Q91ZX1 (C209A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD209 antigen-like protein A

Alternative name(s):
Dendritic cell-specific ICAM-3-grabbing non-integrin
Short name=DC-SIGN
CD_antigen=CD209

Gene names

Name:	Cd209a
Synonyms:	Cire

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable pathogen-recognition receptor. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. May recognize in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens.
Subcellular location	Membrane; Single-pass type II membrane protein Potential Ref.2.
Tissue specificity	Predominantly expressed in dendritic cells. Detected at very low levels in lung, spleen, lymph nodes and bone marrow. Ref.1 Ref.2
Induction	Down-regulated upon activation of dendritic cells. Ref.2
Sequence similarities	Contains 1 C-type lectin domain.
Caution	In mouse, 5 genes homologous to human CD209/DC-SIGN and CD209L/DC-SIGNR have been identified. Mouse CD209A product was named DC-SIGN by Ref.1 because of its similar expression pattern and chromosomal location in juxtaposition to CD23, but despite of the low sequence similarity.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Lectin Mannose-binding Metal-binding
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell surface Inferred from direct assay PubMed 16569675. Source: MGI integral to membrane Inferred from sequence or structural similarity Ref.1Ref.2. Source: MGI
Molecular_function	mannose binding Inferred from direct assay PubMed 16569675PubMed 16682406. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91ZX1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91ZX1-2)

Also known as: neck-less;

The sequence of this isoform differs from the canonical sequence as follows:
     75-101: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	CD209 antigen-like protein A		PRO_0000046604
Regions
	Topological domain	1 – 51	51	Cytoplasmic Potential
	Transmembrane	52 – 72	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	73 – 238	166	Extracellular Potential
	Domain	115 – 229	115	C-type lectin
Sites
	Metal binding	198	1	Calcium By similarity
	Metal binding	200	1	Calcium By similarity
	Metal binding	202	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	205	1	Calcium By similarity
	Metal binding	216	1	Calcium By similarity
	Metal binding	217	1	Calcium By similarity
Amino acid modifications
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Disulfide bond	108 ↔ 119		By similarity
	Disulfide bond	136 ↔ 228		By similarity
	Disulfide bond	207 ↔ 220		By similarity
Natural variations
	Alternative sequence	75 – 101	27	Missing in isoform 2.		VSP_010067

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 05C025FCF2E63DC2	FASTA	238	27,149
	10 20 30 40 50 60 MSDSKEMGKR QLRPLDEELL TSSHTRHSIK GFGFQTNSGF SSFTGCLVHS QVPLALQVLF 70 80 90 100 110 120 LAVCSVLLVV ILVKVYKIPS SQEENNQMNV YQELTQLKAG VDRLCRSCPW DWTHFQGSCY 130 140 150 160 170 180 FFSVAQKSWN DSATACHNVG AQLVVIKSDE EQNFLQQTSK KRGYTWMGLI DMSKESTWYW 190 200 210 220 230 VDGSPLTLSF MKYWSKGEPN NLGEEDCAEF RDDGWNDTKC TNKKFWICKK LSTSCPSK
	Isoform 2 (neck-less) [UniParc]. Checksum: 1AF2988F95FD9634	FASTA	211	24,055
	10 20 30 40 50 60 MSDSKEMGKR QLRPLDEELL TSSHTRHSIK GFGFQTNSGF SSFTGCLVHS QVPLALQVLF 70 80 90 100 110 120 LAVCSVLLVV ILVKDRLCRS CPWDWTHFQG SCYFFSVAQK SWNDSATACH NVGAQLVVIK 130 140 150 160 170 180 SDEEQNFLQQ TSKKRGYTWM GLIDMSKEST WYWVDGSPLT LSFMKYWSKG EPNNLGEEDC 190 200 210 AEFRDDGWND TKCTNKKFWI CKKLSTSCPS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Five mouse homologues of the human dendritic cell C-type lectin, DC-SIGN." Park C.G., Takahara K., Umemoto E., Yashima Y., Matsubara K., Matsuda Y., Clausen B.E., Inaba K., Steinman R.M. Int. Immunol. 13:1283-1290(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Strain: C57BL/6.
[2]	"Molecular cloning of a C-type lectin superfamily protein differentially expressed by CD8alpha(-) splenic dendritic cells." Caminschi I., Lucas K.M., O'Keeffe M.A., Hochrein H., Laabi Y., Brodnicki T.C., Lew A.M., Shortman K., Wright M.D. Mol. Immunol. 38:365-373(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Oviduct and Spleen.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

SIGNR5

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF373408 mRNA. Translation: AAL13234.1. AF374470 mRNA. Translation: AAL27539.1. AY049062 mRNA. Translation: AAK85825.1. AK054139 mRNA. Translation: BAC35667.1. AK171639 mRNA. Translation: BAE42579.1.
IPI	IPI00131497. IPI00131528.
RefSeq	NP_573501.1. NM_133238.5.
UniGene	Mm.32510.
3D structure databases
ProteinModelPortal	Q91ZX1.
SMR	Q91ZX1. Positions 74-230.
ModBase	Search...
PTM databases
PhosphoSite	Q91ZX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000012847; ENSMUSP00000012847; ENSMUSG00000031494.
GeneID	170786.
KEGG	mmu:170786.
UCSC	uc009ksq.2. mouse. uc012fyt.1. mouse.
Organism-specific databases
CTD	170786.
MGI	MGI:2157942. Cd209a.
Phylogenomic databases
eggNOG	NOG267427.
GeneTree	ENSGT00660000095359.
HOGENOM	HOG000013222.
HOVERGEN	HBG050992.
InParanoid	Q91ZX1.
KO	K06563.
OMA	NDSATAC.
OrthoDB	EOG40K7ZZ.
Gene expression databases
ArrayExpress	Q91ZX1.
Bgee	Q91ZX1.
CleanEx	MM_CD209A.
Genevestigator	Q91ZX1.
GermOnline	ENSMUSG00000031494. Mus musculus.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR018378. C-type_lectin_CS. IPR016187. C-type_lectin_fold. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. 1 hit. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	370415.
SOURCE	Search...

Entry information

Entry name

C209A_MOUSE

Accession

Primary (citable) accession number: Q91ZX1
Secondary accession number(s): Q3TAT9, Q91ZW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program