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Protein

FERM domain-containing protein 4B

Gene

Frmd4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of GRP1 signaling complexes that are acutely recruited to plasma membrane ruffles in response to insulin receptor signaling. May function as a scaffolding protein.

GO - Biological processi

  • establishment of epithelial cell polarity Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
FERM domain-containing protein 4B
Alternative name(s):
GRP1-binding protein GRSP1
Golgi-associated band 4.1-like protein
Short name:
GOBLIN
Gene namesi
Name:Frmd4b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2141794. Frmd4b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • extracellular space Source: MGI
  • ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035FERM domain-containing protein 4BPRO_0000219447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei372 – 3721PhosphoserineCombined sources
Modified residuei609 – 6091PhosphoserineBy similarity
Modified residuei698 – 6981PhosphoserineCombined sources
Modified residuei916 – 9161PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ920B0.
MaxQBiQ920B0.
PaxDbiQ920B0.
PRIDEiQ920B0.

PTM databases

iPTMnetiQ920B0.
PhosphoSiteiQ920B0.

Expressioni

Tissue specificityi

Isoform 1 is expressed in the brain. Isoform 2 is expressed in the lung (at protein level).1 Publication

Gene expression databases

BgeeiQ920B0.
CleanExiMM_FRMD4B.
ExpressionAtlasiQ920B0. baseline and differential.
GenevisibleiQ920B0. MM.

Interactioni

Subunit structurei

Interacts with GRP1.1 Publication

Protein-protein interaction databases

BioGridi231236. 2 interactions.
STRINGi10090.ENSMUSP00000108982.

Structurei

3D structure databases

ProteinModelPortaliQ920B0.
SMRiQ920B0. Positions 68-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 361303FERMPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili414 – 45138Sequence analysisAdd
BLAST
Coiled coili535 – 55925Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3529. Eukaryota.
ENOG410XQFP. LUCA.
GeneTreeiENSGT00830000128251.
HOGENOMiHOG000294161.
HOVERGENiHBG062800.
InParanoidiQ920B0.
OMAiNEYRIRC.
OrthoDBiEOG7HF1HH.
PhylomeDBiQ920B0.
TreeFamiTF328984.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR021774. DUF3338.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF11819. DUF3338. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
SMARTiSM00295. B41. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q920B0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVFMCGVE DLLFSGSRFV WNLTVSTLRR WYTERLRACH QVLRTWCGLR
60 70 80 90 100
DVYQMTEGRH CQVHLLDDRR LELLVQPKLL SRELLDLVAS HFNLKEKEYF
110 120 130 140 150
GITFIDDTGQ ENWLQLDHRV LEHDLPKKPG PTLLHFAVRF YIESISFLKD
160 170 180 190 200
KNTVELFFLN AKACVHKGQI EVDSETIFKL AALVLQESKG DYTSDENARK
210 220 230 240 250
DLKTLPVFPT KTLQEHPSLA YCEDRVIEHY LKIKGLTRGQ AVVQYMKIVE
260 270 280 290 300
ALPTYGVHYY AVKDKQGLPW WLGISYKGIG QYDLQDKVKP RKLFQWKQLE
310 320 330 340 350
NLYFREKKFA VEVHDPRRIS VSRRTFGQSG LFVQTWYANS SLIKSIWVMA
360 370 380 390 400
ISQHQFYLDR KQSKAKIPSA RSLDDIAMDL TETGTQRGSK LVTLEAKSQF
410 420 430 440 450
IMASNGSLIS SGSQDSEGME EQKREKILEL KKKEKLLQEK LLQKVEELKK
460 470 480 490 500
ICLREAELTG RMPKEYPLNI GEKPPQVRRR VGTTFKLDDN LLPTEEDPAL
510 520 530 540 550
QELESNFLIQ QKLVEAAKKL ASEPDLCKTV KKKRKQDYTD AVKRLQEIEN
560 570 580 590 600
SINEYRIRCG KKPSQKAAVV PPEDIIPSES SSLSDTTTYD DPNDSFTLAG
610 620 630 640 650
QRPSSVPHSP RILPPKSLGI ERIHFRKSSI NEQFMDTRHS REMLSTHSSP
660 670 680 690 700
YKTLERRPQG GRSMPTTPVL TRNAYSSSHL EPDSSSQHCR QRSGSLESQS
710 720 730 740 750
HLLSEMDSDK PFFTLSKSQR SSSTEILDDG SSYTSQSSSE YYCVTPAASP
760 770 780 790 800
YYTTQTLDTR ARGRRRSKKH SVSTSNSGSM PNLAQKDPLR NGVYSKGQDP
810 820 830 840 850
PPSGYYIAGY PPYAECDLYY SGGYVYENDT EGQYSVNPSY RSSAHYGYDR
860 870 880 890 900
QRDYSRSFHE DEVDRVPHNP YATLRLPRKA AVKSEHITKN IHKALVAEHL
910 920 930 940 950
RGWYQRASGQ KDQGHSPQTS FDSDRGSQRC LGFAGLQVPC SPSSRASSYS
960 970 980 990 1000
SVSSTNASGN WRTQLTIGLS EYENPVHSPY TSYYGSIYNP LSSPSRQYAE
1010 1020 1030
TTPLDGTDGS QLEDNLEGSE QRLFWHEDSK PGTLV
Length:1,035
Mass (Da):118,007
Last modified:April 5, 2011 - v2
Checksum:i5CA90A96E3F3368A
GO
Isoform 2 (identifier: Q920B0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Show »
Length:981
Mass (Da):111,566
Checksum:iE8332CB283CF829A
GO

Sequence cautioni

The sequence AAL26916.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621Q → R in BAB17031 (Ref. 2) Curated
Sequence conflicti224 – 2241D → G in BAE21079 (PubMed:16141072).Curated
Sequence conflicti802 – 8021P → S in BAB17031 (Ref. 2) Curated
Sequence conflicti917 – 9182PQ → TK in BAB17031 (Ref. 2) Curated
Sequence conflicti986 – 9861S → N in BAB17031 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5454Missing in isoform 2. 3 PublicationsVSP_040896Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327856 mRNA. Translation: AAL26916.1. Different initiation.
AF327857 mRNA. Translation: AAL26917.1.
AB042027 mRNA. Translation: BAB17031.1.
AK132280 mRNA. Translation: BAE21079.1.
BC052390 mRNA. Translation: AAH52390.1.
CCDSiCCDS39577.1. [Q920B0-2]
RefSeqiNP_660130.2. NM_145148.2. [Q920B0-2]
XP_006506067.1. XM_006506004.2. [Q920B0-1]
UniGeneiMm.27789.

Genome annotation databases

EnsembliENSMUST00000032146; ENSMUSP00000032146; ENSMUSG00000030064. [Q920B0-1]
ENSMUST00000113355; ENSMUSP00000108982; ENSMUSG00000030064. [Q920B0-2]
GeneIDi232288.
KEGGimmu:232288.
UCSCiuc009dat.1. mouse. [Q920B0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327856 mRNA. Translation: AAL26916.1. Different initiation.
AF327857 mRNA. Translation: AAL26917.1.
AB042027 mRNA. Translation: BAB17031.1.
AK132280 mRNA. Translation: BAE21079.1.
BC052390 mRNA. Translation: AAH52390.1.
CCDSiCCDS39577.1. [Q920B0-2]
RefSeqiNP_660130.2. NM_145148.2. [Q920B0-2]
XP_006506067.1. XM_006506004.2. [Q920B0-1]
UniGeneiMm.27789.

3D structure databases

ProteinModelPortaliQ920B0.
SMRiQ920B0. Positions 68-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231236. 2 interactions.
STRINGi10090.ENSMUSP00000108982.

PTM databases

iPTMnetiQ920B0.
PhosphoSiteiQ920B0.

Proteomic databases

EPDiQ920B0.
MaxQBiQ920B0.
PaxDbiQ920B0.
PRIDEiQ920B0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032146; ENSMUSP00000032146; ENSMUSG00000030064. [Q920B0-1]
ENSMUST00000113355; ENSMUSP00000108982; ENSMUSG00000030064. [Q920B0-2]
GeneIDi232288.
KEGGimmu:232288.
UCSCiuc009dat.1. mouse. [Q920B0-1]

Organism-specific databases

CTDi23150.
MGIiMGI:2141794. Frmd4b.

Phylogenomic databases

eggNOGiKOG3529. Eukaryota.
ENOG410XQFP. LUCA.
GeneTreeiENSGT00830000128251.
HOGENOMiHOG000294161.
HOVERGENiHBG062800.
InParanoidiQ920B0.
OMAiNEYRIRC.
OrthoDBiEOG7HF1HH.
PhylomeDBiQ920B0.
TreeFamiTF328984.

Miscellaneous databases

NextBioi381022.
PROiQ920B0.
SOURCEiSearch...

Gene expression databases

BgeeiQ920B0.
CleanExiMM_FRMD4B.
ExpressionAtlasiQ920B0. baseline and differential.
GenevisibleiQ920B0. MM.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR021774. DUF3338.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF11819. DUF3338. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
SMARTiSM00295. B41. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Signaling complexes of the FERM domain-containing protein GRSP1 bound to ARF exchange factor GRP1."
    Klarlund J.K., Holik J., Chawla A., Park J.G., Buxton J., Czech M.P.
    J. Biol. Chem. 276:40065-40070(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1/2), INTERACTION WITH GRP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain and Lung.
  2. "Mus musculus mRNA for Golgi-associated band 4.1-like protein, Goblin, complete cds."
    Watanabe N.M., Kusumi A., Dan I.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: ICR.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Placenta.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Embryonic brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372 AND SER-698, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiFRM4B_MOUSE
AccessioniPrimary (citable) accession number: Q920B0
Secondary accession number(s): Q3V1S1, Q920B1, Q9ESP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: April 5, 2011
Last modified: May 11, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.