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Protein

Nitric oxide synthase, inducible

Gene

nos2

Organism
Carassius auratus (Goldfish)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Not stimulated by calcium/calmodulin.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi150 – ›164FMNPROSITE-ProRule annotationAdd BLAST›15

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Oxidoreductase
LigandFAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:nos2
OrganismiCarassius auratus (Goldfish)
Taxonomic identifieri7957 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000170940‹1 – ›164Nitric oxide synthase, inducibleAdd BLAST›164

Expressioni

Inductioni

By lipopolysaccharide (LPS).

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ92037.
SMRiQ92037.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – ›164Flavodoxin-likePROSITE-ProRule annotationAdd BLAST›99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 56Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the NOS family.Curated

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiView protein in InterPro
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like_dom.
IPR036119. NOS_N_sf.
PfamiView protein in Pfam
PF00258. Flavodoxin_1. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
PROSITEiView protein in PROSITE
PS50902. FLAVODOXIN_LIKE. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q92037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL
60 70 80 90 100
FSQTLIKSAL AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME
110 120 130 140 150
DYNFSELEKE SLLFVVTSTF GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV
160
FGLGSRMYPH FCAF
Length:164
Mass (Da):19,045
Last modified:November 1, 1996 - v1
Checksum:iCD584B9D47A285F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-terminal residuei1641

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97603 mRNA. Translation: CAA66203.1.

Similar proteinsi

Entry informationi

Entry nameiNOS2_CARAU
AccessioniPrimary (citable) accession number: Q92037
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families