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Q920D2 (DYR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:Dhfr
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1 By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 187186Dihydrofolate reductase
PRO_0000186366

Regions

Domain4 – 185182DHFR
Nucleotide binding16 – 227NADP By similarity
Nucleotide binding55 – 573NADP By similarity
Nucleotide binding77 – 793NADP By similarity
Nucleotide binding117 – 1248NADP By similarity
Region31 – 366Substrate binding By similarity

Sites

Binding site101NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site711Substrate By similarity

Amino acid modifications

Modified residue331N6-acetyllysine; alternate By similarity
Modified residue331N6-succinyllysine; alternate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q920D2 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: FE1DB4F3515F8B26

FASTA18721,638
        10         20         30         40         50         60 
MVRPLNCIVA VSQNMGIGKN GDLPWPLLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS 

        70         80         90        100        110        120 
IPEKNRPLKD RINIVLSREL KEPPQGAHFL AKSLDDALKL IEQPELASKV DMVWVVGGSS 

       130        140        150        160        170        180 
VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLEKYKLL PEYPGVLSEI QEEKGIKYKF 


EVYEKKD 

« Hide

References

[1]"Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme."
Wang Y., Bruenn J.A., Queener S.F., Cody V.
Antimicrob. Agents Chemother. 45:2517-2523(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF318150 mRNA. Translation: AAL11500.1.
RefSeqNP_569084.1. NM_130400.2.
UniGeneRn.233753.
Rn.24681.

3D structure databases

ProteinModelPortalQ920D2.
SMRQ920D2. Positions 2-187.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4580348.

Chemistry

BindingDBQ920D2.
ChEMBLCHEMBL2097173.

PTM databases

PhosphoSiteQ920D2.

Proteomic databases

PaxDbQ920D2.
PRIDEQ920D2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018259; ENSRNOP00000018259; ENSRNOG00000013521.
GeneID24312.
KEGGrno:24312.
UCSCRGD:2500. rat.

Organism-specific databases

CTD1719.
RGD2500. Dhfr.

Phylogenomic databases

eggNOGCOG0262.
GeneTreeENSGT00390000010283.
HOGENOMHOG000040235.
HOVERGENHBG000773.
InParanoidQ920D2.
KOK00287.
OMALPWHPIR.
OrthoDBEOG7V1FS3.
PhylomeDBQ920D2.
TreeFamTF317636.

Enzyme and pathway databases

SABIO-RKQ920D2.
UniPathwayUPA00077; UER00158.

Gene expression databases

GenevestigatorQ920D2.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602953.
PROQ920D2.

Entry information

Entry nameDYR_RAT
AccessionPrimary (citable) accession number: Q920D2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways