Reviewed, UniProtKB/Swiss-Prot Q92058 (PPBT_CHICK)

Names and origin

Protein names

Recommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme
      Short name=AP-TNAP
      Short name=TNSALP
    EC=3.1.3.1

Gene names

Name:

ALPL

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 498	482	Alkaline phosphatase, tissue-nonspecific isozyme		PRO_0000024029
	Propeptide	499 – 519	21	Removed in mature form Potential		PRO_0000024030
Sites
	Active site	109	1	Phosphoserine intermediate By similarity
	Metal binding	59	1	Magnesium Potential
	Metal binding	59	1	Zinc 2 Potential
	Metal binding	172	1	Magnesium Potential
	Metal binding	331	1	Magnesium Potential
	Metal binding	336	1	Zinc 1 Potential
	Metal binding	340	1	Zinc 1 Potential
	Metal binding	377	1	Zinc 2 Potential
	Metal binding	378	1	Zinc 2 Potential
	Metal binding	453	1	Zinc 1 Potential
Amino acid modifications
	Lipidation	498	1	GPI-anchor amidated serine Potential
	Glycosylation	139	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
	Glycosylation	278	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q92058-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7934C0EEC3B17B89	FASTA	519	56,760
	10 20 30 40 50 60 MKAFLLTLLA QLCSASLVPE REKDPEYWRQ QAQETLRDAL RLQHLNQNVA KNLILFLGDG 70 80 90 100 110 120 MGVSTVTAAR ILKGQLQHRK GEESLLEMDK FPYVALAKTY NTNAQVPDSA GTATAYLCGV 130 140 150 160 170 180 KANEGTVGVS AGVTRDRCNT TKGQEVTSIL RWAKDEGKAV GIVTTTRVTH ATPSAAYAHS 190 200 210 220 230 240 ANRDWYSDGE MPLDALEGGC KDIARQLVDN IPDIEVILGG GRKYMFPKNT SDVEYPQEER 250 260 270 280 290 300 HRGTRLDGKD LVQAWHDTKP AGKVAKYVWH RRELLALNVS RVDFLLGLFE PGDMVYELDR 310 320 330 340 350 360 NNETDPSLSE MVAVAIRMLQ KNPRGFFLLV EGGRIDHGHH EGKAKQALHE AVELDRAVGL 370 380 390 400 410 420 AGRLTSPRDT LSVVTADHSH VFTFGGYTPR GNPIFGLAPM QSDVDRKPFT SILYGNGPGY 430 440 450 460 470 480 KIVGGERENV SAVDFAHANY QAQAAVPLRQ ETHGGEDVAV FARGPMAHLL HGVDEQNYIP 490 500 510 HAMAYAACIG PNRAHCSSAA RPAATATLLP VLLLLLLLC

References

[1]	"Tissue-nonspecific alkaline phosphatase participates in the establishment and growth of feather germs in embryonic chick skin cultures." Crawford K., Weissig H., Binette F., Millan J.L., Goetinck P.F. Dev. Dyn. 204:48-56(1995) [PubMed: 8563025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19108 mRNA. Translation: AAA92562.1.
IPI	IPI00590885.
UniGene	Gga.760
3D structure databases
SMR	Q92058. Positions 17-498.
ModBase	Search...
Protein-protein interaction databases
STRING	Q92058.
Proteomic databases
PRIDE	Q92058.
Genome annotation databases
Ensembl	ENSGALT00000039123; ENSGALP00000038334; ENSGALG00000023542; Gallus gallus. [Genome view]
Phylogenomic databases
eggNOG	veNOG11760.
HOGENOM	HBG617166.
HOVERGEN	Q92058.
InParanoid	Q92058.
PhylomeDB	Q92058.
Enzyme and pathway databases
BRENDA	3.1.3.1. 4.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. IPR017850. Alkaline_phosphatase_core. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPBT_CHICK

Accession

Primary (citable) accession number: Q92058

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)