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Protein

Plexin domain-containing protein 1

Gene

Plxdc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a critical role in endothelial cell capillary morphogenesis.1 Publication

GO - Molecular functioni

  1. receptor activity Source: InterPro

GO - Biological processi

  1. spinal cord development Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Plexin domain-containing protein 1
Alternative name(s):
Tumor endothelial marker 7
Gene namesi
Name:Plxdc1
Synonyms:Tem7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1919574. Plxdc1.

Subcellular locationi

Cell membrane Curated; Single-pass type I membrane protein Curated. Cell junctiontight junction By similarity
Note: Localized predominantly at the tight junctions of vascular endothelial cells and to a lesser extent at the luminal surface of vascular endothelial cells.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 426407ExtracellularSequence AnalysisAdd
BLAST
Transmembranei427 – 44721HelicalSequence AnalysisAdd
BLAST
Topological domaini448 – 50053CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. dendrite Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. neuronal cell body Source: Ensembl
  5. plasma membrane Source: UniProtKB
  6. receptor complex Source: MGI
  7. tight junction Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 500481Plexin domain-containing protein 1PRO_0000232752Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi318 – 3181N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ91ZV7.

PTM databases

PhosphoSiteiQ91ZV7.

Expressioni

Tissue specificityi

Detected in brain. Highly expressed in Purkinje cells of the cerebellum.1 Publication

Developmental stagei

Expression increases in endothelial cells undergoing capillary morphogenesis.1 Publication

Gene expression databases

BgeeiQ91ZV7.
CleanExiMM_PLXDC1.
ExpressionAtlasiQ91ZV7. baseline and differential.
GenevestigatoriQ91ZV7.

Interactioni

Subunit structurei

Interacts with NID1. May interact with CTTN.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Nid1P084602EBI-8280807,EBI-8280787From a different organism.

Protein-protein interaction databases

IntActiQ91ZV7. 1 interaction.
MINTiMINT-1891999.

Structurei

3D structure databases

SMRiQ91ZV7. Positions 294-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the plexin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG291117.
GeneTreeiENSGT00440000033408.
HOGENOMiHOG000234044.
HOVERGENiHBG079500.
InParanoidiQ91ZV7.
OMAiVEDNHSY.
OrthoDBiEOG712TWH.
PhylomeDBiQ91ZV7.
TreeFamiTF314400.

Family and domain databases

InterProiIPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
[Graphical view]
PfamiPF01437. PSI. 1 hit.
[Graphical view]
SMARTiSM00423. PSI. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91ZV7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAQLWLLQL LLLRGAARAL SPATPAGHNE GQDSAWTAKR TRQGWSRRPR
60 70 80 90 100
ESPAQVLKPG KTQLSQDLGG GSLAIDTLPD NRTRVVEDNH NYYVSRVYGP
110 120 130 140 150
GEKQSQDLWV DLAVANRSHV KIHRILSSSH RQASRVVLSF DFPFYGHPLR
160 170 180 190 200
QITIATGGFI FMGDMLHRML TATQYVAPLM ANFNPGYSDN STVAYFDNGT
210 220 230 240 250
VFVVQWDHVY LQDREDRGSF TFQAALHRDG RIVFGYKEIP MAVLDISSAQ
260 270 280 290 300
HPVKAGLSDA FMILNSSPEV PASQRRTIFE YHRVELDSSK ITTTSAVEFT
310 320 330 340 350
PLPTCLQHQS CDTCVSSNLT FNCSWCHVLQ RCSSGFDRYR QEWLTYGCAQ
360 370 380 390 400
EAEGKTCEDF QDDSHYSASP DSSFSPFNGD STTSSSLFID SLTTEDDTKL
410 420 430 440 450
NPYAEGDGLP DHSSPKSKGP PVHLGTIVGI VLAVLLVAAI ILAGIYISGH
460 470 480 490 500
PNSNAALFFI ERRPHHWPAM KFHNHPNHST YTEVEPSGHE KEGFVEAEQC
Length:500
Mass (Da):55,635
Last modified:January 11, 2011 - v2
Checksum:i802D6865F8CA18BD
GO
Isoform 2 (identifier: Q91ZV7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     86-86: V → VALSLSPQ

Note: No experimental confirmation available.

Show »
Length:507
Mass (Da):56,332
Checksum:iC2D50B44561C3415
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041Q → R in AAI14353 (PubMed:15489334).Curated
Sequence conflicti248 – 2481S → P in AAI14353 (PubMed:15489334).Curated
Sequence conflicti272 – 2721A → E in AAL11997 (PubMed:11559528).Curated
Sequence conflicti272 – 2721A → E in AAI14353 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei86 – 861V → VALSLSPQ in isoform 2. 1 PublicationVSP_017975

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378760 mRNA. Translation: AAL11997.1.
AK010361 mRNA. Translation: BAB26881.1.
AK036144 mRNA. Translation: BAC29318.1.
AK153684 mRNA. Translation: BAE32144.1.
AL591209 Genomic DNA. Translation: CAM20410.1.
AL591209 Genomic DNA. Translation: CAM20411.1.
CH466556 Genomic DNA. Translation: EDL16106.1.
BC114352 mRNA. Translation: AAI14353.1.
CCDSiCCDS25333.1. [Q91ZV7-1]
CCDS48897.1. [Q91ZV7-2]
RefSeqiNP_001157080.1. NM_001163608.1. [Q91ZV7-2]
NP_082475.3. NM_028199.3. [Q91ZV7-1]
UniGeneiMm.39617.
Mm.482750.

Genome annotation databases

EnsembliENSMUST00000017561; ENSMUSP00000017561; ENSMUSG00000017417. [Q91ZV7-1]
ENSMUST00000107565; ENSMUSP00000103191; ENSMUSG00000017417. [Q91ZV7-2]
GeneIDi72324.
KEGGimmu:72324.
UCSCiuc007lfc.2. mouse. [Q91ZV7-1]
uc011ydx.1. mouse. [Q91ZV7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378760 mRNA. Translation: AAL11997.1.
AK010361 mRNA. Translation: BAB26881.1.
AK036144 mRNA. Translation: BAC29318.1.
AK153684 mRNA. Translation: BAE32144.1.
AL591209 Genomic DNA. Translation: CAM20410.1.
AL591209 Genomic DNA. Translation: CAM20411.1.
CH466556 Genomic DNA. Translation: EDL16106.1.
BC114352 mRNA. Translation: AAI14353.1.
CCDSiCCDS25333.1. [Q91ZV7-1]
CCDS48897.1. [Q91ZV7-2]
RefSeqiNP_001157080.1. NM_001163608.1. [Q91ZV7-2]
NP_082475.3. NM_028199.3. [Q91ZV7-1]
UniGeneiMm.39617.
Mm.482750.

3D structure databases

SMRiQ91ZV7. Positions 294-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91ZV7. 1 interaction.
MINTiMINT-1891999.

PTM databases

PhosphoSiteiQ91ZV7.

Proteomic databases

PRIDEiQ91ZV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017561; ENSMUSP00000017561; ENSMUSG00000017417. [Q91ZV7-1]
ENSMUST00000107565; ENSMUSP00000103191; ENSMUSG00000017417. [Q91ZV7-2]
GeneIDi72324.
KEGGimmu:72324.
UCSCiuc007lfc.2. mouse. [Q91ZV7-1]
uc011ydx.1. mouse. [Q91ZV7-2]

Organism-specific databases

CTDi57125.
MGIiMGI:1919574. Plxdc1.

Phylogenomic databases

eggNOGiNOG291117.
GeneTreeiENSGT00440000033408.
HOGENOMiHOG000234044.
HOVERGENiHBG079500.
InParanoidiQ91ZV7.
OMAiVEDNHSY.
OrthoDBiEOG712TWH.
PhylomeDBiQ91ZV7.
TreeFamiTF314400.

Miscellaneous databases

NextBioi336020.
PROiQ91ZV7.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZV7.
CleanExiMM_PLXDC1.
ExpressionAtlasiQ91ZV7. baseline and differential.
GenevestigatoriQ91ZV7.

Family and domain databases

InterProiIPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
[Graphical view]
PfamiPF01437. PSI. 1 hit.
[Graphical view]
SMARTiSM00423. PSI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cell surface tumor endothelial markers are conserved in mice and humans."
    Carson-Walter E.B., Watkins D.N., Nanda A., Vogelstein B., Kinzler K.W., St Croix B.
    Cancer Res. 61:6649-6655(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Embryonic stem cell and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R."
    Nanda A., Buckhaults P., Seaman S., Agrawal N., Boutin P., Shankara S., Nacht M., Teicher B., Stampfl J., Singh S., Vogelstein B., Kinzler K.W., St Croix B.
    Cancer Res. 64:8507-8511(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  7. "Modulation of tumor endothelial cell marker 7 expression during endothelial cell capillary morphogenesis."
    Wang X.Q., Sheibani N., Watson J.C.
    Microvasc. Res. 70:189-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  8. "Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo."
    Lee H.K., Seo I.A., Park H.K., Park H.T.
    FEBS Lett. 580:2253-2257(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NID1.

Entry informationi

Entry nameiPLDX1_MOUSE
AccessioniPrimary (citable) accession number: Q91ZV7
Secondary accession number(s): A2A537
, A2A538, Q29R73, Q8BM20, Q9CWV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: January 11, 2011
Last modified: April 1, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.