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Protein

Cytochrome P450 2K1

Gene

cyp2k1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2K1 (EC:1.14.14.1)
Alternative name(s):
CYPIIK1
Cytochrome P450 LMC2
Gene namesi
Name:cyp2k1
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Cytochrome P450 2K1PRO_0000051773Add
BLAST

Expressioni

Inductioni

Constitutively expressed.

Structurei

3D structure databases

ProteinModelPortaliQ92090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG015789.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92090-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLIEDILQT SSTVTLLGTV LFLLVLYLRS SGSSSEEQGK EPPGPRPLPL
60 70 80 90 100
LGNMLQLDLK KPYCTLCELS KKYGSIFTVH FGPKKVVVLA GYKTVKQALV
110 120 130 140 150
NQAEDFGDRD ITPVFYDFNQ GHGILFANGD SWKEMRRFAL TNLRDFGMGK
160 170 180 190 200
KGSEEKILEE IPYLIEVFEK HEGKAFDTTQ SVLYAVSNII SAIVYGSRFE
210 220 230 240 250
YTDPLFTGMA DRAKESIHLT GSASIQMYNM FPWLGPWINN LTRLKKNIAD
260 270 280 290 300
MKMEVIELVR GLKETLNPHM CRGFVDSFLV RKQTLEESGH MDSFYHDDNL
310 320 330 340 350
VFSVGNLFSA GTDTTGTTLR WGLLLMTKYP HIQDQVQEEI SRVIGSRQTL
360 370 380 390 400
VEDRKNLPYT DAVIHETQRL ANIVPMSVPH TTSRDVTFQG YFIKKGTSVI
410 420 430 440 450
PLLTSVLQDD SEWESPNTFN PSHFLDEQGG FVKRDAFMAF SAGRRVCLGE
460 470 480 490 500
GLARMELFLF FTSLLQRFRF SPPPGVTEDD LDLTPLLGFT LNPSPHQLCA

VSRV
Length:504
Mass (Da):56,792
Last modified:November 1, 1996 - v1
Checksum:i3EFAE8D78D5EB21F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti6 – 72DI → GL in allele CYP2K1v2.
Natural varianti37 – 371E → G in allele CYP2K1v2.
Natural varianti79 – 791V → F in allele CYP2K1v2.
Natural varianti256 – 2561I → T in allele CYP2K1v2.
Natural varianti261 – 2611G → S in allele CYP2K1v2.
Natural varianti372 – 3721N → D in allele CYP2K1v2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11528 mRNA. Translation: AAA52078.1.
AF045052 mRNA. Translation: AAC28309.1.
PIRiS45644.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11528 mRNA. Translation: AAA52078.1.
AF045052 mRNA. Translation: AAC28309.1.
PIRiS45644.

3D structure databases

ProteinModelPortaliQ92090.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG015789.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of the major rainbow trout constitutive cytochrome P450 (CYP2K1): identification of a new cytochrome P450 gene subfamily and its expression in mature rainbow trout liver and trunk kidney."
    Buhler D.R., Yang Y.-H., Dreher T.W., Miranda C.L., Wang J.-L.
    Arch. Biochem. Biophys. 312:45-51(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Cloning and sequencing of new cytochromes P450 from liver of rainbow trout."
    Yang Y.-H., Buhler D.R.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiCP2K1_ONCMY
AccessioniPrimary (citable) accession number: Q92090
Secondary accession number(s): O93304
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.