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Reviewed, UniProtKB/Swiss-Prot Q92037 (NOS2_CARAU)

Last modified November 25, 2008. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Nitric oxide synthase, inducible
    EC=1.14.13.39
Alternative name(s):
    Inducible NO synthase
      Short name=Inducible NOS
      Short name=iNOS
    NOS type II
Gene names
Name: nos2
OrganismCarassius auratus (Goldfish)
Taxonomic identifier7957 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

Protein attributes

Sequence length164 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Catalytic activity

L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Not stimulated by calcium/calmodulin By similarity.

Subunit structure

Homodimer By similarity.

Induction

By lipopolysaccharide (LPS).

Sequence similarities

Belongs to the NOS family.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›164›164Nitric oxide synthase, inducible
PRO_0000170940

Regions

Domain66 – ›164›99Flavodoxin-like
Nucleotide binding150 – ›164›15FMN By similarity
Region36 – 5621Calmodulin-binding Potential

Experimental info

Non-terminal residue11
Non-terminal residue1641

Sequences

Sequence LengthMass (Da)Tools
Q92037-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CD584B9D47A285F4

FASTA16419,045
        10         20         30         40         50         60 
FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL FSQTLIKSAL 

        70         80         90        100        110        120 
AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME DYNFSELEKE SLLFVVTSTF 

       130        140        150        160 
GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV FGLGSRMYPH FCAF 

« Hide

References

[1]"A partial sequence for nitric oxide synthase from a goldfish (Carassius auratus) macrophage cell line."
Laing K.J., Grawbowski P.S., Belosevic M., Secombes C.J.
Immunol. Cell Biol. 74:374-379(1996) [PubMed: 8872189] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Macrophage.

Cross-references

Sequence databases

X97603 mRNA. Translation: CAA66203.1.

3D structure databases

HSSPHSSP built from PDB template 1D0C based on UniProtKB P29473.
ModBaseSearch...

Phylogenomic databases

HOVERGENQ92037.

Family and domain databases

InterProIPR001094. Flavdoxin_like.
IPR008254. Flavodoxin/NO_synth.
IPR004030. NO_synthase_oxygenase_reg.
[Graphical view]
Gene3DG3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.
PfamPF00258. Flavodoxin_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PRINTSPR00369. FLAVODOXIN.
PROSITEPS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. Partial match.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS2_CARAU
AccessionPrimary (citable) accession number: Q92037
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents