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Q92037

- NOS2_CARAU

UniProt

Q92037 - NOS2_CARAU

Protein

Nitric oxide synthase, inducible

Gene

nos2

Organism
Carassius auratus (Goldfish)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity
    Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.By similarity

    Enzyme regulationi

    Not stimulated by calcium/calmodulin.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi150 – ›164›15FMNPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro
    2. peptidyl-cysteine S-nitrosylation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, inducible (EC:1.14.13.39)
    Alternative name(s):
    Inducible NO synthase
    Short name:
    Inducible NOS
    Short name:
    iNOS
    NOS type II
    Peptidyl-cysteine S-nitrosylase NOS2
    Gene namesi
    Name:nos2
    OrganismiCarassius auratus (Goldfish)
    Taxonomic identifieri7957 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›164›164Nitric oxide synthase, induciblePRO_0000170940Add
    BLAST

    Expressioni

    Inductioni

    By lipopolysaccharide (LPS).

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ92037.
    SMRiQ92037. Positions 3-31.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – ›164›99Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 5621Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Curated
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.360. 1 hit.
    InterProiIPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR029039. Flavoprotein-like.
    IPR004030. NOS_N.
    [Graphical view]
    PfamiPF00258. Flavodoxin_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PRINTSiPR00369. FLAVODOXIN.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q92037-1 [UniParc]FASTAAdd to Basket

    « Hide

    FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL    50
    FSQTLIKSAL AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME 100
    DYNFSELEKE SLLFVVTSTF GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV 150
    FGLGSRMYPH FCAF 164
    Length:164
    Mass (Da):19,045
    Last modified:November 1, 1996 - v1
    Checksum:iCD584B9D47A285F4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei164 – 1641

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97603 mRNA. Translation: CAA66203.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97603 mRNA. Translation: CAA66203.1 .

    3D structure databases

    ProteinModelPortali Q92037.
    SMRi Q92037. Positions 3-31.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.360. 1 hit.
    InterProi IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR029039. Flavoprotein-like.
    IPR004030. NOS_N.
    [Graphical view ]
    Pfami PF00258. Flavodoxin_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PRINTSi PR00369. FLAVODOXIN.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    PROSITEi PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A partial sequence for nitric oxide synthase from a goldfish (Carassius auratus) macrophage cell line."
      Laing K.J., Grawbowski P.S., Belosevic M., Secombes C.J.
      Immunol. Cell Biol. 74:374-379(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Macrophage.

    Entry informationi

    Entry nameiNOS2_CARAU
    AccessioniPrimary (citable) accession number: Q92037
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3