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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

Sdha

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei267 – 2671FADBy similarity
Binding sitei288 – 2881SubstrateBy similarity
Binding sitei300 – 3001SubstrateBy similarity
Active sitei332 – 3321Proton acceptorBy similarity
Binding sitei399 – 3991SubstrateBy similarity
Binding sitei432 – 4321FADBy similarity
Binding sitei443 – 4431SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi60 – 656FADBy similarity
Nucleotide bindingi83 – 9816FADBy similarityAdd
BLAST
Nucleotide bindingi448 – 4492FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. succinate dehydrogenase (ubiquinone) activity Source: RGD

GO - Biological processi

  1. nervous system development Source: Ensembl
  2. respiratory electron transport chain Source: UniProtKB
  3. succinate metabolic process Source: UniProtKB
  4. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_216424. Citric acid cycle (TCA cycle).
REACT_262146. Respiratory electron transport.
SABIO-RKQ920L2.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
Fp
Gene namesi
Name:Sdha
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi621557. Sdha.

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial respiratory chain complex II Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionBy similarityAdd
BLAST
Chaini36 – 656621Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialPRO_0000010338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei91 – 911Tele-8alpha-FAD histidineBy similarity
Modified residuei171 – 1711N6-acetyllysine; alternateBy similarity
Modified residuei171 – 1711N6-succinyllysine; alternateBy similarity
Modified residuei174 – 1741N6-acetyllysineBy similarity
Modified residuei207 – 2071Phosphotyrosine; by SRCBy similarity
Modified residuei242 – 2421N6-acetyllysine; alternateBy similarity
Modified residuei242 – 2421N6-succinyllysine; alternateBy similarity
Modified residuei327 – 3271N6-acetyllysine; alternateBy similarity
Modified residuei327 – 3271N6-succinyllysine; alternateBy similarity
Modified residuei415 – 4151N6-acetyllysineBy similarity
Modified residuei472 – 4721N6-acetyllysineBy similarity
Modified residuei477 – 4771N6-acetyllysine; alternateBy similarity
Modified residuei477 – 4771N6-succinyllysine; alternateBy similarity
Modified residuei490 – 4901N6-acetyllysine; alternateBy similarity
Modified residuei490 – 4901N6-succinyllysine; alternateBy similarity
Modified residuei509 – 5091N6-acetyllysineBy similarity
Modified residuei530 – 5301N6-acetyllysine; alternateBy similarity
Modified residuei530 – 5301N6-succinyllysine; alternateBy similarity
Modified residuei542 – 5421N6-acetyllysineBy similarity
Modified residuei590 – 5901N6-acetyllysineBy similarity
Modified residuei600 – 6001N6-acetyllysineBy similarity
Modified residuei607 – 6071N6-succinyllysineBy similarity
Modified residuei616 – 6161N6-acetyllysineBy similarity
Modified residuei625 – 6251N6-acetyllysineBy similarity
Modified residuei628 – 6281N6-acetyllysineBy similarity
Modified residuei639 – 6391N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylation at Tyr-207 is important for efficient electron transfer in complex II and the prevention of ROS generation.By similarity
Deacetylated by SIRT3.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ920L2.
PRIDEiQ920L2.

2D gel databases

World-2DPAGE0004:Q920L2.

PTM databases

PhosphoSiteiQ920L2.

Expressioni

Gene expression databases

ExpressionAtlasiQ920L2. baseline and differential.
GenevestigatoriQ920L2.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interacts with SDHAF2/SDH5; interaction is required for FAD attachment (By similarity). Interacts with TRAP1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ920L2. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ920L2.
SMRiQ920L2. Positions 44-656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1053.
GeneTreeiENSGT00390000010046.
HOGENOMiHOG000160475.
HOVERGENiHBG001461.
InParanoidiQ920L2.
KOiK00234.
OMAiKWRTCFT.
OrthoDBiEOG7MH0XJ.
PhylomeDBiQ920L2.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q920L2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVGAVSRL LRGRRLALAG ATRGFHFSVG ESKKASAKVS DAISTQYPVV
60 70 80 90 100
DHEFDAVVVG AGGAGLRAAF GLSEAGFNTA CLTKLFPTRS HTVAAQGGIN
110 120 130 140 150
AALGNMEEDN WRWHFYDTVK GSDWLGDQDA IHYMTEQAPA SVVELENYGM
160 170 180 190 200
PFSRTEDGRI YQRAFGGQSL KFGKGGQAHR CCCVADRTGH SLLHTLYGRS
210 220 230 240 250
LRYDTSYFVE YFALDLLMEN GECRGVIALC IEDGSIHRIR AKNTIIATGG
260 270 280 290 300
YGRTYFSCTS AHTSTGDGTA MVTRAGLPCQ DLEFVQFHPT GIYGAGCLIT
310 320 330 340 350
EGCRGEGGIL INSQGERFME RYAPVAKDLA SRDVVSRSMT LEIREGRGCG
360 370 380 390 400
PEKDHVYLQL HHLPPEQLAT RLPGISETAM IFAGVDVTKE PIPVLPTVHY
410 420 430 440 450
NMGGIPTNYK GQVLKHVNGQ DQIVPGLYAC GEAACASVHG ANRLGANSLL
460 470 480 490 500
DLVVFGRACA LSIAESCRPG DKVPPIKANA GEESVMNLDK LRFADGSVRT
510 520 530 540 550
SELRLSMQKS MQSHAAVFRV GSVLQEGCEK VSQLYGDLQH LKTFDRGMVW
560 570 580 590 600
NTDLVETLEL QNLMLCALQT IYGAEARKES RGAHAREDYK VRIDEYDYSK
610 620 630 640 650
PIEGQQKKPF AEHWRKHTLS YVDTKTGKVT LDYRPVIDKT LNEADCATVP

PAIRSY
Length:656
Mass (Da):71,615
Last modified:December 1, 2001 - v1
Checksum:i9E820F7FF7EC3B36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072907 mRNA. Translation: BAB69818.1.
RefSeqiNP_569112.1. NM_130428.1.
UniGeneiRn.228607.

Genome annotation databases

EnsembliENSRNOT00000018336; ENSRNOP00000018336; ENSRNOG00000013331.
GeneIDi157074.
KEGGirno:157074.
UCSCiRGD:621557. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072907 mRNA. Translation: BAB69818.1.
RefSeqiNP_569112.1. NM_130428.1.
UniGeneiRn.228607.

3D structure databases

ProteinModelPortaliQ920L2.
SMRiQ920L2. Positions 44-656.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ920L2. 1 interaction.

PTM databases

PhosphoSiteiQ920L2.

2D gel databases

World-2DPAGE0004:Q920L2.

Proteomic databases

PaxDbiQ920L2.
PRIDEiQ920L2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018336; ENSRNOP00000018336; ENSRNOG00000013331.
GeneIDi157074.
KEGGirno:157074.
UCSCiRGD:621557. rat.

Organism-specific databases

CTDi6389.
RGDi621557. Sdha.

Phylogenomic databases

eggNOGiCOG1053.
GeneTreeiENSGT00390000010046.
HOGENOMiHOG000160475.
HOVERGENiHBG001461.
InParanoidiQ920L2.
KOiK00234.
OMAiKWRTCFT.
OrthoDBiEOG7MH0XJ.
PhylomeDBiQ920L2.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
ReactomeiREACT_216424. Citric acid cycle (TCA cycle).
REACT_262146. Respiratory electron transport.
SABIO-RKQ920L2.

Miscellaneous databases

NextBioi620967.
PROiQ920L2.

Gene expression databases

ExpressionAtlasiQ920L2. baseline and differential.
GenevestigatoriQ920L2.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complex II from rat mitochondria."
    Tomitsuka E., Kita K.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 39-67; 225-238; 354-371 AND 444-457, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.

Entry informationi

Entry nameiSDHA_RAT
AccessioniPrimary (citable) accession number: Q920L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: December 1, 2001
Last modified: March 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.