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Q920H4

- ACM5_MOUSE

UniProt

Q920H4 - ACM5_MOUSE

Protein

Muscarinic acetylcholine receptor M5

Gene

Chrm5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover By similarity.By similarity

    GO - Molecular functioni

    1. extracellular-glycine-gated chloride channel activity Source: MGI
    2. G-protein coupled acetylcholine receptor activity Source: Ensembl

    GO - Biological processi

    1. chloride transmembrane transport Source: GOC
    2. chloride transport Source: GOC
    3. dopamine transport Source: MGI
    4. gastric acid secretion Source: InterPro
    5. regulation of phosphatidylinositol dephosphorylation Source: Ensembl
    6. transmission of nerve impulse Source: MGI

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M5
    Gene namesi
    Name:Chrm5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:109248. Chrm5.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of plasma membrane Source: InterPro
    3. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 532532Muscarinic acetylcholine receptor M5PRO_0000069044Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi103 ↔ 183PROSITE-ProRule annotation
    Modified residuei501 – 5011PhosphothreonineSequence Analysis
    Modified residuei505 – 5051PhosphothreonineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ920H4.

    PTM databases

    PhosphoSiteiQ920H4.

    Expressioni

    Gene expression databases

    BgeeiQ920H4.
    CleanExiMM_CHRM5.
    GenevestigatoriQ920H4.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliQ920H4.
    SMRiQ920H4. Positions 27-240, 439-507.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2929ExtracellularBy similarityAdd
    BLAST
    Topological domaini54 – 6613CytoplasmicBy similarityAdd
    BLAST
    Topological domaini88 – 10417ExtracellularBy similarityAdd
    BLAST
    Topological domaini127 – 14620CytoplasmicBy similarityAdd
    BLAST
    Topological domaini170 – 19122ExtracellularBy similarityAdd
    BLAST
    Topological domaini215 – 443229CytoplasmicBy similarityAdd
    BLAST
    Topological domaini465 – 47814ExtracellularBy similarityAdd
    BLAST
    Topological domaini499 – 53234CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei30 – 5324Helical; Name=1By similarityAdd
    BLAST
    Transmembranei67 – 8721Helical; Name=2By similarityAdd
    BLAST
    Transmembranei105 – 12622Helical; Name=3By similarityAdd
    BLAST
    Transmembranei147 – 16923Helical; Name=4By similarityAdd
    BLAST
    Transmembranei192 – 21423Helical; Name=5By similarityAdd
    BLAST
    Transmembranei444 – 46421Helical; Name=6By similarityAdd
    BLAST
    Transmembranei479 – 49820Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    GeneTreeiENSGT00690000101881.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiA2AHU3.
    KOiK04133.
    OMAiYDTPKYF.
    OrthoDBiEOG7V49Z7.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000502. Musac_Ach_M5_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PANTHERiPTHR24249:SF58. PTHR24249:SF58. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00542. MUSCRINICM5R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q920H4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGESYHNET TVNGTPVNHQ ALERHGLWEV ITIAAVTAVV SLMTIVGNVL    50
    VMISFKVNSQ LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWVLGS 100
    LACDLWLALD YVASNASVMN LLVISFDRYF SITRPLTYRA KRTPKRAGIM 150
    IGLAWLVSFI LWAPAILCWQ YLVGKRTVPP DECQIQFLSE PTITFGTAIA 200
    AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVAEVK KRKPAHRTLL 250
    RSFFSCPRPS LAQRVRNQAS WSSSRRSTST TGKPTQATDL SADWEKAEQV 300
    TNCSSCPSSE DEAKATTDPV FQVVCKNEAK ESPGKEFNTQ ETKETFVSPR 350
    TENNDYDTPK YFLSPGAAHR LKSQKCVAYK FRLVVKADGT QETNNGCRKV 400
    KIMPCSFPVS KDPSTKGLDP HLSHQMTKRK RMVLVKERKA AQTLSAILLA 450
    FIITWTPYNI MVLVSTFCDK CVPVTLWHLG YWLCYVNSTI NPICYALCNR 500
    TFRKTFKLLL LCRWKKKKVE EKLYWQGNSK LP 532
    Length:532
    Mass (Da):60,253
    Last modified:July 27, 2011 - v2
    Checksum:i02AA5498F44C1DA6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti265 – 2651V → E in AAL26028. 1 PublicationCurated
    Sequence conflicti265 – 2651V → E in AAI20616. (PubMed:15489334)Curated
    Sequence conflicti325 – 3251C → Y in AAL26028. 1 PublicationCurated
    Sequence conflicti325 – 3251C → Y in AAI20616. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264051 Genomic DNA. Translation: AAL26028.1.
    BC120615 mRNA. Translation: AAI20616.1.
    AL731840 Genomic DNA. Translation: CAM25807.1.
    CCDSiCCDS16556.1.
    RefSeqiNP_991352.2. NM_205783.2.
    XP_006499162.1. XM_006499099.1.
    UniGeneiMm.297624.

    Genome annotation databases

    EnsembliENSMUST00000099589; ENSMUSP00000097185; ENSMUSG00000074939.
    GeneIDi213788.
    KEGGimmu:213788.
    UCSCiuc008lpd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264051 Genomic DNA. Translation: AAL26028.1 .
    BC120615 mRNA. Translation: AAI20616.1 .
    AL731840 Genomic DNA. Translation: CAM25807.1 .
    CCDSi CCDS16556.1.
    RefSeqi NP_991352.2. NM_205783.2.
    XP_006499162.1. XM_006499099.1.
    UniGenei Mm.297624.

    3D structure databases

    ProteinModelPortali Q920H4.
    SMRi Q920H4. Positions 27-240, 439-507.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q920H4.
    ChEMBLi CHEMBL2097162.
    GuidetoPHARMACOLOGYi 17.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q920H4.

    Proteomic databases

    PRIDEi Q920H4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000099589 ; ENSMUSP00000097185 ; ENSMUSG00000074939 .
    GeneIDi 213788.
    KEGGi mmu:213788.
    UCSCi uc008lpd.2. mouse.

    Organism-specific databases

    CTDi 1133.
    MGIi MGI:109248. Chrm5.

    Phylogenomic databases

    eggNOGi NOG250863.
    GeneTreei ENSGT00690000101881.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi A2AHU3.
    KOi K04133.
    OMAi YDTPKYF.
    OrthoDBi EOG7V49Z7.
    TreeFami TF320495.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    NextBioi 374108.
    PROi Q920H4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q920H4.
    CleanExi MM_CHRM5.
    Genevestigatori Q920H4.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR000502. Musac_Ach_M5_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    PANTHERi PTHR24249:SF58. PTHR24249:SF58. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00542. MUSCRINICM5R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequence and functional expression of the mouse M1-M5 muscarinic acetylcholine receptor genes."
      Yamada M., Wess J.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiACM5_MOUSE
    AccessioniPrimary (citable) accession number: Q920H4
    Secondary accession number(s): A2AHU3, Q0VBI9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3