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Protein

Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene

pcmt

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601By similarity

GO - Molecular functioni

  1. protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (EC:2.1.1.77)
Short name:
PIMT
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Gene namesi
Name:pcmt
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-990415-134. pcmt.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 228227Protein-L-isoaspartate(D-aspartate) O-methyltransferasePRO_0000111879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ92047.

Expressioni

Gene expression databases

BgeeiQ92047.
ExpressionAtlasiQ92047. differential.

Structurei

3D structure databases

ProteinModelPortaliQ92047.
SMRiQ92047. Positions 3-225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2518.
GeneTreeiENSGT00510000046974.
HOVERGENiHBG004483.
InParanoidiQ92047.
KOiK00573.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
PfamiPF01135. PCMT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWKSGGASH AELVNNLRKN GIIKSDRVYE VMLATDRSHF SRCNPYMDSP
60 70 80 90 100
QSIGYQATIS APHMHAYALE LLHDHLYEGA KALDVGSGSG ILSVCFSRMV
110 120 130 140 150
GPTGKVIGID HIKELVEDSI ANVKKDDPSL ITSGRIKLIV GDGRMGFTEE
160 170 180 190 200
APYDAIHVGA AAPTVPQALL DQLKPGGRLI LPVGPAGGNQ MLEQYDKLED
210 220
GSTKMKPLMG VIYVPLTDKD KQWSRDEL
Length:228
Mass (Da):24,725
Last modified:January 23, 2007 - v3
Checksum:i0266456304972562
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37434 mRNA. Translation: AAA96020.1.
BC075735 mRNA. Translation: AAH75735.1.
RefSeqiNP_571540.1. NM_131465.2.
UniGeneiDr.372.

Genome annotation databases

EnsembliENSDART00000092718; ENSDARP00000087150; ENSDARG00000015201.
GeneIDi30751.
KEGGidre:30751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37434 mRNA. Translation: AAA96020.1.
BC075735 mRNA. Translation: AAH75735.1.
RefSeqiNP_571540.1. NM_131465.2.
UniGeneiDr.372.

3D structure databases

ProteinModelPortaliQ92047.
SMRiQ92047. Positions 3-225.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ92047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000092718; ENSDARP00000087150; ENSDARG00000015201.
GeneIDi30751.
KEGGidre:30751.

Organism-specific databases

CTDi30751.
ZFINiZDB-GENE-990415-134. pcmt.

Phylogenomic databases

eggNOGiCOG2518.
GeneTreeiENSGT00510000046974.
HOVERGENiHBG004483.
InParanoidiQ92047.
KOiK00573.

Miscellaneous databases

NextBioi20807097.
PROiQ92047.

Gene expression databases

BgeeiQ92047.
ExpressionAtlasiQ92047. differential.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
PfamiPF01135. PCMT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiPIMT_DANRE
AccessioniPrimary (citable) accession number: Q92047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.