Reviewed,
UniProtKB/Swiss-Prot Q92091 (NOS2_ONCMY)
Last modified
June 16, 2009.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nitric oxide synthase, inducible EC=1.14.13.39 Alternative name(s): Inducible NO synthase Short name=Inducible NOS Short name=iNOS NOS type II | ||
| Gene names |
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| Organism | Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) | ||
| Taxonomic identifier | 8022 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Oncorhynchus |
Protein attributes
| Sequence length | 470 AA. |
| Sequence status | Fragment. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. |
| Catalytic activity | L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+. |
| Cofactor | Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity. |
| Enzyme regulation | Not stimulated by calcium/calmodulin By similarity. |
| Subunit structure | Homodimer By similarity. |
| Sequence similarities | Belongs to the NOS family. Contains 1 flavodoxin-like domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | FMN binding Inferred from electronic annotation. Source: InterPro calmodulin bindingInferred from electronic annotation. Source: UniProtKB-KW electron carrier activityInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | ‹1 – ›470 | ›470 | Nitric oxide synthase, inducible | PRO_0000170939 | |||||
Regions | |||||||||
| Domain | 169 – 307 | 139 | Flavodoxin-like | ||||||
| Nucleotide binding | 253 – 284 | 32 | FMN By similarity | ||||||
| Nucleotide binding | 398 – 409 | 12 | FAD By similarity | ||||||
| Region | 139 – 159 | 21 | Calmodulin-binding Potential | ||||||
Experimental info | |||||||||
| Non-terminal residue | 1 | 1 | |||||||
| Non-terminal residue | 470 | 1 | |||||||
Sequences
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References
| [1] | "Detection of mRNA for a nitric oxide synthase in macrophages and gill of rainbow trout challenged with an attenuated bacterial pathogen." Grabowski P.S., Laing K.J., Hardie L., Macguigan F., Ralston S., Secombes C.J. (In) Moncada S., Stamler J., Gross S., Higgs E.A. (eds.); 4th International meeting on the biology of nitric oxide, Amelia Island, Florida, Sep. 1995, pp.10:48-48, Portland Press, Brookfield (1996) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| X97013 mRNA. Translation: CAA65736.1. | |
| UniGene | Omy.30 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1NOS based on UniProtKB P29477. |
| SMR | Q92091. Positions 1-132. |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | Q92091. |
Enzyme and pathway databases | |
| BRENDA | 1.14.13.39. 3435. |
Family and domain databases | |
| InterPro | IPR003097. FAD-binding_1. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR004030. NO_synthase_oxygenase_reg. [Graphical view] |
| Gene3D | G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit. |
| Pfam | PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view] |
| PRINTS | PR00369. FLAVODOXIN. |
| PROSITE | PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. Partial match. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NOS2_ONCMY | ||||||||
| Accession | Primary (citable) accession number: Q92091 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||

Clusters with


