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Protein

Potassium channel subfamily K member 2

Gene

Kcnk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner (PubMed:11319556). In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (By similarity).By similarity1 Publication
Isoform 5: Does not display channel activity but reduces the channel activity of isoform 1, isoform 2 and isoform 4 and reduces cell surface expression of isoform 2.1 Publication

Miscellaneous

Activated by arachadonic acid, mechanical stretching and intracellular acidification.1 Publication

GO - Molecular functioni

  • outward rectifier potassium channel activity Source: UniProtKB
  • potassium channel inhibitor activity Source: UniProtKB
  • potassium ion leak channel activity Source: UniProtKB

GO - Biological processi

  • cardiac ventricle development Source: RGD
  • cellular response to hypoxia Source: RGD
  • cochlea development Source: RGD
  • G-protein coupled receptor signaling pathway Source: Ensembl
  • memory Source: RGD
  • negative regulation of cardiac muscle cell proliferation Source: RGD
  • negative regulation of DNA biosynthetic process Source: RGD
  • positive regulation of cell death Source: RGD
  • positive regulation of cellular response to hypoxia Source: RGD
  • potassium ion transmembrane transport Source: UniProtKB
  • response to axon injury Source: RGD
  • response to mechanical stimulus Source: RGD
  • stabilization of membrane potential Source: GO_Central

Keywordsi

Molecular functionIon channel
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-1299503 TWIK related potassium channel (TREK)
R-RNO-5576886 Phase 4 - resting membrane potential

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 2By similarity
Alternative name(s):
Outward rectifying potassium channel protein TREK-1By similarity
Stretch-activated potassium channel TREK-11 Publication
TREK-1 K(+) channel subunitBy similarity
Two pore domain potassium channel TREK-11 Publication
Two pore potassium channel TPKC1By similarity
Gene namesi
Name:Kcnk2Imported
Synonyms:TrekImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi621448 Kcnk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 61CytoplasmicSequence analysisAdd BLAST61
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Intramembranei144 – 170Pore-forming; Name=Pore-forming 1Sequence analysisAdd BLAST27
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Topological domaini193 – 222CytoplasmicSequence analysisAdd BLAST30
Transmembranei223 – 243HelicalSequence analysisAdd BLAST21
Intramembranei253 – 283Pore-forming; Name=Pore-forming 2Sequence analysisAdd BLAST31
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Topological domaini309 – 426CytoplasmicSequence analysisAdd BLAST118

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3817718

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004267181 – 426Potassium channel subfamily K member 2Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi108InterchainBy similarity
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei348Phosphoserine; by PKABy similarity1

Post-translational modificationi

Phosphorylation at Ser-348 controls the reversible conversion from a leak channel to a voltage-dependent channel.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ920B6
PRIDEiQ920B6

Expressioni

Tissue specificityi

Expressed in cardiomyocytes (at protein level). Expressed in various brain regions including the lateral olfactory tract, piriform cortex of the forebrain, paraventricular and anteromedial thalamic nuclei, brainstem, caudate putamen, nucleus accumbens, neocortex and interpeduncular nucleus. Isoform 5 is expressed in brain and kidney.3 Publications

Gene expression databases

BgeeiENSRNOG00000002653
GenevisibleiQ920B6 RN

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Heterodimer with KCNK1; disulfide-linked (By similarity). Interacts with BVES; the interaction enhances KCNK2 surface expression and is inhibited by cAMP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GNG4P501503EBI-6530063,EBI-6395970From Homo sapiens.

Protein-protein interaction databases

IntActiQ920B6, 9 interactors
STRINGi10116.ENSRNOP00000003684

Structurei

3D structure databases

ProteinModelPortaliQ920B6
SMRiQ920B6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni354 – 426Required for basal channel activityBy similarityAdd BLAST73
Regioni378 – 426Essential for chloroform and halothane sensitivityBy similarityAdd BLAST49

Domaini

The C-terminal region of isoform 5 mediates its intracellular retention.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1418 Eukaryota
COG1226 LUCA
GeneTreeiENSGT00760000118858
HOGENOMiHOG000013106
HOVERGENiHBG052234
InParanoidiQ920B6
KOiK04913
OMAiAINVMKW
OrthoDBiEOG091G0E3R
PhylomeDBiQ920B6
TreeFamiTF313947

Family and domain databases

InterProiView protein in InterPro
IPR003280 2pore_dom_K_chnl
IPR003976 2pore_dom_K_chnl_TREK
IPR013099 K_chnl_dom
PfamiView protein in Pfam
PF07885 Ion_trans_2, 2 hits
PRINTSiPR01333 2POREKCHANEL
PR01499 TREKCHANNEL

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: Q920B6-1) [UniParc]FASTAAdd to basket
Also known as: TREK-1b1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLASASRERP GYTAGVAAPD LLDPKSAAQN SKPRLSFSAK PTVLASRVES
60 70 80 90 100
DSAINVMKWK TVSTIFLVVV LYLIIGATVF KALEQPQEIS QRTTIVIQKQ
110 120 130 140 150
NFIAQHACVN STELDELIQQ IVTAINAGII PLGNNSNQVS HWDLGSSFFF
160 170 180 190 200
AGTVITTIGF GNISPRTEGG KIFCIIYALL GIPLFGFLLA GVGDQLGTIF
210 220 230 240 250
GKGIAKVEDT FIKWNVSQTK IRIISTIIFI LFGCVLFVAL PAVIFKHIEG
260 270 280 290 300
WSALDAIYFV VITLTTIGFG DYVAGGSDIE YLDFYKPVVW FWILVGLAYF
310 320 330 340 350
AAVLSMIGDW LRVISKKTKE EVGEFRAHAA EWTANVTAEF KETRRRLSVE
360 370 380 390 400
IYDKFQRATS VKRKLSAELA GNHNQELTPC RRTLSVNHLT SEREVLPPLL
410 420
KAESIYLNGL TPHCAAEDIA VIENMK
Length:426
Mass (Da):46,912
Last modified:December 1, 2001 - v1
Checksum:iCACDA05BBE95FDBC
GO
Isoform 21 Publication (identifier: Q920B6-2) [UniParc]FASTAAdd to basket
Also known as: TREK-1a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     2-16: Missing.

Show »
Length:411
Mass (Da):45,396
Checksum:iAEA49DEB352F1F93
GO
Isoform 31 Publication (identifier: Q920B6-3) [UniParc]FASTAAdd to basket
Also known as: TREK-1c1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MLASASRERPGYTAGV → MMNPRAKRSVYL

Show »
Length:422
Mass (Da):46,712
Checksum:iF93C93197D1D5EA2
GO
Isoform 41 Publication (identifier: Q920B6-4) [UniParc]FASTAAdd to basket
Also known as: TREK-1d1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MLASASRERPGYTAG → MGA

Show »
Length:414
Mass (Da):45,623
Checksum:i6111010B94D42D5E
GO
Isoform 51 Publication (identifier: Q920B6-5) [UniParc]FASTAAdd to basket
Also known as: TREK-1e1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     2-16: Missing.
     213-241: KWNVSQTKIRIISTIIFILFGCVLFVALP → VGRTLNIWTSTSSSCGSGSSLGWPTLRLF
     242-426: Missing.

Show »
Length:226
Mass (Da):24,225
Checksum:iA78944060F5D788E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti416A → R in ABD64605 (PubMed:24196565).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0539521 – 16MLASA…YTAGV → MMNPRAKRSVYL in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_0539531 – 15MLASA…GYTAG → MGA in isoform 4. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_0539542 – 16Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST15
Alternative sequenceiVSP_053955213 – 241KWNVS…FVALP → VGRTLNIWTSTSSSCGSGSS LGWPTLRLF in isoform 5. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_053956242 – 426Missing in isoform 5. 1 PublicationAdd BLAST185

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325671 mRNA Translation: AAL01159.1
AF385402 mRNA Translation: AAL95708.1
AY727922 mRNA Translation: AAU25945.1
AY555072 mRNA Translation: AAT64134.1
AY555073 mRNA Translation: AAT64135.1
DQ403851 mRNA Translation: ABD64605.1
AY695826 mRNA Translation: AAU06141.1
AABR06076806 Genomic DNA No translation available.
AABR06076807 Genomic DNA No translation available.
AABR06076808 Genomic DNA No translation available.
AABR06076809 Genomic DNA No translation available.
CH473985 Genomic DNA Translation: EDL94961.1
CH473985 Genomic DNA Translation: EDL94962.1
RefSeqiNP_742038.2, NM_172041.2
NP_742039.1, NM_172042.1 [Q920B6-1]
XP_006250495.1, XM_006250433.3 [Q920B6-4]
XP_006250496.1, XM_006250434.3 [Q920B6-2]
UniGeneiRn.21984

Genome annotation databases

EnsembliENSRNOT00000003684; ENSRNOP00000003684; ENSRNOG00000002653 [Q920B6-1]
ENSRNOT00000077282; ENSRNOP00000068842; ENSRNOG00000002653 [Q920B6-3]
GeneIDi170899
KEGGirno:170899
UCSCiRGD:621448 rat [Q920B6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKCNK2_RAT
AccessioniPrimary (citable) accession number: Q920B6
Secondary accession number(s): A3QR52
, Q3MMY3, Q5DNW4, Q5DNW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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