Q92047 (PIMT_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase
Short name=PIMT
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase

Gene names

Name:

pcmt

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 228	227	Protein-L-isoaspartate(D-aspartate) O-methyltransferase		PRO_0000111879
Sites
	Active site	60	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92047 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0266456304972562	FASTA	228	24,725
	10 20 30 40 50 60 MAWKSGGASH AELVNNLRKN GIIKSDRVYE VMLATDRSHF SRCNPYMDSP QSIGYQATIS 70 80 90 100 110 120 APHMHAYALE LLHDHLYEGA KALDVGSGSG ILSVCFSRMV GPTGKVIGID HIKELVEDSI 130 140 150 160 170 180 ANVKKDDPSL ITSGRIKLIV GDGRMGFTEE APYDAIHVGA AAPTVPQALL DQLKPGGRLI 190 200 210 220 LPVGPAGGNQ MLEQYDKLED GSTKMKPLMG VIYVPLTDKD KQWSRDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular phylogenetics of a protein repair methyltransferase." Kagan R.M., McFadden H.J., McFadden P.N., O'Connor C., Clarke S. Comp. Biochem. Physiol. 117B:379-385(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37434 mRNA. Translation: AAA96020.1. BC075735 mRNA. Translation: AAH75735.1.
IPI	IPI00510557.
RefSeq	NP_571540.1. NM_131465.2.
UniGene	Dr.372.
3D structure databases
ProteinModelPortal	Q92047.
SMR	Q92047. Positions 3-225.
ModBase	Search...
Proteomic databases
PRIDE	Q92047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000092718; ENSDARP00000087150; ENSDARG00000015201.
GeneID	30751.
KEGG	dre:30751.
Organism-specific databases
CTD	30751.
ZFIN	ZDB-GENE-990415-134. pcmt.
Phylogenomic databases
eggNOG	COG2518.
GeneTree	ENSGT00510000046974.
HOVERGEN	HBG004483.
InParanoid	Q92047.
KO	K00573.
OrthoDB	EOG4NGGNK.
Gene expression databases
ArrayExpress	Q92047.
Bgee	Q92047.
Family and domain databases
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20807097.

Entry information

Entry name

PIMT_DANRE

Accession

Primary (citable) accession number: Q92047

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program