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Protein

Biotin--protein ligase

Gene

Hlcs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase (By similarity).By similarity

Catalytic activityi

ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxytransferase] = AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxytransferase].
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-196780. Biotin transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin--protein ligase (EC:6.3.4.-)
Alternative name(s):
Biotin apo-protein ligase
Including the following 4 domains:
Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:6.3.4.9)
Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC:6.3.4.10)
Alternative name(s):
Holocarboxylase synthetase
Short name:
HCS
Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11)
Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15)
Gene namesi
Name:Hlcs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:894646. Hlcs.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649801 – 722Biotin--protein ligaseAdd BLAST722

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ920N2.
PaxDbiQ920N2.
PRIDEiQ920N2.

PTM databases

iPTMnetiQ920N2.
PhosphoSitePlusiQ920N2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040820.
ExpressionAtlasiQ920N2. baseline and differential.
GenevisibleiQ920N2. MM.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ920N2. 1 interactor.
MINTiMINT-4089341.
STRINGi10090.ENSMUSP00000097112.

Structurei

3D structure databases

ProteinModelPortaliQ920N2.
SMRiQ920N2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini459 – 648BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST190

Sequence similaritiesi

Belongs to the biotin--protein ligase family.Curated

Phylogenomic databases

eggNOGiKOG1536. Eukaryota.
COG0340. LUCA.
GeneTreeiENSGT00390000002960.
HOGENOMiHOG000095254.
HOVERGENiHBG004872.
InParanoidiQ920N2.
KOiK01942.
OMAiRDPLMQW.
OrthoDBiEOG091G0G5R.
PhylomeDBiQ920N2.
TreeFamiTF105860.

Family and domain databases

CDDicd16442. BPL. 1 hit.
InterProiView protein in InterPro
IPR019197. Biotin-prot_ligase_N.
IPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LPL_catalytic.
PANTHERiPTHR12835:SF10. PTHR12835:SF10. 1 hit.
PfamiView protein in Pfam
PF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
PF09825. BPL_N. 1 hit.
TIGRFAMsiTIGR00121. birA_ligase. 1 hit.
PROSITEiView protein in PROSITE
PS51733. BPL_LPL_CATALYTIC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q920N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDRLQMDNG LIAQKIVSVH LKDPALKELG KASDKQVQGP PPGPEASPEA
60 70 80 90 100
QPAQGVMEHA GQGDCKAAGE GPSPRRRGCA PESEPAADGD PGLSSPELCQ
110 120 130 140 150
LHLSICHECL ELENSTIDSV RSASAENIPD LPCDHSGVEG AAGELCPERK
160 170 180 190 200
GKRVNISGKA PNILLYVGSG SEEALGRLQQ VRSVLTDCVD TDSYTLYHLL
210 220 230 240 250
EDSALRDPWS DNCLLLVIAS RDPIPKDIQH KFMAYLSQGG KVLGLSSPFT
260 270 280 290 300
LGGFRVTRRD VLRNTVQNLV FSKADGTEVR LSVLSSGYVY EEGPSLGRLQ
310 320 330 340 350
GHLENEDKDK MIVHVPFGTL GGEAVLCQVH LELPPGASLV QTADDFNVLK
360 370 380 390 400
SSNVRRHEVL KEILTALGLS CDAPQVPALT PLYLLLAAEE TQDPFMQWLG
410 420 430 440 450
RHTDPEGIIK SSKLSLQFVS SYTSEAEITP SSMPVVTDPE AFSSEHFSLE
460 470 480 490 500
TYRQNLQTTR LGKVILFAEV TSTTMSLLDG LMFEMPQEMG LIAIAVRQTQ
510 520 530 540 550
GKGRGPNAWL SPVGCALSTL LVFIPLRSQL GQRIPFVQHL MSLAVVEAVR
560 570 580 590 600
SIPGYEDINL RVKWPNDIYY SDLMKIGGVL VNSTLMGETF YILIGCGFNV
610 620 630 640 650
TNSNPTICIN DLIEEHNKQH GAGLKPLRAD CLIARAVTVL EKLIDRFQDQ
660 670 680 690 700
GPDGVLPLYY KYWVHGGQQV RLGSTEGPQA SIVGLDDSGF LQVHQEDGGV
710 720
VTVHPDGNSF DMLRNLIVPK RQ
Length:722
Mass (Da):78,516
Last modified:December 1, 2001 - v1
Checksum:i93FD61FF31AA7A65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB066227 mRNA. Translation: BAB68213.1.
BC050090 mRNA. Translation: AAH50090.1.
CCDSiCCDS28346.1.
RefSeqiNP_631884.1. NM_139145.4.
XP_006522918.1. XM_006522855.2.
XP_006522919.1. XM_006522856.2.
UniGeneiMm.30921.

Genome annotation databases

EnsembliENSMUST00000099512; ENSMUSP00000097112; ENSMUSG00000040820.
ENSMUST00000163193; ENSMUSP00000130981; ENSMUSG00000040820.
GeneIDi110948.
KEGGimmu:110948.
UCSCiuc008aag.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiBPL1_MOUSE
AccessioniPrimary (citable) accession number: Q920N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: December 1, 2001
Last modified: September 27, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families