Muscarinic acetylcholine receptor M5

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Q920H4 (ACM5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Muscarinic acetylcholine receptor M5

Gene names

Name:

Chrm5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dopamine transport Inferred from direct assay PubMed 11756520. Source: MGI regulation of phosphatidylinositol dephosphorylation Inferred from electronic annotation. Source: Compara transmission of nerve impulse Inferred from direct assay PubMed 11756520. Source: MGI
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled acetylcholine receptor activity Inferred from electronic annotation. Source: Compara extracellular-glycine-gated chloride channel activity Traceable author statement PubMed 11756520. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 532

532

Muscarinic acetylcholine receptor M5

PRO_0000069044

Regions

Topological domain

1 – 29

Extracellular By similarity

Transmembrane

30 – 53

Helical; Name=1; By similarity

Topological domain

54 – 66

Cytoplasmic By similarity

Transmembrane

67 – 87

Helical; Name=2; By similarity

Topological domain

88 – 104

Extracellular By similarity

Transmembrane

105 – 126

Helical; Name=3; By similarity

Topological domain

127 – 146

Cytoplasmic By similarity

Transmembrane

147 – 169

Helical; Name=4; By similarity

Topological domain

170 – 191

Extracellular By similarity

Transmembrane

192 – 214

Helical; Name=5; By similarity

Topological domain

215 – 443

229

Cytoplasmic By similarity

Transmembrane

444 – 464

Helical; Name=6; By similarity

Topological domain

465 – 478

Extracellular By similarity

Transmembrane

479 – 498

Helical; Name=7; By similarity

Topological domain

499 – 532

Cytoplasmic By similarity

Amino acid modifications

Modified residue

501

Phosphothreonine Potential

Modified residue

505

Phosphothreonine Potential

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

103 ↔ 183

By similarity

Experimental info

Sequence conflict

265

V → E in AAL26028. Ref.1

Sequence conflict

265

V → E in AAI20616. Ref.3

Sequence conflict

325

C → Y in AAL26028. Ref.1

Sequence conflict

325

C → Y in AAI20616. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q920H4 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 02AA5498F44C1DA6
Show »

FASTA

532

60,253

        10         20         30         40         50         60 
MEGESYHNET TVNGTPVNHQ ALERHGLWEV ITIAAVTAVV SLMTIVGNVL VMISFKVNSQ 

        70         80         90        100        110        120 
LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWVLGS LACDLWLALD YVASNASVMN 

       130        140        150        160        170        180 
LLVISFDRYF SITRPLTYRA KRTPKRAGIM IGLAWLVSFI LWAPAILCWQ YLVGKRTVPP 

       190        200        210        220        230        240 
DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVAEVK 

       250        260        270        280        290        300 
KRKPAHRTLL RSFFSCPRPS LAQRVRNQAS WSSSRRSTST TGKPTQATDL SADWEKAEQV 

       310        320        330        340        350        360 
TNCSSCPSSE DEAKATTDPV FQVVCKNEAK ESPGKEFNTQ ETKETFVSPR TENNDYDTPK 

       370        380        390        400        410        420 
YFLSPGAAHR LKSQKCVAYK FRLVVKADGT QETNNGCRKV KIMPCSFPVS KDPSTKGLDP 

       430        440        450        460        470        480 
HLSHQMTKRK RMVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG 

       490        500        510        520        530 
YWLCYVNSTI NPICYALCNR TFRKTFKLLL LCRWKKKKVE EKLYWQGNSK LP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, sequence and functional expression of the mouse M1-M5 muscarinic acetylcholine receptor genes." Yamada M., Wess J. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF264051 Genomic DNA. Translation: AAL26028.1. BC120615 mRNA. Translation: AAI20616.1. AL731840 Genomic DNA. Translation: CAM25807.1.
IPI	IPI00310390.
RefSeq	NP_991352.2. NM_205783.2.
UniGene	Mm.297624.
3D structure databases
ProteinModelPortal	Q920H4.
SMR	Q920H4. Positions 27-251, 439-507.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q920H4.
Proteomic databases
PRIDE	Q920H4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000099589; ENSMUSP00000097185; ENSMUSG00000074939.
GeneID	213788.
KEGG	mmu:213788.
Organism-specific databases
CTD	1133.
MGI	MGI:109248. Chrm5.
Phylogenomic databases
eggNOG	NOG250863.
GeneTree	ENSGT00690000101881.
HOGENOM	HOG000231484.
HOVERGEN	HBG105720.
InParanoid	A2AHU3.
KO	K04133.
OMA	RKPAHRA.
OrthoDB	EOG4H19VH.
Gene expression databases
Bgee	Q920H4.
CleanEx	MM_CHRM5.
Genevestigator	Q920H4.
GermOnline	ENSMUSG00000074939. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000502. Musac_M5_rcpt. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00542. MUSCRINICM5R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q920H4.
ChEMBL	CHEMBL2324.
NextBio	374108.
SOURCE	Search...

Entry information

Entry name

ACM5_MOUSE

Accession

Primary (citable) accession number: Q920H4
Secondary accession number(s): A2AHU3, Q0VBI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents