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Q920L2 (SDHA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

EC=1.3.5.1
Alternative name(s):
Flavoprotein subunit of complex II
Short name=Fp
Gene names
Name:Sdha
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity. Can act as a tumor suppressor By similarity.

Catalytic activity

Succinate + a quinone = fumarate + a quinol.

Cofactor

FAD By similarity.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.

Subunit structure

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interacts with SDHAF2/SDH5; interaction is required for FAD attachment By similarity. Interacts with TRAP1 By similarity.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.

Post-translational modification

Phosphorylation at Tyr-207 is important for efficient electron transfer in complex II and the prevention of ROS generation By similarity.

Deacetylated by SIRT3 By similarity.

Sequence similarities

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3535Mitochondrion By similarity
Chain36 – 656621Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
PRO_0000010338

Regions

Nucleotide binding60 – 656FAD By similarity
Nucleotide binding83 – 9816FAD By similarity
Nucleotide binding448 – 4492FAD By similarity

Sites

Active site3321Proton acceptor By similarity
Binding site2671FAD By similarity
Binding site2881Substrate By similarity
Binding site3001Substrate By similarity
Binding site3991Substrate By similarity
Binding site4321FAD By similarity
Binding site4431Substrate By similarity

Amino acid modifications

Modified residue911Tele-8alpha-FAD histidine By similarity
Modified residue1711N6-acetyllysine; alternate By similarity
Modified residue1711N6-succinyllysine; alternate By similarity
Modified residue1741N6-acetyllysine By similarity
Modified residue2071Phosphotyrosine; by SRC By similarity
Modified residue2421N6-acetyllysine; alternate By similarity
Modified residue2421N6-succinyllysine; alternate By similarity
Modified residue3271N6-acetyllysine; alternate By similarity
Modified residue3271N6-succinyllysine; alternate By similarity
Modified residue4151N6-acetyllysine By similarity
Modified residue4721N6-acetyllysine By similarity
Modified residue4771N6-acetyllysine; alternate By similarity
Modified residue4771N6-succinyllysine; alternate By similarity
Modified residue4901N6-acetyllysine; alternate By similarity
Modified residue4901N6-succinyllysine; alternate By similarity
Modified residue5091N6-acetyllysine By similarity
Modified residue5301N6-acetyllysine; alternate By similarity
Modified residue5301N6-succinyllysine; alternate By similarity
Modified residue5421N6-acetyllysine By similarity
Modified residue5901N6-acetyllysine By similarity
Modified residue6001N6-acetyllysine By similarity
Modified residue6071N6-succinyllysine By similarity
Modified residue6161N6-acetyllysine By similarity
Modified residue6251N6-acetyllysine By similarity
Modified residue6281N6-acetyllysine By similarity
Modified residue6391N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q920L2 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 9E820F7FF7EC3B36

FASTA65671,615
        10         20         30         40         50         60 
MAGVGAVSRL LRGRRLALAG ATRGFHFSVG ESKKASAKVS DAISTQYPVV DHEFDAVVVG 

        70         80         90        100        110        120 
AGGAGLRAAF GLSEAGFNTA CLTKLFPTRS HTVAAQGGIN AALGNMEEDN WRWHFYDTVK 

       130        140        150        160        170        180 
GSDWLGDQDA IHYMTEQAPA SVVELENYGM PFSRTEDGRI YQRAFGGQSL KFGKGGQAHR 

       190        200        210        220        230        240 
CCCVADRTGH SLLHTLYGRS LRYDTSYFVE YFALDLLMEN GECRGVIALC IEDGSIHRIR 

       250        260        270        280        290        300 
AKNTIIATGG YGRTYFSCTS AHTSTGDGTA MVTRAGLPCQ DLEFVQFHPT GIYGAGCLIT 

       310        320        330        340        350        360 
EGCRGEGGIL INSQGERFME RYAPVAKDLA SRDVVSRSMT LEIREGRGCG PEKDHVYLQL 

       370        380        390        400        410        420 
HHLPPEQLAT RLPGISETAM IFAGVDVTKE PIPVLPTVHY NMGGIPTNYK GQVLKHVNGQ 

       430        440        450        460        470        480 
DQIVPGLYAC GEAACASVHG ANRLGANSLL DLVVFGRACA LSIAESCRPG DKVPPIKANA 

       490        500        510        520        530        540 
GEESVMNLDK LRFADGSVRT SELRLSMQKS MQSHAAVFRV GSVLQEGCEK VSQLYGDLQH 

       550        560        570        580        590        600 
LKTFDRGMVW NTDLVETLEL QNLMLCALQT IYGAEARKES RGAHAREDYK VRIDEYDYSK 

       610        620        630        640        650 
PIEGQQKKPF AEHWRKHTLS YVDTKTGKVT LDYRPVIDKT LNEADCATVP PAIRSY 

« Hide

References

[1]"Complex II from rat mitochondria."
Tomitsuka E., Kita K.
Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lubec G., Afjehi-Sadat L.
Submitted (NOV-2006) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 39-67; 225-238; 354-371 AND 444-457, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB072907 mRNA. Translation: BAB69818.1.
RefSeqNP_569112.1. NM_130428.1.
UniGeneRn.228607.

3D structure databases

ProteinModelPortalQ920L2.
SMRQ920L2. Positions 44-656.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ920L2. 1 interaction.

PTM databases

PhosphoSiteQ920L2.

2D gel databases

World-2DPAGE0004:Q920L2.

Proteomic databases

PaxDbQ920L2.
PRIDEQ920L2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018336; ENSRNOP00000018336; ENSRNOG00000013331.
GeneID157074.
KEGGrno:157074.
UCSCRGD:621557. rat.

Organism-specific databases

CTD6389.
RGD621557. Sdha.

Phylogenomic databases

eggNOGCOG1053.
GeneTreeENSGT00390000010046.
HOGENOMHOG000160475.
HOVERGENHBG001461.
KOK00234.
OMARDGPILQ.
OrthoDBEOG7MH0XJ.
PhylomeDBQ920L2.

Enzyme and pathway databases

SABIO-RKQ920L2.
UniPathwayUPA00223; UER01006.

Gene expression databases

GenevestigatorQ920L2.

Family and domain databases

Gene3D1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio620967.
PROQ920L2.

Entry information

Entry nameSDHA_RAT
AccessionPrimary (citable) accession number: Q920L2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: December 1, 2001
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways