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Q920D2

- DYR_RAT

UniProt

Q920D2 - DYR_RAT

Protein

Dihydrofolate reductase

Gene

Dhfr

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1 By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101NADP; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei71 – 711SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 227NADPBy similarity
    Nucleotide bindingi55 – 573NADPBy similarity
    Nucleotide bindingi77 – 793NADPBy similarity
    Nucleotide bindingi117 – 1248NADPBy similarity

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB
    2. dihydrofolic acid binding Source: RGD
    3. drug binding Source: UniProtKB
    4. mRNA binding Source: UniProtKB
    5. NADP binding Source: RGD

    GO - Biological processi

    1. dihydrofolate metabolic process Source: RGD
    2. glycine biosynthetic process Source: InterPro
    3. nucleotide biosynthetic process Source: InterPro
    4. one-carbon metabolic process Source: UniProtKB-KW
    5. response to methotrexate Source: UniProtKB-KW
    6. response to nicotine Source: RGD
    7. tetrahydrofolate biosynthetic process Source: RGD
    8. tetrahydrofolate metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Methotrexate resistance, One-carbon metabolism

    Keywords - Ligandi

    NADP, RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_196414. G1/S-Specific Transcription.
    REACT_199128. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    SABIO-RKQ920D2.
    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Gene namesi
    Name:Dhfr
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi2500. Dhfr.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 187186Dihydrofolate reductasePRO_0000186366Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei33 – 331N6-acetyllysine; alternateBy similarity
    Modified residuei33 – 331N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ920D2.
    PRIDEiQ920D2.

    PTM databases

    PhosphoSiteiQ920D2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ920D2.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    MINTiMINT-4580348.

    Structurei

    3D structure databases

    ProteinModelPortaliQ920D2.
    SMRiQ920D2. Positions 2-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 185182DHFRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni31 – 366Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    GeneTreeiENSGT00390000010283.
    HOGENOMiHOG000040235.
    HOVERGENiHBG000773.
    InParanoidiQ920D2.
    KOiK00287.
    OMAiLPWHPIR.
    OrthoDBiEOG7V1FS3.
    PhylomeDBiQ920D2.
    TreeFamiTF317636.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q920D2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRPLNCIVA VSQNMGIGKN GDLPWPLLRN EFKYFQRMTT TSSVEGKQNL    50
    VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPQGAHFL AKSLDDALKL 100
    IEQPELASKV DMVWVVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP 150
    EIDLEKYKLL PEYPGVLSEI QEEKGIKYKF EVYEKKD 187
    Length:187
    Mass (Da):21,638
    Last modified:January 23, 2007 - v3
    Checksum:iFE1DB4F3515F8B26
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF318150 mRNA. Translation: AAL11500.1.
    RefSeqiNP_569084.1. NM_130400.2.
    UniGeneiRn.233753.
    Rn.24681.

    Genome annotation databases

    EnsembliENSRNOT00000018259; ENSRNOP00000018259; ENSRNOG00000013521.
    GeneIDi24312.
    KEGGirno:24312.
    UCSCiRGD:2500. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF318150 mRNA. Translation: AAL11500.1 .
    RefSeqi NP_569084.1. NM_130400.2.
    UniGenei Rn.233753.
    Rn.24681.

    3D structure databases

    ProteinModelPortali Q920D2.
    SMRi Q920D2. Positions 2-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4580348.

    Chemistry

    BindingDBi Q920D2.
    ChEMBLi CHEMBL2097173.

    PTM databases

    PhosphoSitei Q920D2.

    Proteomic databases

    PaxDbi Q920D2.
    PRIDEi Q920D2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018259 ; ENSRNOP00000018259 ; ENSRNOG00000013521 .
    GeneIDi 24312.
    KEGGi rno:24312.
    UCSCi RGD:2500. rat.

    Organism-specific databases

    CTDi 1719.
    RGDi 2500. Dhfr.

    Phylogenomic databases

    eggNOGi COG0262.
    GeneTreei ENSGT00390000010283.
    HOGENOMi HOG000040235.
    HOVERGENi HBG000773.
    InParanoidi Q920D2.
    KOi K00287.
    OMAi LPWHPIR.
    OrthoDBi EOG7V1FS3.
    PhylomeDBi Q920D2.
    TreeFami TF317636.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    Reactomei REACT_196414. G1/S-Specific Transcription.
    REACT_199128. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
    SABIO-RK Q920D2.

    Miscellaneous databases

    NextBioi 602953.
    PROi Q920D2.

    Gene expression databases

    Genevestigatori Q920D2.

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme."
      Wang Y., Bruenn J.A., Queener S.F., Cody V.
      Antimicrob. Agents Chemother. 45:2517-2523(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.

    Entry informationi

    Entry nameiDYR_RAT
    AccessioniPrimary (citable) accession number: Q920D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3