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Protein

Hephaestin

Gene

Heph

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1 (By similarity).By similarity

Cofactori

Cu cationBy similarityNote: Binds 6 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi126Copper 1; type 2By similarity1
Metal bindingi128Copper 2; type 3By similarity1
Metal bindingi186Copper 2; type 3By similarity1
Metal bindingi188Copper 3; type 3By similarity1
Metal bindingi304Copper 4; type 1By similarity1
Metal bindingi347Copper 4; type 1By similarity1
Metal bindingi352Copper 4; type 1By similarity1
Metal bindingi655Copper 5; type 1By similarity1
Metal bindingi698Copper 5; type 1By similarity1
Metal bindingi703Copper 5; type 1By similarity1
Metal bindingi708Copper 5; type 1By similarity1
Metal bindingi999Copper 6; type 1By similarity1
Metal bindingi1002Copper 1; type 2By similarity1
Metal bindingi1004Copper 3; type 3By similarity1
Metal bindingi1044Copper 3; type 3By similarity1
Metal bindingi1045Copper 6; type 1By similarity1
Metal bindingi1046Copper 2; type 3By similarity1
Metal bindingi1050Copper 6; type 1By similarity1
Metal bindingi1055Copper 6; type 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Copper transport, Ion transport, Iron transport, Transport

Keywords - Ligandi

Copper, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-425410. Metal ion SLC transporters.
R-RNO-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Hephaestin (EC:1.-.-.-)
Gene namesi
Name:Heph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi71060. Heph.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 1109ExtracellularSequence analysisAdd BLAST1086
Transmembranei1110 – 1130HelicalSequence analysisAdd BLAST21
Topological domaini1131 – 1157CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

  • basolateral plasma membrane Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000291724 – 1157HephaestinAdd BLAST1134

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi49N-linked (GlcNAc...)Sequence analysis1
Glycosylationi54N-linked (GlcNAc...)Sequence analysis1
Glycosylationi164N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi180 ↔ 206Sequence analysis
Glycosylationi236N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi285 ↔ 366Sequence analysis
Disulfide bondi533 ↔ 559Sequence analysis
Glycosylationi587N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi636 ↔ 717Sequence analysis
Glycosylationi713N-linked (GlcNAc...)Sequence analysis1
Glycosylationi757N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi876 ↔ 902Sequence analysis
Glycosylationi930N-linked (GlcNAc...)Sequence analysis1
Modified residuei1144PhosphoserineCombined sources1
Modified residuei1149PhosphoserineCombined sources1
Modified residuei1154PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ920H8.
PRIDEiQ920H8.

PTM databases

iPTMnetiQ920H8.
PhosphoSitePlusiQ920H8.

Expressioni

Tissue specificityi

Highly expressed in small intestine and colon.1 Publication

Gene expression databases

BgeeiENSRNOG00000012294.
GenevisibleiQ920H8. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017312.

Structurei

3D structure databases

ProteinModelPortaliQ920H8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 206Plastocyanin-like 1Add BLAST183
Domaini218 – 366Plastocyanin-like 2Add BLAST149
Domaini379 – 559Plastocyanin-like 3Add BLAST181
Domaini569 – 717Plastocyanin-like 4Add BLAST149
Domaini730 – 902Plastocyanin-like 5Add BLAST173
Domaini910 – 1086Plastocyanin-like 6Add BLAST177

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 6 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
GeneTreeiENSGT00550000074552.
HOGENOMiHOG000231499.
HOVERGENiHBG003674.
InParanoidiQ920H8.
KOiK14735.
OMAiLIHLKNF.
PhylomeDBiQ920H8.
TreeFamiTF329807.

Family and domain databases

Gene3Di2.60.40.420. 6 hits.
InterProiIPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR027154. HEPH.
[Graphical view]
PANTHERiPTHR10127:SF622. PTHR10127:SF622. 1 hit.
PfamiPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 3 hits.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 6 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 3 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q920H8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAGHLLWAL LLMHSLCSLP TDGAIRNYYL GIQDIQWNYA PKGRNVITNQ
60 70 80 90 100
TLNNDTVASS FLKSGKNRIG GTYKKTVYKE YSDGTYTNEI AKPAWLGFLG
110 120 130 140 150
PLLKAEMGDV ILIHLKNFAS RPYTIHPHGV FYEKDSEGSL YPDGSSGYLK
160 170 180 190 200
ADDSVPPGGS HVYNWSIPEG HAPTEADPAC LTWIYHSHVD APRDIATGLI
210 220 230 240 250
GPLITCKRGT LDGNSPPQRK DVDHNFFLLF SVIDENLSWH LDDNIATYCS
260 270 280 290 300
DPASVDKEDG PFQDSNRMHA INGFVFGNLP ELSMCAQKHV AWHLFGMGNE
310 320 330 340 350
IDVHTAFFHG QTLSIRGHRT DVAHIFPATF VTAEMVPQKS GTWLISCVVN
360 370 380 390 400
SHLKSGMQAF YKVDSCSMDP PVEQLTGKVR QYFIQAHEIQ WDYGPIGHDG
410 420 430 440 450
RTGKSLREPG SGPDKYFQKS SSRIGGTYWK VRYEAFQDET FQERLHQEEE
460 470 480 490 500
THLGILGPVI RAEVGDTIQV VFYNRASQPF SIQPHGVFYE KSSEGTVYND
510 520 530 540 550
GTSYPKVAKS FEKVTYYWTV PPHAGPTAED PACLTWMYFS AADPTRDTNS
560 570 580 590 600
GLVGPLLVCK AGALGEDGKQ KGVDKEFFLL FTIFDENESW YNNANQAAGM
610 620 630 640 650
LDSRLLSEDV EGFEDSNRMH AINGFLFSNL PRLDICKGDT VAWHLLGLGT
660 670 680 690 700
ENDVHGVMFE GNTLQLQGMR KSAAMLFPHT FVTAIMQPDN PGIFEIYCQA
710 720 730 740 750
GSHREAGMQA IYNVSQCSSH QDSPRQHYQA SRVYYIMAEE IEWDYCPDRS
760 770 780 790 800
WELEWYNTSE KDSYGHVFLS NKDGLLGSKY KKAVFREYTD GTFRIPQPRS
810 820 830 840 850
GPEEHLGILG PLIRGEVGDI LTVVFKNKAS RPYSIHAHGV LESSTGWPQA
860 870 880 890 900
AEPGEVLTYQ WNIPERSGPG PSDSACVSWI YYSAVDPIKD MYSGLVGPLV
910 920 930 940 950
ICRNGILEPN GGRNDMDREF ALLFLIFDEN QSWYLKENIA TYGPQETSHV
960 970 980 990 1000
NLQDATFLES NKMHAINGKL YANLRGLTVY QGERVAWYML AMGQDTDIHT
1010 1020 1030 1040 1050
VHFHAESFLY QNGHSYRADV VDLFPGTFEV VEMVASNPGA WLMHCHVTDH
1060 1070 1080 1090 1100
VHAGMETIFT VLSHEEHFST MTTITKEIGK AVILQNIGEG NVKMLGMNIP
1110 1120 1130 1140 1150
VKNVEILSSA LIAICVVLLL IALALGGVVW YQHRQRKLRR NRRSILDDSF

KLLSLKQ
Length:1,157
Mass (Da):129,593
Last modified:December 1, 2001 - v1
Checksum:i0C626FA3E2F51DE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246120 mRNA. Translation: AAL08217.1.
RefSeqiNP_579838.1. NM_133304.1.
XP_006257108.1. XM_006257046.3.
XP_006257110.1. XM_006257048.3.
XP_006257111.1. XM_006257049.3.
XP_008771470.1. XM_008773248.1.
XP_008771471.1. XM_008773249.2.
XP_008771472.1. XM_008773250.2.
UniGeneiRn.43849.

Genome annotation databases

EnsembliENSRNOT00000017312; ENSRNOP00000017312; ENSRNOG00000012294.
ENSRNOT00000092103; ENSRNOP00000070353; ENSRNOG00000012294.
GeneIDi117240.
KEGGirno:117240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246120 mRNA. Translation: AAL08217.1.
RefSeqiNP_579838.1. NM_133304.1.
XP_006257108.1. XM_006257046.3.
XP_006257110.1. XM_006257048.3.
XP_006257111.1. XM_006257049.3.
XP_008771470.1. XM_008773248.1.
XP_008771471.1. XM_008773249.2.
XP_008771472.1. XM_008773250.2.
UniGeneiRn.43849.

3D structure databases

ProteinModelPortaliQ920H8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017312.

PTM databases

iPTMnetiQ920H8.
PhosphoSitePlusiQ920H8.

Proteomic databases

PaxDbiQ920H8.
PRIDEiQ920H8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017312; ENSRNOP00000017312; ENSRNOG00000012294.
ENSRNOT00000092103; ENSRNOP00000070353; ENSRNOG00000012294.
GeneIDi117240.
KEGGirno:117240.

Organism-specific databases

CTDi9843.
RGDi71060. Heph.

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
GeneTreeiENSGT00550000074552.
HOGENOMiHOG000231499.
HOVERGENiHBG003674.
InParanoidiQ920H8.
KOiK14735.
OMAiLIHLKNF.
PhylomeDBiQ920H8.
TreeFamiTF329807.

Enzyme and pathway databases

ReactomeiR-RNO-425410. Metal ion SLC transporters.
R-RNO-917937. Iron uptake and transport.

Miscellaneous databases

PROiQ920H8.

Gene expression databases

BgeeiENSRNOG00000012294.
GenevisibleiQ920H8. RN.

Family and domain databases

Gene3Di2.60.40.420. 6 hits.
InterProiIPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR027154. HEPH.
[Graphical view]
PANTHERiPTHR10127:SF622. PTHR10127:SF622. 1 hit.
PfamiPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 3 hits.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 6 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 3 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEPH_RAT
AccessioniPrimary (citable) accession number: Q920H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.