Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitric oxide synthase, inducible

Gene

nos2

Organism
Carassius auratus (Goldfish)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity
  • 5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity

Enzyme regulationi

Not stimulated by calcium/calmodulin.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – ›164›15FMNPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:nos2
OrganismiCarassius auratus (Goldfish)
Taxonomic identifieri7957 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›164›164Nitric oxide synthase, induciblePRO_0000170940Add
BLAST

Expressioni

Inductioni

By lipopolysaccharide (LPS).

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ92037.
SMRiQ92037. Positions 3-31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – ›164›99Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 5621Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR004030. NOS_N.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q92037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL
60 70 80 90 100
FSQTLIKSAL AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME
110 120 130 140 150
DYNFSELEKE SLLFVVTSTF GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV
160
FGLGSRMYPH FCAF
Length:164
Mass (Da):19,045
Last modified:November 1, 1996 - v1
Checksum:iCD584B9D47A285F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei164 – 1641

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97603 mRNA. Translation: CAA66203.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97603 mRNA. Translation: CAA66203.1.

3D structure databases

ProteinModelPortaliQ92037.
SMRiQ92037. Positions 3-31.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR004030. NOS_N.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A partial sequence for nitric oxide synthase from a goldfish (Carassius auratus) macrophage cell line."
    Laing K.J., Grawbowski P.S., Belosevic M., Secombes C.J.
    Immunol. Cell Biol. 74:374-379(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Macrophage.

Entry informationi

Entry nameiNOS2_CARAU
AccessioniPrimary (citable) accession number: Q92037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.