulose-5-phosphate.
    SABIO-RKQ920P0.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-xylulose reductase (EC:1.1.1.103 Publications)
    Short name:
    XR
    Alternative name(s):
    Dicarbonyl/L-xylulose reductase
    Gene namesi
    Name:Dcxr
    Synonyms:Glb
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 10

    Organism-specific databases

    RGDi620031. Dcxr.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi136S → A: Loss of activity. 1 Publication1
    Mutagenesisi137Q → M: Slightly reduced activity. Loss of activity for sugars; when associated with F-143 and L-146. 1 Publication1
    Mutagenesisi143L → F: Slightly reduced activity. Loss of activity for sugars; when associated with M-137 and L-146. 1 Publication1
    Mutagenesisi146H → L: Slightly reduced activity. Loss of activity for sugars; when associated with M-137; F-143 and L-146. 1 Publication1
    Mutagenesisi149Y → F: Loss of activity. 1 Publication1
    Mutagenesisi153K → M: Loss of activity. 1 Publication1
    Mutagenesisi190N → V: Slightly reduced activity. Loss of activity for sugars; when associated with S-191. 1 Publication1
    Mutagenesisi191W → F: Slightly reduced activity. 1 Publication1
    Mutagenesisi191W → S: Slightly reduced activity. Loss of activity for sugars; when associated with V-190. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000545571 – 244L-xylulose reductaseAdd BLAST244

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1N-acetylmethionineBy similarity1
    Modified residuei21Omega-N-methylarginineBy similarity1
    Modified residuei46PhosphoserineBy similarity1

    Keywords - PTMi

    Acetylation, Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ920P0.
    PRIDEiQ920P0.

    PTM databases

    iPTMnetiQ920P0.
    PhosphoSitePlusiQ920P0.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney and liver. Weakly or not expressed in brain, heart, lung, spleen, epididymis and testis.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000050315.
    ExpressionAtlasiQ920P0. baseline and differential.
    GenevisibleiQ920P0. RN.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    MINTiMINT-4576313.
    STRINGi10116.ENSRNOP00000064161.

    Structurei

    3D structure databases

    ProteinModelPortaliQ920P0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG1207. Eukaryota.
    ENOG410XQCY. LUCA.
    GeneTreeiENSGT00760000118868.
    HOVERGENiHBG105069.
    InParanoidiQ920P0.
    KOiK03331.
    OMAiWSDPCKA.
    OrthoDBiEOG091G0GV2.
    PhylomeDBiQ920P0.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q920P0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDLGLAGRRA LVTGAGKGIG RSTVLALQAA GAQVVAVSRT REDLDSLVRE
    60 70 80 90 100
    CPGVEPVCVD LADWEATEQA LSNVGPVDLL VNNAAVATLQ PFLEVTKEAC
    110 120 130 140 150
    DTSFNVNFRA VVQVSQIVAR GMIARGVPGA IVNVSSQASQ RALTNHTVYC
    160 170 180 190 200
    STKGALDMLT KVMALELGPH KIRVNAVNPT VVMTPMGRAN WSDPHKAKVM
    210 220 230 240
    LDRIPLGKFA EVENVVDTIL FLLSNRSSMT TGSALPVDGG FLAT
    Length:244
    Mass (Da):25,720
    Last modified:December 1, 2001 - v1
    Checksum:i7BAEE7E7BB404057
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB061719 mRNA. Translation: BAB64340.1.
    RefSeqiNP_599214.1. NM_134387.1.
    XP_008766687.1. XM_008768465.2.
    UniGeneiRn.177518.

    Genome annotation databases

    EnsembliENSRNOT00000074362; ENSRNOP00000064161; ENSRNOG00000050315.
    GeneIDi171408.
    KEGGirno:171408.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB061719 mRNA. Translation: BAB64340.1.
    RefSeqiNP_599214.1. NM_134387.1.
    XP_008766687.1. XM_008768465.2.
    UniGeneiRn.177518.

    3D structure databases

    ProteinModelPortaliQ920P0.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    MINTiMINT-4576313.
    STRINGi10116.ENSRNOP00000064161.

    PTM databases

    iPTMnetiQ920P0.
    PhosphoSitePlusiQ920P0.

    Proteomic databases

    PaxDbiQ920P0.
    PRIDEiQ920P0.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000074362; ENSRNOP00000064161; ENSRNOG00000050315.
    GeneIDi171408.
    KEGGirno:171408.

    Organism-specific databases

    CTDi51181.
    RGDi620031. Dcxr.

    Phylogenomic databases

    eggNOGiKOG1207. Eukaryota.
    ENOG410XQCY. LUCA.
    GeneTreeiENSGT00760000118868.
    HOVERGENiHBG105069.
    InParanoidiQ920P0.
    KOiK03331.
    OMAiWSDPCKA.
    OrthoDBiEOG091G0GV2.
    PhylomeDBiQ920P0.

    Enzyme and pathway databases

    BRENDAi1.1.1.10. 5301.
    ReactomeiR-RNO-5661270. Catabolism of glucuronate to xylulose-5-phosphate.
    SABIO-RKQ920P0.

    Miscellaneous databases

    PROiQ920P0.

    Gene expression databases

    BgeeiENSRNOG00000050315.
    ExpressionAtlasiQ920P0. baseline and differential.
    GenevisibleiQ920P0. RN.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    IPR002347. SDR_fam.
    [Graphical view]
    PANTHERiPTHR24322. PTHR24322. 2 hits.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDCXR_RAT
    AccessioniPrimary (citable) accession number: Q920P0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: December 1, 2001
    Last modified: November 2, 2016
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.