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Protein

Neutral phospholipase A2 muscarinic inhibitor

Gene
N/A
Organism
Naja sputatrix (Malayan spitting cobra) (Naja naja sputatrix)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that inhibits the muscarinic acetylcholine receptors (mAChR/CHRM) with no subtype selectivity. Shows catalytic activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Calcium; via carbonyl oxygenBy similarity
Metal bindingi56 – 561Calcium; via carbonyl oxygenBy similarity
Metal bindingi58 – 581Calcium; via carbonyl oxygenBy similarity
Active sitei74 – 741By similarity
Metal bindingi75 – 751CalciumBy similarity
Active sitei120 – 1201By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled acetylcholine receptor impairing toxin, G-protein coupled receptor impairing toxin, Hydrolase, Toxin

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral phospholipase A2 muscarinic inhibitor (EC:3.1.1.4)
Short name:
NPLA
Short name:
svPLA2
Alternative name(s):
NAJPLA-2A
Phosphatidylcholine 2-acylhydrolase
Phospholipase A2 A
OrganismiNaja sputatrix (Malayan spitting cobra) (Naja naja sputatrix)
Taxonomic identifieri33626 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 2762 PublicationsPRO_0000022930
Chaini28 – 146119Neutral phospholipase A2 muscarinic inhibitorPRO_0000022931Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi38 ↔ 98By similarity
Disulfide bondi53 ↔ 145By similarity
Disulfide bondi55 ↔ 71By similarity
Disulfide bondi70 ↔ 126By similarity
Disulfide bondi77 ↔ 119By similarity
Disulfide bondi87 ↔ 112By similarity
Disulfide bondi105 ↔ 117By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ92084.
SMRiQ92084. Positions 28-146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPAHLLILA AVCVSPLGAS SNRPMPLNLY QFKNMIQCTV PNRSWWHFAD
60 70 80 90 100
YGCYCGRGGS GTPVDDLDRC CQIHDNCYNE AEKISRCWPY FKTYSYECSQ
110 120 130 140
GTLTCKGGNN ACAAAVCDCD RLAAICFAGA PYNDNNYNID LKARCQ
Length:146
Mass (Da):16,189
Last modified:November 1, 1996 - v1
Checksum:iB36CF0BFD7AE295A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42004 mRNA. Translation: AAA66027.1.
AF101236 Genomic DNA. Translation: AAF82187.1.
PIRiS65520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42004 mRNA. Translation: AAA66027.1.
AF101236 Genomic DNA. Translation: AAF82187.1.
PIRiS65520.

3D structure databases

ProteinModelPortaliQ92084.
SMRiQ92084. Positions 28-146.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of cDNAs encoding three isoforms of phospholipase A2 in Malayan spitting cobra (Naja naja sputatrix) venom."
    Armugam A., Earnest L., Chung M.C.M., Gopalakrishnakone P., Tan C.H., Tan N.-H., Jeyaseelan K.
    Toxicon 35:27-37(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-48.
    Tissue: Venom and Venom gland.
  2. "Structure and phylogeny of the venom group I phospholipase A(2) gene."
    Jeyaseelan K., Armugam A., Donghui M., Tan N.-H.
    Mol. Biol. Evol. 17:1010-1021(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. "Phospholipase A2 from Naja naja sputatrix venom is a muscarinic acetylcholine receptor inhibitor."
    Miyoshi S., Tu A.T.
    Arch. Biochem. Biophys. 328:17-25(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-43, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    Tissue: Venom.

Entry informationi

Entry nameiPA2NA_NAJSP
AccessioniPrimary (citable) accession number: Q92084
Secondary accession number(s): Q10756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.