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Protein

KH domain-containing, RNA-binding, signal transduction-associated protein 2

Gene

Khdrbs2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Its phosphorylation by FYN inhibits its ability to regulate splice site selection. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. May function as an adapter protein for Src kinases during mitosis (By similarity). Binds both poly(A) and poly(U) homopolymers (By similarity). Phosphorylation by PTK6 inhibits its RNA-binding ability (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-8849468. PTK6 Regulates Proteins Involved in RNA Processing.

Names & Taxonomyi

Protein namesi
Recommended name:
KH domain-containing, RNA-binding, signal transduction-associated protein 2
Alternative name(s):
Sam68-like mammalian protein 1
Short name:
SLM-1
Short name:
rSLM-1
Gene namesi
Name:Khdrbs2
Synonyms:Slm1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi621738. Khdrbs2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349KH domain-containing, RNA-binding, signal transduction-associated protein 2PRO_0000308955Add
BLAST

Post-translational modificationi

Methylated.By similarity
Phosphorylated on tyrosine residues by FYN. Tyrosine phosphorylated by PTK6 and SRC (By similarity). Tyrosine phosphorylated by SRC during mitosis (By similarity).By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ920F3.
PRIDEiQ920F3.

PTM databases

iPTMnetiQ920F3.
PhosphoSiteiQ920F3.

Expressioni

Tissue specificityi

Expressed in the cortex, cerebellum, striatum, midbrain, brainstem and thalamus of the brain (at protein level). Expressed in neurons (at protein level). Expressed in brain and testis. Expressed in the dentate gyrus of the hippocampus.1 Publication

Gene expression databases

GenevisibleiQ920F3. RN.

Interactioni

Subunit structurei

Self-associates to form homooligomers. Interacts with SAFB, SFRS9 and YTHDC1. Found in a complex with KHDRBS1, KHDRBS2 and KHDRBS3. Interacts with RBMX (By similarity). Interacts with the SH3 domains of FYN and PLCG1 (By similarity). Interacts with the SH2 domains of FYN, GRAP2, PLCG1 and RASA1 (By similarity). Interacts with RBMX.By similarity2 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016579.

Structurei

3D structure databases

ProteinModelPortaliQ920F3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 13571KHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi211 – 26454Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the KHDRBS family.Curated
Contains 1 KH domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG1588. Eukaryota.
COG5176. LUCA.
GeneTreeiENSGT00550000074434.
HOGENOMiHOG000230771.
HOVERGENiHBG079164.
InParanoidiQ920F3.
KOiK17843.
OMAiPPIARGV.
OrthoDBiEOG75MVX3.
PhylomeDBiQ920F3.
TreeFamiTF314878.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032571. Qua1_dom.
IPR032335. Sam68-YY.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16274. Qua1. 1 hit.
PF16568. Sam68-YY. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q920F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEEKYLPEL MAEKDSLDPS FVHASRLLAE EIEKFQGSDG RKEDEEKKYL
60 70 80 90 100
DVISNKNIKL SERVLIPVKQ YPKFNFVGKL LGPRGNSLKR LQEETGAKMS
110 120 130 140 150
ILGKGSMRDK AKEEELRKSG EAKYAHLSDE LHVLIEVFAP PGEAYSRMSH
160 170 180 190 200
ALEEIKKFLV PDYNDEIRQE QLRELSYLNG SEESGRGRGI RGRGIRITPT
210 220 230 240 250
APSRGRGGAV PPPPPPGRGV LTPRGTTVTR GALPVPPVAR GVPTPRARGT
260 270 280 290 300
AAVPGYRAPP PPAPEAYEEY GYDDGYGGEY DDQTYEAYDN SYVTPTQSVP
310 320 330 340
EYYDYGHGVN EDAYDSYAPE EWTTTRSSLK APPPRSARGG YREHPYGRY
Length:349
Mass (Da):38,854
Last modified:December 1, 2001 - v1
Checksum:iB8566F0211C1D942
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305618 mRNA. Translation: AAL09360.1.
RefSeqiNP_579852.1. NM_133318.1.
XP_008765174.1. XM_008766952.1.
UniGeneiRn.154423.

Genome annotation databases

EnsembliENSRNOT00000016578; ENSRNOP00000016579; ENSRNOG00000012284.
GeneIDi170843.
KEGGirno:170843.
UCSCiRGD:621738. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305618 mRNA. Translation: AAL09360.1.
RefSeqiNP_579852.1. NM_133318.1.
XP_008765174.1. XM_008766952.1.
UniGeneiRn.154423.

3D structure databases

ProteinModelPortaliQ920F3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016579.

PTM databases

iPTMnetiQ920F3.
PhosphoSiteiQ920F3.

Proteomic databases

PaxDbiQ920F3.
PRIDEiQ920F3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016578; ENSRNOP00000016579; ENSRNOG00000012284.
GeneIDi170843.
KEGGirno:170843.
UCSCiRGD:621738. rat.

Organism-specific databases

CTDi202559.
RGDi621738. Khdrbs2.

Phylogenomic databases

eggNOGiKOG1588. Eukaryota.
COG5176. LUCA.
GeneTreeiENSGT00550000074434.
HOGENOMiHOG000230771.
HOVERGENiHBG079164.
InParanoidiQ920F3.
KOiK17843.
OMAiPPIARGV.
OrthoDBiEOG75MVX3.
PhylomeDBiQ920F3.
TreeFamiTF314878.

Enzyme and pathway databases

ReactomeiR-RNO-8849468. PTK6 Regulates Proteins Involved in RNA Processing.

Miscellaneous databases

PROiQ920F3.

Gene expression databases

GenevisibleiQ920F3. RN.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR032571. Qua1_dom.
IPR032335. Sam68-YY.
[Graphical view]
PfamiPF00013. KH_1. 1 hit.
PF16274. Qua1. 1 hit.
PF16568. Sam68-YY. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "p59(fyn)-mediated phosphorylation regulates the activity of the tissue-specific splicing factor rSLM-1."
    Stoss O., Novoyatleva T., Gencheva M., Olbrich M., Benderska N., Stamm S.
    Mol. Cell. Neurosci. 27:8-21(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INTERACTION WITH RBMX; SAFB; SFRS9 AND YTHDC1, PHOSPHORYLATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA."
    Heinrich B., Zhang Z., Raitskin O., Hiller M., Benderska N., Hartmann A.M., Bracco L., Elliott D., Ben-Ari S., Soreq H., Sperling J., Sperling R., Stamm S.
    J. Biol. Chem. 284:14303-14315(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RBMX.

Entry informationi

Entry nameiKHDR2_RAT
AccessioniPrimary (citable) accession number: Q920F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.