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Q920D2

- DYR_RAT

UniProt

Q920D2 - DYR_RAT

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Protein
Dihydrofolate reductase
Gene
Dhfr
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1 By similarity.

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei71 – 711Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 227NADP By similarity
Nucleotide bindingi55 – 573NADP By similarity
Nucleotide bindingi77 – 793NADP By similarity
Nucleotide bindingi117 – 1248NADP By similarity

GO - Molecular functioni

  1. NADP binding Source: RGD
  2. dihydrofolate reductase activity Source: UniProtKB
  3. dihydrofolic acid binding Source: RGD
  4. drug binding Source: UniProtKB
  5. mRNA binding Source: UniProtKB

GO - Biological processi

  1. dihydrofolate metabolic process Source: RGD
  2. glycine biosynthetic process Source: InterPro
  3. nucleotide biosynthetic process Source: InterPro
  4. one-carbon metabolic process Source: UniProtKB-KW
  5. response to methotrexate Source: UniProtKB-KW
  6. response to nicotine Source: RGD
  7. tetrahydrofolate biosynthetic process Source: RGD
  8. tetrahydrofolate metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Methotrexate resistance, One-carbon metabolism

Keywords - Ligandi

NADP, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_196414. G1/S-Specific Transcription.
REACT_199128. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
SABIO-RKQ920D2.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:Dhfr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi2500. Dhfr.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 187186Dihydrofolate reductase
PRO_0000186366Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331N6-acetyllysine; alternate By similarity
Modified residuei33 – 331N6-succinyllysine; alternate By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ920D2.
PRIDEiQ920D2.

PTM databases

PhosphoSiteiQ920D2.

Expressioni

Gene expression databases

GenevestigatoriQ920D2.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

MINTiMINT-4580348.

Structurei

3D structure databases

ProteinModelPortaliQ920D2.
SMRiQ920D2. Positions 2-187.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 185182DHFR
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 366Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0262.
GeneTreeiENSGT00390000010283.
HOGENOMiHOG000040235.
HOVERGENiHBG000773.
InParanoidiQ920D2.
KOiK00287.
OMAiLPWHPIR.
OrthoDBiEOG7V1FS3.
PhylomeDBiQ920D2.
TreeFamiTF317636.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q920D2-1 [UniParc]FASTAAdd to Basket

« Hide

MVRPLNCIVA VSQNMGIGKN GDLPWPLLRN EFKYFQRMTT TSSVEGKQNL    50
VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPQGAHFL AKSLDDALKL 100
IEQPELASKV DMVWVVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP 150
EIDLEKYKLL PEYPGVLSEI QEEKGIKYKF EVYEKKD 187
Length:187
Mass (Da):21,638
Last modified:January 23, 2007 - v3
Checksum:iFE1DB4F3515F8B26
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF318150 mRNA. Translation: AAL11500.1.
RefSeqiNP_569084.1. NM_130400.2.
UniGeneiRn.233753.
Rn.24681.

Genome annotation databases

EnsembliENSRNOT00000018259; ENSRNOP00000018259; ENSRNOG00000013521.
GeneIDi24312.
KEGGirno:24312.
UCSCiRGD:2500. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF318150 mRNA. Translation: AAL11500.1 .
RefSeqi NP_569084.1. NM_130400.2.
UniGenei Rn.233753.
Rn.24681.

3D structure databases

ProteinModelPortali Q920D2.
SMRi Q920D2. Positions 2-187.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4580348.

Chemistry

BindingDBi Q920D2.
ChEMBLi CHEMBL2097173.

PTM databases

PhosphoSitei Q920D2.

Proteomic databases

PaxDbi Q920D2.
PRIDEi Q920D2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000018259 ; ENSRNOP00000018259 ; ENSRNOG00000013521 .
GeneIDi 24312.
KEGGi rno:24312.
UCSCi RGD:2500. rat.

Organism-specific databases

CTDi 1719.
RGDi 2500. Dhfr.

Phylogenomic databases

eggNOGi COG0262.
GeneTreei ENSGT00390000010283.
HOGENOMi HOG000040235.
HOVERGENi HBG000773.
InParanoidi Q920D2.
KOi K00287.
OMAi LPWHPIR.
OrthoDBi EOG7V1FS3.
PhylomeDBi Q920D2.
TreeFami TF317636.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
Reactomei REACT_196414. G1/S-Specific Transcription.
REACT_199128. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
SABIO-RK Q920D2.

Miscellaneous databases

NextBioi 602953.
PROi Q920D2.

Gene expression databases

Genevestigatori Q920D2.

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme."
    Wang Y., Bruenn J.A., Queener S.F., Cody V.
    Antimicrob. Agents Chemother. 45:2517-2523(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiDYR_RAT
AccessioniPrimary (citable) accession number: Q920D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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