Reviewed, UniProtKB/Swiss-Prot Q92045 (CP11A_DASAM)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 11A1, mitochondrial
    EC=1.14.15.6
Alternative name(s):
    CYPXIA1
    Cholesterol side-chain cleavage enzyme
    P450(SCC)
    Cholesterol desmolase

Gene names

Name:

CYP11A1

Organism

Dasyatis americana (Southern stingray)

Taxonomic identifier

50411 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Squalea › Hypnosqualea › Pristiorajea › Batoidea › Myliobatiformes › Myliobatoidei › Dasyatidae › Dasyatis

Protein attributes

Sequence length	514 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Catalytic activity	Cholesterol + reduced adrenal ferredoxin + O(2) = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H(2)O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol monooxygenase (side-chain-cleaving) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 39	›39	Mitochondrion By similarity
	Chain	40 – 514	475	Cytochrome P450 11A1, mitochondrial		PRO_0000003592
Sites
	Metal binding	461	1	Iron (heme axial ligand) By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q92045-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D0F03E25D5534FB2	FASTA	514	59,863
	10 20 30 40 50 60 SFRLSLSAST YAQRGSFTTP EHDFTLFPHR NHSVTSESRI PSEQTLKSLT DIPGNWRKNW 70 80 90 100 110 120 LNVYYFWRSN GLNNAHQWML DNFNKYGPIY REKIAYYESI NIINPADAVI MNKSEGPFPK 130 140 150 160 170 180 RIEMAPWVAY RDLRKENYGV QLLNGENWKR TRLILNNSIF AQSSIQRLVP LFNEVVLDFV 190 200 210 220 230 240 SMVHKEVEKS RSDYWKTDLT NDLFKLALEV ICYILYGERL DLLQRKYNKA PQKFIDSIAT 250 260 270 280 290 300 MFHSTPIMLY VPPSLLKSIN SKIWQQHVGS WDNIFEHADT YLKKAYRQFQ QGSKNEHAFP 310 320 330 340 350 360 GVLTELLLQG ALPFEDIRAS IIDVMSGAID TTSTTVHWMM YELAKHPHIQ KNVRSEIMEA 370 380 390 400 410 420 HQKTEGDPVK MLKSVPLLKC VVKETLRLYP VAISIQRYLN EDTVLQNYHI PAGTLVQLGL 430 440 450 460 470 480 YAMGRNPKIF KNPEQYNPER WLKGEDTHFR HLGFGFGPRQ CIGRRIAETQ MVLLMIHMLQ 490 500 510 NFKIETDPMT EVKSKFSLIL IPDKPINLKF TPIK

References

[1]	"Isolation of the putative cDNA encoding cholesterol side chain cleavage cytochrome P450 (CYP11A) of the southern stingray (Dasyatis americana)." Nunez S., Trant J.M. Gene 187:123-129(1997) [PubMed: 9073075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Interrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U63299 mRNA. Translation: AAC60095.1.
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q92045.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP11A_DASAM

Accession

Primary (citable) accession number: Q92045

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)