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Protein

Biotin--protein ligase

Gene

Hlcs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Post-translational modification of specific protein by attachment of biotin. Acts on various carboxylases such as acetyl-CoA-carboxylase, pyruvate carboxylase, propionyl CoA carboxylase, and 3-methylcrotonyl CoA carboxylase (By similarity).By similarity

Catalytic activityi

ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxytransferase] = AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxytransferase].
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [propionyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3-methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)].
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-196780. Biotin transport and metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin--protein ligase (EC:6.3.4.-)
Alternative name(s):
Biotin apo-protein ligase
Including the following 4 domains:
Biotin--[methylmalonyl-CoA-carboxytransferase] ligase (EC:6.3.4.9)
Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase (EC:6.3.4.10)
Alternative name(s):
Holocarboxylase synthetase
Short name:
HCS
Biotin--[methylcrotonoyl-CoA-carboxylase] ligase (EC:6.3.4.11)
Biotin--[acetyl-CoA-carboxylase] ligase (EC:6.3.4.15)
Gene namesi
Name:Hlcs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:894646. Hlcs.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: BHF-UCL
  • cytoplasm Source: BHF-UCL
  • cytosol Source: BHF-UCL
  • mitochondrion Source: MGI
  • nuclear lamina Source: BHF-UCL
  • nuclear matrix Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Biotin--protein ligasePRO_0000064980Add
BLAST

Proteomic databases

EPDiQ920N2.
MaxQBiQ920N2.
PaxDbiQ920N2.
PRIDEiQ920N2.

PTM databases

iPTMnetiQ920N2.
PhosphoSiteiQ920N2.

Expressioni

Gene expression databases

BgeeiQ920N2.
ExpressionAtlasiQ920N2. baseline and differential.
GenevisibleiQ920N2. MM.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ920N2. 1 interaction.
MINTiMINT-4089341.
STRINGi10090.ENSMUSP00000097112.

Structurei

3D structure databases

ProteinModelPortaliQ920N2.
SMRiQ920N2. Positions 456-703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini459 – 648190BPL/LPL catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the biotin--protein ligase family.Curated
Contains 1 BPL/LPL catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1536. Eukaryota.
COG0340. LUCA.
GeneTreeiENSGT00390000002960.
HOGENOMiHOG000095254.
HOVERGENiHBG004872.
InParanoidiQ920N2.
KOiK01942.
OMAiRDPLMQW.
OrthoDBiEOG7SV0TT.
PhylomeDBiQ920N2.
TreeFamiTF105860.

Family and domain databases

InterProiIPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LPL_catalytic.
[Graphical view]
PANTHERiPTHR12835. PTHR12835. 1 hit.
PfamiPF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00121. birA_ligase. 1 hit.
PROSITEiPS51733. BPL_LPL_CATALYTIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q920N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDRLQMDNG LIAQKIVSVH LKDPALKELG KASDKQVQGP PPGPEASPEA
60 70 80 90 100
QPAQGVMEHA GQGDCKAAGE GPSPRRRGCA PESEPAADGD PGLSSPELCQ
110 120 130 140 150
LHLSICHECL ELENSTIDSV RSASAENIPD LPCDHSGVEG AAGELCPERK
160 170 180 190 200
GKRVNISGKA PNILLYVGSG SEEALGRLQQ VRSVLTDCVD TDSYTLYHLL
210 220 230 240 250
EDSALRDPWS DNCLLLVIAS RDPIPKDIQH KFMAYLSQGG KVLGLSSPFT
260 270 280 290 300
LGGFRVTRRD VLRNTVQNLV FSKADGTEVR LSVLSSGYVY EEGPSLGRLQ
310 320 330 340 350
GHLENEDKDK MIVHVPFGTL GGEAVLCQVH LELPPGASLV QTADDFNVLK
360 370 380 390 400
SSNVRRHEVL KEILTALGLS CDAPQVPALT PLYLLLAAEE TQDPFMQWLG
410 420 430 440 450
RHTDPEGIIK SSKLSLQFVS SYTSEAEITP SSMPVVTDPE AFSSEHFSLE
460 470 480 490 500
TYRQNLQTTR LGKVILFAEV TSTTMSLLDG LMFEMPQEMG LIAIAVRQTQ
510 520 530 540 550
GKGRGPNAWL SPVGCALSTL LVFIPLRSQL GQRIPFVQHL MSLAVVEAVR
560 570 580 590 600
SIPGYEDINL RVKWPNDIYY SDLMKIGGVL VNSTLMGETF YILIGCGFNV
610 620 630 640 650
TNSNPTICIN DLIEEHNKQH GAGLKPLRAD CLIARAVTVL EKLIDRFQDQ
660 670 680 690 700
GPDGVLPLYY KYWVHGGQQV RLGSTEGPQA SIVGLDDSGF LQVHQEDGGV
710 720
VTVHPDGNSF DMLRNLIVPK RQ
Length:722
Mass (Da):78,516
Last modified:December 1, 2001 - v1
Checksum:i93FD61FF31AA7A65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB066227 mRNA. Translation: BAB68213.1.
BC050090 mRNA. Translation: AAH50090.1.
CCDSiCCDS28346.1.
RefSeqiNP_631884.1. NM_139145.4.
XP_006522918.1. XM_006522855.1.
XP_006522919.1. XM_006522856.1.
XP_011249711.1. XM_011251409.1.
UniGeneiMm.30921.

Genome annotation databases

EnsembliENSMUST00000099512; ENSMUSP00000097112; ENSMUSG00000040820.
ENSMUST00000163193; ENSMUSP00000130981; ENSMUSG00000040820.
GeneIDi110948.
KEGGimmu:110948.
UCSCiuc008aag.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB066227 mRNA. Translation: BAB68213.1.
BC050090 mRNA. Translation: AAH50090.1.
CCDSiCCDS28346.1.
RefSeqiNP_631884.1. NM_139145.4.
XP_006522918.1. XM_006522855.1.
XP_006522919.1. XM_006522856.1.
XP_011249711.1. XM_011251409.1.
UniGeneiMm.30921.

3D structure databases

ProteinModelPortaliQ920N2.
SMRiQ920N2. Positions 456-703.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ920N2. 1 interaction.
MINTiMINT-4089341.
STRINGi10090.ENSMUSP00000097112.

PTM databases

iPTMnetiQ920N2.
PhosphoSiteiQ920N2.

Proteomic databases

EPDiQ920N2.
MaxQBiQ920N2.
PaxDbiQ920N2.
PRIDEiQ920N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099512; ENSMUSP00000097112; ENSMUSG00000040820.
ENSMUST00000163193; ENSMUSP00000130981; ENSMUSG00000040820.
GeneIDi110948.
KEGGimmu:110948.
UCSCiuc008aag.1. mouse.

Organism-specific databases

CTDi3141.
MGIiMGI:894646. Hlcs.

Phylogenomic databases

eggNOGiKOG1536. Eukaryota.
COG0340. LUCA.
GeneTreeiENSGT00390000002960.
HOGENOMiHOG000095254.
HOVERGENiHBG004872.
InParanoidiQ920N2.
KOiK01942.
OMAiRDPLMQW.
OrthoDBiEOG7SV0TT.
PhylomeDBiQ920N2.
TreeFamiTF105860.

Enzyme and pathway databases

ReactomeiR-MMU-196780. Biotin transport and metabolism.

Miscellaneous databases

ChiTaRSiHlcs. mouse.
NextBioi364997.
PROiQ920N2.
SOURCEiSearch...

Gene expression databases

BgeeiQ920N2.
ExpressionAtlasiQ920N2. baseline and differential.
GenevisibleiQ920N2. MM.

Family and domain databases

InterProiIPR004408. Biotin_CoA_COase_ligase.
IPR003142. BPL_C.
IPR004143. BPL_LPL_catalytic.
[Graphical view]
PANTHERiPTHR12835. PTHR12835. 1 hit.
PfamiPF02237. BPL_C. 1 hit.
PF03099. BPL_LplA_LipB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00121. birA_ligase. 1 hit.
PROSITEiPS51733. BPL_LPL_CATALYTIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Hattori M., Ishii K., Toyoda A., Taylor T.D., Hong-Seog P., Fujiyama A., Yada T., Totoki Y., Watanabe H., Sakaki Y.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Testis.

Entry informationi

Entry nameiBPL1_MOUSE
AccessioniPrimary (citable) accession number: Q920N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: December 1, 2001
Last modified: March 16, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.