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Protein

FERM domain-containing protein 4B

Gene

Frmd4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of GRP1 signaling complexes that are acutely recruited to plasma membrane ruffles in response to insulin receptor signaling. May function as a scaffolding protein that regulates epithelial cell polarity by connecting ARF6 activation with the PAR3 complex (PubMed:20080746). Plays a redundant role with FRMD4A in epithelial polarization (PubMed:20080746).1 Publication

GO - Biological processi

  • establishment of epithelial cell polarity Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
FERM domain-containing protein 4B
Alternative name(s):
GRP1-binding protein GRSP1
Golgi-associated band 4.1-like protein
Short name:
GOBLIN
Gene namesi
Name:Frmd4b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2141794 Frmd4b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194471 – 1035FERM domain-containing protein 4BAdd BLAST1035

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei372PhosphoserineCombined sources1
Modified residuei609PhosphoserineBy similarity1
Modified residuei698PhosphoserineCombined sources1
Cross-linki883Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei916PhosphoserineBy similarity1
Cross-linki1030Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ920B0
PRIDEiQ920B0

PTM databases

iPTMnetiQ920B0
PhosphoSitePlusiQ920B0

Expressioni

Tissue specificityi

Isoform 1 is expressed in the brain. Isoform 2 is expressed in the lung (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030064
CleanExiMM_FRMD4B
ExpressionAtlasiQ920B0 baseline and differential
GenevisibleiQ920B0 MM

Interactioni

Subunit structurei

Interacts with CYTH3 (PubMed:11445584, PubMed:20080746). Interacts with PARD3 (PubMed:20080746). Interacts with CYTH1.2 Publications

Protein-protein interaction databases

BioGridi231236, 2 interactors
STRINGi10090.ENSMUSP00000108982

Structurei

3D structure databases

ProteinModelPortaliQ920B0
SMRiQ920B0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 361FERMPROSITE-ProRule annotationAdd BLAST303

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni542 – 972Necessary for adherens junction and tight junction localization1 PublicationAdd BLAST431

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili414 – 451Sequence analysisAdd BLAST38
Coiled coili535 – 559Sequence analysisAdd BLAST25

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3529 Eukaryota
ENOG410XQFP LUCA
GeneTreeiENSGT00890000139341
HOGENOMiHOG000294161
HOVERGENiHBG062800
InParanoidiQ920B0
OMAiQHCRQRS
OrthoDBiEOG091G0B4Y
PhylomeDBiQ920B0
TreeFamiTF328984

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR021774 DUF3338
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR019747 FERM_CS
IPR000299 FERM_domain
IPR018979 FERM_N
IPR018980 FERM_PH-like_C
IPR011993 PH-like_dom_sf
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF11819 DUF3338, 1 hit
PF09380 FERM_C, 1 hit
PF00373 FERM_M, 1 hit
PF09379 FERM_N, 1 hit
PRINTSiPR00935 BAND41
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM01196 FERM_C, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q920B0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVFMCGVE DLLFSGSRFV WNLTVSTLRR WYTERLRACH QVLRTWCGLR
60 70 80 90 100
DVYQMTEGRH CQVHLLDDRR LELLVQPKLL SRELLDLVAS HFNLKEKEYF
110 120 130 140 150
GITFIDDTGQ ENWLQLDHRV LEHDLPKKPG PTLLHFAVRF YIESISFLKD
160 170 180 190 200
KNTVELFFLN AKACVHKGQI EVDSETIFKL AALVLQESKG DYTSDENARK
210 220 230 240 250
DLKTLPVFPT KTLQEHPSLA YCEDRVIEHY LKIKGLTRGQ AVVQYMKIVE
260 270 280 290 300
ALPTYGVHYY AVKDKQGLPW WLGISYKGIG QYDLQDKVKP RKLFQWKQLE
310 320 330 340 350
NLYFREKKFA VEVHDPRRIS VSRRTFGQSG LFVQTWYANS SLIKSIWVMA
360 370 380 390 400
ISQHQFYLDR KQSKAKIPSA RSLDDIAMDL TETGTQRGSK LVTLEAKSQF
410 420 430 440 450
IMASNGSLIS SGSQDSEGME EQKREKILEL KKKEKLLQEK LLQKVEELKK
460 470 480 490 500
ICLREAELTG RMPKEYPLNI GEKPPQVRRR VGTTFKLDDN LLPTEEDPAL
510 520 530 540 550
QELESNFLIQ QKLVEAAKKL ASEPDLCKTV KKKRKQDYTD AVKRLQEIEN
560 570 580 590 600
SINEYRIRCG KKPSQKAAVV PPEDIIPSES SSLSDTTTYD DPNDSFTLAG
610 620 630 640 650
QRPSSVPHSP RILPPKSLGI ERIHFRKSSI NEQFMDTRHS REMLSTHSSP
660 670 680 690 700
YKTLERRPQG GRSMPTTPVL TRNAYSSSHL EPDSSSQHCR QRSGSLESQS
710 720 730 740 750
HLLSEMDSDK PFFTLSKSQR SSSTEILDDG SSYTSQSSSE YYCVTPAASP
760 770 780 790 800
YYTTQTLDTR ARGRRRSKKH SVSTSNSGSM PNLAQKDPLR NGVYSKGQDP
810 820 830 840 850
PPSGYYIAGY PPYAECDLYY SGGYVYENDT EGQYSVNPSY RSSAHYGYDR
860 870 880 890 900
QRDYSRSFHE DEVDRVPHNP YATLRLPRKA AVKSEHITKN IHKALVAEHL
910 920 930 940 950
RGWYQRASGQ KDQGHSPQTS FDSDRGSQRC LGFAGLQVPC SPSSRASSYS
960 970 980 990 1000
SVSSTNASGN WRTQLTIGLS EYENPVHSPY TSYYGSIYNP LSSPSRQYAE
1010 1020 1030
TTPLDGTDGS QLEDNLEGSE QRLFWHEDSK PGTLV
Length:1,035
Mass (Da):118,007
Last modified:April 5, 2011 - v2
Checksum:i5CA90A96E3F3368A
GO
Isoform 2 (identifier: Q920B0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Show »
Length:981
Mass (Da):111,566
Checksum:iE8332CB283CF829A
GO

Sequence cautioni

The sequence AAL26916 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62Q → R in BAB17031 (Ref. 2) Curated1
Sequence conflicti224D → G in BAE21079 (PubMed:16141072).Curated1
Sequence conflicti802P → S in BAB17031 (Ref. 2) Curated1
Sequence conflicti917 – 918PQ → TK in BAB17031 (Ref. 2) Curated2
Sequence conflicti986S → N in BAB17031 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0408961 – 54Missing in isoform 2. 3 PublicationsAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF327856 mRNA Translation: AAL26916.1 Different initiation.
AF327857 mRNA Translation: AAL26917.1
AB042027 mRNA Translation: BAB17031.1
AK132280 mRNA Translation: BAE21079.1
BC052390 mRNA Translation: AAH52390.1
CCDSiCCDS39577.1 [Q920B0-2]
CCDS85102.1 [Q920B0-1]
RefSeqiNP_001333566.1, NM_001346637.1 [Q920B0-1]
NP_001333568.1, NM_001346639.1
NP_660130.2, NM_145148.2 [Q920B0-2]
XP_017177026.1, XM_017321537.1 [Q920B0-2]
UniGeneiMm.27789

Genome annotation databases

EnsembliENSMUST00000032146; ENSMUSP00000032146; ENSMUSG00000030064 [Q920B0-1]
ENSMUST00000113355; ENSMUSP00000108982; ENSMUSG00000030064 [Q920B0-2]
GeneIDi232288
KEGGimmu:232288
UCSCiuc009dat.1 mouse [Q920B0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFRM4B_MOUSE
AccessioniPrimary (citable) accession number: Q920B0
Secondary accession number(s): Q3V1S1, Q920B1, Q9ESP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: April 5, 2011
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health