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Protein

Nitric oxide synthase, inducible

Gene

nos2

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Not stimulated by calcium/calmodulin.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi253 – 284FMNPROSITE-ProRule annotationAdd BLAST32
Nucleotide bindingi398 – 409FADBy similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

SABIO-RKiQ92091.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:nos2
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000170939‹1 – ›470Nitric oxide synthase, inducibleAdd BLAST›470

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ92091.
SMRiQ92091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini169 – 307Flavodoxin-likePROSITE-ProRule annotationAdd BLAST139

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 159Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

HOVERGENiHBG104152.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiView protein in InterPro
IPR003097. FAD-binding_1.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like_dom.
IPR004030. NOS_N.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS50902. FLAVODOXIN_LIKE. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q92091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GWYMGTEIGV RDFCDYQRYN ILEEVGRRMG LETHKLSSLW KDQALVTINV
60 70 80 90 100
AVTYSFQKNK VIITDHHSAA ESFMKHLETE FPPARRLPAD WDWLVPPMSG
110 120 130 140 150
SLTPIFHQEM VNYILSPFFY YQPDPWMTHV WRNGEMCLKK QQISFKAVAR
160 170 180 190 200
AALFSSTLMS RVLANRVRCT VLYATETGKS QTLAQRLNSM LNCAFNSRLL
210 220 230 240 250
CMEDYNFSDM EQESLLVVVM STFGNGGSPG NGESFKKQLF SLQYLRNKSR
260 270 280 290 300
YCVFGLGSRM YPQFCAFAHA VDAKLEELGA ERVTPTGEGD ELNGQEEAFS
310 320 330 340 350
AWALTALKDA YKEFKIQGQL SLQLPGAERF CEAWDPLRHR VAVESCPQDR
360 370 380 390 400
ITALSAIHSK AVLPMKLKSK HNLQSPQSSR STILVELERE RSPEVMDFAP
410 420 430 440 450
GDHVGVFPGN LPQLVAGILK FLPQTPPTNQ CLRLGYRSDT FRVMRKTGRL
460 470
LDASQHSLCL RHSPTSWTLP
Length:470
Mass (Da):53,329
Last modified:November 1, 1996 - v1
Checksum:i40B6717EE500B64D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-terminal residuei4701

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97013 mRNA. Translation: CAA65736.1.
UniGeneiOmy.30.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNOS2_ONCMY
AccessioniPrimary (citable) accession number: Q92091
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 10, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families