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Protein

Chordin-like protein 1

Gene

Chrdl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation (By similarity). Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development (By similarity).By similarity

GO - Biological processi

  1. compound eye development Source: UniProtKB
  2. eye development Source: UniProtKB
  3. negative regulation of BMP signaling pathway Source: MGI
  4. neuron differentiation Source: InterPro
  5. ossification Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Osteogenesis

Enzyme and pathway databases

ReactomeiREACT_334226. Signaling by BMP.

Names & Taxonomyi

Protein namesi
Recommended name:
Chordin-like protein 1
Alternative name(s):
Neuralin-1
Neurogenesin-1
Ventroptin
Gene namesi
Name:Chrdl1
Synonyms:Ng1, Nrln1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1933172. Chrdl1.

Subcellular locationi

  1. Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 447425Chordin-like protein 1PRO_0000005369Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

May be glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ920C1.

PTM databases

PhosphoSiteiQ920C1.

Expressioni

Tissue specificityi

Expressed in heart, brain, lung, liver, kidney and testis.

Developmental stagei

Expression starts in the neural plate at mid-gastrulation. Later on its expression becomes restricted to discrete regions of the central nervous system and to derivatives of the neural crest cells. Expressed as well in the primordial cells of the skeleton in mice embryos at 13.5 dpc.1 Publication

Gene expression databases

BgeeiQ920C1.
CleanExiMM_CHRDL1.
ExpressionAtlasiQ920C1. baseline and differential.
GenevestigatoriQ920C1.

Structurei

3D structure databases

ProteinModelPortaliQ920C1.
SMRiQ920C1. Positions 29-99, 105-175, 251-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 9566VWFC 1PROSITE-ProRule annotationAdd
BLAST
Domaini108 – 17467VWFC 2PROSITE-ProRule annotationAdd
BLAST
Domaini253 – 31866VWFC 3PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi174 – 1763Cell attachment siteSequence Analysis

Sequence similaritiesi

Contains 3 VWFC domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG147797.
GeneTreeiENSGT00730000110792.
HOGENOMiHOG000049211.
HOVERGENiHBG051113.
InParanoidiQ920C1.
OMAiCPRCPED.
OrthoDBiEOG7XH6S0.
PhylomeDBiQ920C1.

Family and domain databases

InterProiIPR030114. Chordin-like_1.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR11339:SF249. PTHR11339:SF249. 1 hit.
PfamiPF00093. VWC. 3 hits.
[Graphical view]
SMARTiSM00214. VWC. 3 hits.
[Graphical view]
PROSITEiPS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q920C1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGMKYIISL FFIFVFLEGS KTEQVKHSDT YCVFQDKKYR VGEKWHPYLE
60 70 80 90 100
PYGLVYCVNC ICSENGNVLC SRVRCPSLHC LSPVHIPHLC CPRCPDSLPP
110 120 130 140 150
VNNKVTSKSC EYNGTTYQHG ELFIAEGLFQ NRQPNQCSQC SCSEGNVYCG
160 170 180 190 200
LKTCPKLTCA FPVSVPDSCC RVCRGDAELS WEHADGDIFR QPANREARHS
210 220 230 240 250
YLRSPYDPPP NRQAGGLPRF PGSRSHRGAV IDSQQASGTI VQIVINNKHK
260 270 280 290 300
HGQVCVSNGK TYSHGESWHP NLRAFGIVEC VLCTCNVTKQ ECKKIHCPNR
310 320 330 340 350
YPCKYPQKID GKCCKVCPEE PPSQNFDSKG SFCGEETMPV YESVFMEDGE
360 370 380 390 400
TTRKVALETE RPPQVEVHVW TIQKGILQHF HIEKISKRMF GELHHFKLVT
410 420 430 440
RTTLNQWKLF TEGEAQLSQM CSSQVCRTEL EDLVQVLYLG RPEKDHC
Length:447
Mass (Da):50,732
Last modified:December 1, 2001 - v1
Checksum:i94C999025A97545D
GO
Isoform Beta (identifier: Q920C1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-333: EEPPSQNFDSKGSFC → GKKAKGALAGGPAFG
     334-447: Missing.

Show »
Length:333
Mass (Da):37,034
Checksum:iB9D08DE5AC74E1D6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti211 – 2111N → S in AAG27460 (PubMed:11118896).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei319 – 33315EEPPS…KGSFC → GKKAKGALAGGPAFG in isoform Beta. 2 PublicationsVSP_001076Add
BLAST
Alternative sequencei334 – 447114Missing in isoform Beta. 2 PublicationsVSP_001077Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321853 mRNA. Translation: AAK95586.1.
AF296451 mRNA. Translation: AAK71523.1.
AF305714 mRNA. Translation: AAG27460.1.
CCDSiCCDS30453.1. [Q920C1-2]
CCDS53208.1. [Q920C1-1]
RefSeqiNP_001107857.1. NM_001114385.1. [Q920C1-1]
NP_112548.2. NM_031258.3. [Q920C1-2]
UniGeneiMm.157697.

Genome annotation databases

EnsembliENSMUST00000063029; ENSMUSP00000056193; ENSMUSG00000031283. [Q920C1-1]
ENSMUST00000074660; ENSMUSP00000074230; ENSMUSG00000031283. [Q920C1-2]
ENSMUST00000112878; ENSMUSP00000108499; ENSMUSG00000031283. [Q920C1-1]
ENSMUST00000166406; ENSMUSP00000130284; ENSMUSG00000031283. [Q920C1-2]
GeneIDi83453.
KEGGimmu:83453.
UCSCiuc009ume.2. mouse. [Q920C1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321853 mRNA. Translation: AAK95586.1.
AF296451 mRNA. Translation: AAK71523.1.
AF305714 mRNA. Translation: AAG27460.1.
CCDSiCCDS30453.1. [Q920C1-2]
CCDS53208.1. [Q920C1-1]
RefSeqiNP_001107857.1. NM_001114385.1. [Q920C1-1]
NP_112548.2. NM_031258.3. [Q920C1-2]
UniGeneiMm.157697.

3D structure databases

ProteinModelPortaliQ920C1.
SMRiQ920C1. Positions 29-99, 105-175, 251-323.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ920C1.

Proteomic databases

PRIDEiQ920C1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063029; ENSMUSP00000056193; ENSMUSG00000031283. [Q920C1-1]
ENSMUST00000074660; ENSMUSP00000074230; ENSMUSG00000031283. [Q920C1-2]
ENSMUST00000112878; ENSMUSP00000108499; ENSMUSG00000031283. [Q920C1-1]
ENSMUST00000166406; ENSMUSP00000130284; ENSMUSG00000031283. [Q920C1-2]
GeneIDi83453.
KEGGimmu:83453.
UCSCiuc009ume.2. mouse. [Q920C1-1]

Organism-specific databases

CTDi91851.
MGIiMGI:1933172. Chrdl1.

Phylogenomic databases

eggNOGiNOG147797.
GeneTreeiENSGT00730000110792.
HOGENOMiHOG000049211.
HOVERGENiHBG051113.
InParanoidiQ920C1.
OMAiCPRCPED.
OrthoDBiEOG7XH6S0.
PhylomeDBiQ920C1.

Enzyme and pathway databases

ReactomeiREACT_334226. Signaling by BMP.

Miscellaneous databases

NextBioi350576.
PROiQ920C1.
SOURCEiSearch...

Gene expression databases

BgeeiQ920C1.
CleanExiMM_CHRDL1.
ExpressionAtlasiQ920C1. baseline and differential.
GenevestigatoriQ920C1.

Family and domain databases

InterProiIPR030114. Chordin-like_1.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR11339:SF249. PTHR11339:SF249. 1 hit.
PfamiPF00093. VWC. 3 hits.
[Graphical view]
SMARTiSM00214. VWC. 3 hits.
[Graphical view]
PROSITEiPS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ventroptin: a BMP-4 antagonist expressed in a double-gradient pattern in the retina."
    Sakuta H., Suzuki R., Takahashi H., Kato A., Shintani T., Iemura S., Yamamoto T.S., Ueno N., Noda M.
    Science 293:111-115(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
    Strain: C57BL/6J.
  2. "Neuralin-1 is a novel chordin-related molecule expressed in the mouse neural plate."
    Coffinier C.C., Tran U., Larrain J., De Robertis E.M.
    Mech. Dev. 100:119-122(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
  3. "Neurogenesin-1 differentially inhibits the osteoblastic differentiation by bone morphogenetic proteins in C2C12 cells."
    Chandra A., Itakura T., Yang Z., Tamakoshi T., Xue X., Wang B., Ueki T., Sato K., Uezato T., Miura N.
    Biochem. Biophys. Res. Commun. 344:786-791(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GLYCOSYLATION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCRDL1_MOUSE
AccessioniPrimary (citable) accession number: Q920C1
Secondary accession number(s): Q924K0, Q9EPZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.