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Q92091 (NOS2_ONCMY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:nos2
OrganismOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifier8022 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiEuteleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Protein attributes

Sequence length470 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Not stimulated by calcium/calmodulin By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the NOS family.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›470›470Nitric oxide synthase, inducible
PRO_0000170939

Regions

Domain169 – 307139Flavodoxin-like
Nucleotide binding253 – 28432FMN By similarity
Nucleotide binding398 – 40912FAD By similarity
Region139 – 15921Calmodulin-binding Potential

Experimental info

Non-terminal residue11
Non-terminal residue4701

Sequences

Sequence LengthMass (Da)Tools
Q92091 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 40B6717EE500B64D

FASTA47053,329
        10         20         30         40         50         60 
GWYMGTEIGV RDFCDYQRYN ILEEVGRRMG LETHKLSSLW KDQALVTINV AVTYSFQKNK 

        70         80         90        100        110        120 
VIITDHHSAA ESFMKHLETE FPPARRLPAD WDWLVPPMSG SLTPIFHQEM VNYILSPFFY 

       130        140        150        160        170        180 
YQPDPWMTHV WRNGEMCLKK QQISFKAVAR AALFSSTLMS RVLANRVRCT VLYATETGKS 

       190        200        210        220        230        240 
QTLAQRLNSM LNCAFNSRLL CMEDYNFSDM EQESLLVVVM STFGNGGSPG NGESFKKQLF 

       250        260        270        280        290        300 
SLQYLRNKSR YCVFGLGSRM YPQFCAFAHA VDAKLEELGA ERVTPTGEGD ELNGQEEAFS 

       310        320        330        340        350        360 
AWALTALKDA YKEFKIQGQL SLQLPGAERF CEAWDPLRHR VAVESCPQDR ITALSAIHSK 

       370        380        390        400        410        420 
AVLPMKLKSK HNLQSPQSSR STILVELERE RSPEVMDFAP GDHVGVFPGN LPQLVAGILK 

       430        440        450        460        470 
FLPQTPPTNQ CLRLGYRSDT FRVMRKTGRL LDASQHSLCL RHSPTSWTLP 

« Hide

References

[1]"Detection of mRNA for a nitric oxide synthase in macrophages and gill of rainbow trout challenged with an attenuated bacterial pathogen."
Grabowski P.S., Laing K.J., Hardie L., Macguigan F., Ralston S., Secombes C.J.
(In) Moncada S., Stamler J., Gross S., Higgs E.A. (eds.); 4th International meeting on the biology of nitric oxide, Amelia Island, Florida, Sep. 1995, pp.10:48-48, Portland Press, Brookfield (1996)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97013 mRNA. Translation: CAA65736.1.
UniGeneOmy.30.

3D structure databases

ProteinModelPortalQ92091.
SMRQ92091. Positions 1-132.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG104152.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERPTHR19384:SF5. PTHR19384:SF5. 1 hit.
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PRINTSPR00369. FLAVODOXIN.
SUPFAMSSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS2_ONCMY
AccessionPrimary (citable) accession number: Q92091
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families