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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Gene

Slc9a3r2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.1 Publication

GO - Molecular functioni

  • channel regulator activity Source: RGD
  • low-density lipoprotein particle receptor binding Source: RGD
  • PDZ domain binding Source: RGD
  • protein complex scaffold Source: RGD

GO - Biological processi

  • inner ear development Source: RGD
  • positive regulation of membrane potential Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Short name:
NHERF-2
Alternative name(s):
NHE3 kinase A regulatory protein E3KARP
SRY-interacting protein 1
Short name:
SIP-1
Sodium-hydrogen exchanger regulatory factor 2
Solute carrier family 9 isoform A3 regulatory factor 2
Tyrosine kinase activator protein 1
Short name:
TKA-1
Gene namesi
Name:Slc9a3r2
Synonyms:Nherf2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620380. Slc9a3r2.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • cytoplasm Source: RGD
  • endomembrane system Source: UniProtKB-SubCell
  • nucleus Source: RGD
  • plasma membrane Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Na(+)/H(+) exchange regulatory cofactor NHE-RF2PRO_0000096808Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei186 – 1861PhosphoserineCombined sources
Modified residuei254 – 2541PhosphoserineBy similarity
Modified residuei269 – 2691PhosphoserineBy similarity
Modified residuei280 – 2801PhosphoserineCombined sources
Modified residuei303 – 3031PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ920G2.
PRIDEiQ920G2.

PTM databases

iPTMnetiQ920G2.
PhosphoSiteiQ920G2.

Expressioni

Tissue specificityi

Glomerular epithelium cell (podocyte) (at protein level). Detected in liver, bile duct, kidney glomerulus and lung.2 Publications

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R1. Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX, PDZK1 and LPAR2 (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells. Interacts with SGK1 and KCNJ1/ROMK1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Asic3O352402EBI-982439,EBI-982374

GO - Molecular functioni

  • low-density lipoprotein particle receptor binding Source: RGD
  • PDZ domain binding Source: RGD
  • protein complex scaffold Source: RGD

Protein-protein interaction databases

IntActiQ920G2. 1 interaction.
STRINGi10116.ENSRNOP00000004172.

Structurei

3D structure databases

ProteinModelPortaliQ920G2.
SMRiQ920G2. Positions 9-91, 147-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 9181PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini151 – 23181PDZ 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITAC. Eukaryota.
ENOG4111V58. LUCA.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ920G2.
KOiK13358.
PhylomeDBiQ920G2.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR017300. NHERF-1/NHERF-2.
IPR001478. PDZ.
[Graphical view]
PfamiPF09007. EBP50_C. 2 hits.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q920G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL
60 70 80 90 100
RAGDRLVEVN GVNVEGETHH QVVHRIKAVE GQTQLLVVDK ETDEELCRRQ
110 120 130 140 150
LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGHKDV NGPPRELRPR
160 170 180 190 200
LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASLSGL RAQDRLIEVN
210 220 230 240 250
GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEDHVEG
260 270 280 290 300
PLPSPVTNGT SLAQLNGGSV CSSRSDLPGS EKDNEDGSAW KRDPFQESGL
310 320 330
HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF
Length:337
Mass (Da):37,369
Last modified:December 1, 2001 - v1
Checksum:iC4B8E6D0CCFDBB0E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741H → Q in AAK49392 (PubMed:11124833).Curated
Sequence conflicti137 – 1371H → Q in AAK49392 (PubMed:11124833).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294257 mRNA. Translation: AAK97088.1.
AF259898 mRNA. Translation: AAK49392.1.
RefSeqiNP_446263.2. NM_053811.3.
UniGeneiRn.39351.

Genome annotation databases

GeneIDi116501.
KEGGirno:116501.
UCSCiRGD:620380. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294257 mRNA. Translation: AAK97088.1.
AF259898 mRNA. Translation: AAK49392.1.
RefSeqiNP_446263.2. NM_053811.3.
UniGeneiRn.39351.

3D structure databases

ProteinModelPortaliQ920G2.
SMRiQ920G2. Positions 9-91, 147-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ920G2. 1 interaction.
STRINGi10116.ENSRNOP00000004172.

PTM databases

iPTMnetiQ920G2.
PhosphoSiteiQ920G2.

Proteomic databases

PaxDbiQ920G2.
PRIDEiQ920G2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116501.
KEGGirno:116501.
UCSCiRGD:620380. rat.

Organism-specific databases

CTDi9351.
RGDi620380. Slc9a3r2.

Phylogenomic databases

eggNOGiENOG410ITAC. Eukaryota.
ENOG4111V58. LUCA.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ920G2.
KOiK13358.
PhylomeDBiQ920G2.

Miscellaneous databases

PROiQ920G2.

Family and domain databases

Gene3Di2.30.42.10. 2 hits.
InterProiIPR015098. EBP50_C-term.
IPR017300. NHERF-1/NHERF-2.
IPR001478. PDZ.
[Graphical view]
PfamiPF09007. EBP50_C. 2 hits.
PF00595. PDZ. 2 hits.
[Graphical view]
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiPD283022. EBP50_C-term. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00228. PDZ. 2 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiPS50106. PDZ. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNHRF2_RAT
AccessioniPrimary (citable) accession number: Q920G2
Secondary accession number(s): Q91Y70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.