Q920A7 (AFG31_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AFG3-like protein 1
EC=3.4.24.-

Gene names

Name:

Afg3l1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	789 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Putative ATP-dependent protease.
Cofactor	Binds 1 zinc ion per subunit Potential.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the AAA ATPase family. In the C-terminal section; belongs to the peptidase M41 family.
Caution	The orthologous human gene is a pseudogene.
Sequence caution	The sequence AAK66971.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB28211.3 differs from that shown. Reason: Frameshift at position 599.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cristae formation Inferred from genetic interaction PubMed 20038678. Source: MGI mitochondrial fusion Inferred from genetic interaction PubMed 20038678. Source: MGI mitochondrial protein processing Inferred from genetic interaction PubMed 20038678. Source: MGI protein catabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay Ref.3PubMed 14651853PubMed 18614015. Source: MGI
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from sequence alignment Ref.3. Source: MGI nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q920A7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q920A7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     548-584: LEKKTQVLQPSEKTTVAYHEAGHAVVGWFLEHADPLL → VHHTSRQGAWLRPVPSPRAVPLHTRAALRPHVYDAGG
     585-789: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 789	789	AFG3-like protein 1		PRO_0000084672
Regions
	Transmembrane	139 – 158	20	Helical; Potential
	Transmembrane	246 – 264	19	Helical; Potential
	Nucleotide binding	340 – 347	8	ATP Potential
Sites
	Active site	567	1	By similarity
	Metal binding	566	1	Zinc; catalytic By similarity
	Metal binding	570	1	Zinc; catalytic By similarity
	Metal binding	641	1	Zinc; catalytic By similarity
Natural variations
	Alternative sequence	548 – 584	37	LEKKT…ADPLL → VHHTSRQGAWLRPVPSPRAV PLHTRAALRPHVYDAGG in isoform 2.		VSP_031809
	Alternative sequence	585 – 789	205	Missing in isoform 2.		VSP_031810

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 06B23E0176285346	FASTA	789	87,047
	10 20 30 40 50 60 MLLRLVGAAG SRALAWPFSK LWRCGGCAGS GGTVWSSVRA CGIALQGHLG RCSQQLALQG 70 80 90 100 110 120 KLTSFSPRLY SKPPRGFEKF FKNKKNRKSA SPGNSVPPKK EPKNAGPGGD GGNRGGKGDD 130 140 150 160 170 180 FPWWKRMQKG EFPWDDKDFR SLAVLGAGVA AGFLYFYFRD PGKEITWKHF VQYYLARGLV 190 200 210 220 230 240 DRLEVVNKQF VRVIPVPGTT SERFVWFNIG SVDTFERNLE SAQWELGIEP TNQAAVVYTT 250 260 270 280 290 300 ESDGSFLRSL VPTLVLVSIL LYAMRRGPMG TGRGGRGGGL FSVGETTAKI LKNNIDVRFA 310 320 330 340 350 360 DVAGCEEAKL EIMEFVNFLK NPKQYQDLGA KIPKGAMLTG PPGTGKTLLA KATAGEANVP 370 380 390 400 410 420 FITVNGSEFL EMFVGVGPAR VRDMFAMARK HAPCILFIDE IDAIGRKRGR GHLGGQSEQE 430 440 450 460 470 480 NTLNQMLVEM DGFNSSTNVV VLAGTNRPDI LDPALTRPGR FDRQIYIGPP DIKGRSSIFK 490 500 510 520 530 540 VHLRPLKLDG SLSKDALSRK LAALTPGFTG ADISNVCNEA ALIAARHLSP SVQERHFEQA 550 560 570 580 590 600 IERVIGGLEK KTQVLQPSEK TTVAYHEAGH AVVGWFLEHA DPLLKVSIIP RGKGLGYAQY 610 620 630 640 650 660 LPREQFLYTR EQLFDRMCMM LGGRVAEQLF FGQITTGAQD DLRKVTQSAY AQIVQFGMSE 670 680 690 700 710 720 KLGQVSFDFP RQGETMVEKP YSEATAQLID EEVRCLVRSA YNRTLELLTQ CREQVEKVGR 730 740 750 760 770 780 RLLEKEVLEK ADMIELLGPR PFAEKSTYEE FVEGTGSLEE DTSLPEGLKD WNKGREEGGT ERGLQESPV
	Isoform 2 [UniParc]. Checksum: 92B8935E7A9FDAD9	FASTA	584	63,639
	10 20 30 40 50 60 MLLRLVGAAG SRALAWPFSK LWRCGGCAGS GGTVWSSVRA CGIALQGHLG RCSQQLALQG 70 80 90 100 110 120 KLTSFSPRLY SKPPRGFEKF FKNKKNRKSA SPGNSVPPKK EPKNAGPGGD GGNRGGKGDD 130 140 150 160 170 180 FPWWKRMQKG EFPWDDKDFR SLAVLGAGVA AGFLYFYFRD PGKEITWKHF VQYYLARGLV 190 200 210 220 230 240 DRLEVVNKQF VRVIPVPGTT SERFVWFNIG SVDTFERNLE SAQWELGIEP TNQAAVVYTT 250 260 270 280 290 300 ESDGSFLRSL VPTLVLVSIL LYAMRRGPMG TGRGGRGGGL FSVGETTAKI LKNNIDVRFA 310 320 330 340 350 360 DVAGCEEAKL EIMEFVNFLK NPKQYQDLGA KIPKGAMLTG PPGTGKTLLA KATAGEANVP 370 380 390 400 410 420 FITVNGSEFL EMFVGVGPAR VRDMFAMARK HAPCILFIDE IDAIGRKRGR GHLGGQSEQE 430 440 450 460 470 480 NTLNQMLVEM DGFNSSTNVV VLAGTNRPDI LDPALTRPGR FDRQIYIGPP DIKGRSSIFK 490 500 510 520 530 540 VHLRPLKLDG SLSKDALSRK LAALTPGFTG ADISNVCNEA ALIAARHLSP SVQERHFEQA 550 560 570 580 IERVIGGVHH TSRQGAWLRP VPSPRAVPLH TRAALRPHVY DAGG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: BALB/c, C57BL/6J and DBA/2. Tissue: Embryo, Erythroleukemia and Thymic lymphoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[3]	"Molecular and functional analyses of the human and mouse genes encoding AFG3L1, a mitochondrial metalloprotease homologous to the human spastic paraplegia protein." Kremmidiotis G., Gardner A.E., Settasatian C., Savoia A., Sutherland G.R., Callen D.F. Genomics 76:58-65(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-789 (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK012394 mRNA. Translation: BAB28211.3. Frameshift. AK159647 mRNA. Translation: BAE35259.1. AK167964 mRNA. Translation: BAE39961.1. AK168244 mRNA. Translation: BAE40194.1. BC056978 mRNA. Translation: AAH56978.1. AF329695 mRNA. Translation: AAK66971.1. Different initiation.
IPI	IPI00468514. IPI00652659.
RefSeq	NP_473411.2. NM_054070.3.
UniGene	Mm.287475.
3D structure databases
ProteinModelPortal	Q920A7.
SMR	Q920A7. Positions 162-244, 288-728.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q920A7. 1 interaction.
STRING	10090.ENSMUSP00000001520.
Protein family/group databases
MEROPS	M41.016.
PTM databases
PhosphoSite	Q920A7.
Proteomic databases
PaxDb	Q920A7.
PRIDE	Q920A7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000001520; ENSMUSP00000001520; ENSMUSG00000031967. ENSMUST00000098320; ENSMUSP00000095924; ENSMUSG00000031967.
GeneID	114896.
KEGG	mmu:114896.
UCSC	uc009nwd.2. mouse.
Organism-specific databases
CTD	114896.
MGI	MGI:1928277. Afg3l1.
Phylogenomic databases
eggNOG	COG0465.
GeneTree	ENSGT00530000063070.
HOGENOM	HOG000217277.
HOVERGEN	HBG050184.
InParanoid	Q920A7.
KO	K08956.
OMA	WDEKDFR.
OrthoDB	EOG4SBDXC.
Gene expression databases
Bgee	Q920A7.
CleanEx	MM_AFG3L1.
Genevestigator	Q920A7.
GermOnline	ENSMUSG00000031967. Mus musculus.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR005936. FtsH. IPR011546. Pept_M41_FtsH_extracell. IPR000642. Peptidase_M41. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF06480. FtsH_ext. 1 hit. PF01434. Peptidase_M41. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01241. FtsH_fam. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	368917.
SOURCE	Search...

Entry information

Entry name

AFG31_MOUSE

Accession

Primary (citable) accession number: Q920A7
Secondary accession number(s): Q3THK2, Q6PGJ7, Q9CZN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program