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Protein

Proteinase-activated receptor 4

Gene

F2rl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

GO - Molecular functioni

  • G-protein coupled receptor activity Source: RGD
  • thrombin receptor activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 4
Short name:
PAR-4
Alternative name(s):
Coagulation factor II receptor-like 3
Thrombin receptor-like 3
Gene namesi
Name:F2rl3
Synonyms:Par4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620872. F2rl3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini59 – 9335ExtracellularSequence analysisAdd
BLAST
Transmembranei94 – 11421Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini115 – 1195CytoplasmicSequence analysis
Transmembranei120 – 14021Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini141 – 16121ExtracellularSequence analysisAdd
BLAST
Transmembranei162 – 18221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini183 – 20321CytoplasmicSequence analysisAdd
BLAST
Transmembranei204 – 22421Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini225 – 25430ExtracellularSequence analysisAdd
BLAST
Transmembranei255 – 27521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini276 – 29520CytoplasmicSequence analysisAdd
BLAST
Transmembranei296 – 31621Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini317 – 33014ExtracellularSequence analysisAdd
BLAST
Transmembranei331 – 35424Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini355 – 39541CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Propeptidei17 – 5842Removed for receptor activationBy similarityPRO_0000012766Add
BLAST
Chaini59 – 395337Proteinase-activated receptor 4PRO_0000012767Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence analysis
Disulfide bondi160 ↔ 239PROSITE-ProRule annotation

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei58 – 592Cleavage; by thrombinBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ920E0.
PRIDEiQ920E0.

PTM databases

PhosphoSiteiQ920E0.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065190.

Structurei

3D structure databases

ProteinModelPortaliQ920E0.
SMRiQ920E0. Positions 50-75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi98 – 1014Poly-Val
Compositional biasi373 – 3764Poly-Ser

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFSN. Eukaryota.
ENOG410YD35. LUCA.
HOVERGENiHBG096365.
InParanoidiQ920E0.
KOiK04236.
PhylomeDBiQ920E0.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003944. Prot_act_rcpt_4.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01430. PROTEASEAR4.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q920E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCWPLLYPLM LGFSISPAEC QTPSIYDDVE STREGQEASL RPTVELNESK
60 70 80 90 100
SPDKPNPRGF PGKPCANNSD TLELPASSEA LLLGWVPTRL VPAIYGLVVV
110 120 130 140 150
VGLPANGLAL WVLATRVPRL PSTILLMNLA VADLLLALVL PPRLVYHLRG
160 170 180 190 200
QRWPFGEAAC RVATAALYGH MYGSVLLLAA VSLDRYLALV HSLRARALRG
210 220 230 240 250
QRLTTILCLV AWLSAATLVL PLTFHRQTFL LAGSDRMLCH DALPLAEQTS
260 270 280 290 300
HWRPAFICLA VLGCFVPLLA MVLCYGATLR ALAANGQRYS HAVRLTALVL
310 320 330 340 350
FSAVAAFTPS NVLLVLHYSN PSPEAWGNLY GAYVPSLALS TLNSCVDPFI
360 370 380 390
YYYVSHEFRE KVRAMLCRQL KASSSSQASR EAGSRGTAIC SSTLL
Length:395
Mass (Da):42,943
Last modified:December 1, 2001 - v1
Checksum:i43B36D0DA22FAFAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 99Missing in AAK58604 (Ref. 2) Curated
Sequence conflicti192 – 1921S → P in AAK58604 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF310216 mRNA. Translation: AAL26790.1.
AF269246 mRNA. Translation: AAK58604.2.
RefSeqiNP_446260.1. NM_053808.1.
UniGeneiRn.81079.

Genome annotation databases

GeneIDi116498.
KEGGirno:116498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF310216 mRNA. Translation: AAL26790.1.
AF269246 mRNA. Translation: AAK58604.2.
RefSeqiNP_446260.1. NM_053808.1.
UniGeneiRn.81079.

3D structure databases

ProteinModelPortaliQ920E0.
SMRiQ920E0. Positions 50-75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065190.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ920E0.

Proteomic databases

PaxDbiQ920E0.
PRIDEiQ920E0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116498.
KEGGirno:116498.

Organism-specific databases

CTDi9002.
RGDi620872. F2rl3.

Phylogenomic databases

eggNOGiENOG410IFSN. Eukaryota.
ENOG410YD35. LUCA.
HOVERGENiHBG096365.
InParanoidiQ920E0.
KOiK04236.
PhylomeDBiQ920E0.

Miscellaneous databases

PROiQ920E0.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003944. Prot_act_rcpt_4.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01430. PROTEASEAR4.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Proteinase activated receptor 4 from rat duodenal library."
    Hoogerwerf W.A., Lee-Hellmich H., Pasricha P.J.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Duodenum.
  2. "Cloning of the rat protease activated receptor isoforms 3 and 4."
    Chien E.K., Marietti S., Mendoza J., Phillippe M.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Myometrium.

Entry informationi

Entry nameiPAR4_RAT
AccessioniPrimary (citable) accession number: Q920E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.