Q92037 (NOS2_CARAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitric oxide synthase, inducible
EC=1.14.13.39

Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2

Gene names

Name:

nos2

Organism

Carassius auratus (Goldfish)

Taxonomic identifier

7957 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Carassius

Protein attributes

Sequence length	164 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.
Catalytic activity	L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Not stimulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer By similarity.
Induction	By lipopolysaccharide (LPS).
Sequence similarities	Belongs to the NOS family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro peptidyl-cysteine S-nitrosylation Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›164	›164	Nitric oxide synthase, inducible		PRO_0000170940
Regions
	Domain	66 – ›164	›99	Flavodoxin-like
	Nucleotide binding	150 – ›164	›15	FMN By similarity
	Region	36 – 56	21	Calmodulin-binding Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	164	1

Sequences

	Sequence		Length	Mass (Da)
	Q92037 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CD584B9D47A285F4	FASTA	164	19,045
	10 20 30 40 50 60 FGFHQQMLNY ILSPFFYYQP DPWLTHKWKD EKRNMRKHSI SFKGLIRAVL FSQTLIKSAL 70 80 90 100 110 120 AKRVRCTVLY ATETGKSKTL AKKLNTMMNY AFSSKVVCME DYNFSELEKE SLLFVVTSTF 130 140 150 160 GNGDCPGNGE SFKKQLLSLN NLRNQVRYSV FGLGSRMYPH FCAF

References

[1]	"A partial sequence for nitric oxide synthase from a goldfish (Carassius auratus) macrophage cell line." Laing K.J., Grawbowski P.S., Belosevic M., Secombes C.J. Immunol. Cell Biol. 74:374-379(1996) [PubMed: 8872189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97603 mRNA. Translation: CAA66203.1.
3D structure databases
ProteinModelPortal	Q92037.
SMR	Q92037. Positions 3-31.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR012144. Nitric-oxide_synthase. IPR004030. NO_synthase_oxygenase_dom. [Graphical view]
PANTHER	PTHR19384:SF5. PTHR19384:SF5. 1 hit.
Pfam	PF00258. Flavodoxin_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN.
SUPFAM	SSF56512. NO_synthase_oxygenase_reg. 1 hit.
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_CARAU

Accession

Primary (citable) accession number: Q92037

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program