Reviewed, UniProtKB/Swiss-Prot Q92037 (NOS2_CARAU)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase, inducible
    EC=1.14.13.39
Alternative name(s):
    Inducible NO synthase
      Short name=Inducible NOS
      Short name=iNOS
    NOS type II

Gene names

Name:

nos2

Organism

Carassius auratus (Goldfish)

Taxonomic identifier

7957 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Carassius

Protein attributes

Sequence length	164 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Not stimulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer By similarity.
Induction	By lipopolysaccharide (LPS).
Sequence similarities	Belongs to the NOS family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›164	›164	Nitric oxide synthase, inducible		PRO_0000170940
Regions
	Domain	66 – ›164	›99	Flavodoxin-like
	Nucleotide binding	150 – ›164	›15	FMN By similarity
	Region	36 – 56	21	Calmodulin-binding Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	164	1

Sequences

	Sequence		Length	Mass (Da)
	Q92037-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CD584B9D47A285F4	FASTA	164	19,045
	10 20 30 40 50 60 FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL FSQTLIKSAL 70 80 90 100 110 120 AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME DYNFSELEKE SLLFVVTSTF 130 140 150 160 GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV FGLGSRMYPH FCAF

References

[1]	"A partial sequence for nitric oxide synthase from a goldfish (Carassius auratus) macrophage cell line." Laing K.J., Grawbowski P.S., Belosevic M., Secombes C.J. Immunol. Cell Biol. 74:374-379(1996) [PubMed: 8872189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97603 mRNA. Translation: CAA66203.1.
3D structure databases
HSSP	HSSP built from PDB template 1D0C based on UniProtKB P29473.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q92037.
Family and domain databases
InterPro	IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR004030. NO_synthase_oxygenase_reg. [Graphical view]
Gene3D	G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.
Pfam	PF00258. Flavodoxin_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN.
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_CARAU

Accession

Primary (citable) accession number: Q92037

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)