Reviewed, UniProtKB/Swiss-Prot Q92055 (LDHA_FUNHE)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27
Alternative name(s):
    LDH-M

Gene names

Name:

ldha

Organism

Fundulus heteroclitus (Killifish) (Mummichog)

Taxonomic identifier

8078 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Cyprinodontiformes › Fundulidae › Fundulus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 332	331	L-lactate dehydrogenase A chain		PRO_0000168438
Regions
	Nucleotide binding	29 – 57	29	NAD By similarity
Sites
	Active site	193	1	Proton acceptor By similarity
	Binding site	99	1	NAD By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	138	1	NAD or substrate By similarity
	Binding site	169	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92055-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E518179F537C8D88	FASTA	332	36,334
	10 20 30 40 50 60 MSTQEKLISH VMKEEPVGCR NKVTVVGVGM VGMASAISVL LKDLCDELAL VDVMEDKLKG 70 80 90 100 110 120 EAMDLQHGAL FLKTHKIVAD KDYSVTANSK VVVVTAGARQ QEGESRLNLV QRNVNIFKFI 130 140 150 160 170 180 IPNIVKYSPN CILLVVSNPV DILTYVAWKL SGFPRHRVIG SGTNLDSARF RHLMGEKFHL 190 200 210 220 230 240 HPSSCHGWIV GEHGDSSVAV WSGVNIAGVS LQTLNPNMGA DGDSENWKEL HKKVVDGAYE 250 260 270 280 290 300 VIKLKGYTSW AIGMSVADLV ESIVKNLHKV HPVSTLVQGM HGVKDEVFLS IPCVLGNSGL 310 320 330 TDVIHMTLKP EEEKQLVKSA ETLWGVQKEL TL

References

[1]	"Evolutionary relations among vertebrate muscle-type lactate dehydrogenases." Quattro J.M., Pollock D.D., Powell M., Woods H.A., Powers D.A. Mol. Mar. Biol. Biotechnol. 4:224-231(1995) [PubMed: 7670598] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43525 mRNA. Translation: AAA99462.1.
3D structure databases
HSSP	HSSP built from PDB template 5LDH based on UniProtKB P00336.
SMR	Q92055. Positions 2-332.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q92055.
Enzyme and pathway databases
BRENDA	1.1.1.27. 39477.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHA_FUNHE

Accession

Primary (citable) accession number: Q92055

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)