Reviewed, UniProtKB/Swiss-Prot Q920D2 (DYR_RAT)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:

Dhfr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 187	186	Dihydrofolate reductase		PRO_0000186366
Regions
	Domain	4 – 185	182	DHFR

Sequences

	Sequence		Length	Mass (Da)
	Q920D2-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FE1DB4F3515F8B26	FASTA	187	21,638
	10 20 30 40 50 60 MVRPLNCIVA VSQNMGIGKN GDLPWPLLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS 70 80 90 100 110 120 IPEKNRPLKD RINIVLSREL KEPPQGAHFL AKSLDDALKL IEQPELASKV DMVWVVGGSS 130 140 150 160 170 180 VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLEKYKLL PEYPGVLSEI QEEKGIKYKF EVYEKKD

References

[1]	"Isolation of rat dihydrofolate reductase gene and characterization of recombinant enzyme." Wang Y., Bruenn J.A., Queener S.F., Cody V. Antimicrob. Agents Chemother. 45:2517-2523(2001) [PubMed: 11502523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF318150 mRNA. Translation: AAL11500.1.
RefSeq	NP_569084.1.
UniGene	Rn.24681
3D structure databases
HSSP	HSSP built from PDB template 1KMS based on UniProtKB P00374.
SMR	Q920D2. Positions 1-186, 2-187.
ModBase	Search...
PTM databases
PhosphoSite	Q920D2.
Genome annotation databases
Ensembl	ENSRNOG00000013521. Rattus norvegicus. [Contig view]
GeneID	24312.
KEGG	rno:24312.
NMPDR	fig|10116.3.peg.15341.
Organism-specific databases
RGD	2500. Dhfr.
Phylogenomic databases
HOVERGEN	Q920D2.
Family and domain databases
InterPro	IPR001796. DHFR_reg. [Graphical view]
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	Q920D2.
NextBio	602953.

Entry information

Entry name

DYR_RAT

Accession

Primary (citable) accession number: Q920D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 43 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)