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Q92037

- NOS2_CARAU

UniProt

Q92037 - NOS2_CARAU

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Protein

Nitric oxide synthase, inducible

Gene
nos2
Organism
Carassius auratus (Goldfish)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulationi

Not stimulated by calcium/calmodulin By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – ›164›15FMN By similarityAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:nos2
OrganismiCarassius auratus (Goldfish)
Taxonomic identifieri7957 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›164›164Nitric oxide synthase, induciblePRO_0000170940Add
BLAST

Expressioni

Inductioni

By lipopolysaccharide (LPS).

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ92037.
SMRiQ92037. Positions 3-31.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – ›164›99Flavodoxin-likeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 5621Calmodulin-binding Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR004030. NOS_N.
[Graphical view]
PfamiPF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PRINTSiPR00369. FLAVODOXIN.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
PROSITEiPS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q92037-1 [UniParc]FASTAAdd to Basket

« Hide

FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL    50
FSQTLIKSAL AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME 100
DYNFSELEKE SLLFVVTSTF GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV 150
FGLGSRMYPH FCAF 164
Length:164
Mass (Da):19,045
Last modified:November 1, 1996 - v1
Checksum:iCD584B9D47A285F4
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei164 – 1641

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97603 mRNA. Translation: CAA66203.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X97603 mRNA. Translation: CAA66203.1 .

3D structure databases

ProteinModelPortali Q92037.
SMRi Q92037. Positions 3-31.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.360. 1 hit.
InterProi IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR029039. Flavoprotein-like.
IPR004030. NOS_N.
[Graphical view ]
Pfami PF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PRINTSi PR00369. FLAVODOXIN.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
PROSITEi PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A partial sequence for nitric oxide synthase from a goldfish (Carassius auratus) macrophage cell line."
    Laing K.J., Grawbowski P.S., Belosevic M., Secombes C.J.
    Immunol. Cell Biol. 74:374-379(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Macrophage.

Entry informationi

Entry nameiNOS2_CARAU
AccessioniPrimary (citable) accession number: Q92037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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