Reviewed, UniProtKB/Swiss-Prot Q920L2 (DHSA_RAT)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Flavoprotein subunit of complex II
      Short name=Fp

Gene names

Name:

Sdha

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	FAD By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix sideBy similarity.
Miscellaneous	The complex, present in mitochondria, can be degraded to form EC 1.3.99.1, which no longer reacts with ubiquinone.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion By similarity
	Chain	36 – 656	621	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial		PRO_0000010338
Regions
	Nucleotide binding	83 – 98	16	FAD By similarity
Sites
	Active site	332	1	Proton acceptor By similarity
	Binding site	288	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity
	Binding site	399	1	Substrate By similarity
	Binding site	443	1	Substrate By similarity
Amino acid modifications
	Modified residue	91	1	Tele-8alpha-FAD histidine By similarity
	Modified residue	171	1	N6-acetyllysine By similarity
	Modified residue	244	1	Phosphothreonine By similarity
	Modified residue	415	1	N6-acetyllysine By similarity
	Modified residue	477	1	N6-acetyllysine By similarity
	Modified residue	490	1	N6-acetyllysine By similarity
	Modified residue	530	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q920L2-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9E820F7FF7EC3B36	FASTA	656	71,615
	10 20 30 40 50 60 MAGVGAVSRL LRGRRLALAG ATRGFHFSVG ESKKASAKVS DAISTQYPVV DHEFDAVVVG 70 80 90 100 110 120 AGGAGLRAAF GLSEAGFNTA CLTKLFPTRS HTVAAQGGIN AALGNMEEDN WRWHFYDTVK 130 140 150 160 170 180 GSDWLGDQDA IHYMTEQAPA SVVELENYGM PFSRTEDGRI YQRAFGGQSL KFGKGGQAHR 190 200 210 220 230 240 CCCVADRTGH SLLHTLYGRS LRYDTSYFVE YFALDLLMEN GECRGVIALC IEDGSIHRIR 250 260 270 280 290 300 AKNTIIATGG YGRTYFSCTS AHTSTGDGTA MVTRAGLPCQ DLEFVQFHPT GIYGAGCLIT 310 320 330 340 350 360 EGCRGEGGIL INSQGERFME RYAPVAKDLA SRDVVSRSMT LEIREGRGCG PEKDHVYLQL 370 380 390 400 410 420 HHLPPEQLAT RLPGISETAM IFAGVDVTKE PIPVLPTVHY NMGGIPTNYK GQVLKHVNGQ 430 440 450 460 470 480 DQIVPGLYAC GEAACASVHG ANRLGANSLL DLVVFGRACA LSIAESCRPG DKVPPIKANA 490 500 510 520 530 540 GEESVMNLDK LRFADGSVRT SELRLSMQKS MQSHAAVFRV GSVLQEGCEK VSQLYGDLQH 550 560 570 580 590 600 LKTFDRGMVW NTDLVETLEL QNLMLCALQT IYGAEARKES RGAHAREDYK VRIDEYDYSK 610 620 630 640 650 PIEGQQKKPF AEHWRKHTLS YVDTKTGKVT LDYRPVIDKT LNEADCATVP PAIRSY

References

[1]	"Complex II from rat mitochondria." Tomitsuka E., Kita K. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 39-67; 225-238; 354-371 AND 444-457, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB072907 mRNA. Translation: BAB69818.1.
RefSeq	NP_569112.1.
UniGene	Rn.101725
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
SMR	Q920L2. Positions 45-656.
ModBase	Search...
PTM databases
PhosphoSite	Q920L2.
Genome annotation databases
Ensembl	ENSRNOG00000013331. Rattus norvegicus. [Contig view]
GeneID	157074.
KEGG	rno:157074.
NMPDR	fig|10116.3.peg.337.
Organism-specific databases
RGD	621557. Sdha.
Phylogenomic databases
HOVERGEN	Q920L2.
Gene expression databases
ArrayExpress	Q920L2.
GermOnline	ENSRNOG00000013331. Rattus norvegicus.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR001100. Pyr_nuc-diS_OxRdtase. IPR011281. Succ_DHase_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620967.

Entry information

Entry name

DHSA_RAT

Accession

Primary (citable) accession number: Q920L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	December 1, 2001
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)