E3 ubiquitin-protein ligase SIAH1 - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot Q920M9 (SIAH1_RAT)

Last modified November 3, 2009. Version 64. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    E3 ubiquitin-protein ligase SIAH1
    EC=6.3.2.-
Alternative name(s):
    Seven in absentia homolog 1
      Short name=Siah-1
    Siah-1a

Gene names

Name:	Siah1
Synonyms:	Siah1a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), cytoplasmic signal transduction molecules (TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3 By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homodimer. Component of some large E3 complex composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X. Interacts with UBE2I. Interacts with alpha-tubulin. Interacts with PEG10, which may inhibit its activity. Interacts with PEG3 By similarity. Interacts with group 1 glutamate receptors GRM1 and GRM5. Interacts with DAB1, which may inhibit its activity. Interacts with UBE2E2.
Subcellular location	Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Partially nuclear. Ref.1
Domain	The RING-type zinc finger domain is essential for ubiquitin ligase activity By similarity. The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family By similarity.
Sequence similarities	Belongs to the SINA (Seven in absentia) family. Contains 1 RING-type zinc finger. Contains 1 SIAH-type zinc finger.

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Ontologies

Keywords
Biological process	Apoptosis Cell cycle Differentiation Spermatogenesis Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Developmental protein Ligase
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination during ubiquitin-dependent protein catabolic process Ref.1 Inferred from direct assay. Source: RGD spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	early endosome Ref.1 Inferred from direct assay. Source: RGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle Ref.1 Inferred from direct assay. Source: RGD
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Ref.1 Inferred from direct assay. Source: RGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Sh3rf1	Q71F54	1	EBI-957514,EBI-957526

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 282

282

E3 ubiquitin-protein ligase SIAH1

PRO_0000056166

Regions

Zinc finger

41 – 76

RING-type

Zinc finger

93 – 153

SIAH-type

Region

90 – 282

193

SBD

Sites

Metal binding

Zinc 1 By similarity

Metal binding

105

Zinc 1 By similarity

Metal binding

117

Zinc 1 By similarity

Metal binding

121

Zinc 1 By similarity

Metal binding

128

Zinc 2 By similarity

Metal binding

135

Zinc 2 By similarity

Metal binding

147

Zinc 2 By similarity

Metal binding

152

Zinc 2 By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q920M9-1 [UniParc].

Last modified April 26, 2004. Version 2.
Checksum: 852EADC5DD4A4FFA
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FASTA

282

31,137

        10         20         30         40         50         60 
MSRQTATALP TGTSKCPPSQ RVPALTGTTA SNNDLASLFE CPVCFDYVLP PILQCQSGHL 

        70         80         90        100        110        120 
VCSNCRPKLT CCPTCRGPLG SIRNLAMEKV ANSVLFPCKY ASSGCEITLP HTEKAEHEEL 

       130        140        150        160        170        180 
CEFRPYSCPC PGASCKWQGS LDAVMPHLMH QHKSITTLQG EDIVFLATDI NLPGAVDWVM 

       190        200        210        220        230        240 
MQSCFGFHFM LVLEKQEKYD GHQQFFAIVQ LIGTRKQAEN FAYRLELNGH RRRLTWEATP 

       250        260        270        280 
RSIHEGIATA IMNSDCLVFD TSIAQLFAEN GNLGINVTIS MC

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References

[1]	"Regulation of synaptophysin degradation by mammalian homologues of Seven in Absentia." Wheeler T.C., Chin L.-S., Li Y., Roudabush F.L., Li L. J. Biol. Chem. 277:10273-10282(2002) [PubMed: 11786535] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN DEGRADATION OF SYP, SUBCELLULAR LOCATION, INTERACTION WITH UBE2E2. Strain: Sprague-Dawley.
[2]	"Rat Siah1A." Yamaguchi A., Hori O., Tohyama M. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Competitive interaction of seven in absentia homolog-1A and Ca2+/calmodulin with the cytoplasmic tail of group 1 metabotropic glutamate receptors." Ishikawa K., Nash S.R., Nishimune A., Neki A., Kaneko S., Nakanishi S. Genes Cells 4:381-390(1999) [PubMed: 10469171] [Abstract] Cited for: INTERACTION WITH GRM1 AND GRM5.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF389476 mRNA. Translation: AAL91362.1. AB067814 mRNA. Translation: BAB70753.1. Different initiation.
IPI	IPI00200708.
RefSeq	NP_543181.2.
UniGene	Rn.73937
3D structure databases
SMR	Q920M9. Positions 92-282.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q920M9. 1 interaction.
STRING	Q920M9.
Genome annotation databases
Ensembl	ENSRNOT00000020329; ENSRNOP00000020329; ENSRNOG00000015143; Rattus norvegicus. [Genome view]
GeneID	140941.
KEGG	rno:140941.
UCSC	NM_080905. rat.
Organism-specific databases
CTD	140941.
RGD	620449. Siah1a.
Phylogenomic databases
HOVERGEN	Q920M9.
OMA	TEMSRQT.
Gene expression databases
ArrayExpress	Q920M9.
Genevestigator	Q920M9.
GermOnline	ENSRNOG00000015143. Rattus norvegicus.
Family and domain databases
InterPro	IPR004162. 7-in-absentia-prot. IPR018121. 7-in-absentia-prot_TRAF-dom. IPR013323. SIAH-type. IPR013322. TRAF-type. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. IPR013010. Znf_SIAH. [Graphical view]
Gene3D	G3DSA:3.90.890.10. SIAH-type. 1 hit. G3DSA:2.60.210.10. TRAF-type. 1 hit.
PANTHER	PTHR10315. Sina. 1 hit.
Pfam	PF03145. Sina. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. PS51081. ZF_SIAH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620838.

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Entry information

Entry name

SIAH1_RAT

Accession

Primary (citable) accession number: Q920M9
Secondary accession number(s): Q06984

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents