Sentrin-specific protease 2 - Mus musculus (Mouse)

Names and origin

Protein names

Recommended name:
    Sentrin-specific protease 2
    EC=3.4.22.-
Alternative name(s):
    Sentrin/SUMO-specific protease SENP2
    SUMO-1/Smt3-specific isopeptidase 2
      Short name=Smt3ip2
    Axam2
    SUMO-1 protease 1
      Short name=SuPr-1

Gene names

Name:	Senp2
Synonyms:	Smt3ip2, Supr1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway By similarity. Isoform 3 activates transcription. Ref.1 Ref.2
Subunit structure	Binds to SUMO2 and SUMO3. Interacts with the C-terminal domain of NUP153 via its N-terminus By similarity.
Subcellular location	Nucleus Ref.2. Isoform 1: Nucleus › nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side By similarity Ref.2. Isoform 2: Cytoplasm. Cytoplasmic vesicle. Note: Found in the cytoplasm and in cytoplasmic vesicles, together with axin. Ref.2 Isoform 3: Nucleus. Note: Found in the PML nuclear bodies. Ref.2
Tissue specificity	Highly expressed in testis. Detected in brain, heart and thymus. Ref.2
Domain	The N-terminus is necessary and sufficient for nuclear envelope targeting By similarity.
Post-translational modification	Polyubiquitinated; which leads to proteasomal degradation By similarity.
Sequence similarities	Belongs to the peptidase C48 family.

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Ontologies

Keywords
Biological process	Protein transport Transcription Transcription regulation Translocation Transport Ubl conjugation pathway Wnt signaling pathway mRNA transport
Cellular component	Cytoplasm Cytoplasmic vesicle Membrane Nuclear pore complex Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Activator Hydrolase Protease Thiol protease
PTM	Phosphoprotein Ubl conjugation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein desumoylation Inferred from direct assay. Source: MGI protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear pore Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	SUMO-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91ZX6-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91ZX6-2) The sequence of this isoform differs from the canonical sequence as follows: 1-53: MYRWLAKVLGTILRLCERPAPGARALLKRRRSSSTLFSTAVDTDEIPAKRPRL → MEQNSK
Note: Found in the cytoplasm and in cytoplasmic vesicles, together with axin. Ref.2 No experimental confirmation available.

Isoform 3 (identifier: Q91ZX6-3) The sequence of this isoform differs from the canonical sequence as follows: 1-81: Missing.
Note: Found in the PML nuclear bodies. Ref.2

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 588

588

Sentrin-specific protease 2

PRO_0000101719

Regions

Region

394 – 558

165

Protease

Motif

28 – 31

4

Nuclear localization signal Potential

Motif

47 – 52

6

Nuclear localization signal Potential

Motif

316 – 331

16

Nuclear export signal By similarity

Sites

Active site

477

1

By similarity

Active site

494

1

By similarity

Active site

547

1

Nucleophile

Amino acid modifications

Modified residue

217

1

Phosphotyrosine By similarity

Modified residue

332

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 81

81

Missing in isoform 3.

VSP_021942

Alternative sequence

1 – 53

53

MYRWL…KRPRL → MEQNSK in isoform 2.

VSP_005273

Experimental info

Mutagenesis

547

1

C → A: Abolishes protease activity. Ref.1 Ref.2

Sequence conflict

220

1

L → F in AAL14437. Ref.1

Sequence conflict

255

1

W → C in AAL14437. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified November 28, 2002. Version 2. Checksum: 09B56796CA194847 Show »	FASTA	588	67,579
10 20 30 40 50 60 MYRWLAKVLG TILRLCERPA PGARALLKRR RSSSTLFSTA VDTDEIPAKR PRLDCFIHQV 70 80 90 100 110 120 KNSLYNAASL FGFPFQLTTK PMVSSACNGT RNVAPSGEVF SNSSSCELMS SGSCSSMLKL 130 140 150 160 170 180 GNKSPNGISD YPKIRVTVTR DQPRRVLPSF GFTLKSEGYN RRPSGRRHSK SNPESSLTWK 190 200 210 220 230 240 PQEQGVTEMI SEEGGKGVRR PHCTVEEGVQ KDEREKYRKL LERLKEGAHG STFPPTVSHH 250 260 270 280 290 300 SSQRIQMDTL KTKGWVEEQN HGVRTTHFVP KQYRVVETRG PLCSMRSEKR YSKGKADTEK 310 320 330 340 350 360 VVGLRFEKEG TRGHQMEPDL SEEVSARLRL GSGSNGLLRR KISVLEIKEK NFPSKEKDRR 370 380 390 400 410 420 TEDLFEFTED MEKEISNALG HGPPDEILSS AFKLRITRGD IQTLKNYHWL NDEVINFYMN 430 440 450 460 470 480 LLVERSKKQG YPALHAFSTF FYPKLKSGGY QAVKRWTKGV NLFEQELVLV PIHRKVHWSL 490 500 510 520 530 540 VVMDLRKKCL KYLDSMGQKG HRICEILLQY LQDESKTKRN TDLNLLEWTH YSMKPHEIPQ 550 560 570 580 QLNGSDCGMF TCKYADYISR DKPITFTQHQ MPLFRKKMVW EILHQQLL « Hide
Isoform 2. Checksum: F25085A3FE0E0AE9 Show »	FASTA	541	62,276
10 20 30 40 50 60 MEQNSKDCFI HQVKNSLYNA ASLFGFPFQL TTKPMVSSAC NGTRNVAPSG EVFSNSSSCE 70 80 90 100 110 120 LMSSGSCSSM LKLGNKSPNG ISDYPKIRVT VTRDQPRRVL PSFGFTLKSE GYNRRPSGRR 130 140 150 160 170 180 HSKSNPESSL TWKPQEQGVT EMISEEGGKG VRRPHCTVEE GVQKDEREKY RKLLERLKEG 190 200 210 220 230 240 AHGSTFPPTV SHHSSQRIQM DTLKTKGWVE EQNHGVRTTH FVPKQYRVVE TRGPLCSMRS 250 260 270 280 290 300 EKRYSKGKAD TEKVVGLRFE KEGTRGHQME PDLSEEVSAR LRLGSGSNGL LRRKISVLEI 310 320 330 340 350 360 KEKNFPSKEK DRRTEDLFEF TEDMEKEISN ALGHGPPDEI LSSAFKLRIT RGDIQTLKNY 370 380 390 400 410 420 HWLNDEVINF YMNLLVERSK KQGYPALHAF STFFYPKLKS GGYQAVKRWT KGVNLFEQEL 430 440 450 460 470 480 VLVPIHRKVH WSLVVMDLRK KCLKYLDSMG QKGHRICEIL LQYLQDESKT KRNTDLNLLE 490 500 510 520 530 540 WTHYSMKPHE IPQQLNGSDC GMFTCKYADY ISRDKPITFT QHQMPLFRKK MVWEILHQQL L « Hide
Isoform 3. Checksum: CB6F154A1BABF39A Show »	FASTA	507	58,389
10 20 30 40 50 60 MVSSACNGTR NVAPSGEVFS NSSSCELMSS GSCSSMLKLG NKSPNGISDY PKIRVTVTRD 70 80 90 100 110 120 QPRRVLPSFG FTLKSEGYNR RPSGRRHSKS NPESSLTWKP QEQGVTEMIS EEGGKGVRRP 130 140 150 160 170 180 HCTVEEGVQK DEREKYRKLL ERLKEGAHGS TFPPTVSHHS SQRIQMDTLK TKGWVEEQNH 190 200 210 220 230 240 GVRTTHFVPK QYRVVETRGP LCSMRSEKRY SKGKADTEKV VGLRFEKEGT RGHQMEPDLS 250 260 270 280 290 300 EEVSARLRLG SGSNGLLRRK ISVLEIKEKN FPSKEKDRRT EDLFEFTEDM EKEISNALGH 310 320 330 340 350 360 GPPDEILSSA FKLRITRGDI QTLKNYHWLN DEVINFYMNL LVERSKKQGY PALHAFSTFF 370 380 390 400 410 420 YPKLKSGGYQ AVKRWTKGVN LFEQELVLVP IHRKVHWSLV VMDLRKKCLK YLDSMGQKGH 430 440 450 460 470 480 RICEILLQYL QDESKTKRNT DLNLLEWTHY SMKPHEIPQQ LNGSDCGMFT CKYADYISRD 490 500 KPITFTQHQM PLFRKKMVWE ILHQQLL « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel mammalian SUMO-1/Smt3-specific isopeptidase, a homologue of rat Axam, which is an Axin-binding protein promoting beta-Catenin degradation." Nishida T., Kaneko F., Kitagawa M., Yasuda H. J. Biol. Chem. 276:39060-39066(2001) [PubMed: 11489887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, MUTAGENESIS OF CYS-547. Tissue: Osteoblast.
[2]	"SUMO-1 protease-1 regulates gene transcription through PML." Best J.L., Ganiatsas S., Agarwal S., Changou A., Salomoni P., Shirihai O., Meluh P.B., Pandolfi P.P., Zon L.I. Mol. Cell 10:843-855(2002) [PubMed: 12419228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), MUTAGENESIS OF CYS-547, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis and Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary tumor.
[5]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 342-348 AND 489-499, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF368904 mRNA. Translation: AAL14437.1. AY188288 mRNA. Translation: AAO27902.1. AK015987 mRNA. Translation: BAB30067.1. AK031030 mRNA. Translation: BAC27222.1. BC031652 mRNA. Translation: AAH31652.1.
IPI	IPI00138091. IPI00230049. IPI00817049.
RefSeq	NP_083733.1.
UniGene	Mm.297431
3D structure databases
SMR	Q91ZX6. Positions 363-588.
ModBase	Search...
Protein-protein interaction databases
STRING	Q91ZX6.
Protein family/group databases
MEROPS	C48.007.
PTM databases
PhosphoSite	Q91ZX6.
Proteomic databases
PRIDE	Q91ZX6.
Genome annotation databases
Ensembl	ENSMUST00000023561; ENSMUSP00000023561; ENSMUSG00000022855; Mus musculus. [Genome view] ENSMUST00000115380; ENSMUSP00000111038; ENSMUSG00000022855; Mus musculus. [Genome view]
GeneID	75826.
KEGG	mmu:75826.
UCSC	uc007yrw.1. mouse. uc007yrx.1. mouse.
Organism-specific databases
CTD	75826.
MGI	MGI:1923076. Senp2.
Phylogenomic databases
eggNOG	roNOG06700.
HOGENOM	HBG506560.
HOVERGEN	Q91ZX6.
InParanoid	Q91ZX6.
OMA	IDLRKKC.
OrthoDB	EOG9SR0GC.
PhylomeDB	Q91ZX6.
Gene expression databases
ArrayExpress	Q91ZX6.
Bgee	Q91ZX6.
CleanEx	MM_SENP2.
Genevestigator	Q91ZX6.
GermOnline	ENSMUSG00000022855. Mus musculus.
Family and domain databases
InterPro	IPR003653. Peptidase_C48. [Graphical view]
Pfam	PF02902. Peptidase_C48. 1 hit. [Graphical view]
PROSITE	PS50600. ULP_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	344054.
SOURCE	Search...

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Entry information

Entry name

SENP2_MOUSE

Accession

Primary (citable) accession number: Q91ZX6
Secondary accession number(s): Q544T8, Q811R3, Q9D4Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	November 28, 2002
Last modified:	February 9, 2010
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents