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Q92088

- CP2M1_ONCMY

UniProt

Q92088 - CP2M1_ONCMY

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Protein

Cytochrome P450 2M1

Gene

cyp2m1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Has (omega-6)-hydroxylation activity toward lauric acid.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi441 – 4411Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2M1 (EC:1.14.14.1)
Alternative name(s):
CYPIIM1
LMC1
Lauric acid omega-6-hydroxylase
Gene namesi
Name:cyp2m1
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Cytochrome P450 2M1PRO_0000051777Add
BLAST

Expressioni

Tissue specificityi

In kidney and in liver from juvenile and sexually mature trout from both sexes.

Structurei

3D structure databases

ProteinModelPortaliQ92088.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG015789.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92088-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDVLHILQTN FVSIIIGFVV IILLWMNRGK QSNSRLPPGP APIPLLGNLL
60 70 80 90 100
RMDVKAPYKL YMELSKKYGS VFTVWLGSKP VVVISGYQAI KDAFVTQGEE
110 120 130 140 150
FSGRANYPVI MTVSKGYGVL VSSGKRSKDL RRFSLMTLKT FGMGRRSIEE
160 170 180 190 200
RVQEEAKMLV KAFGEYRDSV VNPKELLCNC VGNVICSIVF GHRFENDDPM
210 220 230 240 250
FQLIQKAVDA YFNVLSSPIG AMYNMFPRIV WCFPGNHHEM FAIVNKAKVY
260 270 280 290 300
IQEQAEIRLK TLNISEPQDF IEAFLVKMLE EKDDPNTEFN NGNMVMTAWS
310 320 330 340 350
LFAAGTETTS STLRQSFLMM IKYPHIQESV QKEIDEVIGS RVPTVDDRVK
360 370 380 390 400
MPYTDAVIHE VQRYMDLSPT SVPHKVMRDT EFYNYHIPEG TMVLPLLSSV
410 420 430 440 450
LVDPKLFKNP DEFDPENFLD ENGVFKKNDG FFAFGVGKRA CPGEALARVE
460 470 480 490
LFLFFTSVLQ RFTFTGTKPP EEINIEPACS SFGRLPRSYD CYIKLRTEK
Length:499
Mass (Da):56,773
Last modified:November 1, 1996 - v1
Checksum:i2D5C106C192ECBA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16657 mRNA. Translation: AAA62499.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16657 mRNA. Translation: AAA62499.1 .

3D structure databases

ProteinModelPortali Q92088.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG015789.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "CYP2M1: cloning, sequencing, and expression of a new cytochrome P450 from rainbow trout liver with fatty acid (omega-6)-hydroxylation activity."
    Yang Y.-H., Wang J.-L., Miranda C.L., Buhler D.R.
    Arch. Biochem. Biophys. 352:271-280(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Shasta.
    Tissue: Liver.
  2. "Regiospecificity in the hydroxylation of lauric acid by rainbow trout hepatic cytochrome P450 isozymes."
    Miranda C.L., Wang J.L., Henderson M.C., Williams D.E., Buhler D.R.
    Biochem. Biophys. Res. Commun. 171:537-542(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiCP2M1_ONCMY
AccessioniPrimary (citable) accession number: Q92088
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3