Q92090 (CP2K1_ONCMY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2K1
EC=1.14.14.1

Alternative name(s):
CYPIIK1
Cytochrome P450 LMC2

Gene names

Name:

cyp2k1

Organism

Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Taxonomic identifier

8022 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonoidei › Salmonidae › Salmoninae › Oncorhynchus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Microsome membrane; Peripheral membrane protein By similarity.
Induction	Constitutively expressed.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Cytochrome P450 2K1		PRO_0000051773
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	6 – 7	2	DI → GL in allele CYP2K1v2.
	Natural variant	37	1	E → G in allele CYP2K1v2.
	Natural variant	79	1	V → F in allele CYP2K1v2.
	Natural variant	256	1	I → T in allele CYP2K1v2.
	Natural variant	261	1	G → S in allele CYP2K1v2.
	Natural variant	372	1	N → D in allele CYP2K1v2.

Sequences

	Sequence		Length	Mass (Da)
	Q92090 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3EFAE8D78D5EB21F	FASTA	504	56,792
	10 20 30 40 50 60 MSLIEDILQT SSTVTLLGTV LFLLVLYLRS SGSSSEEQGK EPPGPRPLPL LGNMLQLDLK 70 80 90 100 110 120 KPYCTLCELS KKYGSIFTVH FGPKKVVVLA GYKTVKQALV NQAEDFGDRD ITPVFYDFNQ 130 140 150 160 170 180 GHGILFANGD SWKEMRRFAL TNLRDFGMGK KGSEEKILEE IPYLIEVFEK HEGKAFDTTQ 190 200 210 220 230 240 SVLYAVSNII SAIVYGSRFE YTDPLFTGMA DRAKESIHLT GSASIQMYNM FPWLGPWINN 250 260 270 280 290 300 LTRLKKNIAD MKMEVIELVR GLKETLNPHM CRGFVDSFLV RKQTLEESGH MDSFYHDDNL 310 320 330 340 350 360 VFSVGNLFSA GTDTTGTTLR WGLLLMTKYP HIQDQVQEEI SRVIGSRQTL VEDRKNLPYT 370 380 390 400 410 420 DAVIHETQRL ANIVPMSVPH TTSRDVTFQG YFIKKGTSVI PLLTSVLQDD SEWESPNTFN 430 440 450 460 470 480 PSHFLDEQGG FVKRDAFMAF SAGRRVCLGE GLARMELFLF FTSLLQRFRF SPPPGVTEDD 490 500 LDLTPLLGFT LNPSPHQLCA VSRV

References

[1]

"Cloning and sequencing of the major rainbow trout constitutive cytochrome P450 (CYP2K1): identification of a new cytochrome P450 gene subfamily and its expression in mature rainbow trout liver and trunk kidney." Buhler D.R., Yang Y.-H., Dreher T.W., Miranda C.L., Wang J.-L. Arch. Biochem. Biophys. 312:45-51(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

[2]

"Cloning and sequencing of new cytochromes P450 from liver of rainbow trout." Yang Y.-H., Buhler D.R. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11528 mRNA. Translation: AAA52078.1. AF045052 mRNA. Translation: AAC28309.1.
PIR	S45644.
3D structure databases
ProteinModelPortal	Q92090.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2K1_ONCMY

Accession

Primary (citable) accession number: Q92090
Secondary accession number(s): O93304

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program