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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF2

Gene

Slc9a3r2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.1 Publication

GO - Molecular functioni

  • channel regulator activity Source: RGD
  • low-density lipoprotein particle receptor binding Source: RGD
  • PDZ domain binding Source: RGD
  • protein complex scaffold activity Source: RGD

GO - Biological processi

  • inner ear development Source: RGD
  • positive regulation of membrane potential Source: RGD

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF2
Short name:
NHERF-2
Alternative name(s):
NHE3 kinase A regulatory protein E3KARP
SRY-interacting protein 1
Short name:
SIP-1
Sodium-hydrogen exchanger regulatory factor 2
Solute carrier family 9 isoform A3 regulatory factor 2
Tyrosine kinase activator protein 1
Short name:
TKA-1
Gene namesi
Name:Slc9a3r2
Synonyms:Nherf2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620380. Slc9a3r2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968081 – 337Na(+)/H(+) exchange regulatory cofactor NHE-RF2Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineBy similarity1
Modified residuei183PhosphoserineBy similarity1
Modified residuei186PhosphoserineCombined sources1
Modified residuei254PhosphoserineBy similarity1
Modified residuei269PhosphoserineBy similarity1
Modified residuei280PhosphoserineCombined sources1
Modified residuei303PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ920G2.
PRIDEiQ920G2.

PTM databases

iPTMnetiQ920G2.
PhosphoSitePlusiQ920G2.

Expressioni

Tissue specificityi

Glomerular epithelium cell (podocyte) (at protein level). Detected in liver, bile duct, kidney glomerulus and lung.2 Publications

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R1. Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX, PDZK1 and LPAR2 (By similarity). Found in a complex with EZR, PODXL and SLC9A3R2. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells. Interacts with SGK1 and KCNJ1/ROMK1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Asic3O352402EBI-982439,EBI-982374

GO - Molecular functioni

  • low-density lipoprotein particle receptor binding Source: RGD
  • PDZ domain binding Source: RGD
  • protein complex scaffold activity Source: RGD

Protein-protein interaction databases

BioGridi250471. 1 interactor.
CORUMiQ920G2.
IntActiQ920G2. 1 interactor.
STRINGi10116.ENSRNOP00000004172.

Structurei

3D structure databases

ProteinModelPortaliQ920G2.
SMRiQ920G2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 91PDZ 1PROSITE-ProRule annotationAdd BLAST81
Domaini151 – 231PDZ 2PROSITE-ProRule annotationAdd BLAST81

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITAC. Eukaryota.
ENOG4111V58. LUCA.
HOGENOMiHOG000089940.
HOVERGENiHBG052616.
InParanoidiQ920G2.
KOiK13358.
PhylomeDBiQ920G2.

Family and domain databases

InterProiView protein in InterPro
IPR015098. EBP50_C-term.
IPR017300. NHERF-1/NHERF-2.
IPR031197. NHERF-2.
IPR001478. PDZ.
PANTHERiPTHR14191:SF11. PTHR14191:SF11. 1 hit.
PfamiView protein in Pfam
PF09007. EBP50_C. 2 hits.
PF00595. PDZ. 2 hits.
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD283022. EBP50_C-term. 1 hit.
SMARTiView protein in SMART
SM00228. PDZ. 2 hits.
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiView protein in PROSITE
PS50106. PDZ. 2 hits.

Sequencei

Sequence statusi: Complete.

Q920G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL
60 70 80 90 100
RAGDRLVEVN GVNVEGETHH QVVHRIKAVE GQTQLLVVDK ETDEELCRRQ
110 120 130 140 150
LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGHKDV NGPPRELRPR
160 170 180 190 200
LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASLSGL RAQDRLIEVN
210 220 230 240 250
GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEDHVEG
260 270 280 290 300
PLPSPVTNGT SLAQLNGGSV CSSRSDLPGS EKDNEDGSAW KRDPFQESGL
310 320 330
HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF
Length:337
Mass (Da):37,369
Last modified:December 1, 2001 - v1
Checksum:iC4B8E6D0CCFDBB0E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74H → Q in AAK49392 (PubMed:11124833).Curated1
Sequence conflicti137H → Q in AAK49392 (PubMed:11124833).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294257 mRNA. Translation: AAK97088.1.
AF259898 mRNA. Translation: AAK49392.1.
RefSeqiNP_446263.2. NM_053811.3.
UniGeneiRn.39351.

Genome annotation databases

GeneIDi116501.
KEGGirno:116501.
UCSCiRGD:620380. rat.

Similar proteinsi

Entry informationi

Entry nameiNHRF2_RAT
AccessioniPrimary (citable) accession number: Q920G2
Secondary accession number(s): Q91Y70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: December 1, 2001
Last modified: September 27, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome