Reviewed, UniProtKB/Swiss-Prot Q92087 (CP19A_ORYLA)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 19A1
    EC=1.14.14.1
Alternative name(s):
    CYPXIX
    Aromatase
    Estrogen synthase
    Cytochrome P-450AROM

Gene names

Name:	cyp19a1
Synonyms:	cyp19

Organism

Oryzias latipes (Medaka fish) (Japanese ricefish)

Taxonomic identifier

8090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Beloniformes › Adrianichthyidae › Oryziinae › Oryzias

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of aromatic C18 estrogens from C19 androgens By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Cytochrome P450 19A1		PRO_0000051972
Sites
	Metal binding	446	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92087-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 39EFE4C412D2382A	FASTA	518	58,941
	10 20 30 40 50 60 MDLIPACDRT MSSSCLVAEL VSIAPNTTVG LPSGIPMATR SLILLVCLLL MVWSHSEKKT 70 80 90 100 110 120 IPGPSFCLGL GPLMSYLRFI WTGIGTASNY YNNKYGDIVR VWINGEETLI LSRASAVHHV 130 140 150 160 170 180 LKNRKYTSRF GSKQGLSCIG MNEKGIIFNN NVALWKKIRT YFTKALTGPN LQQTVEVCVT 190 200 210 220 230 240 STQTHLDNLS SLSYVDVLGF LRCTVVDISN RLFLGVPVDE KELLQKIHKY FDTWQTVLIK 250 260 270 280 290 300 PDIYFKFSWI HQRHKTAAQE LQDAIESLVE RKRKEMEQAE KLDNINFTAE LIFAQGHGEL 310 320 330 340 350 360 SAENVRQCVL EMVIAAPDTL SISLFFMLLL LKQNPHVELQ LLQEIDTIVG DSQLQNQDLQ 370 380 390 400 410 420 KLQVLESFIN ECLRFHPVVD FTMRRALFDD IIDGHRVQKG TNIILNTGRM HRTEFFHKAN 430 440 450 460 470 480 EFSLENFQKN TPRRYFQPFG SGPRACVGRH IAMVMMKSIL VTLLSQYSVC PHEGLTLDCL 490 500 510 PQTNNLSQQP VEHHQEADHL SMTFLPRQRG IWESPSPF

References

[1]	"Structure and promoter analysis of the cytochrome P-450 aromatase gene of the teleost fish, medaka (Oryzias latipes)." Tanaka M., Fukada S., Matsuyama M., Nagahama Y. J. Biochem. 117:719-725(1995) [PubMed: 7592531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Orange-red. Tissue: Ovarian follicle.
[2]	"Isolation, characterization, and expression of cDNAs encoding the medaka (Oryzias latipes) ovarian follicle cytochrome P-450 aromatase." Fukada S., Tanaka M., Matsuyama M., Kobayashi D., Nagahama Y. Mol. Reprod. Dev. 45:285-290(1996) [PubMed: 8916038] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Ovarian follicle.
[3]	"Construction of a linkage map of the Medaka (Oryzias latipes) and mapping of the Da mutant locus defective in dorsoventral patterning." Ohtsuka M., Makino S., Yoda K., Wada H., Naruse K., Mitani H., Shima A., Ozato K., Kimura M., Inoko H. Genome Res. 9:1277-1287(1999) [PubMed: 10613850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 418-496. Strain: Da and HNI.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D82968 mRNA. Translation: BAA11656.1. D82969 Genomic DNA. Translation: BAA11657.1. AB030454 Genomic DNA. Translation: BAA85121.1. AB030455 Genomic DNA. Translation: BAA85122.1.
3D structure databases
SMR	Q92087. Positions 62-508.
ModBase	Search...
Protein-protein interaction databases
STRING	Q92087.
Genome annotation databases
Ensembl	ENSORLT00000003689; ENSORLP00000003688; ENSORLG00000002949; Oryzias latipes. [Genome view]
Phylogenomic databases
eggNOG	fiNOG06676.
HOVERGEN	Q92087.
InParanoid	Q92087.
Enzyme and pathway databases
BRENDA	1.14.14.1. 19130.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP19A_ORYLA

Accession

Primary (citable) accession number: Q92087
Secondary accession number(s): O13271, O13279, Q9PRF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)