Reviewed, UniProtKB/Swiss-Prot Q92038 (ACOD_CYPCA)

Names and origin

Protein names	Recommended name:     Acyl-CoA desaturase     EC=1.14.19.1 Alternative name(s):     Stearoyl-CoA desaturase     Fatty acid desaturase     Delta(9)-desaturase
Organism	Cyprinus carpio (Common carp)
Taxonomic identifier	7962 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Cyprinus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O2 and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA By similarity.
Catalytic activity	Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.
Cofactor	Iron By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Induction	By cold. A 10-fold increase in transcript levels is observed 48-60 hours after cooling. Ref.1
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the fatty acid desaturase family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Stress response
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Iron
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW stearoyl-CoA 9-desaturase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Acyl-CoA desaturase		PRO_0000185403
Regions
	Transmembrane	43 – 63	21	Potential
	Transmembrane	65 – 85	21	Potential
	Transmembrane	199 – 219	21	Potential
	Motif	87 – 92	6	Histidine box-1
	Motif	124 – 128	5	Histidine box-2
	Motif	265 – 269	5	Histidine box-3

Sequences

	Sequence		Length	Mass (Da)
	Q92038-1 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: 3E794CB408D33089	FASTA	327	37,820
	10 20 30 40 50 60 MPDREIKSPI WHPEPGTVED VFDHTYKEKE GPKPPTVIVW RNVILMSLLH LGALYGLFLF 70 80 90 100 110 120 PSARALTWIW FFGCLLFSAL GITAGAHRLW SHRSYKASLP LQIFLALGNS MAFQNDIYEW 130 140 150 160 170 180 SRDHRVHHKY SETDADPHNA VRGFFFSHVG WLLVRKHPDV IEKGRKLELS DLKADKVVMF 190 200 210 220 230 240 QRRFYKPSVL LMCFFVPTFV PWYVWGESLW VAYFVPALLR YALVLNATWL VNSAAHMWGN 250 260 270 280 290 300 RPYDSSINPR ENRFVTFSAI GEGFHNYHHT FPFDYATSEF GCKLNLTTCC FIDLMCFLGL 310 320 AREPKRVSRE AVLARAQRTG DGSHWSG

References

[1]	"Cold-induced expression of delta 9-desaturase in carp by transcriptional and posttranslational mechanisms." Tiku P.E., Gracey A.Y., Macartney A.I., Beynon R.J., Cossins A.R. Science 271:815-818(1996) [PubMed: 8629000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Tissue: Liver.
[2]	Tiku P.E. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 11-25 AND C-TERMINUS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31864 mRNA. Translation: AAB03857.2.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q92038.
Enzyme and pathway databases
BRENDA	1.14.19.1. 3298.
Family and domain databases
InterPro	IPR005804. Fatty_acid_desaturase-1. IPR001522. Fatty_acid_desaturase-1_C. IPR015876. Fatty_acid_desaturase-1_core. [Graphical view]
Pfam	PF00487. FA_desaturase. 1 hit. [Graphical view]
PRINTS	PR00075. FACDDSATRASE.
ProDom	PD002221. Desaturase. 1 hit. PD001081. FA_desat_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00476. FATTY_ACID_DESATUR_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACOD_CYPCA

Accession

Primary (citable) accession number: Q92038

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)