Reviewed, UniProtKB/Swiss-Prot Q920N9 (DCXR_CAVPO)

Names and origin

Protein names

Recommended name:
    L-xylulose reductase
      Short name=XR
    EC=1.1.1.10
Alternative name(s):
    Dicarbonyl/L-xylulose reductase
    Protein P26h

Gene names

Name:	DCXR
Synonyms:	GLB

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
Catalytic activity	Xylitol + NADP+ = L-xylulose + NADPH. Ref.1
Subunit structure	Homotetramer.
Subcellular location	Membrane; Peripheral membrane protein By similarity. Note: Probably recruited to membranes via an interaction with phosphatidylinositol By similarity.
Tissue specificity	Highly expressed in kidney and liver. Expressed in epididymis. Expressed at intermediate level in lung. Weakly expressed in brain, heart, spleen and testis. Ref.1
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism Xylose metabolism
Cellular component	Membrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-xylulose reductase (NADP+) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	L-xylulose reductase		PRO_0000054553
Regions
	Nucleotide binding	11 – 39	29	NADP By similarity
Sites
	Active site	149	1	Proton acceptor By similarity
	Active site	153	1	By similarity
	Binding site	136	1	Substrate By similarity
Amino acid modifications
	Modified residue	149	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q920N9-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: C7AE9AE263C59B5A	FASTA	244	25,750
	10 20 30 40 50 60 MDLGLAGRRA LVTGAGKGIG RSTVLALKAA GAQVVAVSRT REDLDDLVRE CPGVEPVCVD 70 80 90 100 110 120 LADWEATEQA LSNVGPADLL VNNAAVALLQ PFLEVTKEAC VTSFNVNLRA VIQVSQIVAK 130 140 150 160 170 180 GMIARGVPGA IVNVSSQASQ RALTNHTVYC STKGALYMLT KMMALELGPH KIRVNAVNPT 190 200 210 220 230 240 VVMTPMGRTN WSDPHKAKAM LDRIPLGKFA EVENVVDTIL FLLSNRSGMT TGSTLPVDGG FLAT

References

[1]

"Molecular characterization of mammalian dicarbonyl/L-xylulose reductase and its localization in kidney." Nakagawa J., Ishikura S., Asami J., Isaji T., Usami N., Hara A., Sakurai T., Tsuritani K., Oda K., Takahashi M., Yoshimoto M., Otsuka N., Kitamura K. J. Biol. Chem. 277:17883-17891(2002) [PubMed: 11882650] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY. Strain: Hartley. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB061720 mRNA. Translation: BAB64341.1.
3D structure databases
SMR	Q920N9. Positions 1-243.
ModBase	Search...
Phylogenomic databases
eggNOG	roNOG13281.
HOVERGEN	Q920N9.
Enzyme and pathway databases
BRENDA	1.1.1.10. 44.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCXR_CAVPO

Accession

Primary (citable) accession number: Q920N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	December 1, 2001
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)