Q920C1 (CRDL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chordin-like protein 1

Alternative name(s):
Neuralin-1
Neurogenesin-1
Ventroptin

Gene names

Name:	Chrdl1
Synonyms:	Ng1, Nrln1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation By similarity. Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development By similarity. Ref.3
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in heart, brain, lung, liver, kidney and testis.
Developmental stage	Expression starts in the neural plate at mid-gastrulation. Later on its expression becomes restricted to discrete regions of the central nervous system and to derivatives of the neural crest cells. Expressed as well in the primordial cells of the skeleton in mice embryos at 13.5 dpc. Ref.3
Post-translational modification	May be glycosylated. Ref.3
Sequence similarities	Contains 3 VWFC domains.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Osteogenesis
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW compound eye development Inferred from sequence or structural similarity. Source: UniProtKB nervous system development Inferred from electronic annotation. Source: UniProtKB-KW ossification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha (identifier: Q920C1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: Q920C1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     319-333: EEPPSQNFDSKGSFC → GKKAKGALAGGPAFG
     334-447: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 447	425	Chordin-like protein 1		PRO_0000005369
Regions
	Domain	30 – 95	66	VWFC 1
	Domain	108 – 174	67	VWFC 2
	Domain	253 – 318	66	VWFC 3
	Motif	174 – 176	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	286	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	319 – 333	15	EEPPS…KGSFC → GKKAKGALAGGPAFG in isoform Beta.		VSP_001076
	Alternative sequence	334 – 447	114	Missing in isoform Beta.		VSP_001077
Experimental info
	Sequence conflict	211	1	N → S in AAG27460. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 94C999025A97545D	FASTA	447	50,732
	10 20 30 40 50 60 MDGMKYIISL FFIFVFLEGS KTEQVKHSDT YCVFQDKKYR VGEKWHPYLE PYGLVYCVNC 70 80 90 100 110 120 ICSENGNVLC SRVRCPSLHC LSPVHIPHLC CPRCPDSLPP VNNKVTSKSC EYNGTTYQHG 130 140 150 160 170 180 ELFIAEGLFQ NRQPNQCSQC SCSEGNVYCG LKTCPKLTCA FPVSVPDSCC RVCRGDAELS 190 200 210 220 230 240 WEHADGDIFR QPANREARHS YLRSPYDPPP NRQAGGLPRF PGSRSHRGAV IDSQQASGTI 250 260 270 280 290 300 VQIVINNKHK HGQVCVSNGK TYSHGESWHP NLRAFGIVEC VLCTCNVTKQ ECKKIHCPNR 310 320 330 340 350 360 YPCKYPQKID GKCCKVCPEE PPSQNFDSKG SFCGEETMPV YESVFMEDGE TTRKVALETE 370 380 390 400 410 420 RPPQVEVHVW TIQKGILQHF HIEKISKRMF GELHHFKLVT RTTLNQWKLF TEGEAQLSQM 430 440 CSSQVCRTEL EDLVQVLYLG RPEKDHC
	Isoform Beta [UniParc]. Checksum: B9D08DE5AC74E1D6	FASTA	333	37,034
	10 20 30 40 50 60 MDGMKYIISL FFIFVFLEGS KTEQVKHSDT YCVFQDKKYR VGEKWHPYLE PYGLVYCVNC 70 80 90 100 110 120 ICSENGNVLC SRVRCPSLHC LSPVHIPHLC CPRCPDSLPP VNNKVTSKSC EYNGTTYQHG 130 140 150 160 170 180 ELFIAEGLFQ NRQPNQCSQC SCSEGNVYCG LKTCPKLTCA FPVSVPDSCC RVCRGDAELS 190 200 210 220 230 240 WEHADGDIFR QPANREARHS YLRSPYDPPP NRQAGGLPRF PGSRSHRGAV IDSQQASGTI 250 260 270 280 290 300 VQIVINNKHK HGQVCVSNGK TYSHGESWHP NLRAFGIVEC VLCTCNVTKQ ECKKIHCPNR 310 320 330 YPCKYPQKID GKCCKVCPGK KAKGALAGGP AFG

References

[1]	"Ventroptin: a BMP-4 antagonist expressed in a double-gradient pattern in the retina." Sakuta H., Suzuki R., Takahashi H., Kato A., Shintani T., Iemura S., Yamamoto T.S., Ueno N., Noda M. Science 293:111-115(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). Strain: C57BL/6J.
[2]	"Neuralin-1 is a novel chordin-related molecule expressed in the mouse neural plate." Coffinier C.C., Tran U., Larrain J., De Robertis E.M. Mech. Dev. 100:119-122(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
[3]	"Neurogenesin-1 differentially inhibits the osteoblastic differentiation by bone morphogenetic proteins in C2C12 cells." Chandra A., Itakura T., Yang Z., Tamakoshi T., Xue X., Wang B., Ueki T., Sato K., Uezato T., Miura N. Biochem. Biophys. Res. Commun. 344:786-791(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, GLYCOSYLATION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF321853 mRNA. Translation: AAK95586.1. AF296451 mRNA. Translation: AAK71523.1. AF305714 mRNA. Translation: AAG27460.1.
IPI	IPI00230343. IPI00284757.
RefSeq	NP_001107857.1. NM_001114385.1. NP_112548.2. NM_031258.3.
UniGene	Mm.157697.
3D structure databases
ProteinModelPortal	Q920C1.
SMR	Q920C1. Positions 29-99, 105-175, 251-324.
ModBase	Search...
PTM databases
PhosphoSite	Q920C1.
Proteomic databases
PRIDE	Q920C1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000063029; ENSMUSP00000056193; ENSMUSG00000031283. ENSMUST00000074660; ENSMUSP00000074230; ENSMUSG00000031283. ENSMUST00000112878; ENSMUSP00000108499; ENSMUSG00000031283. ENSMUST00000166406; ENSMUSP00000130284; ENSMUSG00000031283.
GeneID	83453.
KEGG	mmu:83453.
Organism-specific databases
CTD	91851.
MGI	MGI:1933172. Chrdl1.
Phylogenomic databases
eggNOG	NOG147797.
GeneTree	ENSGT00700000104447.
HOGENOM	HOG000049211.
HOVERGEN	HBG051113.
InParanoid	Q920C1.
OrthoDB	EOG46Q6SD.
Gene expression databases
ArrayExpress	Q920C1.
Bgee	Q920C1.
CleanEx	MM_CHRDL1.
Genevestigator	Q920C1.
GermOnline	ENSMUSG00000031283. Mus musculus.
Family and domain databases
InterPro	IPR001007. VWF_C. [Graphical view]
Pfam	PF00093. VWC. 3 hits. [Graphical view]
SMART	SM00214. VWC. 3 hits. [Graphical view]
PROSITE	PS01208. VWFC_1. 3 hits. PS50184. VWFC_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	350576.
SOURCE	Search...

Entry information

Entry name

CRDL1_MOUSE

Accession

Primary (citable) accession number: Q920C1
Secondary accession number(s): Q924K0, Q9EPZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program