Reviewed, UniProtKB/Swiss-Prot Q92072 (DNMT1_CHICK)

Names and origin

Protein names

Recommended name:
    DNA (cytosine-5)-methyltransferase 1
      Short name=Dnmt1
    EC=2.1.1.37
Alternative name(s):
    MCMT
    DNA methyltransferase GgaI
      Short name=DNA MTase GgaI
      Short name=M.GgaI

Gene names

Name:	DNMT1
Synonyms:	AIM, DNMT

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1537 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development By similarity. Mediates transcriptional repression by direct binding to HDAC2 By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subunit structure	Interacts with PCNA. Ref.2
Subcellular location	Nucleus By similarity.
Tissue specificity	Testis and lung.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 2 BAH domains. Contains 1 CXXC-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Repressor Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription factor binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1537	1537	DNA (cytosine-5)-methyltransferase 1		PRO_0000088037
Regions
	Domain	667 – 791	125	BAH 1
	Domain	883 – 1011	129	BAH 2
	Repeat	1020 – 1021	2	1
	Repeat	1022 – 1023	2	2
	Repeat	1024 – 1025	2	3
	Repeat	1026 – 1027	2	4
	Repeat	1028 – 1029	2	5
	Repeat	1030 – 1031	2	6
	Repeat	1032 – 1033	2	7
	Repeat	1034 – 1035	2	8; approximate
	Zinc finger	558 – 604	47	CXXC-type
	Region	182 – 194	13	Interacts with PCNA
	Region	1020 – 1035	16	8 X 2 AA tandem repeats of K-G
	Compositional bias	129 – 149	21	Poly-Ser
	Compositional bias	217 – 227	11	Poly-Glu
Sites
	Active site	1141	1	By similarity
	Metal binding	263	1	Zinc By similarity
	Metal binding	266	1	Zinc By similarity
	Metal binding	329	1	Zinc By similarity
	Site	420	1	Important for activity By similarity
Amino acid modifications
	Modified residue	420	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	190	1	V → H: No loss of interaction with PCNA. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q92072-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FB7E0B2CD10EA17E	FASTA	1,537	172,907
	10 20 30 40 50 60 MPARSAPPPP ALPPALRRRL RDLERDEDSL SEKETLQEKL RLTRGFLRAE VQRRLSALDA 70 80 90 100 110 120 DVRCRELSEE RYLAKVKALL RRELAAENGD AAKLFSRASN GCAGNGEEEW ERGGRGEDGA 130 140 150 160 170 180 MEVEEAAASS SSSSSSSSSS SSSSSSSSSL LPAPRARKAR RSRSNGESKK SPASSRVTRS 190 200 210 220 230 240 SGRQPTILSV FSKGSTKRKS EEVNGAVKPE VSAEKDEEEE EELEEKEQDE KRIKIETKEG 250 260 270 280 290 300 SEIKDEITQV KTSTPAKTTP PKCVDCRQYL DDPDLKFFQG DPDDALEEPE MLTDERLSIF 310 320 330 340 350 360 DANEDGFESY EDLPQHKVTS FSVYDKRGHL CPFDTGLIER NIELYFSGAV KPIYDDNPCL 370 380 390 400 410 420 DGGVRAKKLG PINAWWITGF DGGEKALIGF TTAFADYILM EPSEEYAPIF ALMQEKIYMS 430 440 450 460 470 480 KIVVEFLQNN RDVSYEDLLN KIETTVPPVG LNFNRFTEDS LLRHAQFVVE QVESYDEAGD 490 500 510 520 530 540 SDEPPVLITP CMRDLIKLAG VTLGKRRAVR RQAIRHPTRI DKDKGPTKAT TTKLVYLIFD 550 560 570 580 590 600 TFFSEQIEKD EREDDKENAM KRRRCGVCEV CQQPECGKCK ACQNMVKFGG SGRSKQACLQ 610 620 630 640 650 660 RRCPNLAVRE ADEDEEVDDN IPEMPSPKKM LQGRKKKQNK SRISWVGEPI KSDGKKDFYQ 670 680 690 700 710 720 RVCIDSETLE VGDCVSVSPD DPTKPLYLAR VTAMWEDSSG QMFHAHWFCP GSDTVLGATS 730 740 750 760 770 780 DPLELFLVDE CEDMQLSYIH GKVNVIYKPP SENWAMEGGL DMEIKMVEDD GRTYFYQMWY 790 800 810 820 830 840 DQEYARFETP PRAQPMEDNK YKFCLSCARL DEVRHKEIPK VAEPLDEGDG KMFYAMATKN 850 860 870 880 890 900 GVQYRVGDSV YLLPEAFSFS MKPASPAKRP KKEAVDEDLY PEHYRKYSEY IKGSNLDAPD 910 920 930 940 950 960 PYRVGRIKEI FCHIRTNGKP NEADIKLRIW KFYRPENTHK SMKATYHADI NLLYWSDEET 970 980 990 1000 1010 1020 TVDFCAVQGR CTVVYGEDLT ESIQDYSAGG LDRFYFLEAY NAKTKSFEDP PNHARSSGNK 1030 1040 1050 1060 1070 1080 GKGKGKGKGK GKGKSSTTCE QSEPEPTELK LPKLRTLDVF SGCGGLSEGF HQAGVSETLW 1090 1100 1110 1120 1130 1140 AIEMWEPAAQ AFRLNNPGTT VFTEDCNVLL KLVMSGEKTN SLGQKLPQKG DVEMLCGGPP 1150 1160 1170 1180 1190 1200 CQGFSGMNRF NSRTYSKFKN SLVVSFLSYC DYYRPRFFLL ENVRNFVSFK RSMVLKLTLR 1210 1220 1230 1240 1250 1260 CLVRMGYQCT FGVLQAGQYG VAQTRRRAIV LAAAPGEKLP MFPEPLHVFA PRACQLSVVV 1270 1280 1290 1300 1310 1320 DDKKFVSNIT RTYSGPFRTI TVRDTMSDLP EIRNGASALE ISYNGEPQSW FQRQIRGSQY 1330 1340 1350 1360 1370 1380 QPILRDHICK DMSALVAARM RHIPLAPGSD WRDLPNIEVR LSDGTSTRKL RYTHHEKKNG 1390 1400 1410 1420 1430 1440 RSSSGALRGV CSCAEGKPCD PADRQFNTLI PWCLPHTGNR HNHWAGLYGR LEWDGFFSTT 1450 1460 1470 1480 1490 1500 VTNPEPMGKQ GRVLHPEQHR VVSVRECARS QGFPDTYRLF GNILDKHRQV GNAVPPPLAK 1510 1520 1530 AIGLEIRACV GARMREESGA AVAPPAPEKM EMTAAAD

References

[1]	"Isolation and expression of a chicken DNA methyltransferase cDNA." Tajima S., Tsuda H., Wakabayashi N., Asaso A., Mizuno S., Nishimori K. J. Biochem. 117:1050-1057(1995) [PubMed: 8586618] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human DNA-(cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1." Chuang L.S.-H., Ian H.-I., Koh T.-W., Ng H.-H., Xu G., Li B.F.L. Science 277:1996-2000(1997) [PubMed: 9302295] [Abstract] Cited for: INTERACTION WITH PCNA, MUTAGENESIS OF VAL-190.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D43920 mRNA. Translation: BAA07867.1.
IPI	IPI00588424.
PIR	JC4172.
RefSeq	NP_996835.1.
UniGene	Gga.1206
3D structure databases
SMR	Q92072. Positions 557-607, 898-1010, 1054-1509.
ModBase	Search...
Protein family/group databases
REBASE	3020. M.GgaDnmt1.
Proteomic databases
PRIDE	Q92072.
Genome annotation databases
GeneID	396011.
KEGG	gga:396011.
Organism-specific databases
CTD	396011.
Phylogenomic databases
eggNOG	veNOG07492.
HOVERGEN	Q92072.
PhylomeDB	Q92072.
Enzyme and pathway databases
BRENDA	2.1.1.37. 4.
Family and domain databases
InterPro	IPR001025. BAH_dom. IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR010506. DMAP1_bd. IPR017198. DNA_C5-MeTrfase_1. IPR002857. Znf_CXXC. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF01426. BAH. 2 hits. PF06464. DMAP_binding. 1 hit. PF00145. DNA_methylase. 1 hit. PF02008. zf-CXXC. 1 hit. [Graphical view]
PIRSF	PIRSF037404. DNMT1. 1 hit.
PRINTS	PR00105. C5METTRFRASE.
SMART	SM00439. BAH. 2 hits. [Graphical view]
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS51038. BAH. 2 hits. PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. PS51058. ZF_CXXC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNMT1_CHICK

Accession

Primary (citable) accession number: Q92072

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)