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Protein

Hephaestin

Gene

Heph

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1 (By similarity).By similarity

Cofactori

Cu cationBy similarityNote: Binds 6 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi126 – 1261Copper 1; type 2By similarity
Metal bindingi128 – 1281Copper 2; type 3By similarity
Metal bindingi186 – 1861Copper 2; type 3By similarity
Metal bindingi188 – 1881Copper 3; type 3By similarity
Metal bindingi304 – 3041Copper 4; type 1By similarity
Metal bindingi347 – 3471Copper 4; type 1By similarity
Metal bindingi352 – 3521Copper 4; type 1By similarity
Metal bindingi655 – 6551Copper 5; type 1By similarity
Metal bindingi698 – 6981Copper 5; type 1By similarity
Metal bindingi703 – 7031Copper 5; type 1By similarity
Metal bindingi708 – 7081Copper 5; type 1By similarity
Metal bindingi999 – 9991Copper 6; type 1By similarity
Metal bindingi1002 – 10021Copper 1; type 2By similarity
Metal bindingi1004 – 10041Copper 3; type 3By similarity
Metal bindingi1044 – 10441Copper 3; type 3By similarity
Metal bindingi1045 – 10451Copper 6; type 1By similarity
Metal bindingi1046 – 10461Copper 2; type 3By similarity
Metal bindingi1050 – 10501Copper 6; type 1By similarity
Metal bindingi1055 – 10551Copper 6; type 1By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Copper transport, Ion transport, Iron transport, Transport

Keywords - Ligandi

Copper, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-425410. Metal ion SLC transporters.
R-RNO-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Hephaestin (EC:1.-.-.-)
Gene namesi
Name:Heph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi71060. Heph.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 11091086ExtracellularSequence analysisAdd
BLAST
Transmembranei1110 – 113021HelicalSequence analysisAdd
BLAST
Topological domaini1131 – 115727CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 11571134HephaestinPRO_0000002917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence analysis
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence analysis
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence analysis
Disulfide bondi180 ↔ 206Sequence analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence analysis
Disulfide bondi285 ↔ 366Sequence analysis
Disulfide bondi533 ↔ 559Sequence analysis
Glycosylationi587 – 5871N-linked (GlcNAc...)Sequence analysis
Disulfide bondi636 ↔ 717Sequence analysis
Glycosylationi713 – 7131N-linked (GlcNAc...)Sequence analysis
Glycosylationi757 – 7571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi876 ↔ 902Sequence analysis
Glycosylationi930 – 9301N-linked (GlcNAc...)Sequence analysis
Modified residuei1144 – 11441PhosphoserineCombined sources
Modified residuei1149 – 11491PhosphoserineCombined sources
Modified residuei1154 – 11541PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ920H8.
PRIDEiQ920H8.

PTM databases

iPTMnetiQ920H8.

Expressioni

Tissue specificityi

Highly expressed in small intestine and colon.1 Publication

Gene expression databases

GenevisibleiQ920H8. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017312.

Structurei

3D structure databases

ProteinModelPortaliQ920H8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 206183Plastocyanin-like 1Add
BLAST
Domaini218 – 366149Plastocyanin-like 2Add
BLAST
Domaini379 – 559181Plastocyanin-like 3Add
BLAST
Domaini569 – 717149Plastocyanin-like 4Add
BLAST
Domaini730 – 902173Plastocyanin-like 5Add
BLAST
Domaini910 – 1086177Plastocyanin-like 6Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 6 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
GeneTreeiENSGT00550000074552.
HOGENOMiHOG000231499.
HOVERGENiHBG003674.
InParanoidiQ920H8.
KOiK14735.
OMAiLIHLKNF.
OrthoDBiEOG7V49XN.
PhylomeDBiQ920H8.
TreeFamiTF329807.

Family and domain databases

Gene3Di2.60.40.420. 6 hits.
InterProiIPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR027154. HEPH.
[Graphical view]
PANTHERiPTHR10127:SF622. PTHR10127:SF622. 1 hit.
PfamiPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 3 hits.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 6 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 3 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q920H8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAGHLLWAL LLMHSLCSLP TDGAIRNYYL GIQDIQWNYA PKGRNVITNQ
60 70 80 90 100
TLNNDTVASS FLKSGKNRIG GTYKKTVYKE YSDGTYTNEI AKPAWLGFLG
110 120 130 140 150
PLLKAEMGDV ILIHLKNFAS RPYTIHPHGV FYEKDSEGSL YPDGSSGYLK
160 170 180 190 200
ADDSVPPGGS HVYNWSIPEG HAPTEADPAC LTWIYHSHVD APRDIATGLI
210 220 230 240 250
GPLITCKRGT LDGNSPPQRK DVDHNFFLLF SVIDENLSWH LDDNIATYCS
260 270 280 290 300
DPASVDKEDG PFQDSNRMHA INGFVFGNLP ELSMCAQKHV AWHLFGMGNE
310 320 330 340 350
IDVHTAFFHG QTLSIRGHRT DVAHIFPATF VTAEMVPQKS GTWLISCVVN
360 370 380 390 400
SHLKSGMQAF YKVDSCSMDP PVEQLTGKVR QYFIQAHEIQ WDYGPIGHDG
410 420 430 440 450
RTGKSLREPG SGPDKYFQKS SSRIGGTYWK VRYEAFQDET FQERLHQEEE
460 470 480 490 500
THLGILGPVI RAEVGDTIQV VFYNRASQPF SIQPHGVFYE KSSEGTVYND
510 520 530 540 550
GTSYPKVAKS FEKVTYYWTV PPHAGPTAED PACLTWMYFS AADPTRDTNS
560 570 580 590 600
GLVGPLLVCK AGALGEDGKQ KGVDKEFFLL FTIFDENESW YNNANQAAGM
610 620 630 640 650
LDSRLLSEDV EGFEDSNRMH AINGFLFSNL PRLDICKGDT VAWHLLGLGT
660 670 680 690 700
ENDVHGVMFE GNTLQLQGMR KSAAMLFPHT FVTAIMQPDN PGIFEIYCQA
710 720 730 740 750
GSHREAGMQA IYNVSQCSSH QDSPRQHYQA SRVYYIMAEE IEWDYCPDRS
760 770 780 790 800
WELEWYNTSE KDSYGHVFLS NKDGLLGSKY KKAVFREYTD GTFRIPQPRS
810 820 830 840 850
GPEEHLGILG PLIRGEVGDI LTVVFKNKAS RPYSIHAHGV LESSTGWPQA
860 870 880 890 900
AEPGEVLTYQ WNIPERSGPG PSDSACVSWI YYSAVDPIKD MYSGLVGPLV
910 920 930 940 950
ICRNGILEPN GGRNDMDREF ALLFLIFDEN QSWYLKENIA TYGPQETSHV
960 970 980 990 1000
NLQDATFLES NKMHAINGKL YANLRGLTVY QGERVAWYML AMGQDTDIHT
1010 1020 1030 1040 1050
VHFHAESFLY QNGHSYRADV VDLFPGTFEV VEMVASNPGA WLMHCHVTDH
1060 1070 1080 1090 1100
VHAGMETIFT VLSHEEHFST MTTITKEIGK AVILQNIGEG NVKMLGMNIP
1110 1120 1130 1140 1150
VKNVEILSSA LIAICVVLLL IALALGGVVW YQHRQRKLRR NRRSILDDSF

KLLSLKQ
Length:1,157
Mass (Da):129,593
Last modified:December 1, 2001 - v1
Checksum:i0C626FA3E2F51DE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246120 mRNA. Translation: AAL08217.1.
RefSeqiNP_579838.1. NM_133304.1.
XP_006257108.1. XM_006257046.2.
XP_006257110.1. XM_006257048.2.
XP_006257111.1. XM_006257049.2.
XP_008771470.1. XM_008773248.1.
XP_008771471.1. XM_008773249.1.
XP_008771472.1. XM_008773250.1.
UniGeneiRn.43849.

Genome annotation databases

EnsembliENSRNOT00000017312; ENSRNOP00000017312; ENSRNOG00000012294.
ENSRNOT00000092103; ENSRNOP00000070353; ENSRNOG00000012294.
GeneIDi117240.
KEGGirno:117240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF246120 mRNA. Translation: AAL08217.1.
RefSeqiNP_579838.1. NM_133304.1.
XP_006257108.1. XM_006257046.2.
XP_006257110.1. XM_006257048.2.
XP_006257111.1. XM_006257049.2.
XP_008771470.1. XM_008773248.1.
XP_008771471.1. XM_008773249.1.
XP_008771472.1. XM_008773250.1.
UniGeneiRn.43849.

3D structure databases

ProteinModelPortaliQ920H8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017312.

PTM databases

iPTMnetiQ920H8.

Proteomic databases

PaxDbiQ920H8.
PRIDEiQ920H8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017312; ENSRNOP00000017312; ENSRNOG00000012294.
ENSRNOT00000092103; ENSRNOP00000070353; ENSRNOG00000012294.
GeneIDi117240.
KEGGirno:117240.

Organism-specific databases

CTDi9843.
RGDi71060. Heph.

Phylogenomic databases

eggNOGiKOG1263. Eukaryota.
COG2132. LUCA.
GeneTreeiENSGT00550000074552.
HOGENOMiHOG000231499.
HOVERGENiHBG003674.
InParanoidiQ920H8.
KOiK14735.
OMAiLIHLKNF.
OrthoDBiEOG7V49XN.
PhylomeDBiQ920H8.
TreeFamiTF329807.

Enzyme and pathway databases

ReactomeiR-RNO-425410. Metal ion SLC transporters.
R-RNO-917937. Iron uptake and transport.

Miscellaneous databases

PROiQ920H8.

Gene expression databases

GenevisibleiQ920H8. RN.

Family and domain databases

Gene3Di2.60.40.420. 6 hits.
InterProiIPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR033138. Cu_oxidase_CS.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR027154. HEPH.
[Graphical view]
PANTHERiPTHR10127:SF622. PTHR10127:SF622. 1 hit.
PfamiPF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 3 hits.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 6 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 3 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins."
    Frazer D.M., Vulpe C.D., McKie A.T., Wilkins S.J., Trinder D., Cleghorn G.J., Anderson G.J.
    Am. J. Physiol. 281:G931-G939(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1144 AND SER-1149, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHEPH_RAT
AccessioniPrimary (citable) accession number: Q920H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.