Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q92088

- CP2M1_ONCMY

UniProt

Q92088 - CP2M1_ONCMY

Protein

Cytochrome P450 2M1

Gene

cyp2m1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Has (omega-6)-hydroxylation activity toward lauric acid.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi441 – 4411Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2M1 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIM1
    LMC1
    Lauric acid omega-6-hydroxylase
    Gene namesi
    Name:cyp2m1
    OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
    Taxonomic identifieri8022 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Cytochrome P450 2M1PRO_0000051777Add
    BLAST

    Expressioni

    Tissue specificityi

    In kidney and in liver from juvenile and sexually mature trout from both sexes.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92088.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    HOVERGENiHBG015789.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92088-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVLHILQTN FVSIIIGFVV IILLWMNRGK QSNSRLPPGP APIPLLGNLL    50
    RMDVKAPYKL YMELSKKYGS VFTVWLGSKP VVVISGYQAI KDAFVTQGEE 100
    FSGRANYPVI MTVSKGYGVL VSSGKRSKDL RRFSLMTLKT FGMGRRSIEE 150
    RVQEEAKMLV KAFGEYRDSV VNPKELLCNC VGNVICSIVF GHRFENDDPM 200
    FQLIQKAVDA YFNVLSSPIG AMYNMFPRIV WCFPGNHHEM FAIVNKAKVY 250
    IQEQAEIRLK TLNISEPQDF IEAFLVKMLE EKDDPNTEFN NGNMVMTAWS 300
    LFAAGTETTS STLRQSFLMM IKYPHIQESV QKEIDEVIGS RVPTVDDRVK 350
    MPYTDAVIHE VQRYMDLSPT SVPHKVMRDT EFYNYHIPEG TMVLPLLSSV 400
    LVDPKLFKNP DEFDPENFLD ENGVFKKNDG FFAFGVGKRA CPGEALARVE 450
    LFLFFTSVLQ RFTFTGTKPP EEINIEPACS SFGRLPRSYD CYIKLRTEK 499
    Length:499
    Mass (Da):56,773
    Last modified:November 1, 1996 - v1
    Checksum:i2D5C106C192ECBA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16657 mRNA. Translation: AAA62499.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16657 mRNA. Translation: AAA62499.1 .

    3D structure databases

    ProteinModelPortali Q92088.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG015789.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CYP2M1: cloning, sequencing, and expression of a new cytochrome P450 from rainbow trout liver with fatty acid (omega-6)-hydroxylation activity."
      Yang Y.-H., Wang J.-L., Miranda C.L., Buhler D.R.
      Arch. Biochem. Biophys. 352:271-280(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Shasta.
      Tissue: Liver.
    2. "Regiospecificity in the hydroxylation of lauric acid by rainbow trout hepatic cytochrome P450 isozymes."
      Miranda C.L., Wang J.L., Henderson M.C., Williams D.E., Buhler D.R.
      Biochem. Biophys. Res. Commun. 171:537-542(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiCP2M1_ONCMY
    AccessioniPrimary (citable) accession number: Q92088
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3