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Protein

Neutral phospholipase A2 muscarinic inhibitor

Gene
N/A
Organism
Naja sputatrix (Malayan spitting cobra) (Naja naja sputatrix)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that inhibits the muscarinic acetylcholine receptors (mAChR/CHRM) with no subtype selectivity. Shows catalytic activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Calcium; via carbonyl oxygenBy similarity1
Metal bindingi56Calcium; via carbonyl oxygenBy similarity1
Metal bindingi58Calcium; via carbonyl oxygenBy similarity1
Active sitei74By similarity1
Metal bindingi75CalciumBy similarity1
Active sitei120By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled acetylcholine receptor impairing toxin, G-protein coupled receptor impairing toxin, Hydrolase, Toxin
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral phospholipase A2 muscarinic inhibitor (EC:3.1.1.4)
Short name:
NPLA
Short name:
svPLA2
Alternative name(s):
NAJPLA-2A
Phosphatidylcholine 2-acylhydrolase
Phospholipase A2 A
OrganismiNaja sputatrix (Malayan spitting cobra) (Naja naja sputatrix)
Taxonomic identifieri33626 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002293022 – 272 Publications6
ChainiPRO_000002293128 – 146Neutral phospholipase A2 muscarinic inhibitorAdd BLAST119

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 98By similarity
Disulfide bondi53 ↔ 145By similarity
Disulfide bondi55 ↔ 71By similarity
Disulfide bondi70 ↔ 126By similarity
Disulfide bondi77 ↔ 119By similarity
Disulfide bondi87 ↔ 112By similarity
Disulfide bondi105 ↔ 117By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ92084.
SMRiQ92084.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiView protein in InterPro
IPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiView protein in Pfam
PF00068. Phospholip_A2_1. 1 hit.
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiView protein in SMART
SM00085. PA2c. 1 hit.
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiView protein in PROSITE
PS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPAHLLILA AVCVSPLGAS SNRPMPLNLY QFKNMIQCTV PNRSWWHFAD
60 70 80 90 100
YGCYCGRGGS GTPVDDLDRC CQIHDNCYNE AEKISRCWPY FKTYSYECSQ
110 120 130 140
GTLTCKGGNN ACAAAVCDCD RLAAICFAGA PYNDNNYNID LKARCQ
Length:146
Mass (Da):16,189
Last modified:November 1, 1996 - v1
Checksum:iB36CF0BFD7AE295A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42004 mRNA. Translation: AAA66027.1.
AF101236 Genomic DNA. Translation: AAF82187.1.
PIRiS65520.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPA2NA_NAJSP
AccessioniPrimary (citable) accession number: Q92084
Secondary accession number(s): Q10756
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 1, 1996
Last modified: March 15, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families