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Reviewed, UniProtKB/Swiss-Prot Q920D2 (DYR_RAT)

Last modified November 3, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Dihydrofolate reductase
    EC=1.5.1.3
Gene names
Name: Dhfr
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Miscellaneous

The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 187186Dihydrofolate reductase
PRO_0000186366

Regions

Domain4 – 185182DHFR

Sequences

Sequence LengthMass (Da)Tools
Q920D2-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: FE1DB4F3515F8B26

FASTA18721,638
        10         20         30         40         50         60 
MVRPLNCIVA VSQNMGIGKN GDLPWPLLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS 

        70         80         90        100        110        120 
IPEKNRPLKD RINIVLSREL KEPPQGAHFL AKSLDDALKL IEQPELASKV DMVWVVGGSS 

       130        140        150        160        170        180 
VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLEKYKLL PEYPGVLSEI QEEKGIKYKF 


EVYEKKD 

« Hide

References

[1]"Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme."
Wang Y., Bruenn J.A., Queener S.F., Cody V.
Antimicrob. Agents Chemother. 45:2517-2523(2001) [PubMed: 11502523] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF318150 mRNA. Translation: AAL11500.1.
IPIIPI00200419.
RefSeqNP_569084.1.
UniGeneRn.24681

3D structure databases

HSSPHSSP built from PDB template 1KMS based on UniProtKB P00374.
SMRQ920D2. Positions 1-186, 2-187.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ920D2.

PTM databases

PhosphoSiteQ920D2.

Proteomic databases

PRIDEQ920D2.

Genome annotation databases

EnsemblENSRNOT00000018259; ENSRNOP00000018259; ENSRNOG00000013521; Rattus norvegicus. [Genome view]
GeneID24312.
KEGGrno:24312.
NMPDRfig|10116.3.peg.15341.
UCSCNM_130400. rat.

Organism-specific databases

CTD24312.
RGD2500. Dhfr.

Phylogenomic databases

HOVERGENQ920D2.
OMAPLNCIVA.

Enzyme and pathway databases

BRENDA1.5.1.3. 248.

Gene expression databases

ArrayExpressQ920D2.
GenevestigatorQ920D2.

Family and domain databases

InterProIPR012259. DHFR.
IPR001796. DHFR_reg.
IPR017925. Dihydrofolate_reductase_CS.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

BindingDBQ920D2.
NextBio602953.

Entry information

Entry nameDYR_RAT
AccessionPrimary (citable) accession number: Q920D2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 54 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents