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Q92037 (NOS2_CARAU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:nos2
OrganismCarassius auratus (Goldfish)
Taxonomic identifier7957 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

Protein attributes

Sequence length164 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Not stimulated by calcium/calmodulin By similarity.

Subunit structure

Homodimer By similarity.

Induction

By lipopolysaccharide (LPS).

Sequence similarities

Belongs to the NOS family.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›164›164Nitric oxide synthase, inducible
PRO_0000170940

Regions

Domain66 – ›164›99Flavodoxin-like
Nucleotide binding150 – ›164›15FMN By similarity
Region36 – 5621Calmodulin-binding Potential

Experimental info

Non-terminal residue11
Non-terminal residue1641

Sequences

Sequence LengthMass (Da)Tools
Q92037 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CD584B9D47A285F4

FASTA16419,045
        10         20         30         40         50         60 
FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL FSQTLIKSAL 

        70         80         90        100        110        120 
AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME DYNFSELEKE SLLFVVTSTF 

       130        140        150        160 
GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV FGLGSRMYPH FCAF 

« Hide

References

[1]"A partial sequence for nitric oxide synthase from a goldfish (Carassius auratus) macrophage cell line."
Laing K.J., Grawbowski P.S., Belosevic M., Secombes C.J.
Immunol. Cell Biol. 74:374-379(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Macrophage.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97603 mRNA. Translation: CAA66203.1.

3D structure databases

ProteinModelPortalQ92037.
SMRQ92037. Positions 3-31.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.360. 1 hit.
InterProIPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR004030. NO_synthase_oxygenase_dom.
[Graphical view]
PfamPF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PRINTSPR00369. FLAVODOXIN.
SUPFAMSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
PROSITEPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS2_CARAU
AccessionPrimary (citable) accession number: Q92037
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families