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Q92088 (CP2M1_ONCMY) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2M1

EC=1.14.14.1
Alternative name(s):
CYPIIM1
LMC1
Lauric acid omega-6-hydroxylase
Gene names
Name:cyp2m1
OrganismOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifier8022 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiEuteleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has (omega-6)-hydroxylation activity toward lauric acid.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Microsome membrane; Peripheral membrane protein By similarity.

Tissue specificity

In kidney and in liver from juvenile and sexually mature trout from both sexes.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Cytochrome P450 2M1
PRO_0000051777

Sites

Metal binding4411Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92088 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2D5C106C192ECBA2

FASTA49956,773
        10         20         30         40         50         60 
MDVLHILQTN FVSIIIGFVV IILLWMNRGK QSNSRLPPGP APIPLLGNLL RMDVKAPYKL 

        70         80         90        100        110        120 
YMELSKKYGS VFTVWLGSKP VVVISGYQAI KDAFVTQGEE FSGRANYPVI MTVSKGYGVL 

       130        140        150        160        170        180 
VSSGKRSKDL RRFSLMTLKT FGMGRRSIEE RVQEEAKMLV KAFGEYRDSV VNPKELLCNC 

       190        200        210        220        230        240 
VGNVICSIVF GHRFENDDPM FQLIQKAVDA YFNVLSSPIG AMYNMFPRIV WCFPGNHHEM 

       250        260        270        280        290        300 
FAIVNKAKVY IQEQAEIRLK TLNISEPQDF IEAFLVKMLE EKDDPNTEFN NGNMVMTAWS 

       310        320        330        340        350        360 
LFAAGTETTS STLRQSFLMM IKYPHIQESV QKEIDEVIGS RVPTVDDRVK MPYTDAVIHE 

       370        380        390        400        410        420 
VQRYMDLSPT SVPHKVMRDT EFYNYHIPEG TMVLPLLSSV LVDPKLFKNP DEFDPENFLD 

       430        440        450        460        470        480 
ENGVFKKNDG FFAFGVGKRA CPGEALARVE LFLFFTSVLQ RFTFTGTKPP EEINIEPACS 

       490 
SFGRLPRSYD CYIKLRTEK 

« Hide

References

[1]"CYP2M1: cloning, sequencing, and expression of a new cytochrome P450 from rainbow trout liver with fatty acid (omega-6)-hydroxylation activity."
Yang Y.-H., Wang J.-L., Miranda C.L., Buhler D.R.
Arch. Biochem. Biophys. 352:271-280(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Shasta.
Tissue: Liver.
[2]"Regiospecificity in the hydroxylation of lauric acid by rainbow trout hepatic cytochrome P450 isozymes."
Miranda C.L., Wang J.L., Henderson M.C., Williams D.E., Buhler D.R.
Biochem. Biophys. Res. Commun. 171:537-542(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16657 mRNA. Translation: AAA62499.1.

3D structure databases

ProteinModelPortalQ92088.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG015789.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP2M1_ONCMY
AccessionPrimary (citable) accession number: Q92088
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: December 11, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families