Reviewed,
UniProtKB/Swiss-Prot Q920D2 (DYR_RAT)
Last modified
February 9, 2010.
Version 58.
History...
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50% identity |
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Dihydrofolate reductase EC=1.5.1.3 | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 187 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity. |
| Catalytic activity | 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Sequence similarities | Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 187 | 186 | Dihydrofolate reductase | PRO_0000186366 | |||||
Regions | |||||||||
| Domain | 4 – 185 | 182 | DHFR | ||||||
| Nucleotide binding | 16 – 22 | 7 | NADP By similarity | ||||||
| Nucleotide binding | 55 – 57 | 3 | NADP By similarity | ||||||
| Nucleotide binding | 77 – 79 | 3 | NADP By similarity | ||||||
| Nucleotide binding | 117 – 124 | 8 | NADP By similarity | ||||||
| Region | 31 – 36 | 6 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Binding site | 10 | 1 | NADP; via amide nitrogen and carbonyl oxygen By similarity | ||||||
| Binding site | 71 | 1 | Substrate By similarity | ||||||
Sequences
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References
| [1] | "Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme." Wang Y., Bruenn J.A., Queener S.F., Cody V. Antimicrob. Agents Chemother. 45:2517-2523(2001) [PubMed: 11502523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF318150 mRNA. Translation: AAL11500.1. |
| IPI | IPI00200419. |
| RefSeq | NP_569084.1. |
| UniGene | Rn.24681 |
3D structure databases | |
| SMR | Q920D2. Positions 2-187. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q920D2. |
PTM databases | |
| PhosphoSite | Q920D2. |
Proteomic databases | |
| PRIDE | Q920D2. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000018259; ENSRNOP00000018259; ENSRNOG00000013521; Rattus norvegicus. [Genome view] |
| GeneID | 24312. |
| KEGG | rno:24312. |
| NMPDR | fig|10116.3.peg.15341. |
| UCSC | NM_130400. rat. |
Organism-specific databases | |
| CTD | 24312. |
| RGD | 2500. Dhfr. |
Phylogenomic databases | |
| eggNOG | maNOG18401. |
| HOVERGEN | Q920D2. |
| InParanoid | Q920D2. |
| OMA | PLNCIVA. |
| OrthoDB | EOG97M4J0. |
| PhylomeDB | Q920D2. |
Enzyme and pathway databases | |
| BRENDA | 1.5.1.3. 248. |
Gene expression databases | |
| ArrayExpress | Q920D2. |
| Genevestigator | Q920D2. |
Family and domain databases | |
| InterPro | IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view] |
| Pfam | PF00186. DHFR_1. 1 hit. [Graphical view] |
| PRINTS | PR00070. DHFR. |
| PROSITE | PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| BindingDB | Q920D2. |
| NextBio | 602953. |
Entry information
| Entry name | DYR_RAT | ||||||||
| Accession | Primary (citable) accession number: Q920D2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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