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Protein

Transcription factor A, mitochondrial

Gene

Tfam

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA (By similarity). Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Intercalates between bases and promotes DNA bendingBy similarity1
Sitei181Intercalates between bases and promotes DNA bendingBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi49 – 117HMG box 1PROSITE-ProRule annotationAdd BLAST69
DNA bindingi154 – 218HMG box 2PROSITE-ProRule annotationAdd BLAST65

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • DNA binding Source: RGD
  • DNA binding, bending Source: UniProtKB
  • DNA binding transcription factor activity Source: RGD
  • mitochondrial promoter sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: Ensembl

GO - Biological processi

  • mitochondrial DNA replication Source: RGD
  • mitochondrial respiratory chain complex assembly Source: Ensembl
  • mitochondrial transcription Source: RGD
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription initiation from mitochondrial promoter Source: UniProtKB

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-163282 Mitochondrial transcription initiation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor A, mitochondrial
Short name:
mtTFA
Gene namesi
Name:Tfam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi620682 Tfam

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Mitochondrion nucleoid, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionSequence analysisAdd BLAST42
ChainiPRO_000001347243 – 244Transcription factor A, mitochondrialAdd BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Phosphoserine; by PKABy similarity1
Modified residuei55Phosphoserine; by PKABy similarity1
Modified residuei60Phosphoserine; by PKABy similarity1
Modified residuei66N6-succinyllysineBy similarity1
Modified residuei121PhosphothreonineBy similarity1
Modified residuei159Phosphoserine; by PKABy similarity1
Modified residuei192PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91ZW1
PRIDEiQ91ZW1

Expressioni

Tissue specificityi

The mitochondrial isoform is widely expressed while the nuclear isoform is testis-specific.

Gene expression databases

BgeeiENSRNOG00000000613
GenevisibleiQ91ZW1 RN

Interactioni

Subunit structurei

Monomer; binds DNA as a monomer. Upon metabolic stress, forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with TFB1M and TFB2M. Interacts with CLPX; this enhances DNA-binding.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000753

Structurei

3D structure databases

ProteinModelPortaliQ91ZW1
SMRiQ91ZW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Binds DNA via its HMG boxes. When bound to the mitochondrial light strand promoter, bends DNA into a U-turn shape, each HMG box bending the DNA by 90 degrees (By similarity).By similarity

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0381 Eukaryota
COG5648 LUCA
GeneTreeiENSGT00440000039001
HOGENOMiHOG000139423
HOVERGENiHBG106674
InParanoidiQ91ZW1
KOiK11830
OMAiQRTKYDA
OrthoDBiEOG091G0SA8
PhylomeDBiQ91ZW1
TreeFamiTF318343

Family and domain databases

Gene3Di1.10.30.10, 2 hits
InterProiView protein in InterPro
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
PfamiView protein in Pfam
PF00505 HMG_box, 1 hit
PF09011 HMG_box_2, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 2 hits
SUPFAMiSSF47095 SSF47095, 2 hits
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Mitochondrial (identifier: Q91ZW1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALFRGMWGV LRTLGRTGVE MCAGCGGRIP SPVSLICIPK CFSSLGNYPK
60 70 80 90 100
KPMSSYLRFS TEQLPKFKAK HPDAKVSELI RKIAAMWREL PEAEKKVYEA
110 120 130 140 150
DFKAEWKVYK EAVSKYKEQL TPSQLMGLEK EARQKRLKKK AQIKRRELIL
160 170 180 190 200
LGKPKRPRSA YNIYVSESFQ EAKDESAQGK LKLVNQAWKN LSHDEKQAYI
210 220 230 240
QLAKDDRIRY DNEMKSWEEQ MAEVGRSDLI RRSVKRPPGD ISEN
Length:244
Mass (Da):28,187
Last modified:December 1, 2001 - v1
Checksum:i8725027A6331D62E
GO
Isoform Nuclear (identifier: Q91ZW1-2)
Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171E → G in BAA77755 (PubMed:10902920).Curated1
Sequence conflicti177A → P in BAA77755 (PubMed:10902920).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014089 mRNA Translation: BAA77755.1
AJ312746 mRNA Translation: CAC84763.1
AF377866 mRNA Translation: AAL12892.1
BC062022 mRNA Translation: AAH62022.1
RefSeqiNP_112616.1, NM_031326.1
UniGeneiRn.18266

Genome annotation databases

EnsembliENSRNOT00000000753; ENSRNOP00000000753; ENSRNOG00000000613 [Q91ZW1-1]
GeneIDi83474
KEGGirno:83474
UCSCiRGD:620682 rat [Q91ZW1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTFAM_RAT
AccessioniPrimary (citable) accession number: Q91ZW1
Secondary accession number(s): Q9WTM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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